Möchten Sie über diese Zeitschrift informiert bleiben? Klicken Sie bitte auf die Buttons, um unsere Alerts zu abonnieren.
Möchten Sie über diese Zeitschrift informiert bleiben? Klicken Sie bitte auf die Buttons, um unsere Alerts zu abonnieren.
Probiotics maintain intestinal secretory immunoglobulin A levels in healthy formula-fed infants: a randomised, double-blind, placebo-controlled study
in Beneficial MicrobesSearch for other papers by L. Xiao in
Current site
Google Scholar
Search for other papers by C. Gong in
Current site
Google Scholar
Search for other papers by Y. Ding in
Current site
Google Scholar
Search for other papers by G. Ding in
Current site
Google Scholar
Search for other papers by X. Xu in
Current site
Google Scholar
Search for other papers by C. Deng in
Current site
Google Scholar
Search for other papers by X. Ze in
Current site
Google Scholar
Search for other papers by P. Malard in
Current site
Google Scholar
Search for other papers by X. Ben in
Current site
Google Scholar
Formula-fed infants are more susceptible to infectious diseases because they lack the maternal immune factors transferred from breast milk, while their own immune system is still immature. As timely probiotic administration was suggested to promote immune system development in formula-fed infants, this study aimed at assessing the safety and the effects of a probiotic supplement (Bifidobacterium infantis R0033, Bifidobacterium bifidum R0071, and Lactobacillus helveticus R0052) on mucosal immune competence and digestive function in formula-fed infants. Healthy infants (3.5-6 months old) were randomised to receive either probiotic- (n=66) or placebo-supplemented (n=66) formula once a day for four weeks. In the probiotics group, faecal secretory immunoglobulin A (SIgA) levels remained similar between visit 2 (baseline; V2) and visit 3 (end-of-treatment; V3), but decreased in the placebo group. Changes in SIgA levels following treatment (log10ΔV3-V2 [95%CI]) between the probiotic and placebo groups were statistically significant (23 ng/dl [-57;102] and -137 ng/dl [-212;-62], respectively (P=0.0044; ANCOVA)). While log10ΔV3-V2 [95%CI] for salivary SIgA levels increased in both groups, this trend was more pronounced in the probiotics than in the placebo group with an increase of 123 ng/dl [9;236] and 37 ng/dL [-72;147], respectively (P=0.2829; ANCOVA). The weekly average number of stools/day was significantly higher in the probiotics group compared to placebo during the last week of treatment for the per protocol population. There was no difference in microbiota composition or anthropometric parameters between groups. No serious adverse event was reported, and all adverse events were mild and unrelated to the product or study. Our results show that formula-fed infants receiving probiotics maintained higher faecal SIgA levels at the end of the four-week treatment period, suggesting a positive effect of probiotics on SIgA production. This study demonstrates the safety of this probiotic formulation in infants. Formula-fed infants may benefit from probiotics supplementation to sustain the development of mucosal immunity.
-
Aloisio, I., Prodam, F., Giglione, E., Bozzi Cionci, N., Solito, A., Bellone, S., Baffoni, L., Mogna, L., Pane, M., Bona, G. and Di Gioia, D., 2018. Three-month feeding integration with Bifidobacterium strains prevents gastrointestinal symptoms in healthy newborns. Frontiers in Nutrition 5: 39-39. DOI: https://doi.org/10.3389/fnut.2018.00039
-
Bakker-Zierikzee, A., Tol, E., Kroes, H., Alles, M., Kok, F. and Bindels, J., 2006. Faecal SIgA secretion in infants fed on pre- or probiotic infant formula. Pediatric Allergy and Immunology 17: 134-140.
'Faecal SIgA secretion in infants fed on pre- or probiotic infant formula ' () 17 Pediatric Allergy and Immunology : 134 -140.
-
Booth, C.K., Dwyer, D.B., Pacque, P.F. and Ball, M.J., 2009. Measurement of immunoglobulin A in saliva by particle-enhanced nephelometric immunoassay: sample collection, limits of quantitation, precision, stability and reference range. Annals of Clinical Biochemistry 46: 401-406. DOI: https://doi.org/10.1258/acb.2009.008248
-
Cacho, N.T. and Lawrence, R.M., 2017. Innate immunity and breast milk. Frontiers in Immunology 8: 584. DOI: https://doi.org/10.3389/fimmu.2017.00584
-
Cazzola, M., Pham-Thi, N., Kerihuel, J.C., Durand, H. and Bohbot, S., 2010a. Efficacy of a synbiotic supplementation in the prevention of common winter diseases in children: a randomized, double-blind, placebo-controlled pilot study. Therapeutic Advances in Respiratory Disease 4: 271-278. DOI: https://doi.org/10.1177/1753465810379010
-
Cazzola, M., Tompkins, T.A. and Matera, M.G., 2010b. Immunomodulatory impact of a synbiotic in T(h)1 and T(h)2 models of infection. Therapeutic Advances in Respiratory Disease 4: 259-270. DOI: https://doi.org/10.1177/1753465810379009
-
Chen, L., Ouyang, J., Liao, W. and Zhang, W., 2007. Effect of synbiotics probiotics on improving mucosal immunity in children’s digestive tract. Chinese Journal of Microecology 2: 137-141. [in Chinese]
'Effect of synbiotics probiotics on improving mucosal immunity in children’s digestive tract ' () 2 Chinese Journal of Microecology : 137 -141.
-
De Andres, J., Manzano, S., Garcia, C., Rodriguez, J.M., Espinosa-Martos, I. and Jimenez, E., 2018. Modulatory effect of three probiotic strains on infants’ gut microbial composition and immunological parameters on a placebo-controlled, double-blind, randomised study. Beneficial Microbes 9: 573-584. DOI: https://doi.org/10.3920/bm2017.0132
-
Deshpande, G., Jape, G., Rao, S. and Patole, S., 2017. Benefits of probiotics in preterm neonates in low-income and medium-income countries: a systematic review of randomised controlled trials. BMJ 7: e017638. DOI: https://doi.org/10.1136/bmjopen-2017-017638
-
Dion, C., Montagne, P., Bene, M.C. and Faure, G., 2004. Measurement of faecal immunoglobulin a levels in young children. Journal of Clinical Laboratory Analysis 18: 195-199. DOI: https://doi.org/10.1002/jcla.20022
-
Easo, J.G., Measham, J.D, Munroe, J. and Green-Johnson, J., 2002. Immunostimulatory actions of lactobacilli: mitogenic induction of antibody production and spleen cell proliferation by Lactobacillus delbrueckii subsp. bulgaricus and Lactobacillus acidophilus. Food and Agricultural Immunology 14: 73-83. DOI: https://doi.org/10.1080/09540100220137682
-
Ewaschuk, J.B., Diaz, H., Meddings, L., Diederichs, B., Dmytrash, A., Backer, J., Looijer-van Langen, M. and Madsen, K.L., 2008. Secreted bioactive factors from Bifidobacterium infantis enhance epithelial cell barrier function. American Journal of Physiology – Gastrointestinal and Liver Physiology 295: G1025-G1034. DOI: https://doi.org/10.1152/ajpgi.90227.2008
-
Fan, W., Huo, G., Li, X., Yang, L., Duan, C., Wang, T. and Chen, J., 2013. Diversity of the intestinal microbiota in different patterns of feeding infants by Illumina high-throughput sequencing. World Journal of Microbiology and Biotechnology 29: 2365-2372. DOI: https://doi.org/10.1007/s11274-013-1404-3
-
Gensollen, T., Iyer, S.S., Kasper, D.L. and Blumberg, R.S., 2016. How colonization by microbiota in early life shapes the immune system. Science 352: 539-544. DOI: https://doi.org/10.1126/science.aad9378
-
Giovannini, M., Verduci, E., Gregori, D., Ballali, S., Soldi, S., Ghisleni, D., Riva, E. and Group P.T.S., 2014. Prebiotic effect of an infant formula supplemented with galacto-oligosaccharides: randomized multicenter trial. Journal of the American College of Nutrition 33: 385-393. DOI: https://doi.org/10.1080/07315724.2013.878232
-
Guaraldi, F. and Salvatori, G., 2012. Effect of breast and formula feeding on gut microbiota shaping in newborns. Frontiers in Cellular and Infection Microbiology 2: 94. DOI: https://doi.org/10.3389/fcimb.2012.00094
-
Jacobs, S.E., Tobin, J.M., Opie, G.F., Donath, S., Tabrizi, S.N., Pirotta, M., Morley, C.J. and Garland, S.M., 2013. Probiotic effects on late-onset sepsis in very preterm infants: a randomized controlled trial. Pediatrics 132: 1055-1062. DOI: https://doi.org/10.1542/peds.2013-1339
-
Jakaitis, B.M. and Denning, P.W., 2014. Human breast milk and the gastrointestinal innate immune system. Clinics in Perinatology 41: 423-435. DOI: https://doi.org/10.1016/j.clp.2014.02.011
-
Kato, L.M., Kawamoto, S., Maruya, M. and Fagarasan, S., 2014. The role of the adaptive immune system in regulation of gut microbiota. Immunological Reviews 260: 67-75. DOI: https://doi.org/10.1111/imr.12185
-
Kawashima, T., Ikari, N., Kouchi, T., Kowatari, Y., Kubota, Y., Shimojo, N. and Tsuji, N.M., 2018. The molecular mechanism for activating IgA production by Pediococcus acidilactici K15 and the clinical impact in a randomized trial. Scientific Reports 8: 5065. DOI: https://doi.org/10.1038/s41598-018-23404-4
-
Lee, S.A., Lim, J.Y., Kim, B.-S., Cho, S.J., Kim, N.Y., Kim, O.B. and Kim, Y., 2015. Comparison of the gut microbiota profile in breast-fed and formula-fed Korean infants using pyrosequencing. Nutrition Research and Practice 9: 242-248. DOI: https://doi.org/10.4162/nrp.2015.9.3.242
-
Lewis, D.B. and Wilson, C.B., 2011. Developmental immunology and role of host defenses in fetal and neonatal susceptibility to infection. Infectious diseases of the fetus and newborn. Elsevier, New York, NY, USA, pp. 80-191. DOI: https://doi.org/10.1016/b978-1-4160-6400-8.00004-3
-
MacPherson, C., Audy, J., Mathieu, O. and Tompkins, T.A., 2014. Multistrain probiotic modulation of intestinal epithelial cells’ immune response to a double-stranded RNA ligand, poly(i.c). Applied and Environmental Microbiology 80: 1692-1700. DOI: https://doi.org/10.1128/aem.03411-13
-
MacPherson, C.W., Shastri, P., Mathieu, O., Tompkins, T.A. and Burguiere, P., 2017. Genome-wide immune modulation of TLR3-mediated inflammation in intestinal epithelial cells differs between single and multi-strain probiotic combination. PLoS ONE 12: e0169847. DOI: https://doi.org/10.1371/journal.pone.0169847
-
Mantis, N.J., Rol, N. and Corthesy, B., 2011. Secretory IgA’s complex roles in immunity and mucosal homeostasis in the gut. Mucosal Immunology 4: 603-611. DOI: https://doi.org/10.1038/mi.2011.41
-
Manzano, S., De Andres, J., Castro, I., Rodriguez, J.M., Jimenez, E. and Espinosa-Martos, I., 2017. Safety and tolerance of three probiotic strains in healthy infants: a multi-centre randomized, double-blind, placebo-controlled trial. Beneficial Microbes 8: 569-578. DOI: https://doi.org/10.3920/bm2017.0009
-
Maruyama, K., Hida, M., Kohgo, T. and Fukunaga, Y., 2009. Changes in salivary and fecal secretory IgA in infants under different feeding regimens. Pediatrics International 51: 342-345. DOI: https://doi.org/10.1111/j.1442-200X.2008.02748.x
-
Penders, J., Vink, C., Driessen, C., London, N., Thijs, C. and Stobberingh, E.E., 2005. Quantification of Bifidobacterium spp., Escherichia coli and Clostridium difficile in faecal samples of breast-fed and formula-fed infants by real-time PCR. FEMS Microbiology Letters 243: 141-147. DOI: https://doi.org/10.1016/j.femsle.2004.11.052
-
Rautava, S., Arvilommi, H. and Isolauri, E., 2006. Specific probiotics in enhancing maturation of IgA responses in formula-fed infants. Pediatric Research 60: 221-224. DOI: https://doi.org/10.1203/01.pdr.0000228317.72933.db
-
Rodríguez, J.M., Murphy, K., Stanton, C., Ross, R.P., Kober, O.I., Juge, N., Avershina, E., Rudi, K., Narbad, A., Jenmalm, M.C., Marchesi, J.R. and Collado, M.C., 2015. The composition of the gut microbiota throughout life, with an emphasis on early life. Microbial Ecology in Health and Disease 26: 26050-26050. DOI: https://doi.org/10.3402/mehd.v26.26050
-
Scholtens, P.A., Alliet, P., Raes, M., Alles, M.S., Kroes, H., Boehm, G., Knippels, L.M., Knol, J. and Vandenplas, Y., 2008. Fecal secretory immunoglobulin A is increased in healthy infants who receive a formula with short-chain galacto-oligosaccharides and long-chain fructo-oligosaccharides. Journal of Nutrition 138: 1141-1147. DOI: https://doi.org/10.1093/jn/138.6.1141
-
Simeoni, U., Berger, B., Junick, J., Blaut, M., Pecquet, S., Rezzonico, E., Grathwohl, D., Sprenger, N., Brussow, H., Study, T., Szajewska, H., Bartoli, J.M., Brevaut-Malaty, V., Borszewska-Kornacka, M., Feleszko, W., Francois, P., Gire, C., Leclaire, M., Maurin, J.M., Schmidt, S., Skorka, A., Squizzaro, C. and Verdot, J.J., 2016. Gut microbiota analysis reveals a marked shift to bifidobacteria by a starter infant formula containing a synbiotic of bovine milk-derived oligosaccharides and Bifidobacterium animalis subsp. lactis CNCM I-3446. Environmental Microbiology 18: 2185-2195. DOI: https://doi.org/10.1111/1462-2920.13144
-
Skorka, A., Piescik-Lech, M., Kolodziej, M. and Szajewska, H., 2017. To add or not to add probiotics to infant formulae? An updated systematic review. Beneficial Microbes 8: 717-725. DOI: https://doi.org/10.3920/bm2016.0233
-
Stojkovic, A., Simovic, A., Bogdanovic, Z., Bankovic, D. and Poskurica, M., 2016. Clinical trial/experimental study (consort compliant): optimal time period to achieve the effects on synbiotic-controlled wheezing and respiratory infections in young children. Srpski Arhiv za Celokupno Lekarstvo 144: 38-45.
'Clinical trial/experimental study (consort compliant): optimal time period to achieve the effects on synbiotic-controlled wheezing and respiratory infections in young children ' () 144 Srpski Arhiv za Celokupno Lekarstvo : 38 -45.
-
Thomas, J.P., Raine, T., Reddy, S. and Belteki, G., 2017. Probiotics for the prevention of necrotising enterocolitis in very low-birth-weight infants: a meta-analysis and systematic review. Acta Paediatrica 106: 1729-1741. DOI: https://doi.org/10.1111/apa.13902
-
Tzira, D., Prezerakou, A., Papadatos, I., Vintila, A., Bartzeliotou, A., Apostolakou, F., Papassotiriou, I. and Papaevangelou, V., 2018. Salivary biomarkers may measure stress responses in critically ill children. SAGE Open Medicine 6: 2050312118802452. DOI: https://doi.org/10.1177/2050312118802452
-
Vandenplas, Y., Analitis, A., Tziouvara, C., Kountzoglou, A., Drakou, A., Tsouvalas, M., Mavroudi, A. and Xinias, I., 2017. Safety of a new synbiotic starter formula. Pediatric Gastroenterology, Hepatology and Nutrition 20: 167-177. DOI: https://doi.org/10.5223/pghn.2017.20.3.167
-
Wang, M., Li, M., Wu, S., Lebrilla, C.B., Chapkin, R.S., Ivanov, I. and Donovan, S.M., 2015. Fecal microbiota composition of breast-fed infants is correlated with human milk oligosaccharides consumed. Journal of Pediatric Gastroenterology and Nutrition 60: 825-833. DOI: https://doi.org/10.1097/MPG.0000000000000752
-
World Health Organisation (WHO), 2018a. Exclusive breastfeeding for optimal growth, development and health of infants. e-Library of Evidence for Nutrition Actions (eLENA). Available at: https://www.who.int/elena/titles/exclusive_breastfeeding/en/
-
World Health Organisation (WHO), 2018b. Preterm birth fact sheet. Available at: https://www.who.int/en/news-room/fact-sheets/detail/preterm-birth
-
Wold, A.E. and Adlerberth, I., 2000. Breast feeding and the intestinal microflora of the infant – implications for protection against infectious diseases. Advances in Experimental Medicine and Biology 478: 77-93. DOI: https://doi.org/10.1007/0-306-46830-1_7
-
Wopereis, H., Oozeer, R., Knipping, K., Belzer, C. and Knol, J., 2014. The first thousand days – intestinal microbiology of early life: establishing a symbiosis. Pediatric Allergy and Immunology 25: 428-438. DOI: https://doi.org/10.1111/pai.12232
-
Xiao, L., Ding, G., Ding, Y., Deng, C., Ze, X., Chen, L., Zhang, Y., Song, L., Yan, H., Liu, F. and Ben, X., 2017. Effect of probiotics on digestibility and immunity in infants: a study protocol for a randomized controlled trial. Medicine 96: e5953. DOI: https://doi.org/10.1097/md.0000000000005953
-
Yatsunenko, T., Rey, F.E., Manary, M.J., Trehan, I., Dominguez-Bello, M.G., Contreras, M., Magris, M., Hidalgo, G., Baldassano, R.N., Anokhin, A.P., Heath, A.C., Warner, B., Reeder, J., Kuczynski, J., Caporaso, J.G., Lozupone, C.A., Lauber, C., Clemente, J.C., Knights, D., Knight, R. and Gordon, J.I., 2012. Human gut microbiome viewed across age and geography. Nature 486: 222-227. DOI: https://doi.org/10.1038/nature11053
-
Zhang, G.-Q., Hu, H.-J., Liu, C.-Y., Shakya, S. and Li, Z.-Y., 2016. Probiotics for preventing late-onset sepsis in preterm neonates: a PRISMA-compliant systematic review and meta-analysis of randomized controlled trials. Medicine 95: e2581-e2581. DOI: https://doi.org/10.1097/MD.0000000000002581
| Insgesamt | Letzte 365 Tage | In den letzten 30 Tagen | |
|---|---|---|---|
| Aufrufe von Kurzbeschreibungen | 0 | 0 | 0 |
| Gesamttextansichten | 1139 | 421 | 26 |
| PDF-Downloads | 1015 | 421 | 17 |
Probiotics maintain intestinal secretory immunoglobulin A levels in healthy formula-fed infants: a randomised, double-blind, placebo-controlled study
in Beneficial MicrobesSearch for other papers by L. Xiao in
Current site
Google Scholar
PubMed
Search for other papers by C. Gong in
Current site
Google Scholar
PubMed
Search for other papers by Y. Ding in
Current site
Google Scholar
PubMed
Search for other papers by G. Ding in
Current site
Google Scholar
PubMed
Search for other papers by X. Xu in
Current site
Google Scholar
PubMed
Search for other papers by C. Deng in
Current site
Google Scholar
PubMed
Search for other papers by X. Ze in
Current site
Google Scholar
PubMed
Search for other papers by P. Malard in
Current site
Google Scholar
PubMed
Search for other papers by X. Ben in
Current site
Google Scholar
PubMed
Formula-fed infants are more susceptible to infectious diseases because they lack the maternal immune factors transferred from breast milk, while their own immune system is still immature. As timely probiotic administration was suggested to promote immune system development in formula-fed infants, this study aimed at assessing the safety and the effects of a probiotic supplement (Bifidobacterium infantis R0033, Bifidobacterium bifidum R0071, and Lactobacillus helveticus R0052) on mucosal immune competence and digestive function in formula-fed infants. Healthy infants (3.5-6 months old) were randomised to receive either probiotic- (n=66) or placebo-supplemented (n=66) formula once a day for four weeks. In the probiotics group, faecal secretory immunoglobulin A (SIgA) levels remained similar between visit 2 (baseline; V2) and visit 3 (end-of-treatment; V3), but decreased in the placebo group. Changes in SIgA levels following treatment (log10ΔV3-V2 [95%CI]) between the probiotic and placebo groups were statistically significant (23 ng/dl [-57;102] and -137 ng/dl [-212;-62], respectively (P=0.0044; ANCOVA)). While log10ΔV3-V2 [95%CI] for salivary SIgA levels increased in both groups, this trend was more pronounced in the probiotics than in the placebo group with an increase of 123 ng/dl [9;236] and 37 ng/dL [-72;147], respectively (P=0.2829; ANCOVA). The weekly average number of stools/day was significantly higher in the probiotics group compared to placebo during the last week of treatment for the per protocol population. There was no difference in microbiota composition or anthropometric parameters between groups. No serious adverse event was reported, and all adverse events were mild and unrelated to the product or study. Our results show that formula-fed infants receiving probiotics maintained higher faecal SIgA levels at the end of the four-week treatment period, suggesting a positive effect of probiotics on SIgA production. This study demonstrates the safety of this probiotic formulation in infants. Formula-fed infants may benefit from probiotics supplementation to sustain the development of mucosal immunity.
| Insgesamt | Letzte 365 Tage | In den letzten 30 Tagen | |
|---|---|---|---|
| Aufrufe von Kurzbeschreibungen | 0 | 0 | 0 |
| Gesamttextansichten | 1139 | 421 | 26 |
| PDF-Downloads | 1015 | 421 | 17 |
