Individual and combined effects of feed artificially contaminated with with fumonisin B1 and T-2 toxin in weaned rabbits
äºWorld Mycotoxin JournalSearch for other papers by D. Hafner in
Current site
Google Scholar
Search for other papers by A. Szabó in
Current site
Google Scholar
Search for other papers by L. DâCosta in
Current site
Google Scholar
Search for other papers by J. Szabó-Fodor in
Current site
Google Scholar
Search for other papers by G. Tornyos in
Current site
Google Scholar
Search for other papers by Zs. Blochné Bodnár in
Current site
Google Scholar
Search for other papers by K. Ãlbeiné Horvatovich in
Current site
Google Scholar
Search for other papers by E. Baloghné Zándoki in
Current site
Google Scholar
Search for other papers by B. Bóta in
Current site
Google Scholar
MTA-KE Mycotoxins in the Food Chain Research Group, Faculty of Agricultural and Environmental Sciences, Kaposvár University, Guba S. 40., Kaposvár 7400, Hungary.
Search for other papers by M. Kovács in
Current site
Google Scholar
Co-contamination of feed and feed raw materials with two or more mycotoxins is frequently reported, however, only a few studies have investigated the combined effects of low doses of multiple mycotoxins. In the present study the individual and combined effects of 10 mg/kg fumonisin B1 and 2 mg/kg T-2 toxin (n=12/group) were investigated in weaned rabbits. Mycotoxin contaminated feed was produced by adding fungal cultures ofFusarium verticillioides andFusarium sporotrichioides, and fed to 40 days old rabbits during 28 days. Feed intake and body weight were measured weekly, serum biochemistry and antioxidant parameters on day 0, 14 and 28, while histopathological examination and comet assay were performed at the end of the experiment. T-2 exposure both alone and in combination resulted in 15-18% less final body weight compared to the control and FB1 treatment. There was a significant increase in the concentration of plasma total protein, albumin, fructosamine and creatinine in the group treated with FB1 compared to the control. The liver and the kidney of most animals treated with T-2 toxin, FB1 and their combination showed pathological changes, occurring more frequent in animals exposed to both toxins. T-2 resulted in depletion of lymphocytes in the spleen. FB1 and T-2 exerted synergistic effect on the antioxidant/oxidative parameters after 2 weeks of exposure, manifesting in less glutathione and glutathione peroxidase, while more malondialdehyde was produced. Both toxins caused DNA damage in the lymphocytes, which was more pronounced in the group fed T-2 toxin and T-2 combined with FB1, without additive or synergistic effects.
Purchase
Buy instant access (PDF download and unlimited online access):
Institutional Login
Log in with Open Athens, Shibboleth, or your institutional credentials
Personal login
Log in with your brill.com account
-
Bennett, J.W. and Klich, M., 2003. Mycotoxins. Clinical Microbiology Reviews 16: 497-516.
'Mycotoxins ' () 16 Clinical Microbiology Reviews : 497 -516.
-
Dalcero, A., Magnoli, C., Hallak, C., Chiacchiera, S.M., Palacio, G. and Rosa, C.A.R., 2002. Detection of ochratoxin A in animal feed and capacity to produce this mycotoxin byAspergillus sectionNigri in Argentina. Food Additives and Contaminants 19: 1065-1072.
'Detection of ochratoxin A in animal feed and capacity to produce this mycotoxin byAspergillus sectionNigri in Argentina ' () 19 Food Additives and Contaminants : 1065 -1072.
-
El Golli-Bennour, E. and Bacha, H., 2011. Hsp70 expression as biomarkers of oxidative stress: mycotoxinsâ exploration. Toxicology 287: 1-7.
'Hsp70 expression as biomarkers of oxidative stress: mycotoxinsâ exploration ' () 287 Toxicology : 1 -7.
-
European Commission (EC), 2006. Commission recommendation of 17 August 2006 on the presence of deoxynivalenol, zearalenone, ochratoxin A, T-2 and HT-2 and fumonisins in products intended for animal feeding. No. 2006/576/EC, 17 August 2006. Official Journal of the European Union L 229: 7-9.
-
European Commission (EC), 2013. Commission recommendation of 27 March 2013 on the presence of T-2 and HT-2 toxin in cereals and cereal products No. 2013/165/EU, 27 March 2013. Official Journal of the European Union L 91: 12-15.
-
European Food Safety Authority (EFSA), 2005. Opinion of the scientific panel on contaminants in food chain on a request from the commission related to fumonisins as undesirable substances in animal feed. EFSA Journal 235: 1-32.
-
European Food Safety Authority (EFSA), 2014. Scientific opinion on the risk for human and animal health related to the presence of modified forms of certain mycotoxins in food and feed. EFSA Journal 12: 3916.
-
Ewuola, E.O., 2009. Organ traits and histopathology of rabbits fed varied levels of dietary fumonisin B1. Journal of Animal Physiology and Animal Nutrition 93: 726-731.
'Organ traits and histopathology of rabbits fed varied levels of dietary fumonisin B1 ' () 93 Journal of Animal Physiology and Animal Nutrition : 726 -731.
-
Ewuola, E.O., Gbore, F.A., Ogunlade, J.T., Bandyopadhyay, R., Niezen, J. and Egbunike, G.N., 2008. Physiological response of rabbit bucks to dietary fumonisin: performance, haematology and serum biochemistry. Mycopathologia 165: 99-104.
'Physiological response of rabbit bucks to dietary fumonisin: performance, haematology and serum biochemistry ' () 165 Mycopathologia : 99 -104.
-
Fekete, S., Tamás, J., Ványi, A., Glávits, R. and Bata, Ã., 1989. Effect of T-2 toxin on feed intake, digestion and pathology of rabbits. Laboratory Animal Science 39: 603-606.
'Effect of T-2 toxin on feed intake, digestion and pathology of rabbits ' () 39 Laboratory Animal Science : 603 -606.
-
Fekete, S. and Huszenicza, Gy., 1993. Effects of T-2 toxin on ovarian activity and some metabolic variables of rabbits. Laboratory Animal Science 43: 646-649.
'Effects of T-2 toxin on ovarian activity and some metabolic variables of rabbits ' () 43 Laboratory Animal Science : 646 -649.
-
Fodor, J., Kametler, L. and Kovács, M., 2006. Practical aspects of fumonisin production under laboratory conditions. Mycotoxin Research 22: 211-216.
'Practical aspects of fumonisin production under laboratory conditions ' () 22 Mycotoxin Research : 211 -216.
-
FrankiÄ, T., Salobir, J. and Rezar, V., 2008. The effect of vitamin E supplementation on reduction of lymphocyte DNA damage induced by T-2 toxin and deoxynivalenol in weaned pigs. Animal Feed Science and Technology 141: 274-286.
'The effect of vitamin E supplementation on reduction of lymphocyte DNA damage induced by T-2 toxin and deoxynivalenol in weaned pigs ' () 141 Animal Feed Science and Technology : 274 -286.
-
Galvano, F., Russo, A., Cardile, V., Galvano, G., Vanella, A. and Renis, M., 2002. DNA damage in human fibroblasts exposed to fumonisin B1. Food and Chemical Toxicology 40: 25-31.
'DNA damage in human fibroblasts exposed to fumonisin B1 ' () 40 Food and Chemical Toxicology : 25 -31.
-
Gbore, F.A. and Akele, O., 2010. Growth performance, haematology and serum biochemistry of female rabbits (Oryctolagus cuniculus) fed dietary fumonisin. Veterinarski Archiv 80: 431-443.
'Growth performance, haematology and serum biochemistry of female rabbits (Oryctolagus cuniculus) fed dietary fumonisin ' () 80 Veterinarski Archiv : 431 -443.
-
Greco, M.V., Pardo, A.G., Ludemann, V., Martino, P.E. and Pose, G.N., 2012. Mycoflora and natural incidence of selected mycotoxins in rabbit and chinchilla feeds. Scientific World Journal 2012: 956056.
'Mycoflora and natural incidence of selected mycotoxins in rabbit and chinchilla feeds ' () 2012 Scientific World Journal : 956056.
-
Glávits, R., Ványi, A., Fekete, S. and Tamás, J., 1989. Acute toxicological experiment of T-2 toxin in rabbits. Acta Veterinaria Hungarica 37: 75-79.
'Acute toxicological experiment of T-2 toxin in rabbits ' () 37 Acta Veterinaria Hungarica : 75 -79.
-
Grenier, B. and Oswald, I.P., 2011. Mycotoxin co-contamination of food and feed: meta-analysis of publications describing toxicological interactions. World Mycotoxin Journal 4: 285-313.
'Mycotoxin co-contamination of food and feed: meta-analysis of publications describing toxicological interactions ' () 4 World Mycotoxin Journal : 285 -313.
-
Gumprecht, L.A., Marcucci, A., Weigel, R.M., Vesonder, R.F., Riley, R.T., Showker, J.L., Beasley, V.R. and Haschek, W.M. 1995. Effects of intravenous fumonisin B1 in rabbits: nephrotoxicity and sphingolipid alteration. Natural Toxins 3: 395-403.
'Effects of intravenous fumonisin B1 in rabbits: nephrotoxicity and sphingolipid alteration ' () 3 Natural Toxins : 395 -403.
-
Gutleb, A.C., Morrison, E. and Murk, A.J., 2002. Cytotoxicity assays for mycotoxins produced by Fusarium strains: a review. Environmental Toxicology and Pharmacology 11: 309-320.
'Cytotoxicity assays for mycotoxins produced by Fusarium strains: a review ' () 11 Environmental Toxicology and Pharmacology : 309 -320.
-
Harcourt-Brown, F., 2002. Textbook of rabbit medicine. Elsevier, Oxford, UK, 410 pp.
'Textbook of rabbit medicine ', () 410.
-
Horvatovich, K., Hafner, D., Bodnár, Zs., Berta, G., Hancz, Cs., Dutoon, M. and Kovács, M., 2013. Dose-related genotoxic effect of T-2 toxin measured by comet assay using peripherial blood mononuclear cells of healthy pigs. Acta Veterinaria Hungarica 61: 175-186.
'Dose-related genotoxic effect of T-2 toxin measured by comet assay using peripherial blood mononuclear cells of healthy pigs ' () 61 Acta Veterinaria Hungarica : 175 -186.
-
Jaradat, Z.W., Vila, B. and Marquardt, R.R., 2006. Adverse effects of T-2 toxin in chicken lymphocytes blastogenesis and its protection with vitamin E. Toxicology 225: 90-96.
'Adverse effects of T-2 toxin in chicken lymphocytes blastogenesis and its protection with vitamin E ' () 225 Toxicology : 90 -96.
-
Kovács, M., Tornyos, G., Matics, Zs., Mézes, M., Balogh, K., Rajli, V., Bloch-Bodnár, Zs., Rusvai, M., Mándoki, M. and Cseh, S., 2013. Effect of chronic T-2 toxin exposure in rabbit bucks, determination of the no observed adverse effect level (NOAEL). Animal Reproduction Science 13: 245-252.
'Effect of chronic T-2 toxin exposure in rabbit bucks, determination of the no observed adverse effect level (NOAEL) ' () 13 Animal Reproduction Science : 245 -252.
-
Kubena, L.F., Edrington, T.S., Harvey, R.B., Buckley, S.A., Phillips, T.D., Rottinghaus, G.E. and Caspers, H.H., 1997. Individual and combined effects of fumonisin B1 present inFusarium moniliforme culture material and T-2 toxin or deoxynivalenol in broiler chicks. Poultry Science 76: 1239-1247.
'Individual and combined effects of fumonisin B1 present inFusarium moniliforme culture material and T-2 toxin or deoxynivalenol in broiler chicks ' () 76 Poultry Science : 1239 -1247.
-
Kubena, L.F., Edrington, T.S., Kamps-Holtzapple, C., Harvey, R.B., Elissalde, M.H. and Rottinghaus, G.E., 1995. Influence of fumonisin B1 present inFusarium moniliforme culture material and T-2 toxin in turkey poults. Poultry Science 74: 306-313.
'Influence of fumonisin B1 present inFusarium moniliforme culture material and T-2 toxin in turkey poults ' () 74 Poultry Science : 306 -313.
-
Lawrence, R.A. and Burk, R.F., 1978. Species, tissues and subcellular distribution of non-selenium dependent glutathione peroxidase activity. Journal of Nutrition 108: 211-215.
'Species, tissues and subcellular distribution of non-selenium dependent glutathione peroxidase activity ' () 108 Journal of Nutrition : 211 -215.
-
Lee, J.E., 2015. Alternative biomarkers for assessing glycemic control in diabetes: fructosamine, glycated albumin, and 1,5-anhydroglucitol. Annals of Pediatric Endocrinology and Metabolism 20: 74-78.
'Alternative biomarkers for assessing glycemic control in diabetes: fructosamine, glycated albumin, and 1,5-anhydroglucitol ' () 20 Annals of Pediatric Endocrinology and Metabolism : 74 -78.
-
Mary, V.S., Theumer, M.G., Arias, S.L. and Rubinstein, H.R., 2012. Reactive oxygen species sources and biomolecular oxidative damage induced by aflatoxin B1 and fumonisin B1 in rat spleen mononuclear cells. Toxicology 302: 299-307.
'Reactive oxygen species sources and biomolecular oxidative damage induced by aflatoxin B1 and fumonisin B1 in rat spleen mononuclear cells ' () 302 Toxicology : 299 -307.
-
Mead, J.F., Alfin-Slater, R.B., Howton, D.R. and Popják, G., 1986. Lipids: chemistry, biochemistry and nutrition. Springer, New York, NY, USA.
'Lipids: chemistry, biochemistry and nutrition', ().
-
Mohanamba, T., Rao, M.R. and Habibi, S.M.M., 2007. Aflatoxin contamination in animal feed. Indian Veterinary Journal 84: 416.
'Aflatoxin contamination in animal feed ' () 84 Indian Veterinary Journal : 416.
-
Organisation for Economic Co-operation and Development (OECD). Principles of good laboratory practice (as revised in 1997), 1998. OECD Environmental Health and Safety Publications 1. Paris, France.
-
Orsi, R.B., Dilkin, P., Xavier, J.G., Aquino, S., Rocha, L.O. and Corrêa, B., 2009. Acute toxicity of a single gavage dose of fumonisin B1 in rabbits. Chemico-Biological Interactions 179: 351-355.
'Acute toxicity of a single gavage dose of fumonisin B1 in rabbits ' () 179 Chemico-Biological Interactions : 351 -355.
-
Orsi, R.B., Oliveira, C.A.F., Dilkin, P., Xavier, J.G., Direito, G.M. and Corrêa, B., 2007. Effects of oral administration of aflatoxin B1 and fumonisin B1 in rabbits (Oryctolagus cuniculus). Chemico-Biological Interactions 170: 201-208.
'Effects of oral administration of aflatoxin B1 and fumonisin B1 in rabbits (Oryctolagus cuniculus) ' () 170 Chemico-Biological Interactions : 201 -208.
-
Placer, Z.A., Cushman, L.L. and Johnson, B.C., 1966. Estimation of product of lipid peroxidation (malonyl dialdehyde) in biochemical systems. Analytical Biochemistry 16: 359-364.
'Estimation of product of lipid peroxidation (malonyl dialdehyde) in biochemical systems ' () 16 Analytical Biochemistry : 359 -364.
-
Poersch, A.B., Trombetta, F., Braga, A.C.M., Boeira, S.P., Oliveira, M.S., Dilkin, P., Mallmann, C.A., Fighera, M.R., Royes, L.F.F., Oliveira, M.S. and Furian, A.F., 2014. Involvement of oxidative stress in subacute toxicity induced by fumonisin B1 in broiler chicks. Veterinary Microbiology 174: 180-185.
'Involvement of oxidative stress in subacute toxicity induced by fumonisin B1 in broiler chicks ' () 174 Veterinary Microbiology : 180 -185.
-
Riley, R.T., Wang, E., Schroeder, J.J., Smit, E.R., Plattner, R.D., Abbas, H., Yoo, H.S. and Merrill Jr., A.H., 1996. Evidence for disruption of sphingolipid metabolism as a contributing factor in the toxicity and carcinogenicity of fumonisins. Natural Toxins: 4: 3-15.
'Evidence for disruption of sphingolipid metabolism as a contributing factor in the toxicity and carcinogenicity of fumonisins ' () 4 Natural Toxins: : 3 -15.
-
Schatzmayr, G. and Streit, E., 2013. Global occurrence of mycotoxins in the food and feed chain: facts and figures. World Mycotoxin Journal 6: 213-222.
'Global occurrence of mycotoxins in the food and feed chain: facts and figures ' () 6 World Mycotoxin Journal : 213 -222.
-
Schlatter, J., 2004. Toxicity data relevant for hazard characterization. Toxicology Letters 153: 83-89.
'Toxicity data relevant for hazard characterization ' () 153 Toxicology Letters : 83 -89.
-
Schuhmacher-Wolz, U., Heine, K. and Schneider, K., 2010. Report on toxicity data on trichothecene mycotoxins HT-2 and T-2 toxins. Available at:http://www.efsa.europa.eu/en/scdocs/doc/65e.pdf.
-
Sedlak, I. and Lindsay, R.H., 1968. Estimation of total, protein-bound and nonprotein sulfhydryl groups in tissues with Ellmannâs reagent. Analytical Biochemistry 25: 192-205.
'Estimation of total, protein-bound and nonprotein sulfhydryl groups in tissues with Ellmannâs reagent ' () 25 Analytical Biochemistry : 192 -205.
-
Sokolovic, M., Garaj-Vrhovac, V., Ramic, S. and Simpraga, B., 2007. Chicken nucleated blood cells as a cellular model for genotoxicity testing using the comet assay. Food and Chemical Toxicology 45: 2165-2170.
'Chicken nucleated blood cells as a cellular model for genotoxicity testing using the comet assay ' () 45 Food and Chemical Toxicology : 2165 -2170.
-
Soriano, M.J. and Dragacci, S., 2004. Occurrence of fumonisins in food. Food Research International 37: 985-1000.
'Occurrence of fumonisins in food ' () 37 Food Research International : 985 -1000.
-
Speijers, G.J.A. and Speijers, M.H.M., 2004. Combined toxic effects of mycotoxins. Toxicology Letters 153: 91-98.
'Combined toxic effects of mycotoxins ' () 153 Toxicology Letters : 91 -98.
-
Streit, E., Schatzmayr, G., Tassis, P., Tzika, E., Marin, D., Taranu, I., Tabuc, C., Nicolau, A., Aprodu, I., Puel, O. and Oswald, I.P., 2012. Current situation of mycotoxin contamination and co-occurrence in animal feed-focus on Europe. Toxins 4: 788-809.
'Current situation of mycotoxin contamination and co-occurrence in animal feed-focus on Europe ' () 4 Toxins : 788 -809.
-
Szabó, A., Szabó-Fodor, J., Fébel, H., Romvári, R. and Kovács, M., 2014. Individual and combined haematotoxic effects of fumonisin B1 and T-2 mycotoxins in rabbits. Food and Chemical Toxicology 72: 257-264.
'Individual and combined haematotoxic effects of fumonisin B1 and T-2 mycotoxins in rabbits ' () 72 Food and Chemical Toxicology : 257 -264.
-
Szilágyi, M., Fekete, S., Huszenicza, G.Y. and Albert, M., 1994. Biochemical and physiological effects of long-term sublethal T-2 toxin feeding in rabbits. Acta Biologica Hungarica 45: 69-76.
'Biochemical and physiological effects of long-term sublethal T-2 toxin feeding in rabbits ' () 45 Acta Biologica Hungarica : 69 -76.
-
Temesváry, K. and Oppel, K., 2001. Metabolism of glycated proteins in the rabbit. 2. The serum/plasma fructosamine and blood plasma glucose levels in rabbits around kindling (in Hungarian). Magyar Ãllatorvosok Lapa 123: 297-301.
'Metabolism of glycated proteins in the rabbit ' () 123 Magyar Ãllatorvosok Lapa : 297 -301.
-
Van der Fels-Klerx, H.J. and Stratakou, I., 2010. T-2 toxin and HT-2 toxin in grain and grain-based commodities in Europe: occurrence, factors affecting occurrence, co-occurrence and toxicological effects. World Mycotoxin Journal 3: 349-367.
'T-2 toxin and HT-2 toxin in grain and grain-based commodities in Europe: occurrence, factors affecting occurrence, co-occurrence and toxicological effects ' () 3 World Mycotoxin Journal : 349 -367.
-
Wang, J.S. and Groopman, J.D., 1999. DNA damage by mycotoxins. Mutation Research 424: 167-181.
'DNA damage by mycotoxins ' () 424 Mutation Research : 167 -181.
-
Waskiewicz, A., Beszterda, M. and Golinski, P., 2012. Occurrence of fumonisins in food â an interdisciplinary approach to the problem. Food Control 26: 491-499.
'Occurrence of fumonisins in food â an interdisciplinary approach to the problem ' () 26 Food Control : 491 -499.
| å ¨é¨æé´ | è¿å»ä¸å¹´ | è¿å»30天 | |
|---|---|---|---|
| æè¦æµè§æ¬¡æ° | 223 | 104 | 7 |
| å ¨ææµè§æ¬¡æ° | 23 | 1 | 0 |
| PDFä¸è½½æ¬¡æ° | 6 | 0 | 0 |
Individual and combined effects of feed artificially contaminated with with fumonisin B1 and T-2 toxin in weaned rabbits
äºWorld Mycotoxin JournalSearch for other papers by D. Hafner in
Current site
Google Scholar
PubMed
Search for other papers by A. Szabó in
Current site
Google Scholar
PubMed
Search for other papers by L. DâCosta in
Current site
Google Scholar
PubMed
Search for other papers by J. Szabó-Fodor in
Current site
Google Scholar
PubMed
Search for other papers by G. Tornyos in
Current site
Google Scholar
PubMed
Search for other papers by Zs. Blochné Bodnár in
Current site
Google Scholar
PubMed
Search for other papers by K. Ãlbeiné Horvatovich in
Current site
Google Scholar
PubMed
Search for other papers by E. Baloghné Zándoki in
Current site
Google Scholar
PubMed
Search for other papers by B. Bóta in
Current site
Google Scholar
PubMed
MTA-KE Mycotoxins in the Food Chain Research Group, Faculty of Agricultural and Environmental Sciences, Kaposvár University, Guba S. 40., Kaposvár 7400, Hungary.
Search for other papers by M. Kovács in
Current site
Google Scholar
PubMed
Co-contamination of feed and feed raw materials with two or more mycotoxins is frequently reported, however, only a few studies have investigated the combined effects of low doses of multiple mycotoxins. In the present study the individual and combined effects of 10 mg/kg fumonisin B1 and 2 mg/kg T-2 toxin (n=12/group) were investigated in weaned rabbits. Mycotoxin contaminated feed was produced by adding fungal cultures ofFusarium verticillioides andFusarium sporotrichioides, and fed to 40 days old rabbits during 28 days. Feed intake and body weight were measured weekly, serum biochemistry and antioxidant parameters on day 0, 14 and 28, while histopathological examination and comet assay were performed at the end of the experiment. T-2 exposure both alone and in combination resulted in 15-18% less final body weight compared to the control and FB1 treatment. There was a significant increase in the concentration of plasma total protein, albumin, fructosamine and creatinine in the group treated with FB1 compared to the control. The liver and the kidney of most animals treated with T-2 toxin, FB1 and their combination showed pathological changes, occurring more frequent in animals exposed to both toxins. T-2 resulted in depletion of lymphocytes in the spleen. FB1 and T-2 exerted synergistic effect on the antioxidant/oxidative parameters after 2 weeks of exposure, manifesting in less glutathione and glutathione peroxidase, while more malondialdehyde was produced. Both toxins caused DNA damage in the lymphocytes, which was more pronounced in the group fed T-2 toxin and T-2 combined with FB1, without additive or synergistic effects.
| å ¨é¨æé´ | è¿å»ä¸å¹´ | è¿å»30天 | |
|---|---|---|---|
| æè¦æµè§æ¬¡æ° | 223 | 104 | 7 |
| å ¨ææµè§æ¬¡æ° | 23 | 1 | 0 |
| PDFä¸è½½æ¬¡æ° | 6 | 0 | 0 |
