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An analytical method for quantifying zearalenone (ZEA) in pet foods using a liquid chromatograph-atmospheric pressure chemical ionization-mass spectrometer (LC-APCI-MS) was developed, and an inter-laboratory study was conducted. ZEA was extracted from pet food samples with aqueous acetonitrile. The extract was purified using a multifunctional column, MultiSep 226 Aflazon+, and analysed with LC-APCI-MS. The limit of quantification of ZEA by this method was estimated to be 0.2 mg/kg for dry and semi-dry pet foods, and 0.1 mg/kg for wet pet food. The calibration curve of ZEA for this method showed linearity in the range of 0.1-10 ng of ZEA (0.01-1.0 µg/ml, 0.04-4.0 mg/kg matrix). The mean recoveries, repeatabilities, reproducibilities, and the HorRats for ZEA from the inter-laboratory validation study were 99.0-102%, 2.2-3.0%, 5.6-6.6%, and 0.33-0.36, respectively.
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AOAC International, 2012. Interlaboratory collaborative study, Appendix D Guidelines for collaborative study procedures to validate characteristics of a method of analysis: 5.2 Outliers. Official Methods of Analysis, 19th ed., AOAC International, Gaithersburg, MD, USA.
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Mycotoxins and the pet food industry International Journal of Microbiology 119 95 102
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Survey and risk assessment of the mycotoxins deoxynivalenol, zearalenone, fumonisins, ochratoxin A, and aflatoxins in commercial dry dog food Mycotoxin Research 26 147 153
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Determination of zearalenone in cereal grains, animal feed, and feed ingredients using immunoaffinity column chromatography and liquid chromatography: interlaboratory study Journal of AOAC International 90 1610 1622
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Commission Recommendation of 17 August 2006 on the presence of deoxynivalenol, zearalenone, ochratoxin A, T-2, and HT-2 and fumonisins in products intended for animal feeding Official Journal of the European Union 229 7 9
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Scientific Opinion on the risks for public health related to presence of zearalenone in food EFSA Journal 9 2197
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Determination of zearalenone content in cereals and feedstuffs by immunoaffinity column coupled with lipid chromatography Journal of AOAC International 84 1453 1459
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Histopathological examination of ovaries in bitches after experimental zearalenone mycotoxicosis Polish Journal of Veterinary Sciences 11 363 366
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The characteristics of the chosen mycotoxins and their toxic influence on the human and animal metabolism Natural Science 2 544 550
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Differential estrogenic actions of endocrine-disrupting chemicals bisphenol A, bisphenol AF, and zearalenone through estrogen receptor α and βin vitro Environmental Health Perspectives 120 1029 1035
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A survey of the occurrence ofFusarium mycotoxins in biscuits in Japan by using LC/MS Journal of Health Science 56 188 194
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Review on the toxicity, occurrence, metabolism, detoxification, regulation and intake of zearalenone: an oestrogenic mycotoxin Food and Chemical Toxicology 45 1 18
| Insgesamt | Letzte 365 Tage | In den letzten 30 Tagen | |
|---|---|---|---|
| Aufrufe von Kurzbeschreibungen | 123 | 46 | 0 |
| Gesamttextansichten | 26 | 1 | 0 |
| PDF-Downloads | 3 | 0 | 0 |
An analytical method for quantifying zearalenone (ZEA) in pet foods using a liquid chromatograph-atmospheric pressure chemical ionization-mass spectrometer (LC-APCI-MS) was developed, and an inter-laboratory study was conducted. ZEA was extracted from pet food samples with aqueous acetonitrile. The extract was purified using a multifunctional column, MultiSep 226 Aflazon+, and analysed with LC-APCI-MS. The limit of quantification of ZEA by this method was estimated to be 0.2 mg/kg for dry and semi-dry pet foods, and 0.1 mg/kg for wet pet food. The calibration curve of ZEA for this method showed linearity in the range of 0.1-10 ng of ZEA (0.01-1.0 µg/ml, 0.04-4.0 mg/kg matrix). The mean recoveries, repeatabilities, reproducibilities, and the HorRats for ZEA from the inter-laboratory validation study were 99.0-102%, 2.2-3.0%, 5.6-6.6%, and 0.33-0.36, respectively.
| Insgesamt | Letzte 365 Tage | In den letzten 30 Tagen | |
|---|---|---|---|
| Aufrufe von Kurzbeschreibungen | 123 | 46 | 0 |
| Gesamttextansichten | 26 | 1 | 0 |
| PDF-Downloads | 3 | 0 | 0 |