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Development and inter-laboratory study of a method for quantifying zearalenone in pet foods

in World Mycotoxin Journal
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M. Nomura Food and Agricultural Materials Inspection Center, Fertilizer and Feed Inspection Department, Saitama Shintoshin National Government Building, Kensato Building, Shintoshin 2-1, Chuo-ku, Saitama-shi, Saitama 330-9731, Japan.

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T. Wada Chemicals Evaluation and Research, Institute, Japan (CERI), Tokyo, Environmental Technology Department, 1600 Shimotakano, Sugito-machi, Kitakatsushika-gun, Saitama 345-0043, Japan.

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T. Ishibashi Food and Agricultural Materials Inspection Center, Fertilizer and Feed Inspection Department, Saitama Shintoshin National Government Building, Kensato Building, Shintoshin 2-1, Chuo-ku, Saitama-shi, Saitama 330-9731, Japan.

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T. Komoriya Food and Agricultural Materials Inspection Center, Fertilizer and Feed Inspection Department, Saitama Shintoshin National Government Building, Kensato Building, Shintoshin 2-1, Chuo-ku, Saitama-shi, Saitama 330-9731, Japan.

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T. Nagahara Food and Agricultural Materials Inspection Center, Fertilizer and Feed Inspection Department, Saitama Shintoshin National Government Building, Kensato Building, Shintoshin 2-1, Chuo-ku, Saitama-shi, Saitama 330-9731, Japan.

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T. Chihara Food and Agricultural Materials Inspection Center, Fertilizer and Feed Inspection Department, Saitama Shintoshin National Government Building, Kensato Building, Shintoshin 2-1, Chuo-ku, Saitama-shi, Saitama 330-9731, Japan.

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An analytical method for quantifying zearalenone (ZEA) in pet foods using a liquid chromatograph-atmospheric pressure chemical ionization-mass spectrometer (LC-APCI-MS) was developed, and an inter-laboratory study was conducted. ZEA was extracted from pet food samples with aqueous acetonitrile. The extract was purified using a multifunctional column, MultiSep 226 Aflazon+, and analysed with LC-APCI-MS. The limit of quantification of ZEA by this method was estimated to be 0.2 mg/kg for dry and semi-dry pet foods, and 0.1 mg/kg for wet pet food. The calibration curve of ZEA for this method showed linearity in the range of 0.1-10 ng of ZEA (0.01-1.0 µg/ml, 0.04-4.0 mg/kg matrix). The mean recoveries, repeatabilities, reproducibilities, and the HorRats for ZEA from the inter-laboratory validation study were 99.0-102%, 2.2-3.0%, 5.6-6.6%, and 0.33-0.36, respectively.

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