A new preparative method for simultaneous purification of ochratoxin A and ochratoxin B from wheat culture inoculated withAspergillus ochraceus
äºWorld Mycotoxin JournalInstitute for Agro-food Standards and Testing Technology, Laboratory of Quality & Safety Risk Assessment for Agro-products (Shanghai), Shanghai Academy of Agricultural Sciences, 1000 Jinqi Road, 201403 Shanghai, China P.R.
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Ochratoxins are a mycotoxin family frequently found in agricultural commodities worldwide and pose a potential health risk to humans and animals. To obtain large amounts of high-purity ochratoxins for food safety monitoring and toxicological research, a novel and effective method was established for simultaneous purification of ochratoxin A (OTA) and ochratoxin B (OTB) from a wheat culture inoculated with an ochratoxin-producingAspergillus strain. The inoculated wheat culture was first extracted with methanol:water (80:20, v/v), followed by one or two cleanup procedures involving acid-assisted liquid-liquid extraction and gel permeation chromatography. Subsequently, target analytes were separated and collected using preparative high performance liquid chromatography. Finally, a combined approach of ultra-high performance liquid chromatography, ultraviolet spectrophotometry and mass spectrometry was applied for purity analysis and structural identification of the obtained toxins. As a result, 100 g of an inoculated wheat culture yielded 69 mg of OTA and 6 mg of OTB with purities greater than 98%. This proposed method might serve as a valuable reference to obtain expensive ochratoxin standards. To the best of our knowledge, this is the first report on simultaneous preparation of OTA and OTB from artificially inoculated wheat culture.
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Supplementary Materials
| å ¨é¨æé´ | è¿å»ä¸å¹´ | è¿å»30天 | |
|---|---|---|---|
| æè¦æµè§æ¬¡æ° | 413 | 98 | 22 |
| å ¨ææµè§æ¬¡æ° | 32 | 1 | 0 |
| PDFä¸è½½æ¬¡æ° | 11 | 4 | 0 |
A new preparative method for simultaneous purification of ochratoxin A and ochratoxin B from wheat culture inoculated withAspergillus ochraceus
äºWorld Mycotoxin JournalInstitute for Agro-food Standards and Testing Technology, Laboratory of Quality & Safety Risk Assessment for Agro-products (Shanghai), Shanghai Academy of Agricultural Sciences, 1000 Jinqi Road, 201403 Shanghai, China P.R.
Search for other papers by Z.Y. Zhao in
Current site
Google Scholar
PubMed
Search for other papers by N. Liu in
Current site
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PubMed
Search for other papers by L.C. Yang in
Current site
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PubMed
Search for other papers by A.B. Wu in
Current site
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PubMed
Search for other papers by Z.L. Zhou in
Current site
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PubMed
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PubMed
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PubMed
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PubMed
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PubMed
Ochratoxins are a mycotoxin family frequently found in agricultural commodities worldwide and pose a potential health risk to humans and animals. To obtain large amounts of high-purity ochratoxins for food safety monitoring and toxicological research, a novel and effective method was established for simultaneous purification of ochratoxin A (OTA) and ochratoxin B (OTB) from a wheat culture inoculated with an ochratoxin-producingAspergillus strain. The inoculated wheat culture was first extracted with methanol:water (80:20, v/v), followed by one or two cleanup procedures involving acid-assisted liquid-liquid extraction and gel permeation chromatography. Subsequently, target analytes were separated and collected using preparative high performance liquid chromatography. Finally, a combined approach of ultra-high performance liquid chromatography, ultraviolet spectrophotometry and mass spectrometry was applied for purity analysis and structural identification of the obtained toxins. As a result, 100 g of an inoculated wheat culture yielded 69 mg of OTA and 6 mg of OTB with purities greater than 98%. This proposed method might serve as a valuable reference to obtain expensive ochratoxin standards. To the best of our knowledge, this is the first report on simultaneous preparation of OTA and OTB from artificially inoculated wheat culture.
| å ¨é¨æé´ | è¿å»ä¸å¹´ | è¿å»30天 | |
|---|---|---|---|
| æè¦æµè§æ¬¡æ° | 413 | 98 | 22 |
| å ¨ææµè§æ¬¡æ° | 32 | 1 | 0 |
| PDFä¸è½½æ¬¡æ° | 11 | 4 | 0 |
