Aspergillus fumigatus is a ubiquitous airborne pathogen. Saprophytic growth in the presence of environmental mycotoxins might affect its fitness and virulence. T-2 toxin (T-2) is a trichothecene mycotoxin produced byFusarium spp. in various substrates. This study aimed to evaluate the effects of T-2 on the fitness ofA. fumigatus in vitro and its virulence in experimentally inoculated chickens. We culturedA. fumigatus on agar media containing T-2, and examined the changes in viability, morphology, growth rate, proteome expression, and susceptibility to antimycotics and oxidative stress of this fungus. Results showed that exposure to 1000 ng/ml T-2 in the substrate did not reduce the viability ofA. fumigatus, but its growth was inhibited, with wrinkling and depigmentation of the colonies. Proteomic analysis revealed 21 upregulated proteins and 33 downregulated proteins, including those involved in stress response, pathogenesis, metabolism, transcription. The proteome seems to have shifted to enhance the glycolysis, catabolism of lipids, and amino acid conversion. Assays on fungal susceptibility to antimycotics and oxidative stress showed that T-2 exposure did not affect the minimal inhibitory concentrations of amphotericin B, itraconazole, voriconazole and terbinafine againstA. fumigatus, but increased the susceptibility ofA. fumigatus to H2O2 and menadione. Experimental inoculation of chickens withA. fumigatus showed that exposure ofA. fumigatus to T-2 significantly exacerbated aspergillosis in chickens exposed to dietary T-2. In conclusion,A. fumigatus is capable of surviving and growing on substrates containing levels of T-2 up to 1000 ng/ml. Growth in presence of T-2 induces a stress response inA. fumigatus, which is associated with exacerbation of aspergillosisin vivo.
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Asif, A.R., Oellerich, M., Amstrong, V.W., Riemenschneider, B., Monod, M. and Reichard, U., 2006. Proteome of conidial surface associated proteins ofAspergillus fumigatus reflecting potential vaccine candidates and allergens. Journal of Proteome Research 5: 954-962.
'Proteome of conidial surface associated proteins of Aspergillus fumigatus reflecting potential vaccine candidates and allergens ' () 5 Journal of Proteome Research : 954 -962.
Beernaert, L.A., Pasmans, F., Haesebrouck, F. and Martel, A., 2008. ModellingAspergillus fumigatus infections in racing pigeons (Columba livia domestica). Avian Pathology 37: 545-549.
'Modelling Aspergillus fumigatus infections in racing pigeons (Columba livia domestica) ' () 37 Avian Pathology : 545 -549.
Beernaert, L.A., Pasmans, F., Van Waeyenberghe, L., Dorrestein, G.M., Verstappen, F., Vercammen, F., Haesebrouck, F. and Martel, A., 2009. AvianAspergillus fumigatus strains resistant to both itraconazole and voriconazole. Antimicrobial Agents and Chemotherapy 53: 2199-2201.
'Avian Aspergillus fumigatus strains resistant to both itraconazole and voriconazole ' () 53 Antimicrobial Agents and Chemotherapy : 2199 -2201.
Beernaert, L.A., Pasmans, F., Van Waeyenberghe, L., Haesebrouck, F. and Martel, A., 2010.Aspergillus infections in birds: a review. Avian Pathology 39: 325-331.
'Aspergillus infections in birds: a review ' () 39 Avian Pathology : 325 -331.
Bijttebier, J., Tilleman, K., Deforce, D., Dhaenens, M., Van Soom, A. and Maes, D., 2009. Proteomic study to identify factors in follicular fluid and/or serum involved inin vitro cumulus expansion of porcine oocytes. Society of Reproduction and Fertility Supplement 66: 205-206.
'Proteomic study to identify factors in follicular fluid and/or serum involved in in vitro cumulus expansion of porcine oocytes ' () 66 Society of Reproduction and Fertility Supplement : 205 -206.
Binder, E.M., Tan, L.M., Chin, L.J., Handl, J. and Richard, J., 2007. Worldwide occurrence of mycotoxins in commodities, feeds and feed ingredients. Animal Feed Science and Technology 137: 265-282.
'Worldwide occurrence of mycotoxins in commodities, feeds and feed ingredients ' () 137 Animal Feed Science and Technology : 265 -282.
Branco, M.R., Marinho, H.S., Cyrne, L. and Antunes, F., 2004. Decrease of H2O2 plasma membrane permeability during adaptation to H2O2 inSaccharomyces cerevisiae. Journal of Biological Chemistry 279: 6501-6506.
'Decrease of H2O2 plasma membrane permeability during adaptation to H2O2 in Saccharomyces cerevisiae ' () 279 Journal of Biological Chemistry : 6501 -6506.
Burmeister, H.R. and Hesseltine, C.W., 1970. Biological assays of two mycotoxins produced byFusarium tricinctum. Applied and Environmental Microbiology 20: 437-440.
'Biological assays of two mycotoxins produced by Fusarium tricinctum ' () 20 Applied and Environmental Microbiology : 437 -440.
Colaert, N., Van Huele, C., Degroeve, S., Staes, A., Vandekerckhove, J., Gevaert, K. and Martens, L., 2011. Combining quantitative proteomics data processing workflows for greater sensitivity. Nature Methods 8: 481-483.
'Combining quantitative proteomics data processing workflows for greater sensitivity ' () 8 Nature Methods : 481 -483.
Corriera, D.E. and Ziprin, R.L., 1987. Immunotoxic effects of T-2 mycotoxin on cell-mediated resistance toListeria monocytogenes infection. Veterinary Immunology and Immunopathology 14: 11-21.
'Immunotoxic effects of T-2 mycotoxin on cell-mediated resistance to Listeria monocytogenes infection ' () 14 Veterinary Immunology and Immunopathology : 11 -21.
Dannaoui, E., Meletiadis, J., Tortorano, A.M., Symoens, F., Nolard, N., Viviani, M.A., Piens, M.A., Lebeau, B., Verweij, P.E., Grillot, R. and the EBGA Network, 2004. Susceptibility testing of sequential isolates ofAspergillus fumigatus recovered from treated patients. Journal of Medical Microbiology 53: 129-134.
'Susceptibility testing of sequential isolates of Aspergillus fumigatus recovered from treated patients ' () 53 Journal of Medical Microbiology : 129 -134.
Elmholt, S., 2008. Mycotoxins in the soil environment. In: Karlovsky, P. (ed.) Secondary metabolites in soil ecology, soil biology. Springer, Berlin, Germany, pp. 167-203.
Secondary metabolites in soil ecology, soil biology , () 167 -203.
European Food Safety Authority (EFSA), 2011. Scientific opinion on the risks for animal and public health related to the presence of T-2 and HT-2 toxin in food and feed. EFSA Journal 9: 2481-2668.
Gautam, P., Shankar, J., Madan, T., Sirdeshmukh, R., Sundaram, C.S., Gade, W.N., Basir, S.F. and Sarma, P.U., 2008. Proteomic and transcriptomic analysis ofAspergillus fumigatus on exposure to amphotericin B. Antimicrobial Agents and Chemotherapy 52: 4220-4227.
'Proteomic and transcriptomic analysis of Aspergillus fumigatus on exposure to amphotericin B ' () 52 Antimicrobial Agents and Chemotherapy : 4220 -4227.
Hoerger, C.C., Schenzel, J., Strobel, B.W. and Bucheli, T.D., 2009. Analysis of selected phytotoxins and mycotoxins in environmental samples. Analytical and Bioanalytical Chemistry 395: 1261-1289.
'Analysis of selected phytotoxins and mycotoxins in environmental samples ' () 395 Analytical and Bioanalytical Chemistry : 1261 -1289.
Johnson, L.B. and Kauffman, C.A., 2003. Voriconazole: a new triazole antifungal agent. Clinical Infectious Diseases 36: 630-637.
'Voriconazole: a new triazole antifungal agent ' () 36 Clinical Infectious Diseases : 630 -637.
Juul-Madsen, H.R., Viertlboeck, B., Smith, A.L. and Göbel, T.W.F., 2008. Avian innate immune responses. In: Davison, F., Kaspers, B. and Schat, K.A. (eds.) Avian immunology. Elsevier, San Diego, CA, USA, pp.129-158.
Avian immunology , () 129 -158.
Kubena, L.F., Bailey, R.H., Byrd, J.A., Young, C.R., Corrier, D.E., Stanker, L.H. and Rottinghaust, G.E., 2001. Cecal volatile fatty acids and broiler chick susceptibility toSalmonella Typhimurium colonization as affected by aflatoxins and T-2 toxin. Poultry Science 80: 411-417.
'Cecal volatile fatty acids and broiler chick susceptibility to Salmonella Typhimurium colonization as affected by aflatoxins and T-2 toxin ' () 80 Poultry Science : 411 -417.
Latgé, J.P., 1999.Aspergillus fumigatus and aspergillosis. Clinical Microbiology Reviews 12: 310-350.
'Aspergillus fumigatus and aspergillosis ' () 12 Clinical Microbiology Reviews : 310 -350.
Li, S.J., Njumbe Ediage, E., De Saeger, S., Van Waeyenberghe, L., Garmyn, A., Verlinden, M., Ducatelle, R., Croubels, S., Haesebrouck, F., Pasmans, F. and Martel, A., 2013a. Occurrence and pathology of mycotoxins in commercial parrot feeds. World Mycotoxin Journal 6: 449-453.
'Occurrence and pathology of mycotoxins in commercial parrot feeds ' () 6 World Mycotoxin Journal : 449 -453.
Li, S.J., Pasmans, F., Croubels, S., Verbrugghe, E., Van Waeyenberghe, L., Yang, Z., Haesebrouck, F. and Martel, A., 2013b. T-2 toxin impairs antifungal activities of chicken macrophages againstAspergillus fumigatus conidia but promotes the pro-inflammatory responses. Avian Pathology 42: 457-463.
'T-2 toxin impairs antifungal activities of chicken macrophages against Aspergillus fumigatus conidia but promotes the pro-inflammatory responses ' () 42 Avian Pathology : 457 -463.
Li, Z. and Srivastava, P., 2004. Heat-shock proteins. Current protocols in immunology, appendix 1. DOI: http://dx.doi.org/10.1002/0471142735.ima01ts58.
Madhyastha, M.S., Marquardt, R.R., Masi, A., Borsa, J. and Frohlich, A.A., 1994. Comparison of toxicity of different mycotoxins to several species of bacteria and yeasts: use ofBacillus brevis in a disc diffusion assay. Journal of Food Protection 57: 48-53.
'Comparison of toxicity of different mycotoxins to several species of bacteria and yeasts: use of Bacillus brevis in a disc diffusion assay ' () 57 Journal of Food Protection : 48 -53.
Mayer, S., Engelhart, S., Kolk, A. and Blome, H., 2008. The significance of mycotoxins in the framework of assessing workplace related risks. Mycotoxin Research 24: 151-164.
'The significance of mycotoxins in the framework of assessing workplace related risks ' () 24 Mycotoxin Research : 151 -164.
McMillan, M.C. and Petrak, M.L., 1989. Retrospective study of aspergillosis in pet birds. Journal of the Association of Avian Veterinarians 3: 211-215.
'Retrospective study of aspergillosis in pet birds ' () 3 Journal of the Association of Avian Veterinarians : 211 -215.
Moore, C.B., Walls, C.M. and Denning, D.W., 2001.In vitro activities of terbinafine againstAspergillus species in comparison with those of itraconazole and amphotericin B. Antimicrobial Agents and Chemotherapy 45: 1882-1885.
'In vitro activities of terbinafine against Aspergillus species in comparison with those of itraconazole and amphotericin B ' () 45 Antimicrobial Agents and Chemotherapy : 1882 -1885.
Nordby, K.C., Halstensen, A.S., Elen, O., Clasen, P.E., Langseth, W., Kristensen, P. and Eduard, W., 2004. Trichothecene mycotoxins and their determinants in settled dust related to grain production. Annals of Agricultural and Environmental Medicine 11: 75-83.
'Trichothecene mycotoxins and their determinants in settled dust related to grain production ' () 11 Annals of Agricultural and Environmental Medicine : 75 -83.
Osselaere, A., Li, S.J., De Bock, L., Devreese, M., Goossens, J., Vandenbroucke, V., Van Bocxlaer, J., Boussery, K., Pasmans, F., Martel, A., De Backer, P. and Croubels, S., 2013. Toxic effects of dietary exposure to T-2 toxin on intestinal and hepatic biotransformation enzymes and drug transporter systems in broiler chickens. Food and Chemical Toxicology 55: 150-155.
'Toxic effects of dietary exposure to T-2 toxin on intestinal and hepatic biotransformation enzymes and drug transporter systems in broiler chickens ' () 55 Food and Chemical Toxicology : 150 -155.
Pócsi, I., Miskei, M., Karányi, Z., Emri, T., Ayoubi, P., Pusztahelyi, T., Balla, G. and Prade, R.A., 2005. Comparison of gene expression signatures of diamide, H2O2 and menadione exposedAspergillus nidulans cultures - linking genome-wide transcriptional changes to cellular physiology. BMC Genomics 6: 182.
'Comparison of gene expression signatures of diamide, H2O2 and menadione exposed Aspergillus nidulans cultures - linking genome-wide transcriptional changes to cellular physiology ' () 6 BMC Genomics : 182.
Qiao, J., Liu, W. and Li, R., 2010. Truncated Afyap1 attenuates antifungal susceptibility ofAspergillus fumigatus to voriconazole and confers adaptation of the fungus to oxidative stress. Mycopathologia 170: 155-160.
'Truncated Afyap1 attenuates antifungal susceptibility of Aspergillus fumigatus to voriconazole and confers adaptation of the fungus to oxidative stress ' () 170 Mycopathologia : 155 -160.
Samson, R.A., Houbraken, J.A.M.P., Kuijpers, A.F.A., Frank, J.M. and Frisvad, J.C., 2004. New ochratoxin A or sclerotium producing species inAspergillus sectionNigri. Studies in Mycology 50: 45-61.
'New ochratoxin A or sclerotium producing species in Aspergillus section Nigri ' () 50 Studies in Mycology : 45 -61.
Shifrin, V.I. and Anderson, P., 1999. Trichothecene mycotoxins trigger a ribotoxic stress response that activates c-Jun N-terminal kinase and p38 mitogen-activated protein kinase and induces apoptosis. Journal of Biological Chemistry 274: 13985-13992.
'Trichothecene mycotoxins trigger a ribotoxic stress response that activates c-Jun N-terminal kinase and p38 mitogen-activated protein kinase and induces apoptosis ' () 274 Journal of Biological Chemistry : 13985 -13992.
Smoragiewicz, W., Cossette, B., Boutard, A. and Krzystyniak, K., 1993. Trichothecene mycotoxins in the dust of ventilation systems in office buildings. International Archives of Occupational and Environmental Health 65: 113-117.
'Trichothecene mycotoxins in the dust of ventilation systems in office buildings ' () 65 International Archives of Occupational and Environmental Health : 113 -117.
Sokolovic, M., Garaj-Vrhovac, V. and Simpraga, B., 2008. T-2 toxin: incidence and toxicity in poultry. Archives of Industrial Hygiene and Toxicology 59: 43-52.
'T-2 toxin: incidence and toxicity in poultry ' () 59 Archives of Industrial Hygiene and Toxicology : 43 -52.
Sugareva, V., Härtl, A., Brock, M., Hübner, K., Rohde, M., Heinekamp, T. and Brakhage, A.A., 2006. Characterisation of the laccase-encoding geneabr2 of the dihydroxynaphthalene-like melanin gene cluster ofAspergillus fumigatus. Archives of Microbiology 186: 345-355.
'Characterisation of the laccase-encoding gene abr2 of the dihydroxynaphthalene-like melanin gene cluster of Aspergillus fumigatus ' () 186 Archives of Microbiology : 345 -355.
Sun, J.S., Tsuang, Y.H., Huang, W.C., Chen, L.T., Hang, Y.S. and Lu, F.J., 1997. Menadione-induced cytotoxicity to rat osteoblasts. Cellular and Molecular Life Sciences 53: 967-976.
'Menadione-induced cytotoxicity to rat osteoblasts ' () 53 Cellular and Molecular Life Sciences : 967 -976.
Toledano, M.B., Delaunay, A., Biteau, B., Spector, D. and Azevedo, D., 2003. Oxidative stress responses in yeast. In: Hohman, S. and Mager, W.H. (eds.) Yeast stress responses. Springer, Berlin, Germany, pp. 241-304.
Yeast stress responses , () 241 -304.
Tuomi, T., Reijula, K., Johnsson, T., Hemminki, K., Hintikka, E.L., Lindroos, O., Kalso, S., Koukila-Kähkölä, P., Mussalo-Rauhamaa, H. and Haahtela, T., 2000. Mycotoxins in crude building materials from water-damaged buildings. Applied and Environmental Microbiology 66: 1899-1904.
'Mycotoxins in crude building materials from water-damaged buildings ' () 66 Applied and Environmental Microbiology : 1899 -1904.
Van Waeyenberghe, L., Fischer, D., Coenye, T., Ducatelle, R., Haesebrouck, F., Pasmans, F., Lierz, M. and Martel, A., 2012b. Susceptibility of adult pigeons and hybrid falcons to experimental aspergillosis. Avian Pathology 41: 563-567.
'Susceptibility of adult pigeons and hybrid falcons to experimental aspergillosis ' () 41 Avian Pathology : 563 -567.
Van Waeyenberghe, L., Pasmans, F., DâHerde, K., Ducatelle, R., Favoreel, H., Li, S.J., Haesebrouck, F. and Martel, A., 2012a. Germination ofAspergillus fumigatus inside avian respiratory macrophages is associated with cytotoxicity. Veterinary Research 43: 32.
'Germination of Aspergillus fumigatus inside avian respiratory macrophages is associated with cytotoxicity ' () 43 Veterinary Research : 32.
Vanderheyden, N., 1993. Aspergillosis in psittacine chicks. In: Jackson, G. (ed.). Proceedings of the Annual Conference of the Association of Avian Veterinarians, Nashville, TN, USA, pp. 207-212.
, , .
Verbrugghe, E., Vandenbroucke, V., Dhaenens, M., Shearer, N., Goossens, J., De Saeger, S., Eeckhout, M., DâHerde, K., Thompson, A., Deforce, D., Boyen, F., Leyman, B., Van Parys, A., De Backer, P., Haesebrouck, F., Croubels, S. and Pasmans, F., 2012. T-2 toxin inducedSalmonella Typhimurium intoxication results in decreasedSalmonella numbers in the cecum contents of pigs, despite marked effects onSalmonella-host cell interactions. Veterinary Research 43: 22.
'T-2 toxin induced Salmonella Typhimurium intoxication results in decreased Salmonella numbers in the cecum contents of pigs, despite marked effects on Salmonella-host cell interactions ' () 43 Veterinary Research : 22.
Vesonder, R.F., Ellis, J.J. and Rohwedder, W.K., 1981. Elaboration of vomitoxin and zearalenone byFusarium isolates and the biological activity ofFusarium-produced toxins. Applied and Environmental Microbiology 42: 1132-1134.
'Elaboration of vomitoxin and zearalenone by Fusarium isolates and the biological activity of Fusarium-produced toxins ' () 42 Applied and Environmental Microbiology : 1132 -1134.
Xu, H., Li, L.J., Wan, Z., Li, R.Y. and Liu, W., 2011. Dexamethasone increases susceptibility ofAspergillus fumigatus to hydrogen peroxide via down-regulation of Afyap1 gene expressionin vitro. Chinese Medical Journal 124: 3773-3777.
'Dexamethasone increases susceptibility of Aspergillus fumigatus to hydrogen peroxide via down-regulation of Afyap1 gene expression in vitro ' () 124 Chinese Medical Journal : 3773 -3777.
Ziprin, R.L. and Elissalde, M.H., 1990. Effect of T-2 toxin on resistance to systemicSalmonella Typhimurium infection of newly hatched chickens. American Journal of Veterinary Research 51: 1869-1872.
'Effect of T-2 toxin on resistance to systemic Salmonella Typhimurium infection of newly hatched chickens ' () 51 American Journal of Veterinary Research : 1869 -1872.
| å ¨é¨æé´ | è¿å»ä¸å¹´ | è¿å»30天 | |
|---|---|---|---|
| æè¦æµè§æ¬¡æ° | 307 | 126 | 15 |
| å ¨ææµè§æ¬¡æ° | 34 | 1 | 0 |
| PDFä¸è½½æ¬¡æ° | 12 | 0 | 0 |
Aspergillus fumigatus is a ubiquitous airborne pathogen. Saprophytic growth in the presence of environmental mycotoxins might affect its fitness and virulence. T-2 toxin (T-2) is a trichothecene mycotoxin produced byFusarium spp. in various substrates. This study aimed to evaluate the effects of T-2 on the fitness ofA. fumigatus in vitro and its virulence in experimentally inoculated chickens. We culturedA. fumigatus on agar media containing T-2, and examined the changes in viability, morphology, growth rate, proteome expression, and susceptibility to antimycotics and oxidative stress of this fungus. Results showed that exposure to 1000 ng/ml T-2 in the substrate did not reduce the viability ofA. fumigatus, but its growth was inhibited, with wrinkling and depigmentation of the colonies. Proteomic analysis revealed 21 upregulated proteins and 33 downregulated proteins, including those involved in stress response, pathogenesis, metabolism, transcription. The proteome seems to have shifted to enhance the glycolysis, catabolism of lipids, and amino acid conversion. Assays on fungal susceptibility to antimycotics and oxidative stress showed that T-2 exposure did not affect the minimal inhibitory concentrations of amphotericin B, itraconazole, voriconazole and terbinafine againstA. fumigatus, but increased the susceptibility ofA. fumigatus to H2O2 and menadione. Experimental inoculation of chickens withA. fumigatus showed that exposure ofA. fumigatus to T-2 significantly exacerbated aspergillosis in chickens exposed to dietary T-2. In conclusion,A. fumigatus is capable of surviving and growing on substrates containing levels of T-2 up to 1000 ng/ml. Growth in presence of T-2 induces a stress response inA. fumigatus, which is associated with exacerbation of aspergillosisin vivo.
| å ¨é¨æé´ | è¿å»ä¸å¹´ | è¿å»30天 | |
|---|---|---|---|
| æè¦æµè§æ¬¡æ° | 307 | 126 | 15 |
| å ¨ææµè§æ¬¡æ° | 34 | 1 | 0 |
| PDFä¸è½½æ¬¡æ° | 12 | 0 | 0 |