Heritability study of eGFP-transformedAspergillus flavus strains
äºWorld Mycotoxin JournalSearch for other papers by G.G. Moore in
Current site
Google Scholar
Field inoculation with non-aflatoxigenicAspergillus flavus is a preferred method for pre-harvest biocontrol of aflatoxin contamination of maize, cottonseed, and groundnut. Rationale for using theseA. flavus strains is that they (1) maintain persistent control of aflatoxigenic fungi in the field, and (2) are incapable of out-crossing. Trackable field-released biocontrol strains will be beneficial to study the movement and longevity of non-aflatoxigenicA. flavus strains. Incorporating a naturally-occurring compound such as enhanced green fluorescent protein (eGFP) into a biocontrol strain might allow observation of its behaviour in field settings. The success of long-term field testing of eGFP-expressingA. flavus strains depends on their ability to maintain fluorescence throughout growth. Additionally, to ensure accurate tracking of the fluorescent atoxigenic strain, the likelihood of their out-crossing with individuals from the native population must be determined.In vitro mating experiments paired each of six different eGFP-transformed atoxigenic strains with a highly fertile toxigenicA. flavus isolate. Findings indicate that the eGFP gene, and possibly the aflatoxin cluster, is heritable by the F1 progeny. Not all cultured ascospores were fluorescent, but subsequent growth arising from a single fluorescent ascospore exhibited fluorescence similar to the eGFP parent. Observed mixed-fluorescence among conidia in a single chain suggests heterokaryosis at the moment of conidiogenesis. Mycotoxin assays showed that some fluorescent F1 individuals produce aflatoxin and/or cyclopiazonic acid which would indicate they are recombinant offspring. The findings in this laboratory study lend support to concern that atoxigenic strains are not impervious to genetic recombination and for which, if possible in a natural environment, repeated use could pose a risk of increasing the occurrence of aflatoxigenic individuals in treated fields.
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Abbas, H.K., Zablotowicz, R.M. and Abel, C.A., 2006. Biocontrol of aflatoxin in corn by inoculation with non-aflatoxigenicAspergillus flavus isolates. Biocontrol Science and Technology 16: 437-449.
'Biocontrol of aflatoxin in corn by inoculation with non-aflatoxigenic Aspergillus flavus isolates ' () 16 Biocontrol Science and Technology : 437 -449.
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Carbone, I., Jakobek, J.L., Ramirez-Prado, J.H. and Horn, B.W., 2007. Recombination, balancing selection and adaptive evolution in the aflatoxin gene cluster ofAspergillus parasiticus. Molecular Ecology 16: 4401-4417.
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Chang, P.K., Horn, B.W. and Dorner, J.W., 2005. Sequence breakpoints in the aflatoxin biosynthesis gene cluster and flanking regions in nonaflatoxigenicAspergillus flavus isolates. Fungal Genetics and Biology 42: 914-923.
'Sequence breakpoints in the aflatoxin biosynthesis gene cluster and flanking regions in nonaflatoxigenic Aspergillus flavus isolates ' () 42 Fungal Genetics and Biology : 914 -923.
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Cotty, P.J., 1994. Influence of field application of an atoxigenic strain ofAspergillus flavus on the populations ofA. flavus infecting cotton bolls and on the aflatoxin content of cottonseed. Phytopathology 84: 1270-1277.
'Influence of field application of an atoxigenic strain of Aspergillus flavus on the populations of A. flavus infecting cotton bolls and on the aflatoxin content of cottonseed ' () 84 Phytopathology : 1270 -1277.
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Damann, K.E. and DeRobertis, C., 2013. Mating ofAspergillus flavus ÃAspergillus minisclerotigenes hybrids: are they functionally mules? Phytopathology 103: S2.32-S2.33.
'Mating of Aspergillus flavus à Aspergillus minisclerotigenes hybrids: are they functionally mules? ' () 103 Phytopathology : S2.32 -S2.33.
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Damann, K.E., DeRobertis, C. and Sweany, R.R., 2010. Mating betweenAspergillus flavus crytic species I and II. Phytopathology 100: S28.
'Mating between Aspergillus flavus crytic species I and II ' () 100 Phytopathology : S28.
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Dorner, J.W., 2009a. Biological control of aflatoxin contamination in corn using a nontoxigenic strain ofAspergillus flavus. Journal of Food Protection 72: 801-804.
'Biological control of aflatoxin contamination in corn using a nontoxigenic strain of Aspergillus flavus ' () 72 Journal of Food Protection : 801 -804.
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Dorner, J.W., 2009b. Development of biocontrol technology to manage aflatoxin contamination in peanuts. Peanut Science 36: 60-67.
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Doster, M.A., Morgan, D.P., Boeckler, L., Puckett, R., Cotty, P.J. and Michailides, T.J., 2012. Monitoring and improving the efficacy of the atoxigenic strain AF36 in commercial orchards and aflatoxin contamination of closed shell nuts. Annual Report. California Pistachio Industry, San Diego, CA, USA.
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Ehrlich, K.C. and Cotty, P.J., 2004. An isolate ofAspergillus flavus used to reduce aflatoxin contamination in cottonseed has a defective polyketide synthase gene. Applied Microbiology and Biotechnology 65: 473-478.
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Geiser, D.M., Pitt, J.I. and Taylor, J.W., 1998. Cryptic speciation and recombination in the aflatoxin-producing fungusAspergillus flavus. Proceedings of the National Academy of Sciences of the USA 95: 388-393.
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Horn, B.W., Moore, G.G. and Carbone, I., 2009b. Sexual production inAspergillus flavus. Mycologia 101: 423-429.
'Sexual production in Aspergillus flavus ' () 101 Mycologia : 423 -429.
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Horn, B.W., Moore, G.G. and Carbone, I., 2011. Sexual reproduction in aflatoxin-producingAspergillus nomius. Mycologia 103: 174-183.
'Sexual reproduction in aflatoxin-producing Aspergillus nomius ' () 103 Mycologia : 174 -183.
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Horn, B.W., Ramirez-Prado, J.H. and Carbone, I., 2009a. The sexual state ofAspergillus parasiticus. Mycologia 101: 275-280.
'The sexual state of Aspergillus parasiticus ' () 101 Mycologia : 275 -280.
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Horn, B.W., Ramirez-Prado, J.H. and Carbone, I., 2009c. Sexual reproduction and recombination in the aflatoxin-producing fungusAspergillus parasiticus. Fungal Genetics and Biology 46: 169-175.
'Sexual reproduction and recombination in the aflatoxin-producing fungus Aspergillus parasiticus ' () 46 Fungal Genetics and Biology : 169 -175.
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Horn, B.W., Sorensen, R., Lamb, M., Sobolev, V., Olarte, R., Worthington, C. and Carbone, I., 2013. Sexual reproduction inAspergillus flavus sclerotia naturally produced in corn. Phytopathology 104: 75-85.
'Sexual reproduction in Aspergillus flavus sclerotia naturally produced in corn ' () 104 Phytopathology : 75 -85.
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Huang, C., Jha, A., Sweany, R., DeRobertis, C. and Damann Jr., K.E., 2011. Intraspecific aflatoxin inhibition inAspergillus flavus is thigmoregulated, independent of vegetative compatibility group and is strain dependent. PLoS One 6: e23470.
'Intraspecific aflatoxin inhibition in Aspergillus flavus is thigmoregulated, independent of vegetative compatibility group and is strain dependent ' () 6 PLoS One : e23470.
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King, E.D., Bassi Jr., A.B., Ross, D.C. and Druebbisch, B., 2011. An industry perspective on the use of âatoxigenicâ strains ofAspergillus flavus as biological control agents and the significance of cyclopiazonic acid. Toxin Reviews 30: 33-41.
'An industry perspective on the use of âatoxigenicâ strains of Aspergillus flavus as biological control agents and the significance of cyclopiazonic acid ' () 30 Toxin Reviews : 33 -41.
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McAlpin, C.E. and Wicklow, D.T., 2005. Culture media and sources of nitrogen promoting the formation of stromata and ascocarps inPetromyces alliaceus (Aspergillus sectionFlavi). Canadian Journal of Microbiology 51: 765-771.
'Culture media and sources of nitrogen promoting the formation of stromata and ascocarps in Petromyces alliaceus (Aspergillus section Flavi) ' () 51 Canadian Journal of Microbiology : 765 -771.
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Mehl, H.L. and Cotty, P.J., 2013. Nutrient environments influence competition amongAspergillus flavus genotypes. Applied and Environmental Microbiology 79: 1473-1480.
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Moore, G.G., 2014. Sex and recombination in aflatoxigenic aspergilli: global implications. Frontiers in Food Microbiology 5: 1-5.
'Sex and recombination in aflatoxigenic aspergilli: global implications ' () 5 Frontiers in Food Microbiology : 1 -5.
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Moore, G.G., Mack, B.M. and Beltz, S.B., 2013. Testing the efficacy of eGFP-transformedAspergillus flavus as biocontrol strains. Food and Nutrition Sciences 4: 469-479.
'Testing the efficacy of eGFP-transformed Aspergillus flavus as biocontrol strains ' () 4 Food and Nutrition Sciences : 469 -479.
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Moore, G.G., Singh, R., Horn, B.W. and Carbone, I., 2009. Recombination and lineage-specific gene loss in the aflatoxin gene cluster ofAspergillus flavus. Molecular Ecology 18: 4870-4887.
'Recombination and lineage-specific gene loss in the aflatoxin gene cluster of Aspergillus flavus ' () 18 Molecular Ecology : 4870 -4887.
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Ni, M., Feretzaki, M., Sun, S., Wang, X. and Heitman, J., 2011. Sex in fungi. Annual Reviews of Genetics 45: 405-430.
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Olarte, R.A., Horn, B.W., Dorner, J.W., Monacell, J.T., Singh, R., Stone, E.A. and Carbone, I., 2012. Effect of sexual recombination on population diversity in aflatoxin production byAspergillus flavus and evidence for cryptic heterokaryosis. Molecular Ecology 21: 1453-1476.
'Effect of sexual recombination on population diversity in aflatoxin production by Aspergillus flavus and evidence for cryptic heterokaryosis ' () 21 Molecular Ecology : 1453 -1476.
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Runa, F., Carbone, I. and Payne, G.A., 2011. Nuclear condition inAspergillus flavus during growth and conidiation. In: Genetics of Maize Disease Workshop, Raleigh, NC, USA, February 20-23, 2011. Available at:http://www.pngg.org/maize/abstract.html
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Worthington, C.J., Horn, B.W., Moore, G.G., Monacell, J.T., Singh, R., Stone, E.A. and Carbone, I., 2011. Hybridization betweenAspergillus flavus andAspergillus parasiticus. In: 26th Fungal Genetics Conference, Pacific Grove, 15-20 March 2011. Available at:http://www.fgsc.net/26thFGC/26FGCProgramAndAbstracts.pdf
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|---|---|---|---|
| æè¦æµè§æ¬¡æ° | 198 | 79 | 12 |
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Heritability study of eGFP-transformedAspergillus flavus strains
äºWorld Mycotoxin JournalSearch for other papers by G.G. Moore in
Current site
Google Scholar
PubMed
Field inoculation with non-aflatoxigenicAspergillus flavus is a preferred method for pre-harvest biocontrol of aflatoxin contamination of maize, cottonseed, and groundnut. Rationale for using theseA. flavus strains is that they (1) maintain persistent control of aflatoxigenic fungi in the field, and (2) are incapable of out-crossing. Trackable field-released biocontrol strains will be beneficial to study the movement and longevity of non-aflatoxigenicA. flavus strains. Incorporating a naturally-occurring compound such as enhanced green fluorescent protein (eGFP) into a biocontrol strain might allow observation of its behaviour in field settings. The success of long-term field testing of eGFP-expressingA. flavus strains depends on their ability to maintain fluorescence throughout growth. Additionally, to ensure accurate tracking of the fluorescent atoxigenic strain, the likelihood of their out-crossing with individuals from the native population must be determined.In vitro mating experiments paired each of six different eGFP-transformed atoxigenic strains with a highly fertile toxigenicA. flavus isolate. Findings indicate that the eGFP gene, and possibly the aflatoxin cluster, is heritable by the F1 progeny. Not all cultured ascospores were fluorescent, but subsequent growth arising from a single fluorescent ascospore exhibited fluorescence similar to the eGFP parent. Observed mixed-fluorescence among conidia in a single chain suggests heterokaryosis at the moment of conidiogenesis. Mycotoxin assays showed that some fluorescent F1 individuals produce aflatoxin and/or cyclopiazonic acid which would indicate they are recombinant offspring. The findings in this laboratory study lend support to concern that atoxigenic strains are not impervious to genetic recombination and for which, if possible in a natural environment, repeated use could pose a risk of increasing the occurrence of aflatoxigenic individuals in treated fields.
| å ¨é¨æé´ | è¿å»ä¸å¹´ | è¿å»30天 | |
|---|---|---|---|
| æè¦æµè§æ¬¡æ° | 198 | 79 | 12 |
| å ¨ææµè§æ¬¡æ° | 40 | 0 | 0 |
| PDFä¸è½½æ¬¡æ° | 23 | 0 | 0 |
