In this study the effects of temperature, oxygen tension, water activity (aW), pH, incubation time and their interactions on (1) the lag phase prior to growth, (2) growth rate and (3) gliotoxin production of two feed-borneAspergillus fumigatus sensu stricto strains, isolated from fermented maize silage and brewer's grains, were evaluated on an agar medium based on these substrates. Regardless of oxygen tension, the growth rate of the two strains decreased significantly as temperature and aW decreased (P<0.05). The optimum conditions forA. fumigatus growth were 37 °C, 0.98 aW for both strains at reduced oxygen tension, regardless of pH level (P<0.05). The studiedA. fumigatus strains were able to grow under several incubation conditions, some of them prevalent in stored animal feeds. Some specific interactions that allowed accumulation of gliotoxin at high levels were found. This study showed that gliotoxin production occurred at more restricted conditions than fungal growth. This fact is important, as by maintaining the appropriate conditions in animal feeds,A. fumigatus growth and gliotoxin production can be prevented. In this study, growth rates, lag phases prior to growth and gliotoxin production over a range of environmental conditions provide useful information that can help in predicting the possible fungal contamination of fermented animal feeds. Furthermore, the information is relevant sinceA. fumigatus is an opportunistic pathogen found in cereals and fermented animal feeds and represents a high risk of contamination to animals and farm workers who handle them improperly.
Purchase
Buy instant access (PDF download and unlimited online access):
Institutional Login
Log in with Open Athens, Shibboleth, or your institutional credentials
Personal login
Log in with your brill.com account
Abrunhosa, L., Serra, R. and Venâncio, A., 2002. Biodegradation of ochratoxin A by fungi isolated from grapes. Journal of Agricultural and Food Chemistry 50: 7493-7496.
'Biodegradation of ochratoxin A by fungi isolated from grapes ' () 50 Journal of Agricultural and Food Chemistry : 7493 -7496.
Bauer, J., Gareis, M., Bott, A. and Gedek, B., 1989. Isolation of a mycotoxin (gliotoxin) from a bovine udder infected withAspergillus fumigatus. Journal of Medical and Veterinary Mycology 27: 45-50.
'Isolation of a mycotoxin (gliotoxin) from a bovine udder infected with Aspergillus fumigatus ' () 27 Journal of Medical and Veterinary Mycology : 45 -50.
Bellí, N., Marín, S., Sanchis, V. and Ramos, A.J., 2004. Influence of water activity and temperature on growth of isolates ofAspergillus sectionNigri obtained from grapes. International Journal of Food Microbiology 96: 19-27.
'Influence of water activity and temperature on growth of isolates of Aspergillus section Nigri obtained from grapes ' () 96 International Journal of Food Microbiology : 19 -27.
Boysen, M.E., Jacobsson, K.G. and Schnürer, J., 2000. Molecular identification of species from thePenicillium roqueforti group associated with spoiled animal feed. Applied and Environmental Microbiology 66: 1523-1526.
'Molecular identification of species from the Penicillium roqueforti group associated with spoiled animal feed ' () 66 Applied and Environmental Microbiology : 1523 -1526.
Cavaglieri, L.R., González Pereyra, M.L., Pereyra, C.M., Magnoli, C.E., Chulze, S.N. and Dalcero, A.M., 2005. Fungal and mycotoxin contamination of cow feeding stuffs in Argentina. In: Conference on reducing impact of mycotoxins in tropical agriculture. Ghana, Africa, abstract.
Dallyn, H. and Fox, A., 1980. Spoilage of material of reduced water activity by xerophilic fungi. In: Gould, G.H. and Corry, E.L. (eds.) Microbial growth and survival in extreme environments. Academic Press, London, UK, pp. 129-139.
Microbial growth and survival in extreme environments , () 129 -139.
Daly, P. and Kavanagh K, 2001. Pulmonary aspergillosis: clinical presentation, diagnosis and therapy. British Journal of Biomedical Science 58: 197-205.
'Pulmonary aspergillosis: clinical presentation, diagnosis and therapy ' () 58 British Journal of Biomedical Science : 197 -205.
Denning, D.W., 1998. Invasive aspergillosis. Clinical Infectious Diseases 26: 781-803.
'Invasive aspergillosis ' () 26 Clinical Infectious Diseases : 781 -803.
Faryal, R., Lodhi, A. and Hameed, A., 2006. Isolation, characterization and biosorption of zinc by indigenous fungal strainsAspergillus fumigatus RH05 andAspergillus flavus RH07. Pakistan Journal of Botany 38: 817-832.
'Isolation, characterization and biosorption of zinc by indigenous fungal strains Aspergillus fumigatus RH05 and Aspergillus flavus RH07 ' () 38 Pakistan Journal of Botany : 817 -832.
Frisvad, J.C., 1987. High-performance liquid chromatographic determination of profiles of mycotoxins and other secondary metabolites. Journal of Chromatography A 392: 333-347.
'High-performance liquid chromatographic determination of profiles of mycotoxins and other secondary metabolites ' () 392 Journal of Chromatography A : 333 -347.
García, D., Ramos, A.J., Sanchís, V. and Marín, S., 2009. Predicting mycotoxins in foods: a review. Food Microbiology 26: 757-769.
'Predicting mycotoxins in foods: a review ' () 26 Food Microbiology : 757 -769.
Gareis, M. and Wernery, U., 1994. Determination of gliotoxin in samples associated with cases of intoxication in camels. Mycotoxin Research 10: 2-8.
'Determination of gliotoxin in samples associated with cases of intoxication in camels ' () 10 Mycotoxin Research : 2 -8.
Geisen, R., 1996. Multiplex polymerase chain reaction for the detection of potential aflatoxin and sterigmatocystin producing fungi. Journal of Applied Microbiology 19: 388-392.
'Multiplex polymerase chain reaction for the detection of potential aflatoxin and sterigmatocystin producing fungi ' () 19 Journal of Applied Microbiology : 388 -392.
Glass, N.L. and Donaldson, G., 1995. Development of primer 406 sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. Applied and Environmental Microbiology 61: 1323-1330.
'Development of primer 406 sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes ' () 61 Applied and Environmental Microbiology : 1323 -1330.
González Pereyra, M.L., Alonso, V.A., Sager, R., Morlaco, M.B., Magnoli, C.E., Astoreca, A.L., Rosa, C.A.R., Chiacchiera, S.M., Dalcero, A.M. and Cavaglieri, L.R., 2008. Fungi and selected mycotoxins from pre- and post-fermented corn silage. Journal of Applied Microbiology 104: 1034-1041.
'Fungi and selected mycotoxins from pre- and post-fermented corn silage ' () 104 Journal of Applied Microbiology : 1034 -1041.
González Pereyra, M.L., Chiacchiera, S.M., Rosa, C.A.R., Sager, R., Dalcero, A.M. and Cavaglieri, L., 2011. Comparative analysis of the mycobiota and mycotoxins contaminating corn trench silos and silo bags. Journal of the Science of Food and Agriculture 91: 1474-1481.
'Comparative analysis of the mycobiota and mycotoxins contaminating corn trench silos and silo bags ' () 91 Journal of the Science of Food and Agriculture : 1474 -1481.
Gordon, K.E., Masotti, R.E. and Waddell, W.R., 1993. Tremorgenic encephalopathy: a role of mycotoxins in the production of CNS disease in humans. Canadian Journal of Neurological Sciences 20: 237-239.
'Tremorgenic encephalopathy: a role of mycotoxins in the production of CNS disease in humans ' () 20 Canadian Journal of Neurological Sciences : 237 -239.
Hall, L.A. and Denning, D.W., 1994. Oxygen requirements ofAspergillus species. Journal of Medical Microbiology 41: 311-315.
'Oxygen requirements of Aspergillus species ' () 41 Journal of Medical Microbiology : 311 -315.
Hong, S.B., Kim, D.H., Park, I.C., Choi, Y.J., Shin, H.D. and Samson, R.A., 2010. Re-identification ofAspergillus fumigatus sensu lato based on a new concept of species delimitation. Journal of Microbiology 48: 607-615.
'Re-identification of Aspergillus fumigatus sensu lato based on a new concept of species delimitation ' () 48 Journal of Microbiology : 607 -615.
Kupfahl, C., Heinekamp, T., Geginat, G., Ruppert, T., Hartl, A., Hof, H. and Brakhage, A.A., 2006. Deletion of thegliP gene ofAspergillus fumigatus results in loss of gliotoxin production but has no effect on virulence of the fungus in a low-dose mouse infection model. Molecular Microbiology 62: 292-302.
'Deletion of the gliP gene of Aspergillus fumigatus results in loss of gliotoxin production but has no effect on virulence of the fungus in a low-dose mouse infection model ' () 62 Molecular Microbiology : 292 -302.
Land, C., Hult, K., Fuchs, R., Hagelberg, S. and Lundstrom, H., 1987. Tremorgenic mycotoxins fromAspergillus fumigatus as a possible occupational health problem in sawmills. Applied and Environmental Microbiology 193: 262-275.
'Tremorgenic mycotoxins from Aspergillus fumigatus as a possible occupational health problem in sawmills ' () 193 Applied and Environmental Microbiology : 262 -275.
Latgé, J.P., Verweij, P.E. and Bretagne, S., 2000. Molecular typing ofAspergillus fumigatus. In: Samson, R.A. and Pitt, J.I. (eds.) Integration of modern taxonomic methods forPenicillium andAspergillus classification. Harwood Academic Publishers, Amsterdam, the Netherlands, pp. 471-482.
Integration of modern taxonomic methods for Penicillium and Aspergillus classification , () 471 -482.
Leong, S.L., Hocking, A.D. and Scott, E.S., 2006. Effect of temperature and water activity on growth and ochratoxin A production by AustralianAspergillus carbonarius andA. niger isolates on a simulated grape juice medium. International Journal of Food Microbiology 110: 209-216.
'Effect of temperature and water activity on growth and ochratoxin A production by Australian Aspergillus carbonarius and A. niger isolates on a simulated grape juice medium ' () 110 International Journal of Food Microbiology : 209 -216.
Lewis, R.E., Wiederhold, N.P., Chi, J., Han, X.Y., Komanduri, K.V., Kontoyiannis, D.P. and Prince, R.A., 2005. Detection of gliotoxin in experimental and human aspergillosis. Infection and Immunity 73: 635-637.
'Detection of gliotoxin in experimental and human aspergillosis ' () 73 Infection and Immunity : 635 -637.
Magan, N. and Lacey, J., 1984. Effect of temperature and pH on water relations of field and storage fungi. Transactions of the British Mycological Society 82: 71-81.
'Effect of temperature and pH on water relations of field and storage fungi ' () 82 Transactions of the British Mycological Society : 71 -81.
Nieminen, S.M., Maki-Paakkanen, J., Hirvonen, M.R., Roponen, M. and Von Wright, A., 2002. Genotoxicity of gliotoxin, a secondary metabolite ofAspergillus fumigatus, in a battery of short-term test systems. Mutation Research 520: 161-170.
'Genotoxicity of gliotoxin, a secondary metabolite of Aspergillus fumigatus, in a battery of short-term test systems ' () 520 Mutation Research : 161 -170.
Northolt, M.D., Frisvad, J.C. and Samson, R.A., 1995. Occurrence of food-borne fungi and factors for growth. In: Samson, R.A., Hoekstra, E.S., Frisvad, J.C. and Filtenborg, O. (eds.) Introduction to food-borne fungi. Centraalbureau voor Schimmelcultures, Baarn, the Netherlands, pp. 243-250.
Introduction to food-borne fungi , () 243 -250.
Nwagu, T.N. and Okolo, B.N., 2011. Growth profile and amylolytic activity of a thermophilic fungusAspergillus fumigatus isolated from soil. Asian Journal of Biotechnology 3: 46-57.
'Growth profile and amylolytic activity of a thermophilic fungus Aspergillus fumigatus isolated from soil ' () 3 Asian Journal of Biotechnology : 46 -57.
Paisley, D., Robson, G.D. and Denning, D.W., 2005. Correlation betweenin vitro growth rate andin vivo virulence inAspergillus fumigatus. Medical Mycology 43: 397-401.
'Correlation between in vitro growth rate and in vivo virulence in Aspergillus fumigatus ' () 43 Medical Mycology : 397 -401.
Pereyra, C.M., Alonso, V.A., Rosa, C.A.R., Chiacchiera, S.M., Dalcero, A.M. and Cavaglieri, L.R., 2008. Gliotoxin natural incidence ofAspergillus fumigatus isolated from corn silage and ready dairy cattle feed. World Mycotoxin Journal 1: 457-462.
'Gliotoxin natural incidence of Aspergillus fumigatus isolated from corn silage and ready dairy cattle feed ' () 1 World Mycotoxin Journal : 457 -462.
Samson, R.A., Hoekstra, E.S. and Frisvad, J.C., 2004b. Introduction to food and airborne fungi, 7th edition. Centraalbureau voor Schimmelcultures, Utrecht, the Netherlands.
Introduction to food and airborne fungi , ().
Samson, R.A., Hong, S., Peterson, S.W., Frisvad, J.C. and Varga, J., 2007. Polyphasic taxonomy ofAspergillus sectionFumigati and its teleomorphNeosartorya. Studies in Mycology 59: 147-203.
'Polyphasic taxonomy of Aspergillus section Fumigati and its teleomorph Neosartorya ' () 59 Studies in Mycology : 147 -203.
Samson, R.A., Houbraken, J.A.M.P., Kuijpers, A.F.A., Frank, J.M. and Frisvad, J.C., 2004a. New ochratoxin A or sclerotium producing species inAspergillus sectionNigri. Studies in Mycology 50: 45-61.
'New ochratoxin A or sclerotium producing species in Aspergillus section Nigri ' () 50 Studies in Mycology : 45 -61.
Scharf, D.H., Heinekamp, T., Remme, N., Hortschansky, P., Brakhage, A.A. and Hertweck, C., 2012. Biosynthesis and function of gliotoxin inAspergillus fumigatus. Applied Microbiology and Biotechnology 93: 467-472.
'Biosynthesis and function of gliotoxin in Aspergillus fumigatus ' () 93 Applied Microbiology and Biotechnology : 467 -472.
Stanzani, M., Orciuolo, E., Lewis, R., Kontoyiannis, D., Martins, S., St. John, L. and Komanduri, K., 2005.Aspergillus fumigatus suppresses the human cellular immune response via gliotoxin-mediated apoptosis of monocytes. Blood 105: 2258-2265.
'Aspergillus fumigatus suppresses the human cellular immune response via gliotoxin-mediated apoptosis of monocytes ' () 105 Blood : 2258 -2265.
Sulyok, M., Krska, R. and Schuhmacher, R., 2007. A liquid chromatography/tandem mass spectrometric multimycotoxin method for the quantification of 87 analytes and its application to semi-quantitative screening of moldy food samples. Analytical and Bioanalytical Chemistry 389: 1505-1523.
'A liquid chromatography/tandem mass spectrometric multimycotoxin method for the quantification of 87 analytes and its application to semi-quantitative screening of moldy food samples ' () 389 Analytical and Bioanalytical Chemistry : 1505 -1523.
Tepsic, K., Gunde-Cimerman, N. and Frisvad, J.C., 1997. Growth and mycotoxin production byAspergillus fumigatus strains isolated from a saltern. FEMS Microbiology Letters 157: 9-12.
'Growth and mycotoxin production by Aspergillus fumigatus strains isolated from a saltern ' () 157 FEMS Microbiology Letters : 9 -12.
Upperman, J.S., Potoka, D.A., Zhang, X.R., Wong, K., Zamora, R. and Ford, H.R., 2003. Mechanism of intestinalderived fungal sepsis by gliotoxin, a fungal metabolite. Journal of Pediatric Surgery 38: 966-970.
'Mechanism of intestinalderived fungal sepsis by gliotoxin, a fungal metabolite ' () 38 Journal of Pediatric Surgery : 966 -970.
Varga, J., Kevei, F., Hamari, Z., Tóth, B. and Téren, J., 2000. Genotypic and phenotypic variability among blackAspergilli. In: Samson, R.A. and Pitt, J.I. (eds.) Integration of modern taxonomic methods forPenicillium andAspergillus classification. Harwood Academic Publishers, Amsterdam, the Netherlands, pp. 397-411.
Integration of modern taxonomic methods for Penicillium and Aspergillus classification , () 397 -411.
Waring, P., Eichner, R., Mullbacher, A. and Sjaarda, A., 1988. Gliotoxin induces apoptosis in macrophages unrelated to its antiphagocytic properties. Journal of Biological Chemistry 263: 18493-18499.
'Gliotoxin induces apoptosis in macrophages unrelated to its antiphagocytic properties ' () 263 Journal of Biological Chemistry : 18493 -18499.
Watanabe, A., Kamei, K., Sekine, T., Waku, M., Nishimura, K. and Miyaji, M., 2004. Effect of aeration on gliotoxin production byAspergillus fumigatus in its culture filtrate. Mycopathologia 157: 245-254.
'Effect of aeration on gliotoxin production by Aspergillus fumigatus in its culture filtrate ' () 157 Mycopathologia : 245 -254.
Willger, S.D., Grahl, N. and Cramer, Jr., R.A., 2009.Aspergillus fumigatus metabolism: clues to mechanisms ofin vivo fungal growth and virulence. Medical Mycology 47: S72-S79.
'Aspergillus fumigatus metabolism: clues to mechanisms of in vivo fungal growth and virulence ' () 47 Medical Mycology : S72 -S79.
Woo Bok, J., Woon Chung, D., Balajee, S.A., Marr, K.A., Andes, D., Nielsen, K.F. and Frisvad, J.C., 2006. GliZ, a transcriptional regulator of gliotoxin biosynthesis, contributes toAspergillus fumigatus virulence. Infection and Immunity 74: 6761-6768.
'GliZ, a transcriptional regulator of gliotoxin biosynthesis, contributes to Aspergillus fumigatus virulence ' () 74 Infection and Immunity : 6761 -6768.
| All Time | Past 365 days | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 199 | 92 | 12 |
| Full Text Views | 23 | 1 | 0 |
| PDF Views & Downloads | 8 | 0 | 0 |
In this study the effects of temperature, oxygen tension, water activity (aW), pH, incubation time and their interactions on (1) the lag phase prior to growth, (2) growth rate and (3) gliotoxin production of two feed-borneAspergillus fumigatus sensu stricto strains, isolated from fermented maize silage and brewer's grains, were evaluated on an agar medium based on these substrates. Regardless of oxygen tension, the growth rate of the two strains decreased significantly as temperature and aW decreased (P<0.05). The optimum conditions forA. fumigatus growth were 37 °C, 0.98 aW for both strains at reduced oxygen tension, regardless of pH level (P<0.05). The studiedA. fumigatus strains were able to grow under several incubation conditions, some of them prevalent in stored animal feeds. Some specific interactions that allowed accumulation of gliotoxin at high levels were found. This study showed that gliotoxin production occurred at more restricted conditions than fungal growth. This fact is important, as by maintaining the appropriate conditions in animal feeds,A. fumigatus growth and gliotoxin production can be prevented. In this study, growth rates, lag phases prior to growth and gliotoxin production over a range of environmental conditions provide useful information that can help in predicting the possible fungal contamination of fermented animal feeds. Furthermore, the information is relevant sinceA. fumigatus is an opportunistic pathogen found in cereals and fermented animal feeds and represents a high risk of contamination to animals and farm workers who handle them improperly.
| All Time | Past 365 days | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 199 | 92 | 12 |
| Full Text Views | 23 | 1 | 0 |
| PDF Views & Downloads | 8 | 0 | 0 |