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By-products of grain cleaning: an opportunity for rapid sampling and screening of wheat for mycotoxins

In: World Mycotoxin Journal
Authors:
S.A. Tittlemier Grain Research Laboratory, Canadian Grain Commission, 1404-303 Main Street, Winnipeg, MB R3C 3G8, Canada

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D. Sobering Industry Services, Canadian Grain Commission, 900-303 Main Street, Winnipeg, MB R3C 3G8, Canada

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K. Bowler Industry Services, Canadian Grain Commission, 900-303 Main Street, Winnipeg, MB R3C 3G8, Canada

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T. Zirdum Industry Services, Canadian Grain Commission, 900-303 Main Street, Winnipeg, MB R3C 3G8, Canada

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D. Gaba Grain Research Laboratory, Canadian Grain Commission, 1404-303 Main Street, Winnipeg, MB R3C 3G8, Canada

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J.M. Chan Grain Research Laboratory, Canadian Grain Commission, 1404-303 Main Street, Winnipeg, MB R3C 3G8, Canada

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M. Roscoe Grain Research Laboratory, Canadian Grain Commission, 1404-303 Main Street, Winnipeg, MB R3C 3G8, Canada

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R. Blagden Grain Research Laboratory, Canadian Grain Commission, 1404-303 Main Street, Winnipeg, MB R3C 3G8, Canada

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L. Campbell National Monitoring Unit, Canadian Grain Commission, 1404-303 Main Street, Winnipeg, MB R3C 3G8, Canada

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By-products of cereal grain cleaning were analysed for a number of mycotoxins. Deoxynivalenol (DON) was the most frequently detected in by-products from commercial-scale cleaning procedures (maximum 2.94 mg/kg), followed by zearalenone (ZEA; maximum 0.045 mg/kg) and ochratoxin A (OTA; maximum 0.019 mg/kg). These three mycotoxins were also the most frequently detected in four different fractions collected from wheat run through a dockage tester, a piece of equipment used in the Canadian inspection process to separate material other than grain from wheat. Concentrations of mycotoxins were highest in the ‘light dockage’ fraction that contained dust and roughage such as glumes, fragments of stem, or rachis. Mycotoxin concentrations in this fraction reached up to 32 mg/kg (DON), 0.532 mg/kg (ZEA), and 0.249 mg/kg (OTA). Concentrations of DON in light dockage were significantly correlated with concentrations in whole grain that was un-cleaned or had undergone basic cleaning, indicating that the light dockage fraction could be used as a readily available matrix for the rapid screening of DON in wheat. This would eliminate the time required for additional sampling and preparation of whole grain, and move towards a truly rapid method for the screening of DON in wheat.

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