The aim of this study was to examine three strains of the yeastSaccharomyces cerevisiae and three strains of lactic acid bacteria belonging to the genusLactobacillus for their antifungal activity against the ochratoxin A producerAspergillus westerdijkiae, as well as for their effect on OTA genotoxicity and cytotoxicity. When inoculated simultaneously, fungal growth was completely inhibited byS. cerevisiae. In the case of lactic acid bacteria, growth inhibition also occurred but to a less extent. A significant decrease in toxin production in co-culture with the yeast strains and LAB was observed. The supernatant of 24-h-old cultures of yeast strains in medium with OTA did not influence significantly the viability of porcine kidney epithelial LLC-PK1 cell line, whereas the supernatant from the LAB increased the viability compared to the control. Regarding genotoxicity, a decreased fragmentation of DNA was observed in the presence of the supernatant from wine and brewing yeasts, andLactobacillus brevis strains. Based on the results obtained, it might be concluded thatS. cerevisiae yeasts and lactic acid bacteria could be used to minimise the negative effect of OTA on humans and animals.
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The aim of this study was to examine three strains of the yeastSaccharomyces cerevisiae and three strains of lactic acid bacteria belonging to the genusLactobacillus for their antifungal activity against the ochratoxin A producerAspergillus westerdijkiae, as well as for their effect on OTA genotoxicity and cytotoxicity. When inoculated simultaneously, fungal growth was completely inhibited byS. cerevisiae. In the case of lactic acid bacteria, growth inhibition also occurred but to a less extent. A significant decrease in toxin production in co-culture with the yeast strains and LAB was observed. The supernatant of 24-h-old cultures of yeast strains in medium with OTA did not influence significantly the viability of porcine kidney epithelial LLC-PK1 cell line, whereas the supernatant from the LAB increased the viability compared to the control. Regarding genotoxicity, a decreased fragmentation of DNA was observed in the presence of the supernatant from wine and brewing yeasts, andLactobacillus brevis strains. Based on the results obtained, it might be concluded thatS. cerevisiae yeasts and lactic acid bacteria could be used to minimise the negative effect of OTA on humans and animals.
| All Time | Past 365 days | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 280 | 130 | 5 |
| Full Text Views | 16 | 1 | 0 |
| PDF Views & Downloads | 14 | 1 | 0 |