Occurrence of mono- and di-glycosylated conjugates of T-2 and HT-2 toxins in naturally contaminated cereals
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An increasing incidence of T-2 and HT-2 toxins (T-2 and HT-2) representing group A trichothecenes has been observed in Fusarium infected small grain cereals in the last decade. Quite recently, the existence of glucosides of T-2 and HT-2 has also been proved next to the common parent forms. There is a strong desire to deepen the knowledge of the occurrence of these conjugates and the possibilities of their analytical determination. A new analytical procedure has been developed for monitoring T-2/HT-2 conjugates in cereal samples. Four different sample preparation methods based on crude acetonitrile:water extraction, QuEChERS, solid phase extraction and immunoaffinity clean-up, were tested. The latter approach employing dedicated immunoaffinity cross-reactive cartridges was shown to be the best option for selective isolation and pre-concentration of the target conjugated analytes. The samples obtained in this way were examined by ultra-high performance liquid chromatography hyphenated to high resolution tandem mass spectrometry that enabled the confirmation of the presence of conjugated T-2 and HT-2. In addition to mono-glycosylated forms of T-2 and HT-2 detected in naturally contaminated barley, wheat and oats, we have also documented for the first time the existence of diglucosides of HT-2 in barley.
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Berthiller, F., Krska, R., Domig, K.J., Kneifel, W., Juge, N., Schuhmacher, R. and Adam, G., 2011. Hydrolytic fate of deoxynivalenol-3-glucoside during digestion. Toxicology Letters 206: 264-267.
'Hydrolytic fate of deoxynivalenol-3-glucoside during digestion ' () 206 Toxicology Letters : 264 -267 .
-
Berthiller, F., Schuhmacher, R., Adam, G. and Krska, R., 2009. Formation, determination and significance of masked and other conjugated mycotoxins. Analytical and Bioanalytical Chemistry 395: 1243-1252.
'Formation, determination and significance of masked and other conjugated mycotoxins ' () 395 Analytical and Bioanalytical Chemistry : 1243 -1252 .
-
Busman, M., Poling, S.M. and Maragos, C.M., 2011. Observation of T-2 toxin and HT-2 toxin glucosides from Fusarium sporotrichoides by liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). Toxins 3: 1554-1568.
'Observation of T-2 toxin and HT-2 toxin glucosides from Fusarium sporotrichoides by liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) ' () 3 Toxins : 1554 -1568 .
-
Cano-Sancho, G., Valle-Algarra, F.M., Jiménez, M., Burdaspal, P., Legarda, T.M., Ramos, A.J., Sanchis, V. and Marín, S., 2011. Presence of trichothecenes and co-occurrence in cereal-based food from Catalonia (Spain). Food Control 22: 490-495.
'Presence of trichothecenes and co-occurrence in cereal-based food from Catalonia (Spain) ' () 22 Food Control : 490 -495 .
-
EFSA Panel on Contaminants in the Food Chain (CONTAM), 2011. Scientific opinion on the risks for animal and public health related to the presence of T-2 and HT-2 toxin in food and feed. EFSA Journal 9: 2481.
-
European Commission (EC), 2006. Commission Regulation (EC) No. 1881/2006 of 19 December 2006 setting maximum levels for certain contaminants in foodstuffs. Official Journal of the European Union L364: 5-24.
-
Kostelanska, M., Zachariasova, M., Lacina, O., Fenclova, M., Kollos, A.-L. and Hajslova, J., 2011. The study of deoxynivalenol and its masked metabolites fate during the brewing process realised by UPLC-TOFMS method. Food chemistry 126: 1870-1876.
'The study of deoxynivalenol and its masked metabolites fate during the brewing process realised by UPLC-TOFMS method ' () 126 Food chemistry : 1870 -1876 .
-
Lattanzio, V.M.T., Visconti, A., Haidukowski, M. and Pascale, M., 2012. Identification and characterization of new Fusarium masked mycotoxins, T2 and HT2 glycosyl derivatives, in naturally contaminated wheat and oats by liquid chromatography-high-resolution mass spectrometry. Journal of Mass Spectrometry 47: 466-475.
'Identification and characterization of new Fusarium masked mycotoxins, T2 and HT2 glycosyl derivatives, in naturally contaminated wheat and oats by liquid chromatography-high-resolution mass spectrometry ' () 47 Journal of Mass Spectrometry : 466 -475 .
-
Malachova, A., Dzuman, Z., Veprikova, Z., Vaclavikova, M., Zachariasova, M. and Hajslova, J., 2011. Deoxynivalenol, deoxynivalenol-3-glucoside, and enniatins: the major mycotoxins found in cereal-based products on the Czech market. Journal of Agricultural and Food Chemistry 59: 12990-12997.
'Deoxynivalenol, deoxynivalenol-3-glucoside, and enniatins: the major mycotoxins found in cereal-based products on the Czech market ' () 59 Journal of Agricultural and Food Chemistry : 12990 -12997 .
-
Nakagawa, H., Ohmichi, K., Sakamoto, S., Sago, Y., Kushiro, M., Nagashima, H., Yoshida, M. and Nakajima, T., 2011. Detection of new Fusarium masked mycotoxins in wheat grain by high resolution LC-Orbitrap MS. Food Additives and Contaminants 28 Part A: 1447-1456.
'Detection of new Fusarium masked mycotoxins in wheat grain by high resolution LC-Orbitrap MS ' () Food Additives and Contaminants 28 Part A : 1447 -1456 .
-
Pascale, M., Panzarini, G. and Visconti, A., 2012. Determination of HT-2 and T-2 toxins in oats and wheat by ultra-performance liquid chromatography with photodiode array detection. Talanta 89: 231-236.
'Determination of HT-2 and T-2 toxins in oats and wheat by ultra-performance liquid chromatography with photodiode array detection ' () 89 Talanta : 231 -236. .
-
Pettersson, H., Brown, C., Hauk, J., Hoth, S., Meyer, J. and Wessels, D., 2011. Survey of T-2 and HT-2 toxins by LC-MS/MS in oats and oat products from European oat mills in 2005-2009. Food Additives and Contaminants Part B 4: 110-115.
'Survey of T-2 and HT-2 toxins by LC-MS/MS in oats and oat products from European oat mills in 2005-2009 ' () 4 Food Additives and Contaminants Part B : 110 -115 .
-
Poppenberger, B., Berthiller, F., Lucyshyn, D., Sieberer, T., Schuhmacher, R., Krska, R., Kuchler, K., Glossl, J., Luschnig, C. and Adam G., 2003. Detoxification of the Fusarium mycotoxin deoxynivalenol by a UDP-glucosyltransferase from Arabidopsis thaliana. Journal of Biological Chemistry 278: 47905-47914.
'Detoxification of the Fusarium mycotoxin deoxynivalenol by a UDP-glucosyltransferase from Arabidopsis thaliana ' () 278 Journal of Biological Chemistry : 47905 -47914 .
-
Rahmani, A., Jinap, S. and Soleimany, F., 2009. Qualitative and quantitative analysis of mycotoxins. Comprehensive Reviews in Food Science and Food Safety 8: 202-251.
'Qualitative and quantitative analysis of mycotoxins ' () 8 Comprehensive Reviews in Food Science and Food Safety : 202 -251 .
-
Sulyok, M., Kska, R. and Schuhmacher, R., 2007. A liquid chromatography/tandem mass spectrometric multi-mycotoxin method for the quantification of 87 analytes and its application to semi-quantitative screening of moldy food samples. Analytical and Bioanalytical Chemistry 389: 1505-1523.
'A liquid chromatography/tandem mass spectrometric multi-mycotoxin method for the quantification of 87 analytes and its application to semi-quantitative screening of moldy food samples ' () 389 Analytical and Bioanalytical Chemistry : 1505 -1523 .
-
Trebstein, A., Seefelder, W., Lauber, U. and Humpf, H.-U., 2008. Determination of T-2 and HT-2 toxins in cereals including oats after immunoaffinity cleanup by liquid chromatography and fluorescence detection. Journal of Agricultural and Food Chemistry 56: 4968-4975.
'Determination of T-2 and HT-2 toxins in cereals including oats after immunoaffinity cleanup by liquid chromatography and fluorescence detection ' () 56 Journal of Agricultural and Food Chemistry : 4968 -4975 .
-
Vendl, O., Berthiller, F., Crews, C. and Krska, R., 2009. Simultaneous determination of deoxynivalenol, zearalenone, and their major masked metabolites in cereal-based food by LC-MS-MS. Analytical and Bioanalytical Chemistry 395: 1347-1354.
'Simultaneous determination of deoxynivalenol, zearalenone, and their major masked metabolites in cereal-based food by LC-MS-MS ' () 395 Analytical and Bioanalytical Chemistry : 1347 -1354 .
-
Zachariasova, M., Lacina, O., Malachova, A., Kostelanska, M., Poustka, J., Godula, M. and Hajslova, J., 2010. Novel approaches in analysis of Fusarium mycotoxins in cereals employing ultra-performance liquid chromatography coupled with high resolution mass spectrometry. Analytica Chimica Acta 662: 51-61.
'Novel approaches in analysis of Fusarium mycotoxins in cereals employing ultra-performance liquid chromatography coupled with high resolution mass spectrometry ' () 662 Analytica Chimica Acta : 51 -61 .
| All Time | Past 365 days | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 281 | 59 | 3 |
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Occurrence of mono- and di-glycosylated conjugates of T-2 and HT-2 toxins in naturally contaminated cereals
In: World Mycotoxin JournalSearch for other papers by Z. Veprikova in
Current site
Google Scholar
PubMed
Search for other papers by M. Vaclavikova in
Current site
Google Scholar
PubMed
Search for other papers by O. Lacina in
Current site
Google Scholar
PubMed
Search for other papers by Z. Dzuman in
Current site
Google Scholar
PubMed
Search for other papers by M. Zachariasova in
Current site
Google Scholar
PubMed
Search for other papers by J. Hajslova in
Current site
Google Scholar
PubMed
An increasing incidence of T-2 and HT-2 toxins (T-2 and HT-2) representing group A trichothecenes has been observed in Fusarium infected small grain cereals in the last decade. Quite recently, the existence of glucosides of T-2 and HT-2 has also been proved next to the common parent forms. There is a strong desire to deepen the knowledge of the occurrence of these conjugates and the possibilities of their analytical determination. A new analytical procedure has been developed for monitoring T-2/HT-2 conjugates in cereal samples. Four different sample preparation methods based on crude acetonitrile:water extraction, QuEChERS, solid phase extraction and immunoaffinity clean-up, were tested. The latter approach employing dedicated immunoaffinity cross-reactive cartridges was shown to be the best option for selective isolation and pre-concentration of the target conjugated analytes. The samples obtained in this way were examined by ultra-high performance liquid chromatography hyphenated to high resolution tandem mass spectrometry that enabled the confirmation of the presence of conjugated T-2 and HT-2. In addition to mono-glycosylated forms of T-2 and HT-2 detected in naturally contaminated barley, wheat and oats, we have also documented for the first time the existence of diglucosides of HT-2 in barley.
| All Time | Past 365 days | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 281 | 59 | 3 |
| Full Text Views | 53 | 1 | 0 |
| PDF Views & Downloads | 16 | 0 | 0 |
