Use of solid phase microextraction coupled to capillary gas chromatography-mass spectrometry for screening Fusarium spp. based on their volatile sesquiterpenes
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Solid phase microextraction (SPME) coupled to capillary gas chromatography (CGC) and mass spectrometry (MS) was used to evaluate the use of fungal volatiles to discriminate Fusarium species from wheat cultivars in the Argentina pampa region. Monosporic fungal isolates were grown on rice in sealed containers for 1 week and volatile organic compounds (VOC) were sampled for 30 min from the head space by SPME and analysed by CGC and CGCMS. VOC profiles of Fusarium species F. graminearum, F. poae, F. equiseti, F. verticillioides and F. oxysporum were discriminated by comparison of their profiles in the elution zone corresponding to sesquiterpenes. Trichothecene-producer and non-trichothecene producer Fusarium species were separated by the presence of trichodiene in their VOC fingerprints. Within trichothecene-producers, F. graminearum, F. poae and F. equiseti differed on the structure of their volatile sesquiterpenes. This technique might be also helpful to detect F. graminearum, the major head blight disease-producing fungus in the region.
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Use of solid phase microextraction coupled to capillary gas chromatography-mass spectrometry for screening Fusarium spp. based on their volatile sesquiterpenes
In: World Mycotoxin JournalSearch for other papers by J. Girotti in
Current site
Google Scholar
PubMed
Search for other papers by I. Malbrán in
Current site
Google Scholar
PubMed
Search for other papers by G. Lori in
Current site
Google Scholar
PubMed
Search for other papers by M. Juárez in
Current site
Google Scholar
PubMed
Solid phase microextraction (SPME) coupled to capillary gas chromatography (CGC) and mass spectrometry (MS) was used to evaluate the use of fungal volatiles to discriminate Fusarium species from wheat cultivars in the Argentina pampa region. Monosporic fungal isolates were grown on rice in sealed containers for 1 week and volatile organic compounds (VOC) were sampled for 30 min from the head space by SPME and analysed by CGC and CGCMS. VOC profiles of Fusarium species F. graminearum, F. poae, F. equiseti, F. verticillioides and F. oxysporum were discriminated by comparison of their profiles in the elution zone corresponding to sesquiterpenes. Trichothecene-producer and non-trichothecene producer Fusarium species were separated by the presence of trichodiene in their VOC fingerprints. Within trichothecene-producers, F. graminearum, F. poae and F. equiseti differed on the structure of their volatile sesquiterpenes. This technique might be also helpful to detect F. graminearum, the major head blight disease-producing fungus in the region.
| All Time | Past 365 days | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 130 | 46 | 4 |
| Full Text Views | 21 | 0 | 0 |
| PDF Views & Downloads | 15 | 0 | 0 |
