Inositol-6-phosphate mitigates deoxynivalenol nephrotoxicity in pig kidney explants
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Abstract
Deoxynivalenol (DON) is a mycotoxin that frequently contaminates food crops and negatively impacts human and animal health. While inositol-6-phosphate (IP6) is known to counteract DON-induced intestinal damage, inflammation, and oxidative stress, its ability to alleviate DON-caused kidney injury is yet unexplored. This study assessed DON’s kidney effects and IP6’s protective capability using an ex vivo renal explants model. From 360 explants taken from six pigs, four groups were formed: control (culture medium), DON (10 μM equivalent to 3 mg/kg of feed), IP6 group (5 mM), and DON + IP6 (10 μM + 5 mM). DON elevated creatinine levels and γ-glutamyl transferase activity in the culture medium. It led to cytoplasmic vacuolation in convoluted tubular epithelial cells and tubular necrosis, a drop in antioxidant potential (ABTS) and a rise in interleukin (IL)-8 mRNA. The introduction of IP6 restored renal histological and functional parameters, bolstered antioxidant status, enhanced IL-10 gene expression, and reduced superoxide anion levels. These results indicate that within the model of renal explants, IP6 significantly alleviates the nephrotoxic effects of DON while efficiently regulating the antioxidative and anti-inflammatory response within the kidneys.
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Inositol-6-phosphate mitigates deoxynivalenol nephrotoxicity in pig kidney explants
In: World Mycotoxin JournalSearch for other papers by W.G. Suhett in
Current site
Google Scholar
PubMed
Search for other papers by J.R. Gerez in
Current site
Google Scholar
PubMed
Search for other papers by M.S. Hohmann in
Current site
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PubMed
Search for other papers by L. Staurengo-Ferrari in
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PubMed
Search for other papers by W.A. Verri in
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Search for other papers by F.H.O. Pinho in
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Search for other papers by L.D. de Barros in
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Abstract
Deoxynivalenol (DON) is a mycotoxin that frequently contaminates food crops and negatively impacts human and animal health. While inositol-6-phosphate (IP6) is known to counteract DON-induced intestinal damage, inflammation, and oxidative stress, its ability to alleviate DON-caused kidney injury is yet unexplored. This study assessed DON’s kidney effects and IP6’s protective capability using an ex vivo renal explants model. From 360 explants taken from six pigs, four groups were formed: control (culture medium), DON (10 μM equivalent to 3 mg/kg of feed), IP6 group (5 mM), and DON + IP6 (10 μM + 5 mM). DON elevated creatinine levels and γ-glutamyl transferase activity in the culture medium. It led to cytoplasmic vacuolation in convoluted tubular epithelial cells and tubular necrosis, a drop in antioxidant potential (ABTS) and a rise in interleukin (IL)-8 mRNA. The introduction of IP6 restored renal histological and functional parameters, bolstered antioxidant status, enhanced IL-10 gene expression, and reduced superoxide anion levels. These results indicate that within the model of renal explants, IP6 significantly alleviates the nephrotoxic effects of DON while efficiently regulating the antioxidative and anti-inflammatory response within the kidneys.
| All Time | Past 365 days | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 441 | 86 | 23 |
| Full Text Views | 17 | 3 | 1 |
| PDF Views & Downloads | 38 | 6 | 0 |
