Effects of dietary aflatoxin on biochemical parameters and histopathology of liver in Matrinxã (Brycon cephalus) and Pacu (Piaractus mesopotamicus) fish
In: World Mycotoxin JournalSearch for other papers by E. C. Michelin in
Current site
Google Scholar
Search for other papers by C. M. Bedoya-Serna in
Current site
Google Scholar
Search for other papers by L. C. S. Carrion in
Current site
Google Scholar
Search for other papers by N. Levy-Pereira in
Current site
Google Scholar
Search for other papers by F. S. Cury in
Current site
Google Scholar
Search for other papers by D. Passarelli in
Current site
Google Scholar
Search for other papers by C. G. Lima in
Current site
Google Scholar
Search for other papers by G. S. Yasui in
Current site
Google Scholar
Search for other papers by R. L. M. Sousa in
Current site
Google Scholar
Search for other papers by A. M. Fernandes in
Current site
Google Scholar
The objective of this study was to evaluate biochemical parameters and histopathology of liver in Matrinxã (Brycon cephalus) and Pacu (Piaractus mesopotamicus) fish chronically exposed to dietary aflatoxins. Fish feed was artificially contaminated with aflatoxins and the treatments were: Control â feed without toxin; Treatment A â feed + 10 μg aflatoxin B1 (AFB1)/kg; Treatment B â feed + 20 μg AFB1/kg; and Treatment C â feed + 50 μg AFB1/kg. Matrinxã and Pacu juvenile fish were placed in tanks for 180 days. Five experimental units per treatment were monthly sampled and submitted to blood collection and removal of hepatic tissue. Thus, twenty blood and liver samples for each species were collected monthly, adding up to 240 samples analysed. To verify biochemical changes, analyses included total proteins, albumin, globulins, aspartate aminotransferase (AST) and alkaline phosphatase (ALP). The hepatic tissue was examined microscopically and the slides presenting histopathological changes were photo-documented. There was effect of treatment (P<0.05) for AST and ALP in Matrinxã, while no effect (P>0.05) was observed in Pacu. A reduction (P<0.05) in AST and ALP values during the time of exposure was observed in all treatments for both species. Fatty degeneration and liver damage were observed for both species in treatments exposed to aflatoxins. Fatty degeneration in Pacu was noticed after 30 days of exposure, while in Matrinxã it was observed after 60 days. Disorganisation of the hepatocyte cord arrangement was also observed in those treatments exposed to aflatoxin, following 90 days of exposure in Matrinxã, and after 60 days in Pacu. Therefore, aflatoxins have little influence on biochemical parameters in the species evaluated. However, exposure to aflatoxins caused liver changes, such as cell death, fatty and hydropic degeneration, thus it could be concluded that both species are susceptible to the toxic effects of long-term exposure to dietary AFB1.
Purchase
Buy instant access (PDF download and unlimited online access):
Institutional Login
Log in with Open Athens, Shibboleth, or your institutional credentials
Personal login
Log in with your brill.com account
-
Abdelhamid, A.M., Abdel-Khalek, A.E., Mehrim, A.I. and Khalil, F.F., 2004. An attempt to alleviate aflatoxicosis on Nile tilapia fish by dietary supplementations with chicken-hatchery by-products (egg shells) and shrimp processing wastes (shrimp shells) 2 â On clinical, blood and histological parameters. Mansoura University Journal of Agricultural Sciences 29: 6175-6196.
'An attempt to alleviate aflatoxicosis on Nile tilapia fish by dietary supplementations with chicken-hatchery by-products (egg shells) and shrimp processing wastes (shrimp shells) 2 â On clinical, blood and histological parameters ' () 29 Mansoura University Journal of Agricultural Sciences : 6175 -6196.
-
Agência Nacional de Vigilância Sanitária (ANVISA), 2011. Resolução RDC no 7, de 18 de fevereiro de 2011. Dispõe sobre limites máximos tolerados (LMT) para micotoxinas em alimentos. Diário Oficial [da] República Federativa do Brasil. Available at:https://tinyurl.com/3zrp37c7.
-
Amany, M.K., Hala, M., Mohammad, M. and Abdel-Wahab, M., 2009. Pathological studies on effects of aflatoxin onOreochromis niloticus with application of different trials of control. Journal of Basic and Applied Zoology 22: 175-193. Available at:https://tinyurl.com/5j2662u9.
-
Atli, G., Ariyurek, S.Y., Kanak, E.G. and Canli, M., 2015. Alterations in the serum biomarkers belonging to different metabolic systems of fish (Oreochromis niloticus) after Cd and Pb exposures. Environmental Toxicology and Pharmacology 40: 508-515.https://doi.org/10.1016/j.etap.2015.08.001
-
Barni, S., Bernocchi, G. and Gerzeli, G., 1985. Morphohistochemical changes in hepatocytes during the life cycle of the European eel. Tissue and Cell 17: 97-109.https://doi.org/10.1016/0040-8166(85)90018-7
-
Batatinha, M.J.M., Simas, M.M.S. and Górniak, S.L., 2008. Micotoxicoses. In: Spinosa, H.S., Górniak, S. L. and Palermo Neto, J. (eds.) Toxicologia veterinária. Manole, São Paulo, Brazil, pp. 482-491.
'Micotoxicoses ', () 482 -491.
-
Bedoya-Serna, C.M., Michelin, E.C., Massocco, M.M., Carrion, L.C., Godoy, S.H., Lima, C.G., Ceccarelli, P.S., Yasui, G.S., Rottinghaus, G.E., Sousa, R.L.M. and Fernandes, A.M., 2019. Effects of dietary aflatoxin B1 on accumulation and performance in matrinxã fish (Brycon cephalus). PLoS ONE 13: e0201812.https://doi.org/10.1371/journal.pone.0201812
-
Bernet, D., Schmidt, H., Meier, W., Burkhardt-Holm, P. and Wahli, T., 1999. Histopathology in fish: proposal for a protocol to assess aquatic pollution. Journal of Fish Diseases 22: 25-34.https://doi.org/10.1046/j.1365-2761.1999.00134.x
-
Bintvihok, A. and Kositcharoenkul, S., 2006. Effect of dietary calcium propionate on performance, hepatic enzyme activities and aflatoxin residues in broilers fed a diet containing low levels of aflatoxin B1. Toxicon 47: 41-46.https://doi.org/10.1016/j.toxicon.2005.09.009
-
Boyd, C.E., 1990. Water quality in ponds for aquaculture. Alabama Agricultural Experiment Station, Auburn University, Auburn, AL, USA.
'Water quality in ponds for aquaculture', ().
-
Brasil, 1988. Ministério da Agricultura, Pecuária e Abastecimento â MAPA. Portaria MA/SNAD/SFA no 07 de novembro de 1988. Diário Oficial da União, BrasÃlia, DF, 09 nov. 1988. Seção I, p. 21.968.https://www.jusbrasil.com.br/diarios/DOU/1988/11/09
-
Brasil, 2005. Conselho Nacional do Meio Ambiente-Conama, Resolução no 357, de 17 de março de 2005. Diário Oficial da União, BrasÃlia, DF, 18 mar. 2005 p. 58-63.http://www2.mma.gov.br/port/conama/legiabre.cfm?codlegi=459
-
Cagauan, A.G., Tayaban, R.H., Somga, J. and Bartolome, R.M., 2004. Effect of aflatoxin-contaminated feeds in Nile tilapia (Oreochromis niloticus L.). In: Abstract of the 6th international symposium on tilapia in aquaculture (ISTA 6) section: health management and diseases Manila, Philippines, pp. 172-178. Available at:https://cals.arizona.edu/oip/ista6/ista6web/pdf/172.pdf.
-
Chavez-Sanchez, M.C., Palacios, C.M. and Moreno, I.O., 1994. Pathological effects of feeding youngOreochromis niloticus diets supplemented with different levels of aflatoxin B1. Aquaculture 127: 49-60.https://doi.org/10.1016/0044-8486(94)90191-0
-
Choi, K.C., Chung, W.T., Kwon, J.K., Yu, J.Y., Jang, Y.S., Park, S.M., Lee, S.Y. and Lee, J.C., 2010. Inhibitory effects of quercetin on aflatoxin B1-induced hepatic damage in mice. Food and Chemical Toxicology 48: 2747-2753.https://doi.org/10.1016/j.fct.2010.07.001
-
Cruz, Z. M.A., de Castro, R.R.L., Canal, A., Júnior, R.R.A., da Silva, A.G. and Ramos, A., 2013. Biochemical and cellular changes inOreochromis niloticus related to the water pollution of a degraded river. Acta Scientiarum â Biological Sciences 35: 419-427.https://doi.org/10.4025/actascibiolsci.v35i3.13207
-
Deng, S.X., Tian, L.X., Liu, F.J., Jin, S.J., Liang, G.Y., Yang, H.J. and Liu, Y.J., 2010. Toxic effects and residue of aflatoxin B1 in tilapia (Oreochromis niloticus ÃO. aureus) during long-term dietary exposure. Aquaculture 307: 233-240.https://doi.org/10.1016/j.aquaculture.2010.07.029
-
El-Sayed, Y.S. and Khalil, R.H., 2009. Toxicity, biochemical effects and residue of aflatoxin B1 in marine water-reared sea bass (Dicentrarchus labrax L.). Food and Chemical Toxicology 47: 1606-1609.https://doi.org/10.1016/j.fct.2009.04.008
-
Espada, Y., Domingo, M., Gomez, J. and Calvo, M.A., 1992. Pathological lesions following an experimental intoxication with aflatoxin B1 in broiler chickens. Research in Veterinary Science 53: 275-279.https://doi.org/10.1016/0034-5288(92)90127-N
-
European Commission (EC), 2006. Commission Regulation (EC) no 1881/2006, of 19 December 2006. Setting maximum levels for certain contaminants in foodstuffs. Official Journal of the European Union 364: 5-24.
-
Food and Agricultural Organization of the United Nations (FAO), 2016. The state of the world fisheries and aquaculture. Available at:http://www.fao.org/3/a-i5555e.pdf.
-
Food and Drug Administration (FDA), 1995. Section 555.400: Foods â adulteration with aflatoxins. Available at:https://tinyurl.com/598sz97v.
-
Gadelha, E.S. and Da Costa Araújo, J., 2013. Criação de Matrinxã em cativeiro. Pubvet 7: 312-419.
'Criação de Matrinxã em cativeiro ' () 7 Pubvet : 312 -419.
-
Gingerich, W.H., 1982. Hepatic toxicology of fishes, 1982. In: Weber, L.F. (ed.). Aquatic toxicology. Plenum Press, New York, NY, USA, pp. 55-105.https://doi.org/10.1002/tox.20225
-
Huang, Y., Han, D., Xiao, X., Zhu, X., Yang, Y., Jin, J., Chen, Y. and Xie, S., 2014. Effect of dietary aflatoxin B1 on growth, fecundity and tissue accumulation in gibel carp during the stage of gonad development. Aquaculture 428: 236-242.https://doi.org/10.1016/j.aquaculture.2014.03.010
-
Huang, Y., Han, D., Zhu, X., Yang, Y., Jin, J., Chen, Y. and Xie, S., 2011. Response and recovery of gibel carp from subchronic oral administration of aflatoxin B1. Aquaculture 319: 89-97.https://doi.org/10.1016/j.aquaculture.2011.06.024
-
Hussein, H.S. and Brasel, J.M., 2001. Toxicity, metabolism, and impact of mycotoxins on humans and animals. Toxicology 167: 101-134.https://doi.org/10.1016/S0300-483X(01)00471-1
-
Instituto Brasileiro de Geografia e EstatÃstica (IBGE), 2016. Produção da pecuária municipal 2015. Vol. 43. IBGE, Rio de Janeiro, RJ, Brazil. Available at:https://biblioteca.ibge.gov.br/visualizacao/periodicos/84/ppm_2015_v43_br.pdf
-
International Agency for Research on Cancer (IARC), 2002. Aflatoxins. In: Monograph on the evaluation of carcinogenic risks to humans. Vol. 82. Some traditional herbal medicines, some mycotoxins, naphthalene and styrene. IARC, Lyon, France, pp. 171-300.
-
Jantrarotai, W. and Lovell, R.T., 1990. Subchronic toxicity of dietary aflatoxin B1 to channel catfish. Journal of Aquatic Animal Health 2: 248-254.https://doi.org/10.1577/1548-8667(1990)002<0248:STODAB>2.3.CO;2
-
Jindal, N., Mahipal, S.K. and Mahajan, N.K., 1994. Toxicity of aflatoxin B1 in broiler chicks and its reduction by activated charcoal. Research in Veterinary Science 56: 37-40.https://doi.org/10.1016/0034-5288(94)90193-7.
-
Lopes, P.R.S., Radünz Neto, J., Mallmann, C.A., Lazzari, R., Pedron, F.D.A. and Veiverberg, C.A., 2005. Crescimento e alterações no fÃgado e na carcaça de alevinos de jundiá alimentados com dietas com aflatoxinas. Pesquisa Agropecuária Basileira 40: 1029-1034.https://doi.org/10.1590/S0100-204X2005001000012
-
Macedo-Viegas, E.M., Scorvo, C.M.D.F., Vidotti, R.M. and Secco, E.M., 2000. Efeito das classes de peso sobre a composição corporal e o rendimento de processamento de matrinxã (Brycon cephalus). Acta Scientiarum â Animal Sciences 22: 725-728.https://doi.org/10.4025/actascianimsci.v22i0.3138
-
Michelin, E.C., Massocco, M.M., Godoy, S.H.S., Baldin, J.C., Yasui, G.S., Lima, C.G., Rottinghaus, G.E., Sousa, R.L.M. and Fernandes, A.M., 2017. Carryover of aflatoxins from feed to lambari fish (Astyanax altiparanae) tissues. Food Additives and Contaminants Part A 34: 265-272.https://doi.org/10.1080/19440049.2016.1266097
-
Motta, V.T., 2000. BioquÃmica clÃnica: princÃpios e interpretações. Médica Massau, Porto Alegre, Brazil.
'BioquÃmica clÃnica: princÃpios e interpretações', ().
-
Netto, J.D.P.C., Boscolo, W.R., Feiden, A., Maluf, M.L.F., Freitas, J.M.A.D. and Simões, M. R., 2010. Formulação, análises microbiológicas, composição centesimal e aceitabilidade de empanados de jundiá (Rhamdia quelen), pacu (Piaractus mesopotamicus) e tilápia (Oreochromis niloticus). Revista do Instituto Adolfo Lutz 69: 181-187.http://periodicos.ses.sp.bvs.br/scielo.php?script=sci_arttext&pid=S0073-98552010000200006&lng=pt&nrm=iso=pt
-
Omar, W.A., Saleh, Y.S., and Marie, M.A., 2014. Integrating multiple fish biomarkers and risk assessment as indicators of metal pollution along the Red Sea coast of Hodeida, Yemen Republic. Ecotoxicology and Environmental Safety 110: 221-31.https://doi.org/10.1016/j.ecoenv.2014.09.004
-
Ãner, M., Atli, G. and Canli, M., 2008. Changes in serum biochemical parameters of freshwater fishOreochromis niloticus following prolonged metal (Ag, Cd, Cr, Cu, Zn) exposures. Environmental Toxicology and Chemistr, 27: 360-366.https://doi.org/10.1897/07-281R.1
-
Robbins, S.L., Cotran, R.S. and Kumar, V., 1989. Robbins pathologic basis of disease. 4. ed. W.B. Saunders Co., Philadelphia, PA, USA.
'Robbins pathologic basis of disease', ().
-
Roberts, R.J., 2002. Nutritional pathology. In: Halver, J.E. and Hardy, R.W. (eds.) Fish nutrition. Academic Press, London, UK, pp. 489-491.
'Nutritional pathology ', () 489 -491.
-
Sahoo, P.K. and Mukherjee, S.C., 2001. Immunosuppressive effects of aflatoxin B1 in Indian major carp (Labeo rohita). Comparative Immunology, Microbiology and Infectious Diseases 24: 143-149.https://doi.org/10.1016/S0147-9571(00)00017-5
-
Sahoo, P.K., Mukherjee, S.C., Nayak, S.K. and Dey, S., 2001. Acute and subchronic toxicity of aflatoxin B1 to rohu,Labeo rohita (Hamilton). Indian Journal of Experimental Biology 39: 453-458.
'Acute and subchronic toxicity of aflatoxin B1 to rohu,Labeo rohita (Hamilton) ' () 39 Indian Journal of Experimental Biology : 453 -458.
-
Santacroce, M.P., Conversano, M.C., Casalino, E., Lai, O., Zizzadoro, C., Centoducati, G. and Crescenzo, G., 2008. Aflatoxins in aquatic species: metabolism, toxicity and perspectives. Reviews in Fish Biology and Fisheries 18: 99-130.https://doi.org/10.1007/s11160-007-9064-8
-
Santos, V.B., Santos, R.S., Salomão, R.A.S. and Silva, R.M., 2012. Reprodução induzida de pacu (Piaractus mesopotamicus) com o uso de diferentes hormônios comerciais. Pesquisa e Tecnologia 9 (1).
'Reprodução induzida de pacu (Piaractus mesopotamicus) com o uso de diferentes hormônios comerciais ' () 9 Pesquisa e Tecnologia .
-
Sepahdari, A., Ebrahimzadeh, M.H., Sharifpour, I., Khosravi, A., Motalebi, A.A., Mohseni, M. Kakoolaki, S., Pourali, H.R. and Halajian, A., 2010. Effects of different dietary levels of AFB1 on survival rate and growth factors of Beluga (Huso huso). Iranian Journal of Fisheries Science 9: 141-150.https://doi.org/10.22092/IJFS.2018.114089
-
Silva, V.K., Ferreira, M.W. and Logato, P.V.R., 2007. Qualidade da água na Piscicultura. Universidade Federal de Lavras, Lavras, MG, Brazil.
'Qualidade da água na Piscicultura', ().
-
Souza, V.L., Lunardi, L.O., Vasques, L.H., Casaletti, L., Nakaghi, L.S.O. and Urbinati, E. C., 2001. Morphometric alterations in hepatocytes and ultrastructural distribution of liver glycogen in pacu (Piaractus mesopotamicus Holmberg, 1887) during food restriction and refeeding. Journal of Morphological Sciences 18: 15-20.
'Morphometric alterations in hepatocytes and ultrastructural distribution of liver glycogen in pacu (Piaractus mesopotamicus Holmberg, 1887) during food restriction and refeeding ' () 18 Journal of Morphological Sciences : 15 -20.
-
Tavares, L.H.S., 1995. Limnologia aplicada à aquicultura. Funep, Jaboticabal, Brazil.
'Limnologia aplicada à aquicultura', ().
-
Thrall, M.A., Weiser, G., Allison, R.W. and Campbell, T.W., 2007. Hematologia e bioquÃmica clÃnica veterinária. Editora Roca, Rio de Janeiro, RJ, Brazil.
'Hematologia e bioquÃmica clÃnica veterinária', ().
-
Treinen-Moslen, M., 2001. Toxic responses of the liver. In: Klaassen, C.D. (ed.). Casarette and Doull´s toxicology: the basic science of poisons. McGraw Hill, Columbus, OH, USA, pp. 471-489.
'Toxic responses of the liver ', () 471 -489.
-
Tuan, N.A., Grizzle, J.M., Lovell, R.T., Manning, B.B. and Rottinghaus, G.E., 2002. Growth and hepatic lesions of Nile tilapia (Oreochromis niloticus) fed diets containing aflatoxin B1. Aquaculture 212: 311-319.https://doi.org/10.1016/S0044-8486(02)00021-2
-
Wang, X., Wang, Y., Li, Y., Huang, M., Gao, Y., Xue, X., Zhang, H., Encarnação, P., Santos, G.A. and Gonçalves, R.A., 2016. Response of yellow catfish (Pelteobagrus fulvidraco) to different dietary concentrations of aflatoxin B1 and evaluation of an aflatoxin binder in offsetting its negative effects. Ciencias Marinas 42: 15-29.https://doi.org/10.7773/cm.v42i1.2595
-
Yildirim, M. Z., Benlı, A. Ã. K., Selvı, M., Ãzkul, A., Erkoç, F. and Koçak, O., 2006. Acute toxicity, behavioral changes, and histopathological effects of deltamethrin on tissues (gills, liver, brain, spleen, kidney, muscle, skin) of Nile tilapia (L.) fingerlings. Environmental Toxicology 21: 614-620.
'Acute toxicity, behavioral changes, and histopathological effects of deltamethrin on tissues (gills, liver, brain, spleen, kidney, muscle, skin) of Nile tilapia (L.) fingerlings ' () 21 Environmental Toxicology : 614 -620.
-
Zychowski, K.E., Hoffmann, A.R., Ly, H.J., Pohlenz, C., Buentello, A., Romoser, A., Gatlin, D.M. and Phillips, T.D., 2013. The effect of aflatoxin-B1 on red drum (Sciaenops ocellatus) and assessment of dietary supplementation of NovaSil for the prevention of aflatoxicosis. Toxins 5: 1555-1573.https://doi.org/10.3390/toxins5091555
| All Time | Past 365 days | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 361 | 121 | 10 |
| Full Text Views | 29 | 0 | 0 |
| PDF Views & Downloads | 22 | 0 | 0 |
Effects of dietary aflatoxin on biochemical parameters and histopathology of liver in Matrinxã (Brycon cephalus) and Pacu (Piaractus mesopotamicus) fish
In: World Mycotoxin JournalSearch for other papers by E. C. Michelin in
Current site
Google Scholar
PubMed
Search for other papers by C. M. Bedoya-Serna in
Current site
Google Scholar
PubMed
Search for other papers by L. C. S. Carrion in
Current site
Google Scholar
PubMed
Search for other papers by N. Levy-Pereira in
Current site
Google Scholar
PubMed
Search for other papers by F. S. Cury in
Current site
Google Scholar
PubMed
Search for other papers by D. Passarelli in
Current site
Google Scholar
PubMed
Search for other papers by C. G. Lima in
Current site
Google Scholar
PubMed
Search for other papers by G. S. Yasui in
Current site
Google Scholar
PubMed
Search for other papers by R. L. M. Sousa in
Current site
Google Scholar
PubMed
Search for other papers by A. M. Fernandes in
Current site
Google Scholar
PubMed
The objective of this study was to evaluate biochemical parameters and histopathology of liver in Matrinxã (Brycon cephalus) and Pacu (Piaractus mesopotamicus) fish chronically exposed to dietary aflatoxins. Fish feed was artificially contaminated with aflatoxins and the treatments were: Control â feed without toxin; Treatment A â feed + 10 μg aflatoxin B1 (AFB1)/kg; Treatment B â feed + 20 μg AFB1/kg; and Treatment C â feed + 50 μg AFB1/kg. Matrinxã and Pacu juvenile fish were placed in tanks for 180 days. Five experimental units per treatment were monthly sampled and submitted to blood collection and removal of hepatic tissue. Thus, twenty blood and liver samples for each species were collected monthly, adding up to 240 samples analysed. To verify biochemical changes, analyses included total proteins, albumin, globulins, aspartate aminotransferase (AST) and alkaline phosphatase (ALP). The hepatic tissue was examined microscopically and the slides presenting histopathological changes were photo-documented. There was effect of treatment (P<0.05) for AST and ALP in Matrinxã, while no effect (P>0.05) was observed in Pacu. A reduction (P<0.05) in AST and ALP values during the time of exposure was observed in all treatments for both species. Fatty degeneration and liver damage were observed for both species in treatments exposed to aflatoxins. Fatty degeneration in Pacu was noticed after 30 days of exposure, while in Matrinxã it was observed after 60 days. Disorganisation of the hepatocyte cord arrangement was also observed in those treatments exposed to aflatoxin, following 90 days of exposure in Matrinxã, and after 60 days in Pacu. Therefore, aflatoxins have little influence on biochemical parameters in the species evaluated. However, exposure to aflatoxins caused liver changes, such as cell death, fatty and hydropic degeneration, thus it could be concluded that both species are susceptible to the toxic effects of long-term exposure to dietary AFB1.
| All Time | Past 365 days | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 361 | 121 | 10 |
| Full Text Views | 29 | 0 | 0 |
| PDF Views & Downloads | 22 | 0 | 0 |
