Degradation of ochratoxin A by supernatant and ochratoxinase ofAspergillus niger W-35 isolated from cereals
In: World Mycotoxin JournalBeijing Laboratory for Food Quality and Safety, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China P.R.
Search for other papers by M. Zhao in
Current site
Google Scholar
Beijing Laboratory for Food Quality and Safety, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China P.R.
Search for other papers by X.Y. Wang in
Current site
Google Scholar
Beijing Laboratory for Food Quality and Safety, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China P.R.
Search for other papers by S.H. Xu in
Current site
Google Scholar
Beijing Laboratory for Food Quality and Safety, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China P.R.
Search for other papers by G.Q. Yuan in
Current site
Google Scholar
Search for other papers by X.J. Shi in
Current site
Google Scholar
Beijing Laboratory for Food Quality and Safety, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China P.R.
Search for other papers by Z.H. Liang in
Current site
Google Scholar
Ochratoxin A (OTA) is a mycotoxin produced byAspergillus spp. andPenicillium spp. and poses a threat to food safety. Biodegradation may be a promising strategy for reducing the OTA contamination in the future. In this study,Aspergillus niger strain W-35 was isolated from cereals and studied for its ability to degrade OTA. Results showed that the supernatant of W-35 could degrade OTA bothin vitro and in commercial feeds after incubation at 37 °C for 12 h by 78.0 and 37.0%, respectively. Ochratoxin α (OTα) was assayed as a degradation product by HPLC-FLD. Furthermore, an enzyme specific for OTA degradation (ochratoxinase, OTase) obtained from W-35 was successfully expressed inEscherichia coli BL21, and degraded OTA at a rate of 85.1% for 12 h. These results indicated that this OTA degradation is enzymatic and that the responsible enzyme is extracellular OTase. Reliable degradation of OTA has the potential for wide-ranging applications in the food and feed industries.
Purchase
Buy instant access (PDF download and unlimited online access):
Institutional Login
Log in with Open Athens, Shibboleth, or your institutional credentials
Personal login
Log in with your brill.com account
-
Abrunhosa, L., Ines, A., Rodrigues, A.I., Guimaraes, A., Pereira, V.L., Parpot, P., Mendes-Faia, A. and Venancio, A., 2014. Biodegradation of ochratoxin A byPediococcus parvulus isolated from Douro wines. International Journal of Food Microbiology 188: 45-52.
'Biodegradation of ochratoxin A byPediococcus parvulus isolated from Douro wines ' () 188 International Journal of Food Microbiology : 45 -52.
-
Abrunhosa, L., Paterson, R.R.M. and Venancio, A., 2010. Biodegradation of ochratoxin A for food and feed decontamination. Toxins 2: 1078-1099. DOI:https://doi.org/10.3390/toxins2051078
-
Abrunhosa, L., Serra, R. and Venancio, A., 2002. Biodegradation of ochratoxin A by fungi isolated from grapes. Journal of Agricultural and Food Chemistry 50: 7493-7496. DOI:https://doi.org/10.1021/jf025747i
-
Abrunhosa, L. and Venancio, A., 2007. Isolation and purification of an enzyme hydrolyzing ochratoxin A fromAspergillus niger. Biotechnology Letters 29: 1909-1914. DOI:https://doi.org/10.1007/s10529-007-9479-2
-
Alvito, P.C., Sizoo, E.A., Almeida, C.M.M. and Van Egmond, H.P., 2010. Occurrence of aflatoxins and ochratoxin A in baby foods in Portugal. Food Analytical Methods 3: 22-30.
'Occurrence of aflatoxins and ochratoxin A in baby foods in Portugal ' () 3 Food Analytical Methods : 22 -30.
-
Basappa, S.C. and Shantha, T., 1996. Methods for detoxification of aflatoxins in foods and feeds – a critical appraisal. Journal of Food Science and Technology 33: 95-107.
'Methods for detoxification of aflatoxins in foods and feeds – a critical appraisal ' () 33 Journal of Food Science and Technology : 95 -107.
-
Bejaoui, H., Mathieu, F., Taillandier, P. and Lebrihi, A., 2005. Conidia of black aspergilli as new biological adsorbents for ochratoxin A in grape juices and musts. Journal of Agricultural and Food Chemistry 53: 8224.
'Conidia of black aspergilli as new biological adsorbents for ochratoxin A in grape juices and musts ' () 53 Journal of Agricultural and Food Chemistry : 8224.
-
Bui-Klimke, T.R. and Wu, F., 2015. Ochratoxin A and human health risk: a review of the evidence. Critical Reviews in Food Science and Nutrition 55: 1860-1869. DOI:https://doi.org/10.1080/10408398.2012.724480
-
Chang, X., Wu, Z., Wu, S., Dai, Y. and Sun, C., 2015. Degradation of ochratoxin A byBacillus amyloliquefaciens ASAG1. Food Additives and Contaminants Part A 32: 564-571. DOI:https://10.1080/19440049.2014.991948
-
Chen, J., Hu, L.B., Zhou, W., Yan, S.H., Yang, J.D., Xue, Y.F. and Shi, Z.Q., 2010. Degradation of microcystin-LR and RR by aStenotrophomonas sp. strain EMS isolated from Lake Taihu, China. International Journal of Molecular Sciences 11: 896-911. DOI:https://doi.org/10.3390/ijms11030896
-
Chen, W., Li, C., Zhang, B., Zhou, Z., Shen, Y., Liao, X., Yang, J., Wang, Y., Li, X. and Li, Y., 2018. Advances in biodetoxification of ochratoxin A – a review of the past five decades. Frontiers in Microbiology 9: 1386. DOI:https://doi.org/10.3389/fmicb.2018.01386
-
Cho, S.M., Jeong, S.E., Lee, K.R., Sudhani, H.P.K., Kim, M., Hong, S.-Y. and Chung, S.H., 2016. Biodegradation of ochratoxin A byAspergillus tubingensis isolated from meju. Journal of Microbiology and Biotechnology 26: 1687-1695. DOI:https://doi.org/10.4014/jmb.1606.06016
-
Cho, W.K., Sohn, U. and Kwak, J.W., 2000. Production andin vitro refolding of a single-chain antibody specific for human plasma apolipoprotein A-I. Journal of Biotechnology 77: 169-178.
'Production andin vitro refolding of a single-chain antibody specific for human plasma apolipoprotein A-I ' () 77 Journal of Biotechnology : 169 -178.
-
Dobritzsch, D., Wang, H., Schneider, G. and Yu, S., 2014. Structural and functional characterization of ochratoxinase, a novel mycotoxin-degrading enzyme. Biochemical Journal 462: 441-452. DOI:https://doi.org/10.1042/bj20140382
-
Ge, F., Zhu, L., Aang, A., Song, P., Li, W., Tao, Y. and Du, G., 2018. Recent advances in enhanced enzyme activity, thermostability and secretion by N-glycosylation regulation in yeast. Biotechnology Letters 40: 847-854. DOI:https://doi.org/10.1007/s10529-018-2526-3
-
Hou, J., Osterlund, T., Liu, Z., Petranovic, D. and Nielsen, J., 2013. Heat shock response improves heterologous protein secretion inSaccharomyces cerevisiae. Applied Microbiology and Biotechnology 97: 3559-3568. DOI:https://doi.org/10.1007/s00253-012-4596-9
-
Hu, H.N., Jia, X., Wang, Y.P. and Liang, Z.H., 2018. Removal of ochratoxin A by a carboxypeptidase and peptides present in liquid cultures ofBacillus subtilis CW14. World Mycotoxin Journal 11: 559-570. DOI:https://doi.org/10.3920/wmj2017.2296
-
International Agency for Research on Cancer (IARC), 1993. Some naturally occurring substances: food items and constituents, heterocyclic aromatic amines and mycotoxins. IARC Monographs on the evaluation of carcinogenic risks to humans. Vol. 56. IARC, Lyon, France.
-
Ishikawa, Y., Rubin, K., Bachinger, H.P. and Kalamajski, S., 2018. The endoplasmic reticulum-resident collagen chaperone Hsp47 interacts with and promotes the secretion of decorin, fibromodulin, and lumican. Journal of Biological Chemistry 293: 13707-13716. DOI:https://doi.org/10.1074/jbc.RA117.000758
-
Jard, G., Liboz, T., Mathieu, F., Guyonvarc’h, A. and Lebrihi, A., 2009. Adsorption of zearalenone byAspergillus japonicus conidia: new trends for biological decontamination in animal feed. World Mycotoxin Journal 2: 391-397. DOI:https://doi.org/10.3920/wmj2008.1128
-
Kumar, N.S.M., Kishore, V. and Manonmani, H.K., 2014. Chemical modification of L-asparaginase fromCladosporium sp for improved activity and thermal stability. Preparative Biochemistry and Biotechnology 44: 433-450. DOI:https://doi.org/10.1080/10826068.2013.833110
-
Laemmli, U.K., 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685.
'Cleavage of structural proteins during the assembly of the head of bacteriophage T4 ' () 227 Nature : 680 -685.
-
Lee, H.J. and Ryu, D., 2017. Worldwide occurrence of mycotoxins in cereals and cereal derived food products: public health perspectives of their co-occurrence. Journal of Agricultural and Food Chemistry 65: 7034-7051.
'Worldwide occurrence of mycotoxins in cereals and cereal derived food products: public health perspectives of their co-occurrence ' () 65 Journal of Agricultural and Food Chemistry : 7034 -7051.
-
Li, L., Yang, J. and Yuan, X., 2005. Study on synthesis of isocoumarins and its evolution. Journal of Nanjing Normal University (Science and Technology) 2005 (2). Available at:https://tinyurl.com/y2ahkzsa.
-
Loi, M., Fanelli, F., Liuzzi, V.C., Logrieco, A.F. and Mule, G., 2017. Mycotoxin biotransformation by native and commercial enzymes: present and future perspectives. Toxins 9: 111. DOI:https://doi.org/10.3390/toxins9040111
-
Luz, C., Ferrer, J., Mañes, J. and Meca, G., 2017. Toxicity reduction of ochratoxin A by lactic acid bacteria. Food and Chemical Toxicology 112: 60-66.
'Toxicity reduction of ochratoxin A by lactic acid bacteria ' () 112 Food and Chemical Toxicology : 60 -66.
-
Solfrizzo, M., Piemontese, L., Gambacorta, L., Zivoli, R. and Longobardi, F., 2015. Food coloring agents and plant food supplements derived fromVitis vinifera: a new source of human exposure to ochratoxin A. Journal of Agricultural and Food Chemistry 63: 3609-3614. DOI:https://doi.org/10.1021/acs.jafc.5b00326
-
Mobashar, M., Hummel, J., Blank, R. and Suedekum, K.-H., 2010. Ochratoxin A in ruminants – a review on its degradation by gut microbes and effects on animals. Toxins 2: 809-839. DOI:https://doi.org/10.3390/toxins204809
-
Niu, C., Zhu, L., Xu, X. and Li, Q., 2016. Rational design of disulfide bonds increases thermostability of a mesophilic 1,3-1,4-beta-glucanase fromBacillus terquilensis. PLoS ONE 11: e0154036. DOI:https://doi.org/10.1371/journal.pone.0154036
-
Palmira, D.B., Mariana, T., Miriam, H., Francesca, F., Angelo, S., Giuseppina, M. and Francesco, G., 2015. Biodegradation of ochratoxin A by bacterial strains isolated from vineyard soils. Toxins 7: 5079-5093.
'Biodegradation of ochratoxin A by bacterial strains isolated from vineyard soils ' () 7 Toxins : 5079 -5093.
-
Peteri, Z., Teren, J., Vagvolgyi, C. and Varga, J., 2007. Ochratoxin degradation and adsorption caused by astaxanthin-producing yeasts. Food Microbiology 24: 205-210. DOI:https://doi.org/10.1016/j.fm.2006.06.003
-
Petruzzi, L., Bevilacqua, A., Baiano, A., Beneduce, L., Corbo, M.R. and Sinigaglia, M., 2014.In vitro removal of ochratoxin A by two strains ofSaccharomyces cerevisiae and their performances under fermentative and stressing conditions. Journal of Applied Microbiology 116: 60-70. DOI:https://doi.org/10.1111/jam.12350
-
Pitout, M.J., 1969. Hydrolysis of ochratoxin A by some proteolytic enzymes. Biochemical Pharmacology 18: 485-491. DOI:https://doi.org/10.1016/0006-2952(69)90224-x
-
Quintela, S., Villaran, C.M., Armentia, L.I. and Elejalde, E., 2013. Ochratoxin A removal in wine: a review. Food Control 30: 439-445.
'Ochratoxin A removal in wine: a review ' () 30 Food Control : 439 -445.
-
Rodriguez, H., Reveron, I., Doria, F., Costantini, A., De las Rivas, B., Munoz, R. and Garcia-Moruno, E., 2011. Degradation of ochratoxin A byBrevibacterium species. Journal of Agricultural and Food Chemistry 59: 10755-10760. DOI:https://doi.org/10.1021/jf203061p
-
Shen, X.L., Zhang, B., Liang, R., Cheng, W.-H., Xu, W., Luo, Y., Zhao, C. and Huang, K., 2014. Central role of Nix in the autophagic response to ochratoxin A. Food and Chemical Toxicology 69: 202-209. DOI:https://doi.org/10.1016/j.fct.2014.04.017
-
Shen, X.L., Zhang, Y., Xu, W., Liang, R., Zheng, J., Luo, Y., Wang, Y. and Huang, K., 2013. An iTRAQ-based mitoproteomics approach for profiling the nephrotoxicity mechanisms of ochratoxin A in HEK 293 cells. Journal of Proteomics 78: 398-415. DOI:https://doi.org/10.1016/j.jprot.2012.10.010
-
Shi, L., Liang, Z., Li, J., Hao, J., Xu, Y., Huang, K., Tian, J., He, X. and Xu, W., 2014. Ochratoxin A biocontrol and biodegradation byBacillus subtilis CW 14. Journal of the Science of Food and Agriculture 94: 1879-1885. DOI:https://doi.org/10.1002/jsfa.6507
-
Shukla, S., Park, J.H., Chung, S.H. and Kim, M., 2018. Ochratoxin A reduction ability of biocontrol agentBacillus subtilis isolated from Korean traditional fermented food Kimchi. Scientific Reports 8: 8039.
'Ochratoxin A reduction ability of biocontrol agentBacillus subtilis isolated from Korean traditional fermented food Kimchi ' () 8 Scientific Reports : 8039.
-
Siahmoshteh, F., Siciliano, I., Banani, H., Hamidi-Esfahani, Z., Razzaghi-Abyaneh, M., Gullino, M.L. and Spadaro, D., 2017. Efficacy ofBacillus subtilis andBacillus amyloliquefaciens in the control ofAspergillus parasiticus growth and aflatoxins production on pistachio. International Journal of Food Microbiology 254: 47-53. DOI:https://doi.org/10.1016/j.ijfoodmicro.2017.05.011
-
Stander, M.A., Bornscheuer, U.T., Henke, E. and Steyn, P.S., 2000. Screening of commercial hydrolases for the degradation of ochratoxin A. Journal of Agricultural and Food Chemistry 48: 5736-5739.
'Screening of commercial hydrolases for the degradation of ochratoxin A ' () 48 Journal of Agricultural and Food Chemistry : 5736 -5739.
-
Varga, J., Rigó, K. and Téren, J., 2000. Degradation of ochratoxin A byAspergillus species. International Journal of Food Microbiology 59: 1-7.
'Degradation of ochratoxin A byAspergillus species ' () 59 International Journal of Food Microbiology : 1 -7.
-
Xiong, K., Wang, X.-L., Zhi, H.-W., Sun, B.-G. and Li, X.-T., 2017. Identification and safety evaluation of a product from the biodegradation of ochratoxin A by anAspergillus strain. Journal of the Science of Food and Agriculture 97: 434-443. DOI:https://doi.org/10.1002/jsfa.7742
-
Yang, Q., Wang, J., Zhang, H., Li, C. and Zhang, X., 2016. Ochratoxin A is degraded byYarrowia lipolytica and generates non-toxic degradation products. World Mycotoxin Journal 9: 269-278. DOI:https://doi.org/10.3920/wmj2015.1911
-
Zhang, B., Shen, X.L., Liang, R., Li, Y., Huang, K., Zhao, C., Luo, Y. and Xu, W., 2014. Protective role of the mitochondrial Lon protease 1 in ochratoxin A-induced cytotoxicity in HEK293 cells. Journal of Proteomics 101: 154-168. DOI:https://doi.org/10.1016/j.jprot.2014.02.017
-
Zhang, H.H., Wang, Y., Zhao, C., Wang, J. and Zhang, X.L., 2017. Biodegradation of ochratoxin A byAlcaligenes faecalis isolated from soil. Journal of Applied Microbiology 123: 661-668.
'Biodegradation of ochratoxin A byAlcaligenes faecalis isolated from soil ' () 123 Journal of Applied Microbiology : 661 -668.
-
Zhang, L.Z., Ni, H., Zhu, Y.B., Yang, Y.F., Li, L.J., Jiang, Z.D., Zheng, F.P. and Chen, F., 2018. Characterization of aromas of instant oolong tea and its counterparts treated with two crude enzymes fromAspergillus niger. Journal of Food Processing and Preservation 42: e13500. DOI:https://doi.org/10.1111/jfpp.13500
-
Zhao, T., Shen, X.L., Chen, W., Liao, X., Yang, J., Wang, Y., Zou, Y. and Fang, C., 2017. Advances in research of nephrotoxicity and toxic antagonism of ochratoxin A. Toxin Reviews 36: 39-44. DOI:https://doi.org/10.1080/15569543.2016.1243560
-
Zhong, Q.D., Li, G.H., Wang, D.B., Shao, Y., Li, J.G., Xiong, Z.H. and Wu, Y.N., 2014. Exposure assessment to ochratoxin A in Chinese wine. Journal of Agricultural and Food Chemistry 62: 8908-8913. DOI:https://doi.org/10.1021/jf500713x
| All Time | Past 365 days | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 504 | 157 | 26 |
| Full Text Views | 30 | 0 | 0 |
| PDF Views & Downloads | 22 | 1 | 0 |
Degradation of ochratoxin A by supernatant and ochratoxinase ofAspergillus niger W-35 isolated from cereals
In: World Mycotoxin JournalBeijing Laboratory for Food Quality and Safety, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China P.R.
Search for other papers by M. Zhao in
Current site
Google Scholar
PubMed
Beijing Laboratory for Food Quality and Safety, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China P.R.
Search for other papers by X.Y. Wang in
Current site
Google Scholar
PubMed
Beijing Laboratory for Food Quality and Safety, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China P.R.
Search for other papers by S.H. Xu in
Current site
Google Scholar
PubMed
Beijing Laboratory for Food Quality and Safety, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China P.R.
Search for other papers by G.Q. Yuan in
Current site
Google Scholar
PubMed
Search for other papers by X.J. Shi in
Current site
Google Scholar
PubMed
Beijing Laboratory for Food Quality and Safety, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China P.R.
Search for other papers by Z.H. Liang in
Current site
Google Scholar
PubMed
Ochratoxin A (OTA) is a mycotoxin produced byAspergillus spp. andPenicillium spp. and poses a threat to food safety. Biodegradation may be a promising strategy for reducing the OTA contamination in the future. In this study,Aspergillus niger strain W-35 was isolated from cereals and studied for its ability to degrade OTA. Results showed that the supernatant of W-35 could degrade OTA bothin vitro and in commercial feeds after incubation at 37 °C for 12 h by 78.0 and 37.0%, respectively. Ochratoxin α (OTα) was assayed as a degradation product by HPLC-FLD. Furthermore, an enzyme specific for OTA degradation (ochratoxinase, OTase) obtained from W-35 was successfully expressed inEscherichia coli BL21, and degraded OTA at a rate of 85.1% for 12 h. These results indicated that this OTA degradation is enzymatic and that the responsible enzyme is extracellular OTase. Reliable degradation of OTA has the potential for wide-ranging applications in the food and feed industries.
| All Time | Past 365 days | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 504 | 157 | 26 |
| Full Text Views | 30 | 0 | 0 |
| PDF Views & Downloads | 22 | 1 | 0 |
