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Inhibitory effect ofEnterobacter cloacae 3J1EC onAspergillus flavus 3.4408 growth and aflatoxin production

in World Mycotoxin Journal
Autor:innen:
X. Dong Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, China P.R.
Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, Wuhan 430062, China P.R.
National Reference Laboratory for Agricultural Testing (Biotoxin), Wuhan 430062, China P.R.

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Q. Zhang Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, China P.R.
Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, Wuhan 430062, China P.R.
Laboratory of Quality & Safety Risk Assessment for Oilseeds Products, Wuhan, Ministry of Agriculture and Rural Affairs, Wuhan 430062, China P.R.

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Z. Zhang Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, China P.R.
National Reference Laboratory for Agricultural Testing (Biotoxin), Wuhan 430062, China P.R.
Key Laboratory of Detection for Mycotoxins, Ministry of Agriculture and Rural Affairs, Wuhan 430062, China P.R.

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X. Yue Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, China P.R.
Quality Inspection and Test Center for Oilseeds Products, Ministry of Agriculture, Wuhan 430062, China P.R.

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L. Zhang Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, China P.R.
Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, Wuhan 430062, China P.R.
Quality Inspection and Test Center for Oilseeds Products, Ministry of Agriculture, Wuhan 430062, China P.R.

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X. Chen Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, China P.R.

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W. Zhang Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, China P.R.
Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, Wuhan 430062, China P.R.
Quality Inspection and Test Center for Oilseeds Products, Ministry of Agriculture, Wuhan 430062, China P.R.

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L. Chen Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, China P.R.

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P. Li Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, China P.R.
Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, Wuhan 430062, China P.R.
National Reference Laboratory for Agricultural Testing (Biotoxin), Wuhan 430062, China P.R.
Laboratory of Quality & Safety Risk Assessment for Oilseeds Products, Wuhan, Ministry of Agriculture and Rural Affairs, Wuhan 430062, China P.R.
Key Laboratory of Detection for Mycotoxins, Ministry of Agriculture and Rural Affairs, Wuhan 430062, China P.R.
Quality Inspection and Test Center for Oilseeds Products, Ministry of Agriculture, Wuhan 430062, China P.R.

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Aspergillus flavus can easily infect major agricultural products and produce aflatoxin. In this study, we investigated the effect of the biocontrol bacteriumEnterobacter cloacae 3J1EC on the growth ofA. flavus strain 3.4408. The biocontrol bacterium played a key role in preventing infection byA. flavus. E. cloacae 3J1EC was found to inhibit the growth ofA. flavus 3.4408 mycelial pellets and reduce the production of aflatoxin by 96.9%. We found differential expression between the control and the treatment groups in the transcriptome ofA. flavus 3.4408. Gene ontology (GO) analysis indicated thatE. cloacae 3J1EC induced the down-regulated expression of cellular component and molecular function, while its effects on the up-regulated expression indicated the relationship of biological process and molecular function. Thus, these results suggest thatE. cloacae 3J1EC decreased aflatoxin production via down-regulated gene expression in terms of aflatoxin biosynthesis. In summary,E. cloacae 3J1EC can be employed as an alternative for the biological control ofA. flavus 3.4408.

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