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Challenge with fumonisins B1 and B2 changes IGF-1 and GHR mRNA expression in liver of Nile tilapia fingerlings

In: World Mycotoxin Journal
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S.C. Claudino-Silva Department of Veterinary Science, University Center of Maringá, Av. Guedner 1610, Maringá, PR 87050-390, Brazil.

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B. Lala Department of Animal Science, São Paulo State University, College of Veterinary Medicine and Animal Sciences (campus Botucatu), P.O. Box 560, Botucatu, SP 18618-970, Brazil.

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N.H.A.P. Mora Department of Animal Science, Faculdades Unidas do Vale do Araguaia, Rua Moreira Cabral 1000, Barra do Garças, MT 78600-000, Brazil.

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C.R. Schamber Department of Pharmacology and Therapeutics, State University of Maringá, Av. Colombo 5.790, Maringá, PR 87020-900, Brazil.

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C.S. Nascimento Department of Animal Science, Federal University of Sergipe, Av. Marechal Rondon s/n, São Cristóvão, SE 49100-000, Brazil.

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V.V. Pereira Department of Engineering, Faculdade Pitágoras, Rua Santos Dumont 1001, Divinópolis, MG 35500-286, Brazil.

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D.L. Hedler Department of Veterinary Science, University Center of Maringá, Av. Guedner 1610, Maringá, PR 87050-390, Brazil.

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E. Gasparino Department of Animal Science, State University of Maringá, Av. Colombo, 5790, Maringá, PR 87020-900, Brazil.

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Although fumonisins are identified as responsible for alterations in weight gain, little information is available on their effects on expression of growth-related genes, especially for Nile tilapia (Oreochromis niloticus) fingerlings. In this study, Nile tilapia fingerlings were treated with increasing levels of fumonisin B1 (FB1) and fumonisin B2 (FB2) (diets of 0, 20, 40, and 60 mg/kg) to evaluate their effects on weight gain (WG), feed intake (FI), feed efficiency (FE), growth hormone receptor (GHR) and insulin growth factor 1 (IGF-1) mRNA expression in liver of this fish. All variables were evaluated at 15 and 30 days of treatment. Diet containing 0 mg fumonisin/kg was used as control treatment. Treatment with 20, 40, and 60 mg fumonisin/kg of diet significantly reduced WG (P<0.0001) and FE (P<0.0001), while GHR and IGF-1 mRNA expression was reduced both at 15 and 30 days of treatment. Feed intake was not affected by diets in any of the evaluated periods. These results indicate that fumonisins (FB1 + FB2) affect the growth of Nile tilapia fingerlings through mechanisms that involve reduction of GHR and IGF-1 expression.

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