Simultaneous analysis of multiple mycotoxins inAlpinia oxyphylla by UPLC-MS/MS
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SCIEX Asia Pacific Application Support Center, Beijing 100015, China P.R.
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Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, 151 Ma Lian Wa North Road, Beijing 100193, China P.R.
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Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, 151 Ma Lian Wa North Road, Beijing 100193, China P.R.
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In this study, a reliable and fast method for the simultaneous quantitation of 11 mycotoxins inAlpinia oxyphylla was developed using ultra-performance liquid chromatography coupled with tandem mass spectrometry (UPLCMS/ MS). Three different extraction procedures (solid-liquid extraction, solid-phase extraction and modified QuEChERS) were evaluated. Solid-liquid extraction was fast and easy, and also provided the best recovery rate for all mycotoxins, compared to the other extraction procedures. Some crucial factors, including extraction solvent, time and temperature, were carefully optimised. Significant matrix effects were offset using matrix-matched calibration. Under these optimised conditions, our detection approach showed a good, linear dynamic range with correlation coefficients (R 2) above 0.9958. The limit of quantification ranged from 0.1 to 20 μg/kg. Accuracy was determined in a selected matrix using blank samples spiked with the target mycotoxins at three different concentration levels. The recoveries ranged from 60% (T-2 toxin) to 111% (HT-2 toxin), with relative standard deviation <20%. The validated method was used to analyse 44 batches ofA. oxyphylla purchased from different regions of China. Aflatoxin B1, zearalenone, ochratoxin A, fumonisin B1 and fumonisin B2 were detected in 4 mouldy samples.
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Supplementary Materials
| å ¨é¨æé´ | è¿å»ä¸å¹´ | è¿å»30天 | |
|---|---|---|---|
| æè¦æµè§æ¬¡æ° | 422 | 99 | 33 |
| å ¨ææµè§æ¬¡æ° | 27 | 0 | 0 |
| PDFä¸è½½æ¬¡æ° | 13 | 0 | 0 |
Simultaneous analysis of multiple mycotoxins inAlpinia oxyphylla by UPLC-MS/MS
äºWorld Mycotoxin JournalSearch for other papers by X.S. Zhao in
Current site
Google Scholar
PubMed
Search for other papers by W.J. Kong in
Current site
Google Scholar
PubMed
SCIEX Asia Pacific Application Support Center, Beijing 100015, China P.R.
Search for other papers by S. Wang in
Current site
Google Scholar
PubMed
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, 151 Ma Lian Wa North Road, Beijing 100193, China P.R.
Search for other papers by J.H. Wei in
Current site
Google Scholar
PubMed
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, 151 Ma Lian Wa North Road, Beijing 100193, China P.R.
Search for other papers by M.H. Yang in
Current site
Google Scholar
PubMed
In this study, a reliable and fast method for the simultaneous quantitation of 11 mycotoxins inAlpinia oxyphylla was developed using ultra-performance liquid chromatography coupled with tandem mass spectrometry (UPLCMS/ MS). Three different extraction procedures (solid-liquid extraction, solid-phase extraction and modified QuEChERS) were evaluated. Solid-liquid extraction was fast and easy, and also provided the best recovery rate for all mycotoxins, compared to the other extraction procedures. Some crucial factors, including extraction solvent, time and temperature, were carefully optimised. Significant matrix effects were offset using matrix-matched calibration. Under these optimised conditions, our detection approach showed a good, linear dynamic range with correlation coefficients (R 2) above 0.9958. The limit of quantification ranged from 0.1 to 20 μg/kg. Accuracy was determined in a selected matrix using blank samples spiked with the target mycotoxins at three different concentration levels. The recoveries ranged from 60% (T-2 toxin) to 111% (HT-2 toxin), with relative standard deviation <20%. The validated method was used to analyse 44 batches ofA. oxyphylla purchased from different regions of China. Aflatoxin B1, zearalenone, ochratoxin A, fumonisin B1 and fumonisin B2 were detected in 4 mouldy samples.
| å ¨é¨æé´ | è¿å»ä¸å¹´ | è¿å»30天 | |
|---|---|---|---|
| æè¦æµè§æ¬¡æ° | 422 | 99 | 33 |
| å ¨ææµè§æ¬¡æ° | 27 | 0 | 0 |
| PDFä¸è½½æ¬¡æ° | 13 | 0 | 0 |
