Deoxynivalenol and nivalenol analysis in soybean and soy flour
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A rapid and accurate method of quantifying deoxynivalenol (DON) and nivalenol (NIV) in soybean and soy flour is described. The samples were extracted with acetonitrile:water (84:16, v/v) and cleaned through a solidphase extraction (SPE) column. The mycotoxins were separated, detected and quantified by reversed-phase high performance liquid chromatography (HPLC) with UV detection (220 nm) using water:methanol (88:12, v/v) as mobile phase. Characteristics of this in-house method such as accuracy, precision and detection and quantification limits were defined by means of a recovery test with spiked soybean and soy flour samples. The detection limit (LOD) was 0.1 µg/g for DON and 0.2 µg/g for NIV, based on a signal-noise ratio 3:1. Quantification limit (LOQ) was established as three times the detection limit.
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| å ¨é¨æé´ | è¿å»ä¸å¹´ | è¿å»30天 | |
|---|---|---|---|
| æè¦æµè§æ¬¡æ° | 213 | 103 | 21 |
| å ¨ææµè§æ¬¡æ° | 22 | 1 | 0 |
| PDFä¸è½½æ¬¡æ° | 16 | 0 | 0 |
Deoxynivalenol and nivalenol analysis in soybean and soy flour
äºWorld Mycotoxin JournalSearch for other papers by G. Barros in
Current site
Google Scholar
PubMed
Search for other papers by D. GarcÃa in
Current site
Google Scholar
PubMed
Search for other papers by S. Oviedo in
Current site
Google Scholar
PubMed
Search for other papers by M. Ramirez in
Current site
Google Scholar
PubMed
Search for other papers by A. Torres in
Current site
Google Scholar
PubMed
Search for other papers by S. Chulze in
Current site
Google Scholar
PubMed
A rapid and accurate method of quantifying deoxynivalenol (DON) and nivalenol (NIV) in soybean and soy flour is described. The samples were extracted with acetonitrile:water (84:16, v/v) and cleaned through a solidphase extraction (SPE) column. The mycotoxins were separated, detected and quantified by reversed-phase high performance liquid chromatography (HPLC) with UV detection (220 nm) using water:methanol (88:12, v/v) as mobile phase. Characteristics of this in-house method such as accuracy, precision and detection and quantification limits were defined by means of a recovery test with spiked soybean and soy flour samples. The detection limit (LOD) was 0.1 µg/g for DON and 0.2 µg/g for NIV, based on a signal-noise ratio 3:1. Quantification limit (LOQ) was established as three times the detection limit.
| å ¨é¨æé´ | è¿å»ä¸å¹´ | è¿å»30天 | |
|---|---|---|---|
| æè¦æµè§æ¬¡æ° | 213 | 103 | 21 |
| å ¨ææµè§æ¬¡æ° | 22 | 1 | 0 |
| PDFä¸è½½æ¬¡æ° | 16 | 0 | 0 |
