Rapid LC and LC/MS for routine analysis of mycotoxins in foods
äºWorld Mycotoxin JournalSearch for other papers by H. Åenyuva in
Current site
Google Scholar
Search for other papers by J. Gilbert in
Current site
Google Scholar
Search for other papers by S. Ãzcan in
Current site
Google Scholar
Search for other papers by N. Gürel in
Current site
Google Scholar
Affinity column clean-up of food samples for mycotoxin analysis produces extracts which are free of co-extractives and therefore require little chromatography for separation and quantification of the target analytes. Using such clean extracts, we report rapid chromatographic methods for aflatoxins B1, B2, G1 and G2, aflatoxin M1, ochratoxin A, zearalenone and fumonisins. Using short columns with small particle size packing, HPLC conditions have been developed reducing analysis time typically by 75%, e.g. for aflatoxins from 19.0 min to 4.0 min with full separation of the four toxins and for aflatoxin M1 giving an analysis time of less than 1 min compared to 5 min for conventional analysis. Fumonisins were analysed directly by LC/MS in a run time of 4.1 min, using selected ion monitoring to avoid the need for derivatisation for fluorescence detection by HPLC. Peak purity under rapid analysis conditions has been demonstrated by LC/MS for a variety of food extracts such as pistachios, hazelnuts, paprika, cocoa, coffee, cereals, sesame oil, cheese, maize, animal feed, dried figs, dried vine fruits, cornflakes and bread. These substantial reductions in analysis time offer significant benefits to laboratories undertaking routine screening and quantitative analysis of mycotoxins in foods.
Purchase
Buy instant access (PDF download and unlimited online access):
Institutional Login
Log in with Open Athens, Shibboleth, or your institutional credentials
Personal login
Log in with your brill.com account
-
Berthiller, F., Schuhmacher, R., Buttinger, G. and Krska, R., 2005. Rapid simultaneous determination of major type A- and Btrichothecenes as well as zearalenone in maize by high performance liquid chromatographyâtandem mass spectrometry. Journal of Chromatography A 1062: 209-216.
'Rapid simultaneous determination of major type A- and Btrichothecenes as well as zearalenone in maize by high performance liquid chromatographyâtandem mass spectrometry ' () 1062 Journal of Chromatography A : 209 -216 .
-
Dragacci, S., Grosso, F. and Gilbert, J., 2001. Immunoaffinity column cleanup with liquid chromatography for determination of Aflatoxin M1 in liquid milk: Collaborative Study. Journal of the Association of Official Analytical Chemists International 84: 437-443.
'Immunoaffinity column cleanup with liquid chromatography for determination of Aflatoxin M1 in liquid milk: Collaborative Study ' () 84 Journal of the Association of Official Analytical Chemists International : 437 -443 .
-
Entwisle, A.C., Williams, A.C., Mann, P.J., Slack, P.T. and Gilbert, J., 2000. Liquid chromatographic method with immunoaffinity column cleanup for determination of Ochratoxin A in barley. Journal of the Association of Official Analytical Chemists International 83: 1377-1383.
'Liquid chromatographic method with immunoaffinity column cleanup for determination of Ochratoxin A in barley ' () 83 Journal of the Association of Official Analytical Chemists International : 1377 -1383 .
-
Kirkland, J.J., 2000. Ultrafast reversed phase high-performance liquid chromatographic separations: An overview. Journal of Chromatographic Science 38: 535-544.
'Ultrafast reversed phase high-performance liquid chromatographic separations: An overview ' () 38 Journal of Chromatographic Science : 535 -544 .
-
Macdonald, S.J., Anderson, S., Brereton, P., Wood, R. and Damant, A., 2005. Determination of zearlalenone in barley, maize and wheat flour, polenta and maize-based baby food by immunoaffinity column cleanup with liquid chromatography: Interlaboratory Study. Journal of the Association of Official Analytical Chemists International 88: 1733-1739.
'Determination of zearlalenone in barley, maize and wheat flour, polenta and maize-based baby food by immunoaffinity column cleanup with liquid chromatography: Interlaboratory Study ' () 88 Journal of the Association of Official Analytical Chemists International : 1733 -1739 .
-
Martin, M. and Guiochon, G., 2005. Effects of high pressure in liquid chromatography. Journal of Chromatography A 1090: 16-38.
'Effects of high pressure in liquid chromatography ' () 1090 Journal of Chromatography A : 16 -38 .
-
Ãzcan, S. and Åenyuva, H.Z., 2006. Improved and simplified liquid chromatography/atmospheric pressure chemical ionization mass spectrometry method for the analysis of underivatized free amino acids in various foods. Journal of Chromatography A 1135: 179-185.
'Improved and simplified liquid chromatography/atmospheric pressure chemical ionization mass spectrometry method for the analysis of underivatized free amino acids in various foods ' () 1135 Journal of Chromatography A : 179 -185 .
-
Ren, Y., Zhang, Yu., Shao, S., Cai, Z., Feng, I., Pan, H. and Wang, Z., 2007. Simultaneous determination of multi-component mycotoxin contaminants in foods and feeds by ultra-performance liquid chromatography tandem mass spectrometry. Journal of Chromatography A 1143: 48-64.
'Simultaneous determination of multi-component mycotoxin contaminants in foods and feeds by ultra-performance liquid chromatography tandem mass spectrometry ' () 1143 Journal of Chromatography A : 48 -64 .
-
Rundberget, T. and Wilkins, A.L., 2002. Determination of Penicillium mycotoxins in foods and feeds using liquid chromatographyâmass spectrometry. Journal of Chromatography A 964:189-197.
'Determination of Penicillium mycotoxins in foods and feeds using liquid chromatographyâmass spectrometry ' () 964 Journal of Chromatography A : 189 -197 .
-
Scott, P.M. and Trucksess, M.W., 1997. Application of immunoaffinity columns to mycotoxin analysis. Journal of the Association of Official Analytical Chemists International 80: 941-949.
'Application of immunoaffinity columns to mycotoxin analysis ' () 80 Journal of the Association of Official Analytical Chemists International : 941 -949 .
-
Åenyuva, H.Z. and Gilbert, J., 2005. Immunoaffinity column cleanup with liquid chromatography using post-column bromination for determination of Aflatoxins in hazelnut paste: Interlaboratory Study. Journal of the Association of Official Analytical Chemists International 88: 526-535.
'Immunoaffinity column cleanup with liquid chromatography using post-column bromination for determination of Aflatoxins in hazelnut paste: Interlaboratory Study ' () 88 Journal of the Association of Official Analytical Chemists International : 526 -535 .
-
Åenyuva, H.Z., Gilbert, J. and Ulken, U., 2007. Aflatoxins in Turkish dried figs for export to the European Union. Journal of Food Protection 70: 1029-1032.
'Aflatoxins in Turkish dried figs for export to the European Union ' () 70 Journal of Food Protection : 1029 -1032 .
-
Tam, J., Mankotia, M., Mably, M., Pantazopoulos, P. and Neil, R.J., 2006. Survey of breakfast and infant cereals for aflatoxins B1, B2, G1 and G2. Food Additives and Contaminants 23: 693-699.
'Survey of breakfast and infant cereals for aflatoxins B1, B2, G1 and G2 ' () 23 Food Additives and Contaminants : 693 -699 .
-
Yong-Fong, C., Ziling, L. and Uwe, N., 2001. Ultrafast liquid chromatography/ultraviolet and liquid chromatography/tandem mass spectrometric analysis. Rapid Communications in Mass Spectrometry 15: 141-151.
'Ultrafast liquid chromatography/ultraviolet and liquid chromatography/tandem mass spectrometric analysis ' () 15 Rapid Communications in Mass Spectrometry : 141 -151 .
| å ¨é¨æé´ | è¿å»ä¸å¹´ | è¿å»30天 | |
|---|---|---|---|
| æè¦æµè§æ¬¡æ° | 169 | 63 | 10 |
| å ¨ææµè§æ¬¡æ° | 23 | 2 | 1 |
| PDFä¸è½½æ¬¡æ° | 13 | 2 | 0 |
Rapid LC and LC/MS for routine analysis of mycotoxins in foods
äºWorld Mycotoxin JournalSearch for other papers by H. Åenyuva in
Current site
Google Scholar
PubMed
Search for other papers by J. Gilbert in
Current site
Google Scholar
PubMed
Search for other papers by S. Ãzcan in
Current site
Google Scholar
PubMed
Search for other papers by N. Gürel in
Current site
Google Scholar
PubMed
Affinity column clean-up of food samples for mycotoxin analysis produces extracts which are free of co-extractives and therefore require little chromatography for separation and quantification of the target analytes. Using such clean extracts, we report rapid chromatographic methods for aflatoxins B1, B2, G1 and G2, aflatoxin M1, ochratoxin A, zearalenone and fumonisins. Using short columns with small particle size packing, HPLC conditions have been developed reducing analysis time typically by 75%, e.g. for aflatoxins from 19.0 min to 4.0 min with full separation of the four toxins and for aflatoxin M1 giving an analysis time of less than 1 min compared to 5 min for conventional analysis. Fumonisins were analysed directly by LC/MS in a run time of 4.1 min, using selected ion monitoring to avoid the need for derivatisation for fluorescence detection by HPLC. Peak purity under rapid analysis conditions has been demonstrated by LC/MS for a variety of food extracts such as pistachios, hazelnuts, paprika, cocoa, coffee, cereals, sesame oil, cheese, maize, animal feed, dried figs, dried vine fruits, cornflakes and bread. These substantial reductions in analysis time offer significant benefits to laboratories undertaking routine screening and quantitative analysis of mycotoxins in foods.
| å ¨é¨æé´ | è¿å»ä¸å¹´ | è¿å»30天 | |
|---|---|---|---|
| æè¦æµè§æ¬¡æ° | 169 | 63 | 10 |
| å ¨ææµè§æ¬¡æ° | 23 | 2 | 1 |
| PDFä¸è½½æ¬¡æ° | 13 | 2 | 0 |
