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Selection and development of superior strains of Tenebrio molitor Linnaeus (Coleoptera: Tenebrionidae) with genetic diversity in South Korea

in Journal of Insects as Food and Feed
Autor:innen:
C.-O. Kim Department of Agricultural Biology, National Institute of Agricultural Sciences, Wanju 55365, South Korea

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https://orcid.org/0009-0005-6695-3976
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J.S. Park Department of Agricultural Biology, National Institute of Agricultural Sciences, Wanju 55365, South Korea

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https://orcid.org/0000-0003-4822-0996
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M. Shin Research Institute of Climate Change and Agriculture, National Institute of Horticultural and Herbal Science, Jeju 63240, South Korea

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S. Ji Department of Agricultural Biology, National Institute of Agricultural Sciences, Wanju 55365, South Korea

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https://orcid.org/0000-0001-8978-0880
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B. Koo Department of Agricultural Biology, National Institute of Agricultural Sciences, Wanju 55365, South Korea

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https://orcid.org/0000-0002-3283-8540
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S.Y. Kim Department of Agricultural Biology, National Institute of Agricultural Sciences, Wanju 55365, South Korea

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https://orcid.org/0000-0002-9953-0110
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K. Park Department of Agricultural Biology, National Institute of Agricultural Sciences, Wanju 55365, South Korea

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https://orcid.org/0000-0002-3147-8651
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Y.-S. Seok Gangwon-do Agricultural Product Registered Seed Station, Chuncheon 24410, South Korea

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J.-H. Song Department of Agricultural Biology, National Institute of Agricultural Sciences, Wanju 55365, South Korea

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https://orcid.org/0000-0003-0317-1457
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Abstract

Yellow mealworms (Tenebrio molitor Linnaeus) are very promising insects in the food and feed industry as an alternative protein source. However, their expanded application requires the development of improved strains for large-scale, stable production. We compared developmental, fecundity, and genetic characteristics of six strains (G101–G106), selected through preliminary analyses of 17 populations in South Korea. Each characteristic was evaluated over two consecutive generations (9 and 10). In the analysis of developmental characteristics, the G103 strain showed significantly higher larval and pupal weights and a shorter larval duration than those of the other strains. Furthermore, we assessed genetic diversity based on mitochondrial COI barcode sequences, including sequences retrieved from BOLD Systems and GenBank. The barcoding analysis revealed substantial genetic variation within T. molitor, including high genetic diversity within all strains and regions. Levels of nucleotide and haplogroup diversity within each strain were similar among all strains, indicating that G103 also maintains sufficient genetic diversity comparable to that of the other strains. These findings suggest that G103 is a candidate strain for the development of yellow mealworm.

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