Consequences of dietary amino acid imbalance for growth and gas exchange in black soldier fly (Hermetia illucens) larvae
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Abstract
Black soldier fly larvae (Hermetia illucens (L.); BSFL) production relies on efficient nutrient conversion into protein-rich biomass to ensure sustainability and economic viability. While the effects of dietary protein content and macronutrient ratios are well described, the metabolic costs of providing an imbalanced essential amino acid (EAA) supply in dietary proteins remain poorly understood. We examined how dietary EAA imbalance affects growth, metabolism, and energy allocation in BSFL. Larvae were reared on a reference diet (chicken feed) and three isonitrogenous, semi-synthetic diets: one balanced diet matching the EAA profile of the reference, and two unbalanced diets selectively deficient in either leucine, phenylalanine, and threonine or isoleucine, lysine, and tryptophan. Using open flow respirometry, daily growth monitoring, and biochemical analysis, we quantified gas exchange, body composition, and the energetic costs of biomass and protein deposition. EAA imbalance delayed development, reduced growth and protein content, and promoted lipid accumulation, while O2 consumption and CO2 production were reduced and respiratory exchange ratios exceeded 1.0, indicating elevated lipogenesis. Metabolic costs per unit protein deposited were higher on EAA-deficient diets (up to 3.63 kJ per kJ protein), reflecting inefficient amino acid utilisation. In contrast, the balanced diet supported more efficient protein deposition at a lower energetic cost (2.73 kJ per kJ protein). Our findings demonstrate that suboptimal dietary EAA profiles reduce metabolic efficiency in BSFL. Quantifying the physiological costs of amino acid imbalance provides benchmarks to guide feed formulations in insect production systems to minimise input costs and maximise the nutritional quality and quantity of larval output.
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-
Banks, I.J., Gibson, W.T. and Cameron, M.M., 2014. Growth rates of black soldier fly larvae fed on fresh human faeces and their implication for improving sanitation. Tropical Medicine and International Health 19: 14-22. https://doi.org/10.1111/tmi.12228
-
Barragan-Fonseca, K.B., Gort, G., Dicke, M. and van Loon, J.J.A., 2019. Effects of dietary protein and carbohydrate on life-history traits and body protein and fat contents of the black soldier fly Hermetia illucens. Physiological Entomology 44: 148-159. https://doi.org/10.1111/phen.12285
-
Berggreen, I.E., Schøn, M.L., Nørgaard, J.V. and Jensen, K., 2025. Optimal dietary protein content and essential amino acid limitation in larvae of the black soldier fly (Hermetia illucens). Journal of Insects as Food and Feed 11: 2579-2590. https://doi.org/10.1163/23524588-bja10221
-
Berner, D., Blanckenhorn, W.U. and Körner, C., 2005. Grasshoppers cope with low host plant quality by compensatory feeding and food selection: N limitation challenged. Oikos 111: 525-533. https://doi.org/10.1111/j.1600-0706.2005.14144.x
-
Broeckx, L., Frooninckx, L., Slegers, L., Berrens, S., Noyens, I., Goossens, S., Verheyen, G., Wuyts, A. and Van Miert, S., 2021. Growth of black soldier fly larvae reared on organic side-streams. Sustainability 13: 12953. https://doi.org/10.3390/su132312953
-
Cammack, J.A. and Tomberlin, J.K., 2017. The impact of diet protein and carbohydrate on select life-history traits of the black soldier fly Hermetia illucens (L.) (Diptera: Stratiomyidae). Insects 8: 56. https://doi.org/10.3390/insects8020056
-
Capuano, E., Oliviero, T., Fogliano, V. and Pellegrini, N., 2018. Role of the food matrix and digestion on calculation of the actual energy content of food. Nutrition Reviews 76: 274-289. https://doi.org/10.1093/nutrit/nux072
-
Davis, G.R.F., 1975. Essential dietary amino acids for growth of larvae of the yellow mealworm, Tenebrio molitor L. Journal of Nutrition 105: 1071-1075. https://doi.org/10.1093/jn/105.8.1071
-
Eggink, K.M., Donoso, I.G. and Dalsgaard, J., 2023. Optimal dietary protein to carbohydrate ratio for black soldier fly (Hermetia illucens) larvae. Journal of Insects as Food and Feed 9: 789-798. https://doi.org/10.3920/JIFF2022.0102
-
Eriksen, N.T., 2022. Dynamic modelling of feed assimilation, growth, lipid accumulation, and CO2 production in black soldier fly larvae. PLoS ONE 17: e0276605. https://doi.org/10.1371/journal.pone.0276605
-
Fuhrmann, A., Gold, M., Loh, R.K., Chu, C.X., Haberkorn, I., Puniamoorthy, N. and Mathys, A., 2025. Physical properties of food waste influence the efficiency of black soldier fly larvae bioconversion via microbial activity. Journal of Environmental Management 387: 125777. https://doi.org/10.1016/j.jenvman.2025.125777
-
Gligorescu, A., Toft, S., Hauggaard-Nielsen, H., Axelsen, J.A. and Nielsen, S.A., 2019. Development, growth and metabolic rate of Hermetia illucens larvae. Journal of Applied Entomology 143: 875-881. https://doi.org/10.1111/jen.12653
-
Gold, M., Tomberlin, J.K., Diener, S., Zurbrügg, C. and Mathys, A., 2018. Decomposition of biowaste macronutrients, microbes, and chemicals in black soldier fly larval treatment: a review. Waste Management 82: 302-318. https://doi.org/10.1016/j.wasman.2018.10.022
-
Goodrich, H.R., Wood, C.M., Wilson, R.W., Clark, T.D., Last, K.B. and Wang, T., 2024. Specific dynamic action: the energy cost of digestion or growth? Journal of Experimental Biology 227: jeb246722. https://doi.org/10.1242/jeb.246722
-
Hawkins, A.J.S., 1991. Protein turnover: a functional appraisal. Functional Ecology 5: 222-233. https://doi.org/10.2307/2389260
-
Jakobsen, K. and Thorbekt, G., 1993. The respiratory quotient in relation to fat deposition in fattening-growing pigs. British Journal of Nutrition 69: 333-343. https://doi.org/10.1079/bjn19930037
-
Janssen, R.H., Vincken, J.P., Van Den Broek, L.A.M., Fogliano, V. and Lakemond, C.M.M., 2017. Nitrogen-to-protein conversion factors for three edible insects: Tenebrio molitor, Alphitobius diaperinus, and Hermetia illucens. Journal of Agricultural and Food Chemistry 65: 2275-2278. https://doi.org/10.1021/acs.jafc.7b00471
-
Koethe, M., Taubert, J. and Vervuert, I., 2022. Impact of lysine supplementation on growth and development of Hermetia illucens larvae. Journal of Insects as Food and Feed 8: 35-44. https://doi.org/10.3920/JIFF2020.0059
-
Laganaro, M., Bahrndorff, S. and Eriksen, N.T., 2021. Growth and metabolic performance of black soldier fly larvae grown on low and high-quality substrates. Waste Management 121: 198-205. https://doi.org/10.1016/j.wasman.2020.12.009
-
Lee, K.P., 2007. The interactive effects of protein quality and macronutrient imbalance on nutrient balancing in an insect herbivore. Journal of Experimental Biology 210: 3236-3244. https://doi.org/10.1242/jeb.008060
-
Lemme, A. and Klüber, P., 2024. Rethinking amino acid nutrition of black soldier fly larvae (Hermetia illucens) based on insights from an amino acid reduction trial. Insects 15: 862. https://doi.org/10.3390/insects15110862
-
Liu, C., Wang, C. and Yao, H., 2019. Comprehensive resource utilization of waste using the black soldier fly (Hermetia illucens (L.)) (diptera: Stratiomyidae). Animals 9: 349. https://doi.org/10.3390/ani9060349
-
Lomonaco, G., Franco, A., De Smet, J., Scieuzo, C., Salvia, R. and Falabella, P., 2024. Larval frass of Hermetia illucens as organic fertilizer: composition and beneficial effects on different crops. Insects 15: 293. https://doi.org/10.3390/insects15040293
-
Oonincx, D.G.A.B., Gold, M., Bosch, G., Guillaume, J.B., Rumbos, C.I., El Deen, S.N., Sandrock, C., Bellezza Oddon, S., Athanassiou, C.G., Cambra-López, M., Pascual, J.J., Parodi, A.P., Spranghers, T. and Yakti, W., 2025. Bugbook: nutritional requirements for edible insect rearing. Journal of Insects as Food and Feed 11: S269-S288. https://doi.org/10.1163/23524588-bja10226
-
Oonincx, D.G.A.B., van Broekhoven, S., van Huis, A. and van Loon, J.J.A., 2015. Feed conversion, survival and development, and composition of four insect species on diets composed of food by-products. PLoS ONE 10: e0144601. https://doi.org/10.1371/journal.pone.0144601
-
Parodi, A., De Boer, I.J.M., Gerrits, W.J.J., Van Loon, J.J.A., Heetkamp, M.J.W., Van Schelt, J., Bolhuis, J.E. and Van Zanten, H.H.E., 2020. Bioconversion efficiencies, greenhouse gas and ammonia emissions during black soldier fly rearing – a mass balance approach. Journal of Cleaner Production 271: 122488. https://doi.org/10.1016/J.JCLEPRO.2020.122488
-
Pimentel, A.C., Montali, A., Bruno, D. and Tettamanti, G., 2017. Metabolic adjustment of the larval fat body in Hermetia illucens to dietary conditions. Journal of Asia-Pacific Entomology 20: 1307-1313. https://doi.org/10.1016/j.aspen.2017.09.017
-
Posit Team, 2023. RStudio: Integrated development environment for R (2023.9.1.494). Posit Software (PBC), Boston, MA. Available online at http://www.posit.co/
-
Purkayastha, D. and Sarkar, S., 2022. Sustainable waste management using black soldier fly larva: a review. International Journal of Environmental Science and Technology 19: 12701-12726. https://doi.org/10.1007/s13762-021-03524-7
-
R Core Team, 2023. A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna. Available online at https://www.R-project.org.
-
Raubenheimer, D. and Simpson, S.J., 1997. Integrative models of nutrient balancing: application to insects and vertebrates. Nutrition Research Reviews 10: 151-179. https://doi.org/10.1079/nrr19970009
-
Schmidt-Nilsen, K., 1997. Animal physiology: adaption and environment. Cambridge University Press, Cambridge.
-
Schneider, L., Kisinga, B., Stoehr, N., Cord-Landwehr, S., Schulte-Geldermann, E., Moerschbacher, B.M., Eder, K., Jha, R. and Dusel, G., 2025. Dietary protein levels in isoenergetic diets affect the performance, nutrient utilization and retention of nitrogen and amino acids of Hermetia illucens (L.) (Diptera: Stratiomyidae) larvae. Insects 16: 240. https://doi.org/10.3390/insects16030240
-
Schøn, M.L., Jensen, K., Schow-Madsen, M., Gold, M., Mathys, A., Berggreen, I.E., Schou, T.M., Nørgaard, J.V. and Overgaard, J., 2025a. Using gas exchange measurements to monitor growth, energy expenditure, and body composition of black soldier fly larvae (Hermetia illucens) on diets varying in protein-to-carbohydrate ratio. bioRxiv. https://doi.org/10.1101/2025.09.08.674872
-
Schøn, M.L., Mikkelsen, M.V.N., Jensen, K., Poulsen, J.M., Berggreen, I.E., Schou, T.M., Nørgaard, J.V. and Overgaard, J., 2025b. Effect of temperature on growth, metabolism, and gas exchange in Hermetia illucens larvae reared under commercial and laboratory conditions. Journal of Insects as Food and Feed 11: 1059-1074. https://doi.org/10.1163/23524588-00001268
-
Secor, S.M., 2009. Specific dynamic action: a review of the postprandial metabolic response. Journal of Comparative Physiology B 179: 1-56. https://doi.org/10.1007/s00360-008-0283-7
-
Siddiqui, S.A., Süfer, Ö., Çalışkan Koç, G., Lutuf, H., Rahayu, T., Castro-Muñoz, R. and Fernando, I., 2024. Enhancing the bioconversion rate and end products of black soldier fly (BSF) treatment – a comprehensive review. Environment, Development and Sustainability 27: 9673-9741. https://doi.org/10.1007/s10668-023-04306-6
-
Spranghers, T., Moradei, A., Vynckier, K., Boudrez, M., Pinotti, L. and Ottoboni, M., 2025. Amino acid requirements of yellow mealworm and black soldier fly larvae. Journal of Insects as Food and Feed 11: 1047-1058. https://doi.org/10.1163/23524588-00001271
-
Stryer, L., 1995. Biochemistry. W.H. Freeman, New York, NY.
-
Talal, S., Cease, A., Farington, R., Medina, H.E., Rojas, J. and Harrison, J., 2021. High carbohydrate diet ingestion increases post-meal lipid synthesis and drives respiratory exchange ratios above 1. Journal of Experimental Biology 224: jeb240010. https://doi.org/10.1242/jeb.240010
-
Talal, S., Harrison, J.F., Farington, R., Youngblood, J.P., Medina, H.E., Overson, R. and Cease, A.J., 2024. Body mass and growth rates predict protein intake across animals. eLife 12: e88933. https://doi.org/10.7554/eLife.88933
-
Tomberlin, J.K., Miranda, C., Flint, C., Harris, E. and Wu, G., 2023. Nutrients limit production of insects for food and feed: an emphasis on nutritionally essential amino acids. Animal Frontiers 13: 64-70. https://doi.org/10.1093/af/vfad032
-
van der Heide, M.E., Stødkilde, L., Nørgaard, J.V. and Studnitz, M., 2021. The potential of locally-sourced European protein sources for organic monogastric production: a review of forage crop extracts, seaweed, starfish, mussel, and insects. Sustainability 13: 2303. https://doi.org/10.3390/su13042303
-
Van’t Hof, H.M. and Martin, M.M., 1989. The effect of diet water content on energy expenditure by third-instar Manduca sexta larvae (Lepidoptera: Sphingidae). Journal of Insect Physiology 35: 433-436. https://doi.org/10.1016/0022-1910(89)90118-2
-
Von Bertalanffy, L., 1957. Quantitative laws in metabolism and growth. Quarterly Review of Biology 32: 217-231. https://doi.org/10.1086/401873
-
Withers, P.C., 2001. Design, calibration and calculation for flow-through respirometry systems. Australian Journal of Zoology 49: 445-461. https://doi.org/10.1071/ZO00057
-
Wu, G., Bazer, F.W., Dai, Z., Li, D., Wang, J. and Wu, Z., 2014. Amino acid nutrition in animals: protein synthesis and beyond. Annual Review of Animal Biosciences 2: 387-417. https://doi.org/10.1146/annurev-animal-022513-114113
Supplementary Materials
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Consequences of dietary amino acid imbalance for growth and gas exchange in black soldier fly (Hermetia illucens) larvae
In: Journal of Insects as Food and FeedSearch for other papers by M. Schow-Madsen in
Current site
Google Scholar
PubMed
Search for other papers by K. Jensen in
Current site
Google Scholar
PubMed
Search for other papers by M. Márquez-Toribio in
Current site
Google Scholar
PubMed
Department for Health Sciences and Technology, Sustainable Food Processing Laboratory, ETH Zurich, Schmelzbergstrasse 9, 8092 Zürich, Switzerland
Search for other papers by M.L. Schøn in
Current site
Google Scholar
PubMed
Search for other papers by T.M. Schou in
Current site
Google Scholar
PubMed
Search for other papers by J.V. Nørgaard in
Current site
Google Scholar
PubMed
Search for other papers by J. Overgaard in
Current site
Google Scholar
PubMed
Abstract
Black soldier fly larvae (Hermetia illucens (L.); BSFL) production relies on efficient nutrient conversion into protein-rich biomass to ensure sustainability and economic viability. While the effects of dietary protein content and macronutrient ratios are well described, the metabolic costs of providing an imbalanced essential amino acid (EAA) supply in dietary proteins remain poorly understood. We examined how dietary EAA imbalance affects growth, metabolism, and energy allocation in BSFL. Larvae were reared on a reference diet (chicken feed) and three isonitrogenous, semi-synthetic diets: one balanced diet matching the EAA profile of the reference, and two unbalanced diets selectively deficient in either leucine, phenylalanine, and threonine or isoleucine, lysine, and tryptophan. Using open flow respirometry, daily growth monitoring, and biochemical analysis, we quantified gas exchange, body composition, and the energetic costs of biomass and protein deposition. EAA imbalance delayed development, reduced growth and protein content, and promoted lipid accumulation, while O2 consumption and CO2 production were reduced and respiratory exchange ratios exceeded 1.0, indicating elevated lipogenesis. Metabolic costs per unit protein deposited were higher on EAA-deficient diets (up to 3.63 kJ per kJ protein), reflecting inefficient amino acid utilisation. In contrast, the balanced diet supported more efficient protein deposition at a lower energetic cost (2.73 kJ per kJ protein). Our findings demonstrate that suboptimal dietary EAA profiles reduce metabolic efficiency in BSFL. Quantifying the physiological costs of amino acid imbalance provides benchmarks to guide feed formulations in insect production systems to minimise input costs and maximise the nutritional quality and quantity of larval output.
| All Time | Past 365 days | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 218 | 218 | 30 |
| Full Text Views | 33 | 33 | 1 |
| PDF Views & Downloads | 74 | 74 | 2 |
