Viability ofDicrocoelium dendriticum metacercariae inFormica polyctena ants after exposure to different treatments
äºJournal of Insects as Food and FeedSearch for other papers by A.B. Jensen in
Current site
Google Scholar
Search for other papers by J. Malagocka in
Current site
Google Scholar
Search for other papers by J. Eilenberg in
Current site
Google Scholar
Search for other papers by B.L. Fredensborg in
Current site
Google Scholar
In the western world edible insects have gained increased recognition for their taste, nutritional value, and potential for commercial-scale production of foods for human consumption and animal feed. The presence of food-borne parasites in insects is therefore a concern due to the potential hazards it may cause consumers. In this study we developed a viability assay including trypsin treatment of trematodeDicrocoelium dendriticum metacercariae from the abdomen of the red wood antFormica polyctena. Then we tested the viability ofD. dendriticum metacecariae in relation to: (1) freezing (-20 and -80 °C); (2) boiling (100 °C); and (3) ethanol (50%). The metacercariae in all controlants were alive and displayed movements either in the cyst, or after excystation, whereas all metacercariae from all treatment groups were recorded as dead. Freezing at -20 or -80 °C for 30 min, boiling for 1 min or storage in 50% ethanol for 24 h proved effective in killing the lancet liver flukeD. dendriticum metacercariae.
Purchase
Buy instant access (PDF download and unlimited online access):
Institutional Login
Log in with Open Athens, Shibboleth, or your institutional credentials
Personal login
Log in with your brill.com account
-
Attygalle, A.B. and Morgan E.D., 1984. Chemicals from the glands of ants. Chemical Society Reviews 13: 245-278.
'Chemicals from the glands of ants ' () 13 Chemical Society Reviews : 245 -278.
-
Beldsoe, G.E. and Oria, M.P., 2001. Potential hazards in cold-smoked fish: parasites. Journal of Food Science 66: S1100-S1103.
'Potential hazards in cold-smoked fish: parasites ' () 66 Journal of Food Science : S1100 -S1103.
-
Borges, J.N., Skov, J., Bahlool, Q.Z.M., Møller, O.S., Kania, P.W., Santos, C.P. and Buchmann, K., 2015. Viability ofCryptocotyle lingua metacercariae from Atlantic cod (Gadus morhua) after exposure to freezing and heating in the temperature range from -80 °C to 100 °C. Food Control 50: 371-377.
'Viability ofCryptocotyle lingua metacercariae from Atlantic cod (Gadus morhua) after exposure to freezing and heating in the temperature range from -80 °C to 100 °C ' () 50 Food Control : 371 -377.
-
Botnevik, C.F., Malagocka, J., Jensen, A.B. and Fredensborg, B.L., 2016. Relative effects of temperature, light, and humidity on clinging behavior of metacercariae-infected ants. Journal of Parasitology 102: 495-500.
'Relative effects of temperature, light, and humidity on clinging behavior of metacercariae-infected ants ' () 102 Journal of Parasitology : 495 -500.
-
Bryant, C., 1998. Advances in trematode biology. Parasitology Today 14: 336.
'Advances in trematode biology ' () 14 Parasitology Today : 336.
-
Cengiz, Z.T., Yilmaz, H., Dulger, A.C. and Cicek, M., 2010. Human infection withDicrocoelium dendriticum in Turkey. Annals of Saudi Medicine 30: 159-161.
'Human infection withDicrocoelium dendriticum in Turkey ' () 30 Annals of Saudi Medicine : 159 -161.
-
Chung, Y.B., Kong, Y., Joo, I.J., Choo, S.Y. and Kang, S.Y., 1995. Excystment ofParagonimus westermani metacercariae by endogenous cysteine protease. Journal of Parasitology 81: 137-142.
'Excystment ofParagonimus westermani metacercariae by endogenous cysteine protease ' () 81 Journal of Parasitology : 137 -142.
-
el-Shiekh Mohamed, A.R. and Mummery, V., 1990. Human dicroceliasis: report on 208 cases from Saudi Arabia. Tropical and Geographical Medicine 42: 1-7.
'Human dicroceliasis: report on 208 cases from Saudi Arabia ' () 42 Tropical and Geographical Medicine : 1 -7.
-
Erasmus, D.A and Bennett, L.J., 1965. A study of some of the factors affecting excystationin vitro of the metacercarial stages ofHolostephanus lühei Szidat, 1936 andCyathocotyle bushiensis Khan, 1962 (Strigeida: Trematoda). Journal of Helminthology 39: 185-196.
'A study of some of the factors affecting excystationin vitro of the metacercarial stages ofHolostephanus lühei Szidat, 1936 andCyathocotyle bushiensis Khan, 1962 (Strigeida: Trematoda) ' () 39 Journal of Helminthology : 185 -196.
-
European Food Safety Authority (EFSA), 2004. Regulation (EC) No. 853/2004 of the European parliament and council of 29 of April 2004 laying down specific hygiene rules for food of animal origin. Official Journal of the European Communities L 226: 22.
-
European Food Safety Authority (EFSA), 2010. Panel on biological hazards (BIOHAZ) scientific opinion on risk assessment of parasites in fishery products. EFSA Journal 8: 1543.
-
European Food Safety Authority (EFSA) Scientific Committee, 2015. Scientific opinion on a risk profile related to production and consumption of insects as food and feed. EFSA Journal 13(4257): 1-60.
-
Evans, J., 2014. Labre larver og lækre insekter [Luscious larvae and delicious insects]. moMentum 4: 30-34.
'Labre larver og lækre insekter [Luscious larvae and delicious insects] ' () 4 moMentum : 30 -34.
-
Fan, P.C., 1998. Viability of metacercariae ofClonorchis sinensis in frozen or salted freshwater fish. International Journal for Parasitology 28: 603-605.
'Viability of metacercariae ofClonorchis sinensis in frozen or salted freshwater fish ' () 28 International Journal for Parasitology : 603 -605.
-
Gordon, C.A., McManus, D.P., Jones, M.K., Gray, D.J. and Gobert, G.N., 2016. The increase of exotic zoonotic helminth infections: the impact of urbanization, climate change and globalization. Advances in Parasitology 91: 311-397.
'The increase of exotic zoonotic helminth infections: the impact of urbanization, climate change and globalization ' () 91 Advances in Parasitology : 311 -397.
-
Hamed, M.G.E. and Elias, A.N., 1970. Effect of food-processing methods upon survival of the trematode Heterophyes sp. In flech of mullet caught from brackish Egyptian waters. Journal of Food Science 35: 386-388.
'Effect of food-processing methods upon survival of the trematode Heterophyes sp ' () 35 Journal of Food Science : 386 -388.
-
Helmy, M.M. and Al-Mathal, E.M., 2003. Human infection withDicrocoelium dendriticum in Riyadh district (Saudi Arabia). Journal of the Egyptian Society of Parasitology 33: 139-144.
'Human infection withDicrocoelium dendriticum in Riyadh district (Saudi Arabia) ' () 33 Journal of the Egyptian Society of Parasitology : 139 -144.
-
Hughes, D., 2012. Parasites and the superorganism. In: Hughes, D.P., Brodeur, J., Thomas, F. and Dawkins, R. (eds.) Host manipulation by parasites. Oxford University Press, Oxford, UK, pp. 140-154.
'Parasites and the superorganism ', in Host manipulation by parasites , () 140 -154.
-
Jeandron, A., Rinaldi, L., Abdyldaieva, G., Usubalieva, J., Steinmann, P., Cringoli, G. and Utzinger, J., 2011. Human infections withDicrocoelium dendriticum in Kyrgyzstan: the tip of the iceberg? Journal of Parasitology 97: 1170-1172.
'Human infections withDicrocoelium dendriticum in Kyrgyzstan: the tip of the iceberg? ' () 97 Journal of Parasitology : 1170 -1172.
-
Johnston, B.R. and Halton, D.W., 1981, Excystationin vitro ofBucephaloides gracilescens meatacercaria (Trematoda: Bucephalidae). Zeitschrift für Parasitenkunde 65: 71-78.
'Excystationin vitro ofBucephaloides gracilescens meatacercaria (Trematoda: Bucephalidae) ' () 65 Zeitschrift für Parasitenkunde : 71 -78.
-
Li, S., Chung, Y.B., Chung, B.S., Choi, M.H., Yu, J.R. and Hong, S.T., 2004. The involvement of the cysteine proteases ofClonorchis sinensis metacercariae in excystment. Parasitology Research 93: 36-40.
'The involvement of the cysteine proteases ofClonorchis sinensis metacercariae in excystment ' () 93 Parasitology Research : 36 -40.
-
Malagocka, J., 2016. Specialized pathogen of a social insect: dynamics of a unique host-pathogen interaction between fungusPandora formicae and the red wood antFormica polyctena. Ph.D. thesis, University of Copenhagen, København, Denmark, 117 pp.
-
Manga-González, M.Y., González-Lanza, C., Cabanas, E. and Campo, R., 2001. Contributions to and review of dicrocoeliosis, with special reference to the intermediate hosts ofDicrocoelium dendriticum. Parasitology 123: 91-114.
'Contributions to and review of dicrocoeliosis, with special reference to the intermediate hosts ofDicrocoelium dendriticum ' () 123 Parasitology : 91 -114.
-
Morgan, E.D., 2008. Chemical sorcery for sociality: exocrine secretions of ants (Hymenoptera: Formicidae). Myrmecological News 11: 79-90.
'Chemical sorcery for sociality: exocrine secretions of ants (Hymenoptera: Formicidae) ' () 11 Myrmecological News : 79 -90.
-
Ondriska, F., Sobota, K., Janosek, J. and Joklová, E., 1989. A rare case of human autochthonous dicroceliasis in Czechoslovakia. Bratislava Medical Journal 90: 467-469.
'A rare case of human autochthonous dicroceliasis in Czechoslovakia ' () 90 Bratislava Medical Journal : 467 -469.
-
Rack, J., Adusu, E. and Jelinek, T., 2004. Human infection withDicrocoelium dendriticum. Deutsche Medizinische Wochenschrift 129: 2538-2540.
'Human infection withDicrocoelium dendriticum ' () 129 Deutsche Medizinische Wochenschrift : 2538 -2540.
-
Roberts, L.S., Janovy, J. and Nadler, S., 2013. Foundations of parasitology (9th Ed.). McGraw-Hill, New York, NY, USA.
'Foundations of parasitology (9th Ed.)', ().
-
Romig, T., Lucius, R. and Frank, W., 1980. Cerebral larvae in the second intermediate host ofDicrocoelium dendriticum (Rudolphi, 1819) andDicrocoelium hospes Looss, 1907 (Trematodes, Dicrocoeliidae). Zeitrischrift fur Parasitenkunde 63: 277-286.
'Cerebral larvae in the second intermediate host ofDicrocoelium dendriticum (Rudolphi, 1819) andDicrocoelium hospes Looss, 1907 (Trematodes, Dicrocoeliidae) ' () 63 Zeitrischrift fur Parasitenkunde : 277 -286.
-
Searcey, N., Reinhard, K.J., Egarter-Vigl, E., Maixner, F., Piombino-Mascali, D., Zink, A.R., Van der Sanden, W., Gardner, S.L. and Bianucci, R., 2013. Parasitism of the Zweeloo woman: dicrocoeliasis evidenced in a Roman period bog mummy. International Journal of Paleopathology 3: 224-228.
'Parasitism of the Zweeloo woman: dicrocoeliasis evidenced in a Roman period bog mummy ' () 3 International Journal of Paleopathology : 224 -228.
-
Sing, A., Tybus, K. and Fackler, I., 2008. Acute urticaria associated withDicrocoelium dendriticum infestation. Indian Journal of Medical Microbiology 26: 97-98.
'Acute urticaria associated withDicrocoelium dendriticum infestation ' () 26 Indian Journal of Medical Microbiology : 97 -98.
-
Tragust, S., Mitteregger, B., Barone, V., Konrad, M., Ugelvig, L.V. and Cremer, S., 2013. Ants disinfect fungus-exposed brood by oral uptake and spread of their poison. Current Biology 23: 76-82.
'Ants disinfect fungus-exposed brood by oral uptake and spread of their poison ' () 23 Current Biology : 76 -82.
-
U.S. Food and Drug Administration (FDA), 2011. Fish and fishery products hazards and controls guidance (4th Ed.). University of Florida, Gainesville, FL, USA.
-
Van Huis, A., Dicke, M. and Van Loon, J.J.A., 2015. Insects to feed the world. Journal of insects as food feed 1: 3-5.
'Insects to feed the world ' () 1 Journal of insects as food feed : 3 -5.
-
Van Huis, A., Van Itterbeeck, J., Klunder, H., Mertens, E., Halloran, A., Muir, G. and Vantomme, P., 2013. Edible insects: future prospects for food and feed security. Food and Agriculture Organization of the United Nations (FAO), Rome, Italy, FAO Forestry Paper no. 171, 201 pp. Available at:http://www.fao.org/docrep/018/i3253e/i3253e.pdf.
| å ¨é¨æé´ | è¿å»ä¸å¹´ | è¿å»30天 | |
|---|---|---|---|
| æè¦æµè§æ¬¡æ° | 348 | 136 | 16 |
| å ¨ææµè§æ¬¡æ° | 16 | 1 | 0 |
| PDFä¸è½½æ¬¡æ° | 25 | 3 | 0 |
Viability ofDicrocoelium dendriticum metacercariae inFormica polyctena ants after exposure to different treatments
äºJournal of Insects as Food and FeedSearch for other papers by A.B. Jensen in
Current site
Google Scholar
PubMed
Search for other papers by J. Malagocka in
Current site
Google Scholar
PubMed
Search for other papers by J. Eilenberg in
Current site
Google Scholar
PubMed
Search for other papers by B.L. Fredensborg in
Current site
Google Scholar
PubMed
In the western world edible insects have gained increased recognition for their taste, nutritional value, and potential for commercial-scale production of foods for human consumption and animal feed. The presence of food-borne parasites in insects is therefore a concern due to the potential hazards it may cause consumers. In this study we developed a viability assay including trypsin treatment of trematodeDicrocoelium dendriticum metacercariae from the abdomen of the red wood antFormica polyctena. Then we tested the viability ofD. dendriticum metacecariae in relation to: (1) freezing (-20 and -80 °C); (2) boiling (100 °C); and (3) ethanol (50%). The metacercariae in all controlants were alive and displayed movements either in the cyst, or after excystation, whereas all metacercariae from all treatment groups were recorded as dead. Freezing at -20 or -80 °C for 30 min, boiling for 1 min or storage in 50% ethanol for 24 h proved effective in killing the lancet liver flukeD. dendriticum metacercariae.
| å ¨é¨æé´ | è¿å»ä¸å¹´ | è¿å»30天 | |
|---|---|---|---|
| æè¦æµè§æ¬¡æ° | 348 | 136 | 16 |
| å ¨ææµè§æ¬¡æ° | 16 | 1 | 0 |
| PDFä¸è½½æ¬¡æ° | 25 | 3 | 0 |
