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Protected black soldier fly larvae oil by calcium soap enhances rumen fermentation, gas production kinetics, and digestibility in vitro study

In: Journal of Insects as Food and Feed
Authors:
Hajrian Rizqi Albarki Tropical Feed Resources Research and Development Center (TROFREC), Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen 40002, Thailand

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Chanon Suntara Tropical Feed Resources Research and Development Center (TROFREC), Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen 40002, Thailand

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Sawitree Wongtangtintharn Tropical Feed Resources Research and Development Center (TROFREC), Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen 40002, Thailand

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Chuleemas Boonthai Iwai Department of Soil Science and Environment, Faculty of Agriculture, Khon Kaen University, Khon Kaen 40002, Thailand
Integrated Land and Water Resource Management Research and Development Center in Northeast Thailand, Khon Kaen University, Khon Kaen 40002, Thailand

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Anuraga Jayanegara Department of Nutrition and Feed Technology, Faculty of Animal Science, IPB University, 9 Bogor 16680, Indonesia
Animal Feed and Nutrition Modelling (AFENUE) Research Group, Department of Nutrition and Feed Technology, Faculty of Animal Science, IPB University, Bogor 16680, Indonesia

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Anusorn Cherdthong Tropical Feed Resources Research and Development Center (TROFREC), Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen 40002, Thailand

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https://orcid.org/0000-0002-8719-4404
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Abstract

Black soldier fly larvae oil (BSFLO) is a potential energy source but has problems with rumen toxicity. Therefore, the oil must be protected to maintain its fat quality and prevent negative effects on the microbial population. Therefore, this study investigated the effect of BSFLO protected by calcium (Ca) soap on rumen fermentation, gas production kinetics, and digestibility through an in vitro study. This in vitro study used rumen fluid obtained from five native Thai bulls (4.0–4.5 years old) weighing 450 ± 10 kg that had been adapted to a 14-days feeding period. This study used a completely randomized design with a 1 + 2 × 4 factorial arrangement, with the first factor being the treatment of BSFLO and Ca-soap BSFLO, the second factor being the BSFLO levels at 2, 4, 6, and 8% of dry matter (DM), and the plus one being the negative control. Through testing the effect of Ca-soap BSFLO and BSFLO with in vitro studies, it was found that Ca-soap BSFLO had better gas production compared to BSFLO in 24 and 48 h incubation, thus it was in line that Ca-soap can increase ( P < 0.05) digestibility both in the rumen and in the abomasum. In the product fermentation results parameters in 24 and 48 h incubation, Ca-soap BSFLO also increased ( P < 0.05) total ammonia-nitrogen, volatile fatty acid, and acetic acid and maintained the rumen pH. However, in 24 and 48 h incubation, Ca-soap BSFLO had no difference in rumen methane production and protozoan numbers compared to BSFLO. In conclusion, the results of this in vitro study demonstrate that Ca-soap BSFLO improved rumen fermentation and nutrient digestibility without significantly affecting methane emissions or protozoal populations.

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