Evaluation of the ultrafiltration method to obtain a high-value protein concentrate from the edible insect Tenebrio molitor
äºJournal of Insects as Food and FeedUniversidade CatoÌlica Portuguesa, CBQF â Centro de Biotecnologia e QuıÌmica Fina â LaboratoÌrio Associado, Escola Superior de Biotecnologia, Rua Diogo Botelho 1327, 4169-005, Porto, Portugal
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Abstract
The fractionation of edible insects is one of the major topics related to using insects as food. Developing adequate protein recovery methods is essential to guarantee acceptable incorporation of insect protein fractions into functional food products, which in turn is essential to the development of insect-based products with higher consumer acceptance. This research aimed to produce high-purity protein concentrates from Tenebrio molitor larvae with favourable techno-functional properties, through membrane ultrafiltration with a 50 kDa cut-off, while also comparing with protein concentrates obtained through isoelectric point precipitation (IP). The protein fractions were evaluated for extraction efficiency (protein content, yield, and recovery rate), protein profile (size exclusion chromatography), and techno-functional properties (colour, foaming, and emulsifying properties, and water/oil absorption capacities). The >50 kDa fraction had a protein content above 80% (although lower than the IP fraction), while the <50 kDa fraction only had a protein content of 44.2%. Despite its high protein content, the >50 kDa fraction only attained a protein recovery rate of 27.9%, comparable to the IP fraction recovery rate. The >50 kDa fraction had higher
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Alonso-Miravalles, L., Jeske, S., Bez, J., Detzel, A., Busch, M., Krueger, M., Wriessnegger, C.L., OâMahony, J.A., Zannini, E. and Arendt, E.K., 2019. Membrane filtration and isoelectric precipitation technological approaches for the preparation of novel, functional and sustainable protein isolate from lentils. European Food Research and Technology 245: 1855-1869. https://doi.org/10.1007/s00217-019-03296-y
-
Anusha, S. and Negi, P.S., 2023. Characterization and techno-functional properties of Tenebrio molitor larvae protein concentrate. Food Bioscience 54: 102882. https://doi.org/10.1016/j.fbio.2023.102882
-
Ardoin, R. and Prinyawiwatkul, W., 2020. Product appropriateness, willingness to try and perceived risks of foods containing insect protein powder: a survey of U.S. consumers. International Journal of Food Science & Technology 55: 3215-3226. https://doi.org/10.1111/ijfs.14612
-
Azagoh, C., Ducept, F., Garcia, R., Rakotozafy, L., Cuvelier, M.E., Keller, S., Lewandowski, R. and Mezdour, S., 2016. Extraction and physicochemical characterization of Tenebrio molitor proteins. Food Research International 88: 24-31. https://doi.org/10.1016/j.foodres.2016.06.010
-
Baiano, A., 2020. Edible insects: an overview on nutritional characteristics, safety, farming, production technologies, regulatory framework, and socio-economic and ethical implications. Trends in Food Science & Technology 100: 35-50. https://doi.org/10.1016/j.tifs.2020.03.040
-
Baldasso, C., Barros, T.C. and Tessaro, I.C., 2011. Concentration and purification of whey proteins by ultrafiltration. Desalination 278: 381-386. https://doi.org/10.1016/j.desal.2011.05.055
-
Barton, A., Richardson, C.D. and McSweeney, M.B., 2020. Consumer attitudes toward entomophagy before and after evaluating cricket (Acheta domesticus)-based protein powders. Journal of Food Science 85: 781-788. https://doi.org/10.1111/1750-3841.15043
-
BuÃler, S., Rumpold, B.A., Jander, E., Rawel, H.M. and Schlüter, O.K., 2016. Recovery and techno-functionality of flours and proteins from two edible insect species: meal worm (Tenebrio molitor) and black soldier fly (Hermetia illucens) larvae. Heliyon 2: e00218. https://doi.org/10.1016/j.heliyon.2016.e00218
-
Cho, H.-R. and Lee, S.-O., 2020. Novel hepatoprotective peptides derived from protein hydrolysates of mealworm (Tenebrio molitor). Food Research International 133: 109194. https://doi.org/10.1016/j.foodres.2020.109194
-
Clarkson, C., Mirosa, M. and Birch, J., 2018. Potential of extracted Locusta migratoria protein fractions as value-added ingredients. Insects 9: 20. https://doi.org/10.3390/insects9010020
-
Cunha, L.M. and Ribeiro, J.C., 2019. Sensory and consumer perspectives on edible insects. In: Sogari, G., Mora, C. and Menozzi, D. (eds.) Edible insects in the food sector: methods, current applications and perspectives. Springer International Publishing, Cham, Switzerlansd, pp. 57-71. https://doi.org/10.1007/978-3-030-22522-3_5
-
de Matos, F.M., Novelli, P.K. and de Castro, R.J.S., 2021. Enzymatic hydrolysis of black cricket (Gryllus assimilis) proteins positively affects their antioxidant properties. Journal of Food Science 86: 571-578. https://doi.org/10.1111/1750-3841.15576
-
EFSA Panel on Nutrition, N.F., Allergens, F., Turck, D., Bohn, T., Castenmiller, J., De Henauw, S., Hirsch-Ernst, K.I., Maciuk, A., Mangelsdorf, I., McArdle, H.J., Naska, A., Pelaez, C., Pentieva, K., Siani, A., Thies, F., Tsabouri, S., Vinceti, M., Cubadda, F., Frenzel, T., Heinonen, M., Marchelli, R., Neuhäuser-Berthold, M., Poulsen, M., Prieto Maradona, M., Schlatter, J.R., van Loveren, H., Ververis, E. and Knutsen, H.K., 2021. Safety of frozen and dried formulations from whole yellow mealworm (Tenebrio molitor larva) as a novel food pursuant to Regulation (EU) 2015/2283. EFSA Journal 19: e06778. https://doi.org/10.2903/j.efsa.2021.6778
-
El Nasri, N.A. and El Tinay, A.H., 2007. Functional properties of fenugreek (Trigonella foenum graecum) protein concentrate. Food Chemistry 103: 582-589. https://doi.org/10.1016/j.foodchem.2006.09.003
-
Gkinali, A.-A., Matsakidou, A. and Paraskevopoulou, A., 2022a. Characterization of Tenebrio molitor larvae protein preparations obtained by different extraction approaches. Foods 11: 3852. https://doi.org/10.3390/foods11233852
-
Gkinali, A.-A., Matsakidou, A., Vasileiou, E. and Paraskevopoulou, A., 2022b. Potentiality of Tenebrio molitor larva-based ingredients for the food industry: a review. Trends in Food Science & Technology 119: 495-507. https://doi.org/10.1016/j.tifs.2021.11.024
-
Gravel, A., Marciniak, A., Couture, M. and Doyen, A., 2021. Effects of hexane on protein profile, solubility and foaming properties of defatted proteins extracted from Tenebrio molitor larvae. Molecules 26: 351. https://doi.org/10.3390/molecules26020351
-
Hadidi, M., Khaksar, F.B., Pagan, J. and Ibarz, A., 2020. Application of Ultrasound-Ultrafiltration-Assisted alkaline isoelectric precipitation (UUAAIP) technique for producing alfalfa protein isolate for human consumption: optimization, comparison, physicochemical, and functional properties. Food Research International 130: 108907. https://doi.org/10.1016/j.foodres.2019.108907
-
Jantzen da Silva Lucas, A., Menegon de Oliveira, L., da Rocha, M. and Prentice, C., 2020. Edible insects: an alternative of nutritional, functional and bioactive compounds. Food Chemistry 311: 126022. https://doi.org/10.1016/j.foodchem.2019.126022
-
Jiang, Y.L., Zhu, Y.F., Zheng, Y.R., Liu, Z.M., Zhong, Y., Deng, Y. and Zhao, Y.Y., 2021. Effects of salting-in/out-assisted extractions on structural, physicochemical and functional properties of Tenebrio molitor larvae protein isolates. Food Chemistry 338: 128158. https://doi.org/10.1016/j.foodchem.2020.128158
-
Kim, T.K., Yong, H.I., Chun, H.H., Lee, M.A., Kim, Y.B. and Choi, Y.S., 2020. Changes of amino acid composition and protein technical functionality of edible insects by extracting steps. Journal of Asia-Pacific Entomology 23: 298-305. https://doi.org/10.1016/j.aspen.2019.12.017
-
Kröger, T., Dupont, J., Büsing, L. and Fiebelkorn, F., 2021. Acceptance of insect-based food products in Western societies: a systematic review. Frontiers in Nutrition 8: 759885. https://doi.org/10.3389/fnut.2021.759885
-
La Barbera, F., Verneau, F., Amato, M. and Grunert, K., 2018. Understanding Westernersâ disgust for the eating of insects: the role of food neophobia and implicit associations. Food Quality and Preference 64: 120-125. https://doi.org/10.1016/j.foodqual.2017.10.002
-
Laroche, M., Perreault, V., Marciniak, A., Gravel, A., Chamberland, J. and Doyen, A., 2019. Comparison of conventional and sustainable lipid extraction methods for the production of oil and protein isolate from edible insect meal. Foods 8: 572. https://doi.org/10.3390/foods8110572
-
Laroche, M., Perreault, V., Marciniak, A., Mikhaylin, S. and Doyen, A., 2022. Eco-efficiency of mealworm (Tenebrio molitor) protein extracts. ACS Food Science & Technology 2: 1077-1085. https://doi.org/10.1021/acsfoodscitech.2c00014
-
Melgar-Lalanne, G., HernaÌndez-AÌlvarez, A.-J. and Salinas-Castro, A., 2019. Edible insects processing: traditional and innovative technologies. Comprehensive Reviews in Food Science and Food Safety 18: 1166-1191. https://doi.org/10.1111/1541-4337.12463
-
Menozzi, D., Sogari, G., Veneziani, M., Simoni, E. and Mora, C., 2017. Eating novel foods: an application of the Theory of Planned Behaviour to predict the consumption of an insect-based product. Food Quality and Preference 59: 27-34. https://doi.org/10.1016/j.foodqual.2017.02.001
-
Mikhaylin, S., Patouillard, L., Margni, M. and Bazinet, L., 2018. Milk protein production by a more environmentally sustainable process: bipolar membrane electrodialysis coupled with ultrafiltration. Green Chemistry 20: 449-456. https://doi.org/10.1039/C7GC02154B
-
Mintah, B.K., He, R.H., Agyekum, A.A., Dabbour, M., Golly, M.K. and Ma, H.L., 2020. Edible insect protein for food applications: extraction, composition, and functional properties. Journal of Food Process Engineering 43: e13362. https://doi.org/10.1111/jfpe.13362
-
Mishyna, M., Keppler, J.K. and Chen, J., 2021. Techno-functional properties of edible insect proteins and effects of processing. Current Opinion in Colloid & Interface Science 56: 101508. https://doi.org/10.1016/j.cocis.2021.101508
-
Mishyna, M., Martinez, J.J.I., Chen, J.S. and Benjamin, O., 2019. Extraction, characterization and functional properties of soluble proteins from edible grasshopper (Schistocerca gregaria) and honey bee (Apis mellifera). Food Research International 116: 697-706. https://doi.org/10.1016/j.foodres.2018.08.098
-
Ndiritu, A.K., Kinyuru, J.N., Kenji, G.M. and Gichuhi, P.N., 2017. Extraction technique influences the physico-chemical characteristics and functional properties of edible crickets (Acheta domesticus) protein concentrate. Journal of Food Measurement and Characterization 11: 2013-2021. https://doi.org/10.1007/s11694-017-9584-4
-
Nikbakht Nasrabadi, M., Sedaghat Doost, A. and Mezzenga, R., 2021. Modification approaches of plant-based proteins to improve their techno-functionality and use in food products. Food Hydrocolloids 118: 106789. https://doi.org/10.1016/j.foodhyd.2021.106789
-
Nongonierma, A.B. and FitzGerald, R.J., 2017. Unlocking the biological potential of proteins from edible insects through enzymatic hydrolysis: a review. Innovative Food Science & Emerging Technologies 43: 239-252. https://doi.org/10.1016/j.ifset.2017.08.014
-
Orsi, L., Voege, L.L. and Stranieri, S., 2019. Eating edible insects as sustainable food? Exploring the determinants of consumer acceptance in Germany. Food Research International 125: 108573. https://doi.org/10.1016/j.foodres.2019.108573
-
Osimani, A., Garofalo, C., MilanovicÌ, V., Taccari, M., Cardinali, F., Aquilanti, L., Pasquini, M., Mozzon, M., Raffaelli, N., Ruschioni, S., Riolo, P., Isidoro, N. and Clementi, F., 2017. Insight into the proximate composition and microbial diversity of edible insects marketed in the European Union. European Food Research and Technology 243: 1157-1171. https://doi.org/10.1007/s00217-016-2828-4
-
Pasini, G., Cullere, M., Vegro, M., Simonato, B. and Dalle Zotte, A., 2022. Potentiality of protein fractions from the house cricket (Acheta domesticus) and yellow mealworm (Tenebrio molitor) for pasta formulation. LWT-Food Science and Technology 164: 113638. https://doi.org/10.1016/j.lwt.2022.113638
-
Purschke, B., Sanchez, Y.D.M. and Jager, H., 2018a. Centrifugal fractionation of mealworm larvae (Tenebrio molitor, L.) for protein recovery and concentration. LWT-Food Science and Technology 89: 224-228. https://doi.org/10.1016/j.lwt.2017.10.057
-
Purschke, B., Tanzmeister, H., Meinlschmidt, P., Baumgartner, S., Lauter, K. and Jager, H., 2018b. Recovery of soluble proteins from migratory locust (Locusta migratoria) and characterisation of their compositional and techno-functional properties. Food Research International 106: 271-279. https://doi.org/10.1016/j.foodres.2017.12.067
-
Queiroz, L.S., Nogueira Silva, N.F., Jessen, F., Mohammadifar, M.A., Stephani, R., Fernandes de Carvalho, A., Perrone, IÌ.T. and Casanova, F., 2023. Edible insect as an alternative protein source: a review on the chemistry and functionalities of proteins under different processing methods. Heliyon 9: e14831. https://doi.org/10.1016/j.heliyon.2023.e14831
-
Rahman, M.M., Byanju, B. and Lamsal, B.P., 2023. Protein, lipid, and chitin fractions from insects: method of extraction, functional properties, and potential applications. Critical Reviews in Food Science & Nutrition: 1-17. https://doi.org/10.1080/10408398.2023.2168620
-
Reverberi, M., 2021. The new packaged food products containing insects as an ingredient. Journal of Insects as Food and Feed 7: 901-908. https://doi.org/10.3920/jiff2020.0111
-
Ribeiro, J.C., Gonçalves, A.T.S., Moura, A.P., Varela, P. and Cunha, L.M., 2022a. Insects as food and feed in Portugal and Norway â cross-cultural comparison of determinants of acceptance. Food Quality and Preference 102: 104650. https://doi.org/10.1016/j.foodqual.2022.104650
-
Ribeiro, J.C., Lima, R.C., Maia, M.R.G., Almeida, A.A., Fonseca, A.J.M., Cabrita, A.R.J. and Cunha, L.M., 2019. Impact of defatting freeze-dried edible crickets (Acheta domesticus and Gryllodes sigillatus) on the nutritive value, overall liking and sensory profile of cereal bars. LWT-Food Science and Technology 113: 108335. https://doi.org/10.1016/j.lwt.2019.108335
-
Ribeiro, J.C., Santos, C., Lima, R.C., Pintado, M.E. and Cunha, L.M., 2022b. Impact of defatting and drying methods on the overall liking and sensory profile of a cereal bar incorporating edible insect species. Future Foods 6: 100190. https://doi.org/10.1016/j.fufo.2022.100190
-
Rossi, S., Parrotta, L., Gottardi, D., Glicerina, V.T., Duca, S.D., Rosa, M.D., Patrignani, F., Schlüter, O. and Lanciotti, R., 2022. Unravelling the potential of cricket-based hydrolysed sourdough on the quality of an innovative bakery product. Journal of Insects as Food and Feed 8: 921-935. https://doi.org/10.3920/JIFF2021.0184
-
Ruby, M.B., Rozin, P. and Chan, C., 2015. Determinants of willingness to eat insects in the USA and India. Journal of Insects as Food and Feed 1: 215-225. https://doi.org/10.3920/JIFF2015.0029
-
Santos, C., Ribeiro, J.C. and Cunha, L.M., 2021. Impacto de diferentes teÌcnicas de processamento na cor, mateÌria seca e atividade da aÌgua de larvas de Tenebrio molitor. CAPTAR. https://doi.org/10.34624/captar.v0i0.22945
-
Sere, A., Bougma, A., Bazie, B.S.R., Traore, E., Parkouda, C., Gnankine, O. and Bassole, I.H.N., 2021. Chemical composition, energy and nutritional values, digestibility and functional properties of defatted flour, protein concentrates and isolates from Carbula marginella (Hemiptera: Pentatomidae) and Cirina butyrospermi (Lepidoptera: Saturniidae). Bmc Chemistry 15: 46. https://doi.org/10.1186/s13065-021-00772-z
-
Tessari, P., Lante, A. and Mosca, G., 2016. Essential amino acids: master regulators of nutrition and environmental footprint? Scientific Reports 6: 26074. https://doi.org/10.1038/srep26074
-
Udomsil, N., Imsoonthornruksa, S., Gosalawit, C. and Ketudat-Cairns, M., 2019. Nutritional values and functional poperties of house cricket (Acheta domesticus) and field cricket (Gryllus bimaculatus). Food Science and Technology Research 25: 597-605. https://doi.org/10.3136/fstr.25.597
-
van Huis, A., 2022. Edible insects: challenges and prospects. Entomological Research 52: 161-177. https://doi.org/10.1111/1748-5967.12582
-
Wang, J.J., Jousse, M., Jayakumar, J., Fernandez-Arteaga, A., De Lamo-Castellvi, S., Ferrando, M. and Guell, C., 2021. Black soldier fly (Hermetia illucens) protein concentrates as a sustainable source to stabilize O/W emulsions produced by a low-energy high-throughput emulsification tTechnology. Foods 10: 1048. https://doi.org/10.3390/foods10051048
-
Wendin, K., Berg, J., Jönsson, K.I., Andersson, P., Birch, K., Davidsson, F., Gerberich, J., Rask, S. and Langton, M., 2021. Introducing mealworm as an ingredient in crisps and paÌteÌs â sensory characterization and consumer liking. Future Foods 4: 100082. https://doi.org/10.1016/j.fufo.2021.100082
-
Yi, L., Boekel, M.A.J.S.V. and Lakemond, C.M.M., 2017. Extracting Tenebrio molitor protein while preventing browning: effect of pH and NaCl on protein yield. Journal of Insects as Food and Feed 3: 21-31. https://doi.org/10.3920/jiff2016.0015
-
Yi, L., Lakemond, C.M., Sagis, L.M., Eisner-Schadler, V., van Huis, A. and van Boekel, M.A., 2013. Extraction and characterisation of protein fractions from five insect species. Food Chemistry 141: 3341-3348. https://doi.org/10.1016/j.foodchem.2013.05.115
-
Yoon, S., Wong, N.A.K., Chae, M. and Auh, J.H., 2019. Comparative characterization of protein hydrolysates from three edible insects: mealworm larvae, adult crickets, and silkworm pupae. Foods 8: 563. https://doi.org/10.3390/foods8110563
-
Yu, X., Oh, E. and Kim, Y., 2021. A comparative study on the functional properties of mealworm (Tenebrio molitor) larvae and soybean protein isolates and hydrolysates. International Food Research Journal 28: 816-826.
-
Zhao, X., Vazquez-Gutierrez, J.L., Johansson, D.P., Landberg, R. and Langton, M., 2016. Yellow mealworm protein for food purposes â extraction and functional properties. PlOS ONE 11: e0147791. https://doi.org/10.1371/journal.pone.0147791
-
Zielinska, E., Karas, M. and Baraniak, B., 2018. Comparison of functional properties of edible insects and protein preparations thereof. LWT-Food Science and Technology 91: 168-174. https://doi.org/10.1016/j.lwt.2018.01.058
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Evaluation of the ultrafiltration method to obtain a high-value protein concentrate from the edible insect Tenebrio molitor
äºJournal of Insects as Food and FeedUniversidade CatoÌlica Portuguesa, CBQF â Centro de Biotecnologia e QuıÌmica Fina â LaboratoÌrio Associado, Escola Superior de Biotecnologia, Rua Diogo Botelho 1327, 4169-005, Porto, Portugal
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Abstract
The fractionation of edible insects is one of the major topics related to using insects as food. Developing adequate protein recovery methods is essential to guarantee acceptable incorporation of insect protein fractions into functional food products, which in turn is essential to the development of insect-based products with higher consumer acceptance. This research aimed to produce high-purity protein concentrates from Tenebrio molitor larvae with favourable techno-functional properties, through membrane ultrafiltration with a 50 kDa cut-off, while also comparing with protein concentrates obtained through isoelectric point precipitation (IP). The protein fractions were evaluated for extraction efficiency (protein content, yield, and recovery rate), protein profile (size exclusion chromatography), and techno-functional properties (colour, foaming, and emulsifying properties, and water/oil absorption capacities). The >50 kDa fraction had a protein content above 80% (although lower than the IP fraction), while the <50 kDa fraction only had a protein content of 44.2%. Despite its high protein content, the >50 kDa fraction only attained a protein recovery rate of 27.9%, comparable to the IP fraction recovery rate. The >50 kDa fraction had higher
| å ¨é¨æé´ | è¿å»ä¸å¹´ | è¿å»30天 | |
|---|---|---|---|
| æè¦æµè§æ¬¡æ° | 1061 | 257 | 27 |
| å ¨ææµè§æ¬¡æ° | 41 | 9 | 0 |
| PDFä¸è½½æ¬¡æ° | 78 | 19 | 0 |
