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The effect of eight weeks of high-intensity interval training combined with L-citrulline malate supplementation on muscle damage, inflammation and physical fitness factors of volleyball girls

In: Comparative Exercise Physiology
Authors:
M. Norozi Department of Sport Sciences, Hakim Sabzevari University, Sabzevar, Iran

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K. Hejazi Department of Sport Sciences, Hakim Sabzevari University, Sabzevar, Iran

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H. Marefati Department of Sport Sciences, Hakim Sabzevari University, Sabzevar, Iran

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Abstract

High-intensity interval training (HIIT) is recognised as an effective method for improving physical fitness. L-citrulline malate supplementation has also gained attention among athletes due to its role in enhancing blood flow and reducing muscle fatigue. The aim of this study was to investigate the effects of an eight-week HIIT program combined with L-citrulline malate supplementation on muscle damage markers, inflammation, and physical fitness factors in female volleyball players. In this semi-experimental study, 20 female volleyball players aged 16-20 years were randomly divided into two groups: volleyball training with placebo (10 participants) and volleyball training with L-citrulline malate supplementation (10 participants). Both groups underwent an eight-week HIIT program, three sessions per week, with each session consisting of two sets of three 2-min intervals at 80-90% of maximum heart rate. The supplementation group consumed 2 g of L-citrulline malate powder dissolved in 200 ml of water daily. One-way repeated measures ANOVA was used to analyse the within-group and between-group changes. The findings showed no significant time × group interaction for creatine kinase, lactate dehydrogenase, alanine aminotransferase, and aspartate aminotransferase levels; however, C-reactive protein levels showed a significant interaction. Creatine kinase, lactate dehydrogenase, alanine aminotransferase, and aspartate aminotransferase levels significantly decreased in the group that received L-citrulline malate supplementation, while creatine kinase levels significantly increased in the placebo group. Volleyball training combined with L-citrulline malate supplementation improved muscle damage markers, inflammation, and physical fitness factors in female volleyball players, which is highly significant in volleyball. The results of this study highlight the potential beneficial role of L-citrulline malate supplementation in volleyball training programs.

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