Single bout of a long-duration running treadmill increases myoglobin but not haemoglobin and interleukin 6 levels in mice (Mus musculus)
äºComparative Exercise PhysiologyDepartment of Health and Recreation Education, Faculty of Sport Science, Universitas Negeri Surabaya, Lidah Wetan Street, East Java 60213, Indonesia
Search for other papers by D. Yuliastrid in
Current site
Google Scholar
Search for other papers by N.W. Kusnanik in
Current site
Google Scholar
Search for other papers by B. Purwanto in
Current site
Google Scholar
Search for other papers by A. Noordia in
Current site
Google Scholar
Search for other papers by S.P. Purwoto in
Current site
Google Scholar
Search for other papers by A. Pranoto in
Current site
Google Scholar
Abstract
Endurance was essential to perform an activity for as long as possible. Previously, haemoglobin, myoglobin, lactate and interleukin (IL)-6 level played a key role in determining endurance. This study aims to analyse the differences in the duration of running a treadmill on the levels of haemoglobin, myoglobin, lactate, and IL-6 in mice (Mus musculus). The mice were forced to run on the treadmill for as long as possible. They were divided into 3 groups, namely Short (n = 12; â¤12.5 min treadmill running), Moderate (n = 12; 12.5-40 min treadmill running), and Long (n = 12; â¥40 min treadmill running). Haemoglobin and lactate levels were evaluated, respectively, using the Quick-check Plus Hb Haemoglobin Testing System and Accutrend® Plus system. Analysis of myoglobin and IL-6 levels used an enzyme-linked immunosorbent assay (ELISA) kit. Statistical analysis used the one-way ANOVA, and LSD post-hoc tests with a significance level of 5%. The results showed that only the myoglobin level characterised endurance. Higher myoglobin levels were found at longer duration of running. Haemoglobin, lactate and IL-6 levels did not differ between groups. Based on the results of the study it was concluded that a longer duration of running was found a higher level of myoglobin. Myoglobin but not haemoglobin represented as an essential characteristic for endurance among runners in mice. High-affinity binding between myoglobin and oxygen was essential for oxygen storage in muscle.
Purchase
Buy instant access (PDF download and unlimited online access):
Institutional Login
Log in with Open Athens, Shibboleth, or your institutional credentials
Personal login
Log in with your brill.com account
-
Ahmed, M.H., Ghatge, M.S. and Safo, M.K., 2020. Hemoglobin: structure, function and allostery. In: Hoeger, U. and Harris, J.R. (eds.) Vertebrate and invertebrate respiratory proteins, lipoproteins and other body fluid proteins, subcellular biochemistry. Springer International Publishing, Cham, Switzerland, pp. 345-382. https://doi.org/10.1007/978-3-030-41769-7_14.
-
Andari, N.N.I., Dieny, F.F., Tsani, A.F.A., Fitranti, D.Y. and Widyastuti, N., 2021. Diet quality, nutritional status and haemoglobin level of female adolescent athletes in endurance and non endurance sports. Amerta Nutrition 5: 140-148. https://doi.org/10.20473/amnt.v5i2.2021.140-148.
-
Andarianto, A., Rejeki, P.S., Sakina, Pranoto, A., Seputra, T.W.A., Sugiharto and Miftahussurur, M., 2022. Inflammatory markers in response to interval and continuous exercise in obese women. Comparative Exercise Physiology 18: 135-142. https://doi.org/10.3920/CEP210038.
-
Antoni, M.F., Rejeki, P.S., Sulistiawati, Pranoto, A., Wigati, K.W., Sari, G.M., Lesmana, R. and Yamaoka, Y., 2022. Effect of nocturnal and diurnal moderate-intensity swimming exercise on increasing irisin level of female mice (Mus musculus). Chiang Mai University Journal of Natural Sciences 21: e2022033. https://doi.org/10.12982/CMUJNS.2022.033.
-
Baba, S., Takashima, T., Hirota, M., Kawashima, M. and Horikawa, E., 2017. Relationship between pulmonary function and elevated glycated hemoglobin levels in health checkups: a cross-sectional observational study in Japanese participants. Journal of Epidemiology 27: 511-515. https://doi.org/10.1016/j.je.2016.10.008.
-
Balducci, P., CleÌmençon, M., Morel, B., Quiniou, G., Saboul, D. and Hautier, C.A., 2016. Comparison of level and graded treadmill tests to evaluate endurance mountain runners. Journal of Sports Science and Medicine 15: 239-246.
-
Calbet, J.A.L., RaÌdegran, G., Boushel, R., Søndergaard, H., Saltin, B. and Wagner, P.D., 2002. Effect of blood haemoglobin concentration on V(O2,max) and cardiovascular function in lowlanders acclimatised to 5260 m. Journal of Physiology 545: 715-728. https://doi.org/10.1113/jphysiol.2002.029108.
-
Carson, B.P., 2017. The potential role of contraction-induced myokines in the regulation of metabolic function for the prevention and treatment of type 2 diabetes. Frontiers in Endocrinology 8: 97. https://doi.org/10.3389/fendo.2017.00097.
-
Cech, D.J. and Martin, S., 2012. Functional movement development across the life span. 3rd ed. Saunders Elsevier Inc, Philadelphia, PA, USA. https://doi.org/10.1016/C2009-0-60730-3.
-
Cheng, A.J., Place, N. and Westerblad, H., 2018. Molecular basis for exercise-induced fatigue: the importance of strictly controlled cellular Ca2+ handling. Cold Spring Harbor Perspectives in Medicine 8: a029710. https://doi.org/10.1101/cshperspect.a029710.
-
Conner, J.D., Wolden-Hanson, T. and Quinn, L.S., 2014. Assessment of murine exercise endurance without the use of a shock grid: an alternative to forced exercise. Journal of Visualized Experiments 90: e51846. https://doi.org/10.3791/51846.
-
Ferrari, M., Manea, L., Anton, K., Bruzzone, P., Meneghello, M., Zamboni, F., Purgato, L., Cazzoletti, L., Ferrari, P. and Testi, R., 2015. Anemia and hemoglobin serum levels are associated with exercise capacity and quality of life in chronic obstructive pulmonary disease. BMC Pulmonary Medicine 15: 58. https://doi.org/10.1186/s12890-015-0050-y.
-
Fujii, T., 2015. Dietary iron intake and hemoglobin concentration in college athletes in different sports. International Journal of Sports and Exercise Medicine 1: 029. https://doi.org/10.23937/2469-5718/1510029.
-
GoÌmez-Cambronero, J., 2001. The oxygen dissociation curve of hemoglobin: bridging the gap between biochemistry and physiology. Journal of Chemical Education 78: 757. https://doi.org/10.1021/ed078p757
-
Hargreaves, M. and Spriet, L.L., 2020. Skeletal muscle energy metabolism during exercise. Nature Metabolism 2: 817-828. https://doi.org/10.1038/s42255-020-0251-4.
-
Hawley, J.A. and Leckey, J.J., 2015. Carbohydrate dependence during prolonged, intense endurance exercise. Sports Medicine 45 (Suppl 1): S5-S12. https://doi.org/10.1007/s40279-015-0400-1.
-
Hendgen-Cotta, U.B., Kelm, M. and Rassaf, T., 2014. Myoglobin functions in the heart. Free Radical Biology and Medicine 73: 252-259. https://doi.org/10.1016/j.freeradbiomed.2014.05.005.
-
Kamga, C., Krishnamurthy, S. and Shiva, S., 2012. Myoglobin and mitochondria: a relationship bound by oxygen and nitric oxide. Nitric Oxide 26: 251-258. https://doi.org/10.1016/j.niox.2012.03.005.
-
Keller, P., Penkowa, M., Keller, C., Steensberg, A., Fischer, C.P., Giralt, M., Hidalgo, J. and Pedersen, B.K., 2005. Interleukin-6 receptor expression in contracting human skeletal muscle: regulating role of IL-6. FASEB Journal 19: 1181-1183. https://doi.org/10.1096/fj.04-3278fje.
-
Kenney, W.L., Wilmore, J.H. and Costill, D.L., 2015. Physiology of sport and exercise. 6th ed. Human Kinetics Publishers, Champaign, IL, USA.
-
Mauger, A.R., 2013. Fatigue is a pain â the use of novel neurophysiological techniques to understand the fatigue-pain relationship. Frontiers in Physiology 4: 104. https://doi.org/10.3389/fphys.2013.00104.
-
MunÌoz-CaÌnoves, P., Scheele, C., Pedersen, B.K. and Serrano, A.L., 2013. Interleukin-6 myokine signaling in skeletal muscle: a double-edged sword? FEBS Journal 280: 4131-4148. https://doi.org/10.1111/febs.12338.
-
Nemeth, P.M. and Lowry, O.H., 1984. Myoglobin levels in individual human skeletal muscle fibers of different types. Journal of Histochemistry and Cytochemistry 32: 1211-1216. https://doi.org/10.1177/32.11.6491255.
-
Ordway, G.A. and Garry, D.J., 2004. Myoglobin: an essential hemoprotein in striated muscle. Journal of Experimental Biology 207: 3441-3446. https://doi.org/10.1242/jeb.01172.
-
Pedersen, B.K., AÌkerström, T.C.A., Nielsen, A.R. and Fischer, C.P., 2007. Role of myokines in exercise and metabolism. Journal of Applied Physiology 103: 1093-1098. https://doi.org/10.1152/japplphysiol.00080.2007.
-
Pedersen, B.K., Steensberg, A. and Schjerling, P., 2001. Muscle-derived interleukin-6: possible biological effects. Journal of Physiology 536(Part 2): 329-337. https://doi.org/10.1111/j.1469-7793.2001.0329c.xd.
-
Petrosino, J.M., Heiss, V.J., Maurya, S.K., Kalyanasundaram, A., Periasamy, M., LaFountain, R.A., Wilson, J.M., Simonetti, O.P. and Ziouzenkova, O., 2016. Graded maximal exercise testing to assess mouse cardio-metabolic phenotypes. PLoS ONE 11: e0148010. https://doi.org/10.1371/journal.pone.0148010.
-
Pittman, R.N., 2011. Regulation of tissue oxygenation. Morgan & Claypool Life Sciences, San Rafael, CA, USA. Available at: https://www.ncbi.nlm.nih.gov/books/NBK54104/.
-
Postnikova, G.B. and Shekhovtsova, E.A., 2018. Myoglobin: oxygen depot or oxygen transporter to mitochondria? A novel mechanism of myoglobin deoxygenation in cells (review). Biochemistry 83: 168-183. https://doi.org/10.1134/S0006297918020098.
-
Rejeki, P.S., Utami, D.M., Izzatunnisa, N., Pranoto, A., Sukarno, D.A. and Fasitasari, M., 2021. A high-fat diet decreases serum TNF-alpha and breast tumor area on benzopyrene induced mice (Mus musculus). Chiang Mai University Journal of Natural Sciences 20: e2021089. https://doi.org/10.12982/CMUJNS.2021.089.
-
Reynolds, J.C. and Lee, C., 2020. Mouse fitness as determined through treadmill running and walking. Methods in Molecular Biology 2144: 57-65. https://doi.org/10.1007/978-1-0716-0592-9_5.
-
Saghiv, M.S. and Sagiv, M.S., 2020. Basic exercise physiology: clinical and laboratory perspectives. Springer International Publishing, Cham, Switzerland. https://doi.org/10.1007/978-3-030-48806-2.
-
Sherwood, L., 2016. Human physiology: from cells to systems. 9th ed. Cengage Learning, Boston, MA, USA.
-
SÌkof, L.S.B., 2013. Factors of success in endurance sports; changing of muscle fiber type. Acta Medica Medianae 52: 69-74. https://doi.org/10.5633/amm.2013.0413.
-
Steensberg, A., Hall, G., Osada, T., Sacchetti, M., Saltin, B. and Pedersen, B.K., 2000. Production of interleukin-6 in contracting human skeletal muscles can account for the exercise-induced increase in plasma interleukin-6. Journal of Physiology 529: 237-242. https://doi.org/10.1111/j.1469-7793.2000.00237.x.
-
Tanaka, T., Narazaki, M. and Kishimoto, T., 2014. IL-6 in inflammation, immunity and disease. Cold Spring Harbor Perspectives in Biology 6: a016295. https://doi.org/10.1101/cshperspect.a016295.
-
Van Der Zwaard, S., Brocherie, F. and Jaspers, R.T., 2021. Under the hood: skeletal muscle determinants of endurance performance. Frontiers in Sports and Active Living 3: 719434. https://doi.org/10.3389/fspor.2021.719434.
-
Wright, T.J. and Davis, R.W., 2015. Myoglobin extraction from mammalian skeletal muscle and oxygen affinity determination under physiological conditions. Protein Expression and Purification 107: 50-55. https://doi.org/10.1016/j.pep.2014.11.004.
-
Yamada, T., Furuichi, Y., Takakura, H., Hashimoto, T., Hanai, Y., Jue, T. and Masuda, K., 2013. Interaction between myoglobin and mitochondria in rat skeletal muscle. Journal of Applied Physiology 114: 490-497. https://doi.org/10.1152/japplphysiol.00789.2012.
| å ¨é¨æé´ | è¿å»ä¸å¹´ | è¿å»30天 | |
|---|---|---|---|
| æè¦æµè§æ¬¡æ° | 804 | 287 | 21 |
| å ¨ææµè§æ¬¡æ° | 14 | 2 | 0 |
| PDFä¸è½½æ¬¡æ° | 21 | 3 | 0 |
Single bout of a long-duration running treadmill increases myoglobin but not haemoglobin and interleukin 6 levels in mice (Mus musculus)
äºComparative Exercise PhysiologyDepartment of Health and Recreation Education, Faculty of Sport Science, Universitas Negeri Surabaya, Lidah Wetan Street, East Java 60213, Indonesia
Search for other papers by D. Yuliastrid in
Current site
Google Scholar
PubMed
Search for other papers by N.W. Kusnanik in
Current site
Google Scholar
PubMed
Search for other papers by B. Purwanto in
Current site
Google Scholar
PubMed
Search for other papers by A. Noordia in
Current site
Google Scholar
PubMed
Search for other papers by S.P. Purwoto in
Current site
Google Scholar
PubMed
Search for other papers by A. Pranoto in
Current site
Google Scholar
PubMed
Abstract
Endurance was essential to perform an activity for as long as possible. Previously, haemoglobin, myoglobin, lactate and interleukin (IL)-6 level played a key role in determining endurance. This study aims to analyse the differences in the duration of running a treadmill on the levels of haemoglobin, myoglobin, lactate, and IL-6 in mice (Mus musculus). The mice were forced to run on the treadmill for as long as possible. They were divided into 3 groups, namely Short (n = 12; â¤12.5 min treadmill running), Moderate (n = 12; 12.5-40 min treadmill running), and Long (n = 12; â¥40 min treadmill running). Haemoglobin and lactate levels were evaluated, respectively, using the Quick-check Plus Hb Haemoglobin Testing System and Accutrend® Plus system. Analysis of myoglobin and IL-6 levels used an enzyme-linked immunosorbent assay (ELISA) kit. Statistical analysis used the one-way ANOVA, and LSD post-hoc tests with a significance level of 5%. The results showed that only the myoglobin level characterised endurance. Higher myoglobin levels were found at longer duration of running. Haemoglobin, lactate and IL-6 levels did not differ between groups. Based on the results of the study it was concluded that a longer duration of running was found a higher level of myoglobin. Myoglobin but not haemoglobin represented as an essential characteristic for endurance among runners in mice. High-affinity binding between myoglobin and oxygen was essential for oxygen storage in muscle.
| å ¨é¨æé´ | è¿å»ä¸å¹´ | è¿å»30天 | |
|---|---|---|---|
| æè¦æµè§æ¬¡æ° | 804 | 287 | 21 |
| å ¨ææµè§æ¬¡æ° | 14 | 2 | 0 |
| PDFä¸è½½æ¬¡æ° | 21 | 3 | 0 |
