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Effect of voluntary wheel running on autophagy status in lung tissue of high-fat diet-fed rats

于Comparative Exercise Physiology
著者:
J. Rezaie Solid Tumor Research Center, Cellular and Molecular Medicine Institute, Urmia University of Medical Sciences, 5714783734 Urmia, Iran.

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A. Aboulhassani Student Research Committee, Tabriz University of Medical Sciences, 5165665931 Tabriz, Iran.
Department of Physiology, Faculty of Medicine, Tabriz University of Medical Sciences, 5165665931 Tabriz, Iran.

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R. Keyhanmanesh Drug Applied Research Center, Tabriz University of Medical Sciences, 5165665931 Tabriz, Iran.

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R. Rahbarghazi Stem Cell Research Center, Tabriz University of Medical Sciences, 5165665931 Tabriz, Iran.
Department of Applied Cell Sciences, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, 5165665931 Tabriz, Iran.

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A. Delkhosh Stem Cell Research Center, Tabriz University of Medical Sciences, 5165665931 Tabriz, Iran.

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L. Salimi Stem Cell Research Center, Tabriz University of Medical Sciences, 5165665931 Tabriz, Iran.

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A.R. Nezhad Zamani Stem Cell Research Center, Tabriz University of Medical Sciences, 5165665931 Tabriz, Iran.

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A. Rahbarghazi Department of Physical Education and Sports Sciences, Faculty of Educational Science and Psychology, University of Mohaghegh Ardabil, 56199-11367 Ardabil, Iran.

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M. Ahmadi Stem Cell Research Center, Tabriz University of Medical Sciences, 5165665931 Tabriz, Iran.

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F. Ghiasi Molecular Medicine Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.

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Here, we aimed to explore the therapeutic effect of voluntary wheel running (VWR) in high-fat diet-fed rats on pulmonary tissue injury via the modulation of autophagic response. Thirty-two rats were allocated into four groups; normal diet (Control); VWR; high-fat-diet (HFD), and HFD + VWR. After three months, pathological effect of HFD on pulmonary tissue was investigated. The levels of tumour necrosis factor (TNF)-α were detected in the bronchoalveolar lavage fluid (BALF). We monitored the expression of interleukin (IL)-6 and autophagy-related genes in lung tissues. H&E staining showed pathological changes in HFD group coincided with the increase of TNF-α levels in the bronchoalveolar fluid compared to the normal rats. Our results showed the up-regulation of IL-6, becline-1, LC3 and P62 in the HFD group compared to the Control group. VWR inhibited HFD-induced changes and could decrease HFD-induced changes via the regulation of autophagy status.

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