This study aimed to evaluate the effect of four weeks of aerobic exercise training combined with probiotic supplementation on mRNA levels of Nrf-2 and caspase-3 genes, superoxide dismutase (SOD), and serum total antioxidant capacity (TAC) in rats with type 2 diabetes. 40 male Wistar diabetic rats were divided into five groups: healthy placebo control group (NC), diabetic control group without supplement (DC), diabetic control group with supplement (SDC), diabetic aerobic training group without supplement (DT), and diabetic aerobic training group with probiotic supplement (SDT). Each training group performed training five days per week for four weeks and each session of training consisted of 30 min running on a treadmill with an intensity of 65-60% of maximum speed. Simultaneously, rats were fed probiotic supplements. Serum glucose, SOD, and TAC were analysed. The real-time PCR technique was used to determine the gene expression of Nrf-2 and caspase-3. Both aerobic exercise training and probiotic supplementation interactively reduced caspase 3 gene expression, increased Nrf-2 gene expression and enhanced TAC in the left ventricle of diabetic rats. Also, the reduction of caspase-3 mRNA in the left ventricle was more effective in the SDT group than in other diabetic groups. There was no interaction effect on SOD. However, a simultaneous effect of training and supplementation was observed on increasing TAC levels when compared to the DC group. Pearson’s correlation showed that the heart weight gain in the SDT group occurred only by decreasing the expression of the caspase-3 gene. Based on these results, probiotics combined with exercise training can be a strategy for improving the antioxidant defence system and preventing risk factors of diabetic cardiomyocytes, especially cell death and myocardial ischemia.
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| All Time | Past 365 days | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 372 | 163 | 38 |
| Full Text Views | 19 | 3 | 0 |
| PDF Views & Downloads | 39 | 10 | 0 |
This study aimed to evaluate the effect of four weeks of aerobic exercise training combined with probiotic supplementation on mRNA levels of Nrf-2 and caspase-3 genes, superoxide dismutase (SOD), and serum total antioxidant capacity (TAC) in rats with type 2 diabetes. 40 male Wistar diabetic rats were divided into five groups: healthy placebo control group (NC), diabetic control group without supplement (DC), diabetic control group with supplement (SDC), diabetic aerobic training group without supplement (DT), and diabetic aerobic training group with probiotic supplement (SDT). Each training group performed training five days per week for four weeks and each session of training consisted of 30 min running on a treadmill with an intensity of 65-60% of maximum speed. Simultaneously, rats were fed probiotic supplements. Serum glucose, SOD, and TAC were analysed. The real-time PCR technique was used to determine the gene expression of Nrf-2 and caspase-3. Both aerobic exercise training and probiotic supplementation interactively reduced caspase 3 gene expression, increased Nrf-2 gene expression and enhanced TAC in the left ventricle of diabetic rats. Also, the reduction of caspase-3 mRNA in the left ventricle was more effective in the SDT group than in other diabetic groups. There was no interaction effect on SOD. However, a simultaneous effect of training and supplementation was observed on increasing TAC levels when compared to the DC group. Pearson’s correlation showed that the heart weight gain in the SDT group occurred only by decreasing the expression of the caspase-3 gene. Based on these results, probiotics combined with exercise training can be a strategy for improving the antioxidant defence system and preventing risk factors of diabetic cardiomyocytes, especially cell death and myocardial ischemia.
| All Time | Past 365 days | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 372 | 163 | 38 |
| Full Text Views | 19 | 3 | 0 |
| PDF Views & Downloads | 39 | 10 | 0 |