The effects of acute strenuous exercise on the faecal microbiota in Standardbred racehorses
In: Comparative Exercise PhysiologyDepartment of Veterinary Internal Medicine, College of Veterinary Medicine, Al-Qadisiya University, Diwaniya, Iraq.
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While exercise has been found to change the faecal microbiome (FM) in laboratory animals exposed over weeks, no studies have identified immediate changes in the FM associated with short spans of intense exercise, ~5 min. The purpose of this study was to test the hypothesis that acute intense exercise would alter the FM in horses. Each horse performed two rounds of testing undergoing both a graded exercise test (GXT) and a parallel standing control (SC) trial before (GXT1 and SC1) and after (GXT2 and SC2) 12 weeks of exercise training. Rectal faecal samples were taken 24 h before and after testing. Bacterial community analysis was done by sequencing the 16s rRNA (V3-V4) region via Illumina Miseq. The relative abundance of the genus Clostridium significantly decreased in SC1 (P<0.05), with a concurrent decrease in the Shannon diversity index at the species level (P<0.05). At both the genus and species levels the principle coordinate analysis (PCoA) showed significant separation when the samples collected before SC1 were compared to those collected after SC1 (P<0.05). Interestingly, we found that Fusicatenibacter saccharivorans, a bacteria found to be decreased in ulcerative colitis patients, and Treponema zioleckii, a bacteria found to degrade fructan in sheep rumen, were significantly decreased when the samples collected before SC1 were compared to those collected after SC1 (P<0.05). None of the changes observed in SC1 happened in SC2 (P>0.05). Our results indicate that very intense acute exercise does not alter the faecal microbiome of the Standardbred race horse and that 12 weeks of exercise training does not alter that response.
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Bailey, M.T., Dowd, S.E., Galley, J.D., Hufnagle, A.R., Allen, R.G. and Lyte, M., 2011. Exposure to a social stressor alters the structure of the intestinal microbiota: implications for stressor-induced immunomodulation. Brain, Behavior and Immunity 25: 397-407.
'Exposure to a social stressor alters the structure of the intestinal microbiota: implications for stressor-induced immunomodulation ' () 25 Brain, Behavior and Immunity : 397 -407 .
-
Bjursell, M.K., Martens, E.C. and Gordon, J.I., 2006. Functional genomic and metabolic studies of the adaptations of a prominent adult human fecal symbiont, Bacteroides thetaiotaomicron, to the suckling period. Journal of Biological Chemistry 281: 36269-36279.
'Functional genomic and metabolic studies of the adaptations of a prominent adult human fecal symbiont, Bacteroides thetaiotaomicron, to the suckling period ' () 281 Journal of Biological Chemistry : 36269 -36279 .
-
Carabotti, M., Scirocco, A., Maselli, M.A. and Severi, C., 2015. The fecal-brain axis: interactions between enteric microbiota, central and enteric nervous systems. Annals of Gastroenterology 28: 203-209.
'The fecal-brain axis: interactions between enteric microbiota, central and enteric nervous systems ' () 28 Annals of Gastroenterology : 203 -209 .
-
Costa, M.C. and Weese, J.S., 2012. The equine intestinal microbiome. Animal Health Research Reviews 13: 121-128.
'The equine intestinal microbiome ' () 13 Animal Health Research Reviews : 121 -128 .
-
DeSantis, T.Z., Hugenholtz, P., Larsen, N.M.R., Brodie, E.L., Keller, K., Huber, T., Dalevi, D., Hu, P. and Andersen, G.L., 2006. Greengenes, a chimera-checked 16S rRNA gene database and workbench compatible with ARB. Applied and Environmental Microbiology 72: 5069-5072.
'Greengenes, a chimera-checked 16S rRNA gene database and workbench compatible with ARB ' () 72 Applied and Environmental Microbiology : 5069 -5072 .
-
Desvaux, M., 2005. Clostridium cellulolyticum: model organism of mesophilic cellulolytic clostridia. FEMS Microbiology Reviews 29: 741-764.
'Clostridium cellulolyticum: model organism of mesophilic cellulolytic clostridia ' () 29 FEMS Microbiology Reviews : 741 -764 .
-
Evans, C.C., LePard, K.J., Kwak, J.W., Stancukas, M.C., Laskowski, S., Dougherty, J., Moulton, L., Glawe, A., Wang, Y., Leone, V., Antonopoulos, D.A., Smith, D., Chang, E.B. and Ciancio, M.J., 2014. Exercise prevents weight gain and alters the FM in a mouse model of high fat diet-induced obesity. PLoS ONE 9: e92193.
'Exercise prevents weight gain and alters the FM in a mouse model of high fat diet-induced obesity ' () 9 PLoS ONE : e92193 .
-
Evans, D.G., Miles A.A. and Niven J.S., 1948. The enhancement of bacterial infections by adrenaline. British Journal Experimental Pathology 29: 20-39.
'The enhancement of bacterial infections by adrenaline ' () 29 British Journal Experimental Pathology : 20 -39 .
-
Foster, J.A., 2013. Fecal feelings: bacteria and the brain. Cerebrum 2013: 9. Freestone, P., 2013. Communication between bacteria and their hosts. Scientifica 2013: 361073.
'Fecal feelings: bacteria and the brain ' () 2013 Scientifica : 361073 .
-
Gordon, M.E., McKeever, K.H., Betros, C.L. and Manso Filho, H.C., 2007. Exercise-induced alterations in plasma concentrations of ghrelin, adiponectin, leptin, glucose, insulin, and cortisol in horses. Veterinary Journal 173: 532-540.
'Exercise-induced alterations in plasma concentrations of ghrelin, adiponectin, leptin, glucose, insulin, and cortisol in horses ' () 173 Veterinary Journal : 532 -540 .
-
Hethener, P., Brauman, A. and Garcia, J.L., 1992. Clostridium termitidis sp. nov., a cellulolytic bacterium from the fecal of the wood-feeding termite, Nasutitermes lujae. Systematic and Applied Microbiology 15: 52-58.
'Clostridium termitidis sp ' () 15 Systematic and Applied Microbiology : 52 -58 .
-
Janabi, A.H.D., Biddle, A.S., Klein, D.S. and McKeever, K.H., 2016a. Exercise training changes the gut microbiota of Standardbred racehorses. Comparative Exercise Physiology 12: 119-129.
'Exercise training changes the gut microbiota of Standardbred racehorses ' () 12 Comparative Exercise Physiology : 119 -129 .
-
Janabi, A.H.D., Kerkhof, L.J., McGuinness, L.R., Biddle, A.S. and McKeever, K.H., 2016b. Comparison of a modified phenol/chloroform and commercial-kit methods for extracting DNA from horse fecal material. Journal of Microbiological Methods 129: 14-19.
'Comparison of a modified phenol/chloroform and commercial-kit methods for extracting DNA from horse fecal material ' () 129 Journal of Microbiological Methods : 14 -19 .
-
Kearns, C.F. and McKeever, K.H., 2002. Clenbuterol diminishes aerobic performance in horses. Medicine and Science in Sport and Exercise 34: 1976-1985.
'Clenbuterol diminishes aerobic performance in horses ' () 34 Medicine and Science in Sport and Exercise : 1976 -1985 .
-
Liu, T.W., Park, Y.M., Holscher, H.D., Padilla, J., Scroggins, R.J., Welly, R., Britton, S.L., Koch, L.G., Vieira-Potter, V.J. and Swanson, K.S., 2015. Physical activity differentially affects the cecal microbiota of ovariectomized female rats selectively bred for high and low aerobic capacity. PLoS ONE 10: e0136150.
'Physical activity differentially affects the cecal microbiota of ovariectomized female rats selectively bred for high and low aerobic capacity ' () 10 PLoS ONE : e0136150 .
-
Mackie, R.I., Sghir, A. and Gaskins, H.R., 1999. Developmental microbial ecology of the neonatal gastrointestinal tract. American Journal of Clinical Nutrition 69: 1035S-1045S.
'Developmental microbial ecology of the neonatal gastrointestinal tract ' () 69 American Journal of Clinical Nutrition : 1035S -1045S .
-
McKeever, K.H. and Lehnhard, R.A., 2014. Physiology of acid base balance and fluid shifts with exercise. In: Hodgson, D.R., McKeever, K.H. and McGowan, C.T. (eds.) The athletic horse, principles and practice of equine sports medicine, 2nd ed. Elsevier, St. Louis, MO, USA, pp. 69-87.
'Physiology of acid base balance and fluid shifts with exercise ', in The athletic horse, principles and practice of equine sports medicine , () 69 -87 .
-
Mika, A., Van Treuren, W., González, A., Herrera, J.J., Knight, R. and Fleshner, M., 2015. Exercise is more effective at altering fecal microbial composition and producing stable changes in lean mass in juvenile versus adult male F344 rats. PLoS ONE 10: e0125889.
'Exercise is more effective at altering fecal microbial composition and producing stable changes in lean mass in juvenile versus adult male F344 rats ' () 10 PLoS ONE : e0125889 .
-
Mühling, M., Woolven-Allen, J., Murrell, J.C. and Joint, I., 2008. Improved group-specific PCR primers for denaturing gradient gel electrophoresis analysis of the genetic diversity of complex microbial communities. International Society for Microbial Ecology Journal 2: 379-392.
'Improved group-specific PCR primers for denaturing gradient gel electrophoresis analysis of the genetic diversity of complex microbial communities ' () 2 International Society for Microbial Ecology Journal : 379 -392 .
-
Oksanen, J., Blanchet, F.G., Kindt, R., Legendre, P., Minchin, P.R., O’Hara, R.B., Simpson, G.L., Solymos, P., Henry, M., Stevens, H. and Wagner, H., 2016. Vegan: community ecology package. R package version 2.3-3. Available at: https://CRAN.R-project.org/package=vegan.
-
Potera, C., 2013. Running interference? Exercise and PCB-induced changes in the fecal microbiome. Environmental Health Perspectives 121: A199.
'Running interference? Exercise and PCB-induced changes in the fecal microbiome ' () 121 Environmental Health Perspectives : A199 .
-
Queipo-Ortuño, M.I., Seoane, L.M., Murri, M., Pardo, M., Gomez-Zumaquero, J.M., Cardona, F., Casanueva, F. and Tinahones, F.J., 2013. FM composition in male rat models under different nutritional status and physical activity and its association with serum leptin and ghrelin levels. PLoS ONE 8: e65465.
'FM composition in male rat models under different nutritional status and physical activity and its association with serum leptin and ghrelin levels ' () 8 PLoS ONE : e65465 .
-
Rowell, L.B., 1983. Cardiovascular adjustments to thermal stress. In: Shepherd, J.T., Abboud, F.M. and Geiger, S.R. (eds.) Handbook of physiology. The cardiovascular system. Peripheral circulation and organ blood flow. Vol. 11. American Physiology Society, Bethesda, MD, USA, pp. 967-1023.
'Cardiovascular adjustments to thermal stress ', in Handbook of physiology. The cardiovascular system. Peripheral circulation and organ blood flow , () 967 -1023 .
-
Santacruz, A., Marcos, A., Wärnberg, J., Martí, A., Martin-Matillas, M., Campoy, C., Moreno, L.A., Veiga, O., Redondo-Figuero, C., Garagorri, J.M., Azcona, C., Delgado, M., García-Fuentes, M., Collado, M.C., Sanz, Y. and the EVASYON Study Group, 2009. Interplay between weight loss and FM composition in overweight adolescents. Obesity 17: 1906-1915.
'Interplay between weight loss and FM composition in overweight adolescents ' () 17 Obesity : 1906 -1915 .
-
Smith-Slatas, C.L., Bourque, M. and Salazar, J.C., 2006. Clostridium septicum infections in children: a case report and review of the literature. Pediatrics 117: e796-805.
'Clostridium septicum infections in children: a case report and review of the literature ' () 117 Pediatrics : e796 -805 .
-
Tannock, G.W. and Savage, D.C., 1974. Influences of dietary and environmental stress on microbial populations in the murine gastrointestinal tract. Infection and Immunity 9: 591-598.
'Influences of dietary and environmental stress on microbial populations in the murine gastrointestinal tract ' () 9 Infection and Immunity : 591 -598 .
-
The GIMP team, 1997-2014. GIMP 2.8.10. Available at: www.gimp.org.
-
Venables, W.N. and Ripley, B.D., 2002. Modern applied statistics with S, 4th ed. Springer, New York, NY, USA.
'Modern applied statistics with S, 4th ed ', ().
-
Yan, Q., Van der Gast, C.J. and Yu, Y., 2012. Bacterial community assembly and turnover within the intestines of developing zebrafish. PLoS ONE 7: e30603.
'Bacterial community assembly and turnover within the intestines of developing zebrafish ' () 7 PLoS ONE : e30603 .
-
Zhang, M., Liu, N., Qian, C., Wang, Q., Wang, Q., Long, Y., Huang, Y., Zhou, Z. and Yan, X., 2014. Phylogenetic and functional analysis of fecal microbiota of a fungus-growing higher termite: Bacteroidetes from higher termites are a rich source of β-glucosidase genes. Microbial Ecology 68: 416-425.
'Phylogenetic and functional analysis of fecal microbiota of a fungus-growing higher termite: Bacteroidetes from higher termites are a rich source of β-glucosidase genes ' () 68 Microbial Ecology : 416 -425 .
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The effects of acute strenuous exercise on the faecal microbiota in Standardbred racehorses
In: Comparative Exercise PhysiologyDepartment of Veterinary Internal Medicine, College of Veterinary Medicine, Al-Qadisiya University, Diwaniya, Iraq.
Search for other papers by A.H.D. Janabi in
Current site
Google Scholar
PubMed
Search for other papers by A.S. Biddle in
Current site
Google Scholar
PubMed
Search for other papers by D.J. Klein in
Current site
Google Scholar
PubMed
Search for other papers by K.H. McKeever in
Current site
Google Scholar
PubMed
While exercise has been found to change the faecal microbiome (FM) in laboratory animals exposed over weeks, no studies have identified immediate changes in the FM associated with short spans of intense exercise, ~5 min. The purpose of this study was to test the hypothesis that acute intense exercise would alter the FM in horses. Each horse performed two rounds of testing undergoing both a graded exercise test (GXT) and a parallel standing control (SC) trial before (GXT1 and SC1) and after (GXT2 and SC2) 12 weeks of exercise training. Rectal faecal samples were taken 24 h before and after testing. Bacterial community analysis was done by sequencing the 16s rRNA (V3-V4) region via Illumina Miseq. The relative abundance of the genus Clostridium significantly decreased in SC1 (P<0.05), with a concurrent decrease in the Shannon diversity index at the species level (P<0.05). At both the genus and species levels the principle coordinate analysis (PCoA) showed significant separation when the samples collected before SC1 were compared to those collected after SC1 (P<0.05). Interestingly, we found that Fusicatenibacter saccharivorans, a bacteria found to be decreased in ulcerative colitis patients, and Treponema zioleckii, a bacteria found to degrade fructan in sheep rumen, were significantly decreased when the samples collected before SC1 were compared to those collected after SC1 (P<0.05). None of the changes observed in SC1 happened in SC2 (P>0.05). Our results indicate that very intense acute exercise does not alter the faecal microbiome of the Standardbred race horse and that 12 weeks of exercise training does not alter that response.
| All Time | Past 365 days | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 449 | 187 | 20 |
| Full Text Views | 12 | 2 | 0 |
| PDF Views & Downloads | 19 | 5 | 0 |
