Evaluation of antioxidant activity of novel potential probiotic cocktail in mice model of gut inflammation via oxidative stress-related pathways
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Abstract
Oxidative stress plays a key role in colitis, a type of Inflammatory Bowel Disease, particularly when associated with a high-fat diet (HFD). Probiotics are known to alleviate inflammation through multiple mechanisms. This study aimed to evaluate the efficacy of a potential probiotic mixture with high antioxidant activity in attenuating colitis in mice fed either a normal diet (ND) or an HFD, with a focus on oxidative stress–related pathways. Eighty-eight Lactobacillus and Bifidobacterium strains isolated from healthy human faeces and milk were screened for antioxidant capacity. The six most active strains were selected to formulate a probiotic cocktail. Male C57BL/6 mice were divided into ND and HFD groups, each receiving dextran sulphate sodium (DSS) alone or combined with the probiotic cocktail. Disease indices, histopathology, and the expression of genes related to NF-kB and Nrf2 signalling, as well as oxidative and inflammatory markers, were assessed. Mice treated with the probiotic cocktail showed significant attenuation of DSS-induced colitis, evidenced by lower Disease Activity Index and pathological scores, and improved intestinal morphology (
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Supplementary Materials
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Evaluation of antioxidant activity of novel potential probiotic cocktail in mice model of gut inflammation via oxidative stress-related pathways
In: Beneficial MicrobesSearch for other papers by N. Rezaie in
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PubMed
Search for other papers by S. Aghamohammad in
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PubMed
Search for other papers by F. Bagheri-Amiri in
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Search for other papers by M. Talebi in
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Search for other papers by A. Sohrabi in
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Search for other papers by M.R. Pourshafie in
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Search for other papers by M. Rohani in
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PubMed
Abstract
Oxidative stress plays a key role in colitis, a type of Inflammatory Bowel Disease, particularly when associated with a high-fat diet (HFD). Probiotics are known to alleviate inflammation through multiple mechanisms. This study aimed to evaluate the efficacy of a potential probiotic mixture with high antioxidant activity in attenuating colitis in mice fed either a normal diet (ND) or an HFD, with a focus on oxidative stress–related pathways. Eighty-eight Lactobacillus and Bifidobacterium strains isolated from healthy human faeces and milk were screened for antioxidant capacity. The six most active strains were selected to formulate a probiotic cocktail. Male C57BL/6 mice were divided into ND and HFD groups, each receiving dextran sulphate sodium (DSS) alone or combined with the probiotic cocktail. Disease indices, histopathology, and the expression of genes related to NF-kB and Nrf2 signalling, as well as oxidative and inflammatory markers, were assessed. Mice treated with the probiotic cocktail showed significant attenuation of DSS-induced colitis, evidenced by lower Disease Activity Index and pathological scores, and improved intestinal morphology (
| All Time | Past 365 days | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 150 | 150 | 24 |
| Full Text Views | 2 | 2 | 0 |
| PDF Views & Downloads | 2 | 2 | 0 |
