Lactobacillus casei BL23 modulates the innate immune response in Staphylococcus aureus-stimulated bovine mammary epithelial cells
In: Beneficial MicrobesAgrocampus Ouest, UMR 1253 STLO, 85 Rue de Saint Brieuc, 35042 Rennes, France.
Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, 31270-901 MG, Brazil.
Universidade Federal do Vale do São Francisco, Av. José de Sá Maniçoba, S/N Centro, Petrolina, 56304-917 PE, Brazil.
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Agrocampus Ouest, UMR 1253 STLO, 85 Rue de Saint Brieuc, 35042 Rennes, France.
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Agrocampus Ouest, UMR 1253 STLO, 85 Rue de Saint Brieuc, 35042 Rennes, France.
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Agrocampus Ouest, UMR 1253 STLO, 85 Rue de Saint Brieuc, 35042 Rennes, France.
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Probiotics have been adopted to treat and prevent various diseases in humans and animals. They were notably shown to be a promising alternative to prevent mastitis in dairy cattle. This inflammation of the mammary gland is generally of infectious origin and generates extensive economic losses worldwide. In a previous study, we found that Lactobacillus casei BL23 was able to inhibit the internalisation of Staphylococcus aureus, one of the major pathogens involved in mastitis, into bovine mammary epithelial cells (bMEC). In this study, we further explored the capacity of this strain to modulate the innate immune response of bovine mammary epithelial cells during S. aureus infection. L. casei BL23 was able to decrease the expression of several pro-inflammatory cytokines, including interleukins 6, 8, 1α and 1β and tumour necrosis factor alpha, in S. aureus-stimulated bMEC, 8 h post-infection. On the other hand, L. casei did not impair the induction of defensins, such as lingual antimicrobial peptide and defensin β1 in the presence of S. aureus, and even slightly increased the induction of tracheal antimicrobial peptide during S. aureus infection. Finally, this strain did not alter the expression of the pattern recognition receptor nucleotide-binding oligomerisation domain proteins (NOD2). This study demonstrates that L. casei BL23 displayed anti-inflammatory properties on S. aureus-stimulated bMEC. These results open the way to further characterisation of the BL23 probiotic potential in a bovine mammary gland context and to a better understanding of how all these beneficial properties combine in vivo to combat mastitis pathogens.
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Aitken, S.L., Corl, C.M. and Sordillo, L.M., 2011. Immunopathology of mastitis: insights into disease recognition and resolution. Journal of Mammary Gland Biology and Neoplasia 16: 291-304.
'Immunopathology of mastitis: insights into disease recognition and resolution ' () 16 Journal of Mammary Gland Biology and Neoplasia : 291 -304.
-
Akira, S., Takeda, K. and Kaisho, T., 2001. Toll-like receptors: critical proteins linking innate and acquired immunity. Nature Immunology 2: 675-680.
'Toll-like receptors: critical proteins linking innate and acquired immunity ' () 2 Nature Immunology : 675 -680.
-
Amara, A.A. and Shibl, A., 2015. Role of probiotics in health improvement, infection control and disease treatment and management. Saudi Pharmaceutical Journal 23: 107-114.
'Role of probiotics in health improvement, infection control and disease treatment and management ' () 23 Saudi Pharmaceutical Journal : 107 -114.
-
Armas, F., Camperio, C. and Marianelli, C., 2017. In vitro assessment of the probiotic potential of Lactococcus lactis LMG 7930 against ruminant mastitis-causing pathogens. PLoS ONE 12: e0169543.
'In vitro assessment of the probiotic potential of Lactococcus lactis LMG 7930 against ruminant mastitis-causing pathogens ' () 12 PLoS ONE : e0169543.
-
Bannerman, D.D., Paape, M.J., Lee, J.W., Zhao, X., Hope, J.C. and Rainard, P., 2004. Escherichia coli and Staphylococcus aureus elicit differential innate immune responses following intramammary infection. Clinical and Diagnostic Laboratory Immunology 11: 463-472.
'Escherichia coli and Staphylococcus aureus elicit differential innate immune responses following intramammary infection ' () 11 Clinical and Diagnostic Laboratory Immunology : 463 -472.
-
Baron, F., Cochet, M.F., Ablain, W., Grosset, N., Madec, M.N., Gonnet, F., Jan, S. and Gautier, M., 2006. Rapid and cost-effective method for microorganism enumeration based on miniaturization of the conventional plate-counting technique. Lait 86: 251-257.
'Rapid and cost-effective method for microorganism enumeration based on miniaturization of the conventional plate-counting technique ' () 86 Lait : 251 -257.
-
Bermudez-Brito, M., Plaza-Diaz, J., Munoz-Quezada, S., Gomez-Llorente, C. and Gil, A., 2012. Probiotic mechanisms of action. Annals of Nutrition and Metabolism 61: 160-174.
'Probiotic mechanisms of action ' () 61 Annals of Nutrition and Metabolism : 160 -174.
-
Beutler, B., 2003. How we detect microbes and respond to them: the toll-like receptors and their transducers. Journal of Leukocyte Biology 74: 479-485.
'How we detect microbes and respond to them: the toll-like receptors and their transducers ' () 74 Journal of Leukocyte Biology : 479 -485.
-
Bouchard, D.S., Rault, L., Berkova, N., Le Loir, Y. and Even, S., 2013. Inhibition of Staphylococcus aureus invasion into bovine mammary epithelial cells by contact with live Lactobacillus casei. Applied and Environmental Microbiology 79: 877-885.
'Inhibition of Staphylococcus aureus invasion into bovine mammary epithelial cells by contact with live Lactobacillus casei ' () 79 Applied and Environmental Microbiology : 877 -885.
-
Bouchard, D.S., Seridan, B., Saraoui, T., Rault, L., Germon, P., Gonzalez-Moreno, C., Nader-Macias, F.M.E., Baud, D., François, P., Chuat, V., Chain, F., Langella, P., Nicoli, J., Le Loir, Y. and Even, S., 2015. Lactic acid bacteria isolated from bovine mammary microbiota: potential allies against bovine mastitis. PLoS ONE 10: e0144831.
'Lactic acid bacteria isolated from bovine mammary microbiota: potential allies against bovine mastitis ' () 10 PLoS ONE : e0144831.
-
Bougarn, S., Cunha, P., Gilbert, F.B., Harmache, A., Foucras, G. and Rainard, P., 2011. Staphylococcal-associated molecular patterns enhance expression of immune defense genes induced by IL-17 in mammary epithelial cells. Cytokine 56: 749-759.
'Staphylococcal-associated molecular patterns enhance expression of immune defense genes induced by IL-17 in mammary epithelial cells ' () 56 Cytokine : 749 -759.
-
Crispie, F., Alonso-Gomez, M., O’Loughlin, C., Klostermann, K., Flynn, J., Arkins, S., Meaney, W., Paul, R.R. and Hill, C., 2008. Intramammary infusion of a live culture for treatment of bovine mastitis: effect of live lactococci on the mammary immune response. Journal of Dairy Research 75: 374-384.
'Intramammary infusion of a live culture for treatment of bovine mastitis: effect of live lactococci on the mammary immune response ' () 75 Journal of Dairy Research : 374 -384.
-
Deplanche, M., Alekseeva, L., Semenovskaya, K., Fu, C.-L., Dessauge, F., Finot, L., Petzl, W., Zerbe, H., Le Loir, Y., Rainard, P., Smith, D.G.E., Germon, P., Otto, M. and Berkova, N., 2016. Staphylococcus aureus phenol-soluble modulins impair interleukin expression in bovine mammary epithelial cells. Infection and Immunity 84: 1682-1692.
'Staphylococcus aureus phenol-soluble modulins impair interleukin expression in bovine mammary epithelial cells ' () 84 Infection and Immunity : 1682 -1692.
-
Dustin, M.L., Rothlein, R., Bhan, A.K., Dinarello, C.A. and Springer, T.A., 2011. Induction by IL 1 and interferon-γ: tissue distribution, biochemistry, and function of a natural adherence molecule (ICAM-1). Journal of Immunology 137: 245-254.
'Induction by IL 1 and interferon-γ: tissue distribution, biochemistry, and function of a natural adherence molecule (ICAM-1) ' () 137 Journal of Immunology : 245 -254.
-
Even, S., Bouchard, D. and Le Loir, Y., 2014. Lactic acid bacteria to modulate virulence expression in pathogenic bacteria: an alternative to killing? In: Pessione, E. (eds.) Interactive probiotics. CRC Press, Boca Raton, FL, USA, pp. 52-80.
'Lactic acid bacteria to modulate virulence expression in pathogenic bacteria: an alternative to killing? ', in Interactive probiotics , () 52 -80.
-
Evrard, B., Coudeyras, S., Dosgilbert, A., Charbonnel, N., Alamé, J., Tridon, A. and Forestier, C., 2011. Dose-dependent immunomodulation of human dendritic cells by the probiotic Lactobacillus rhamnosus Lcr35. PLoS ONE 6: e18735.
'Dose-dependent immunomodulation of human dendritic cells by the probiotic Lactobacillus rhamnosus Lcr35 ' () 6 PLoS ONE : e18735.
-
Foligne, B., Nutten, S., Grangette, C., Dennin, V., Goudercourt, D., Poiret, S., Dewulf, J., Brassart, D., Mercenier, A. and Pot, B., 2007. Correlation between in vitro and in vivo immunomodulatory properties of lactic acid bacteria. World Journal of Gastroenterology 13: 236-243.
'Correlation between in vitro and in vivo immunomodulatory properties of lactic acid bacteria ' () 13 World Journal of Gastroenterology : 236 -243.
-
Francoz, D., Wellemans, V., Dupré, J.P., Roy, J.P., Labelle, F., Lacasse, P. and Dufour, S., 2017. A systematic review and qualitative analysis of treatments other than conventional antimicrobials for clinical mastitis in dairy cows. Journal of Dairy Science 100: 7751-7770.
'A systematic review and qualitative analysis of treatments other than conventional antimicrobials for clinical mastitis in dairy cows ' () 100 Journal of Dairy Science : 7751 -7770.
-
Frola, I.D., Pellegrino, M.S., Espeche, M.C., Giraudo, J.A., Nader-Macias, M.E. and Bogni, C.I., 2012. Effects of intramammary inoculation of Lactobacillus perolens CRL1724 in lactating cows’ udders. Journal of Dairy Research 79: 84-92.
'Effects of intramammary inoculation of Lactobacillus perolens CRL1724 in lactating cows’ udders ' () 79 Journal of Dairy Research : 84 -92.
-
Fu, M., Chen, Y., Xiong, X., Lan, D. and Li, J., 2014. Establishment of mammary gland model in vitro: culture and evaluation of a yak mammary epithelial cell line. PLoS ONE 9: e113669.
'Establishment of mammary gland model in vitro: culture and evaluation of a yak mammary epithelial cell line ' () 9 PLoS ONE : e113669.
-
Fu, Y., Zhou, E., Liu, Z., Li, F., Liang, D., Liu, B., Song, X., Zhao, F., Fen, X., Li, D., Cao, Y., Zhang, X., Zhang, N. and Yang, Z., 2013. Staphylococcus aureus and Escherichia coli elicit different innate immune responses from bovine mammary epithelial cells. Veterinary Immunology and Immunopathology 155: 245-252.
'Staphylococcus aureus and Escherichia coli elicit different innate immune responses from bovine mammary epithelial cells ' () 155 Veterinary Immunology and Immunopathology : 245 -252.
-
Gärtner, M.A., Bondzio, A., Braun, N., Jung, M., Einspanier, R. and Gabler, C., 2015. Detection and characterisation of Lactobacillus spp. in the bovine uterus and their influence on bovine endometrial epithelial cells in vitro. PLoS ONE 10: e0119793.
'Detection and characterisation of Lactobacillus spp. in the bovine uterus and their influence on bovine endometrial epithelial cells in vitro ' () 10 PLoS ONE : e0119793.
-
Gilbert, F.B., Cunha, P., Jensen, K., Glass, E.J., Foucras, G., Robert-Granie, C., Rupp, R. and Rainard, P., 2013. Differential response of bovine mammary epithelial cells to Staphylococcus aureus or Escherichia coli agonists of the innate immune system. Veterinary Research 44: 40.
'Differential response of bovine mammary epithelial cells to Staphylococcus aureus or Escherichia coli agonists of the innate immune system ' () 44 Veterinary Research : 40.
-
Goldammer, T., Zerbe, H., Molenaar, A., Schuberth, H.-J., Brunner, R.M., Kata, S.R. and Seyfert, H.-M., 2004. Mastitis increases mammary mRNA abundance of -defensin 5, toll-like-receptor 2 (TLR2), and TLR4 but not TLR9 in cattle. Clinical and Vaccine Immunology 11: 174-185.
'Mastitis increases mammary mRNA abundance of -defensin 5, toll-like-receptor 2 (TLR2), and TLR4 but not TLR9 in cattle ' () 11 Clinical and Vaccine Immunology : 174 -185.
-
Günther, J., Petzl, W., Bauer, I., Ponsuksili, S., Zerbe, H., Schuberth, H.-J., Brunner, R.M. and Seyfert, H.-M., 2017. Differentiating Staphylococcus aureus from Escherichia coli mastitis: S. aureus triggers unbalanced immune-dampening and host cell invasion immediately after udder infection. Scientific Reports 7: 4811.
'Differentiating Staphylococcus aureus from Escherichia coli mastitis: S. aureus triggers unbalanced immune-dampening and host cell invasion immediately after udder infection ' () 7 Scientific Reports : 4811.
-
Hogeveen, H., Huijps, K. and Lam, T., 2011. Economic aspects of mastitis: new developments. New Zealand Veterinary Journal 59: 16-23.
'Economic aspects of mastitis: new developments ' () 59 New Zealand Veterinary Journal : 16 -23.
-
Junjua, M., Kechaou, N., Chain, F., Awussi, A.A., Roussel, Y., Perrin, C., Roux, E., Langella, P., Bermúdez-Humarán, L.G., Le Roux, Y., Chatel, J.-M. and Dary-Mourot, A., 2016. A large scale in vitro screening of Streptococcus thermophilus strains revealed strains with a high anti-inflammatory potential. LWT – Food Science and Technology 70: 78-87.
'A large scale in vitro screening of Streptococcus thermophilus strains revealed strains with a high anti-inflammatory potential ' () 70 LWT – Food Science and Technology : 78 -87.
-
Khani, S., Hosseini, H., Taheri, M., Nourani, M. and Imani Fooladi, A., 2012. Probiotics as an alternative strategy for prevention and treatment of human diseases: a review. Inflammation & Allergy-Drug Targets 11: 79-89.
'Probiotics as an alternative strategy for prevention and treatment of human diseases: a review ' () 11 Inflammation & Allergy-Drug Targets : 79 -89.
-
Klostermann, K., Crispie, F., Flynn, J., Ross, R.P., Hill, C. and Meaney, W., 2008. Intramammary infusion of a live culture of Lactococcus lactis for treatment of bovine mastitis: comparison with antibiotic treatment in field trials. Journal of Dairy Research 75: 365-373.
'Intramammary infusion of a live culture of Lactococcus lactis for treatment of bovine mastitis: comparison with antibiotic treatment in field trials ' () 75 Journal of Dairy Research : 365 -373.
-
Lahouassa, H., Moussay, E., Rainard, P. and Riollet, C., 2007. Differential cytokine and chemokine responses of bovine mammary epithelial cells to Staphylococcus aureus and Escherichia coli. Cytokine 38: 12-21.
'Differential cytokine and chemokine responses of bovine mammary epithelial cells to Staphylococcus aureus and Escherichia coli ' () 38 Cytokine : 12 -21.
-
Lee, B., Yin, X., Griffey, S.M. and Marco, M.L., 2015. Attenuation of colitis by Lactobacillus casei BL23 is dependent on the dairy delivery matrix. Applied and Environmental Microbiology 81: 6425-6435.
'Attenuation of colitis by Lactobacillus casei BL23 is dependent on the dairy delivery matrix ' () 81 Applied and Environmental Microbiology : 6425 -6435.
-
Lenoir, M., Del Carmen, S., Cortes-Perez, N.G., Lozano-Ojalvo, D., Muñoz-Provencio, D., Chain, F., Langella, P., De Moreno de LeBlanc, A., LeBlanc, J.G. and Bermúdez-Humarán, L.G., 2016. Lactobacillus casei BL23 regulates Treg and Th17 T-cell populations and reduces DMH-associated colorectal cancer. Journal of Gastroenterology 51: 862-873.
'Lactobacillus casei BL23 regulates Treg and Th17 T-cell populations and reduces DMH-associated colorectal cancer ' () 51 Journal of Gastroenterology : 862 -873.
-
Ljungh, A. and Wadström, T., 2006. Lactic acid bacteria as probiotics. Current Issues in Intestinal Microbiology 7: 73-89.
'Lactic acid bacteria as probiotics ' () 7 Current Issues in Intestinal Microbiology : 73 -89.
-
Malvisi, M., Stuknytė, M., Magro, G., Minozzi, G., Giardini, A., De Noni, I. and Piccinini, R., 2016. Antibacterial activity and immunomodulatory effects on a bovine mammary epithelial cell line exerted by nisin A-producing Lactococcus lactis strains. Journal of Dairy Science 99(3): 2288-2296.
'Antibacterial activity and immunomodulatory effects on a bovine mammary epithelial cell line exerted by nisin A-producing Lactococcus lactis strains ' () 99 Journal of Dairy Science : 2288 -2296.
-
Mazé, A., Boël, G., Zúñiga, M., Bourand, A., Loux, V., Yebra, M.J., Monedero, V., Correia, K., Jacques, N., Beaufils, S., Poncet, S., Joyet, P., Milohanic, E., Casarégola, S., Auffray, Y., Pérez-Martínez, G., Gibrat, J.-F., Zagorec, M., Francke, C., Hartke, A. and Deutscher, J., 2010. Complete genome sequence of the probiotic Lactobacillus casei strain BL23. Journal of Bacteriology 192: 2647-2648.
'Complete genome sequence of the probiotic Lactobacillus casei strain BL23 ' () 192 Journal of Bacteriology : 2647 -2648.
-
Menzies, B.E. and Kenoyer, A., 2005. Staphylococcus aureus infection of epidermal keratinocytes promotes expression of innate antimicrobial peptides. Infection and Immunity 73: 5241-5244.
'Staphylococcus aureus infection of epidermal keratinocytes promotes expression of innate antimicrobial peptides ' () 73 Infection and Immunity : 5241 -5244.
-
Nader-Macias, M.E., Otero, M.C., Espeche, M.C. and Maldonado, N.C., 2008. Advances in the design of probiotic products for the prevention of major diseases in dairy cattle. Journal of Industrial Microbiology and Biotechnology 35: 1387-1395.
'Advances in the design of probiotic products for the prevention of major diseases in dairy cattle ' () 35 Journal of Industrial Microbiology and Biotechnology : 1387 -1395.
-
Oviedo-Boyso, J., Valdez-Alarcón, J.J., Cajero-Juárez, M., Ochoa-Zarzosa, A., López-Meza, J.E., Bravo-Patiño, A. and Baizabal-Aguirre, V.M., 2007. Innate immune response of bovine mammary gland to pathogenic bacteria responsible for mastitis. Journal of Infection 54: 399-409.
'Innate immune response of bovine mammary gland to pathogenic bacteria responsible for mastitis ' () 54 Journal of Infection : 399 -409.
-
Pellegrino, M., Berardo, N., Giraudo, J., Nader-Macías, M.E.F. and Bogni, C., 2017. Bovine mastitis prevention: humoral and cellular response of dairy cows inoculated with lactic acid bacteria at the dry-off period. Beneficial Microbes 8: 589-596.
'Bovine mastitis prevention: humoral and cellular response of dairy cows inoculated with lactic acid bacteria at the dry-off period ' () 8 Beneficial Microbes : 589 -596.
-
Peton, V. and Le Loir, Y., 2013. Staphylococcus aureus in veterinary medicine. Infection, Genetics and Evolution 21: 602-615.
'Staphylococcus aureus in veterinary medicine ' () 21 Infection, Genetics and Evolution : 602 -615.
-
Porcherie, A., Cunha, P., Trotereau, A., Roussel, P., Gilbert, F.B., Rainard, P. and Germon, P., 2012. Repertoire of Escherichia coli agonists sensed by innate immunity receptors of the bovine udder and mammary epithelial cells. Veterinary Research 43: 14.
'Repertoire of Escherichia coli agonists sensed by innate immunity receptors of the bovine udder and mammary epithelial cells ' () 43 Veterinary Research : 14.
-
R development Core Team, 2013. R: a language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. Available at: http://www.R-project.org.
-
Rainard, P. and Riollet, C., 2006. Innate immunity of the bovine mammary gland. Veterinary Research 37: 369-400.
'Innate immunity of the bovine mammary gland ' () 37 Veterinary Research : 369 -400.
-
Rochat, T., Bermúdez-Humarán, L., Gratadoux, J.-J., Fourage, C., Hoebler, C., Corthier, G. and Langella, P., 2007. Anti-inflammatory effects of Lactobacillus casei BL23 producing or not a manganese-dependant catalase on DSS-induced colitis in mice. Microbial Cell Factories 6: 22.
'Anti-inflammatory effects of Lactobacillus casei BL23 producing or not a manganese-dependant catalase on DSS-induced colitis in mice ' () 6 Microbial Cell Factories : 22.
-
Roussel, P., Cunha, P., Porcherie, A., Petzl, W., Gilbert, F.B., Riollet, C., Zerbe, H., Rainard, P. and Germon, P., 2015. Investigating the contribution of IL-17A and IL-17F to the host response during Escherichia coli mastitis. Veterinary Research 46: 56.
'Investigating the contribution of IL-17A and IL-17F to the host response during Escherichia coli mastitis ' () 46 Veterinary Research : 56.
-
Sánchez, B., Delgado, S., Blanco-Míguez, A., Lourenço, A., Gueimonde, M. and Margolles, A., 2017. Probiotics, gut microbiota, and their influence on host health and disease. Molecular Nutrition and Food Research 61: 1600240.
'Probiotics, gut microbiota, and their influence on host health and disease ' () 61 Molecular Nutrition and Food Research : 1600240.
-
Schröder, N.W.J., Morath, S., Alexander, C., Hamann, L., Hartung, T., Zähringer, U., Göbel, U.B., Weber, J.R. and Schumann, R.R., 2003. Lipoteichoic acid (LTA) of Streptococcus pneumoniae and Staphylococcus aureus activates immune cells via toll-like receptor (TLR)-2, lipopolysaccharide-binding protein (LBP), and CD14, whereas TLR-4 and MD-2 are not involved. Journal of Biological Chemistry 278: 15587-15594.
'Lipoteichoic acid (LTA) of Streptococcus pneumoniae and Staphylococcus aureus activates immune cells via toll-like receptor (TLR)-2, lipopolysaccharide-binding protein (LBP), and CD14, whereas TLR-4 and MD-2 are not involved ' () 278 Journal of Biological Chemistry : 15587 -15594.
-
Shuster, D.E., Kehrli, M.E., Rainard, P. and Paape, M., 1997. Complement fragment C5a and inflammatory cytokines in neutrophil recruitment during intramammary infection with Escherichia coli. Infection and Immunity 65: 3286-3292.
'Complement fragment C5a and inflammatory cytokines in neutrophil recruitment during intramammary infection with Escherichia coli ' () 65 Infection and Immunity : 3286 -3292.
-
Sprague, A.H. and Khalil, R.A., 2009. Inflammatory cytokines in vascular dysfunction and vascular disease. Biochemical Pharmacology 78: 539-552.
'Inflammatory cytokines in vascular dysfunction and vascular disease ' () 78 Biochemical Pharmacology : 539 -552.
-
Takeuchi, O., Hoshino, K. and Akira, S., 2000. Cutting edge: TLR2-deficient and MyD88-deficient mice are highly susceptible to Staphylococcus aureus infection. Journal of Immunology 165: 5392-5396.
'Cutting edge: TLR2-deficient and MyD88-deficient mice are highly susceptible to Staphylococcus aureus infection ' () 165 Journal of Immunology : 5392 -5396.
-
Wang, L., Si, W., Xue, H. and Zhao, X., 2017. A fibronectin-binding protein (FbpA) of Weissella cibaria inhibits colonization and infection of Staphylococcus aureus in mammary glands. Cellular Microbiology 19: e12731.
'A fibronectin-binding protein (FbpA) of Weissella cibaria inhibits colonization and infection of Staphylococcus aureus in mammary glands ' () 19 Cellular Microbiology : e12731.
-
Zheng, H., Fletcher, D., Kozak, W., Jiang, M., Hofmann, K.J., Conn, C.A., Soszynski, D., Grabiec, C., Trumbauer, M.E. and Shaw, A., 1995. Resistance to fever induction and impaired acute-phase response in interleukin-1 beta-deficient mice. Immunity 3: 9-19.
'Resistance to fever induction and impaired acute-phase response in interleukin-1 beta-deficient mice ' () 3 Immunity : 9 -19.
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Lactobacillus casei BL23 modulates the innate immune response in Staphylococcus aureus-stimulated bovine mammary epithelial cells
In: Beneficial MicrobesAgrocampus Ouest, UMR 1253 STLO, 85 Rue de Saint Brieuc, 35042 Rennes, France.
Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, 31270-901 MG, Brazil.
Universidade Federal do Vale do São Francisco, Av. José de Sá Maniçoba, S/N Centro, Petrolina, 56304-917 PE, Brazil.
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Agrocampus Ouest, UMR 1253 STLO, 85 Rue de Saint Brieuc, 35042 Rennes, France.
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Agrocampus Ouest, UMR 1253 STLO, 85 Rue de Saint Brieuc, 35042 Rennes, France.
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Agrocampus Ouest, UMR 1253 STLO, 85 Rue de Saint Brieuc, 35042 Rennes, France.
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Probiotics have been adopted to treat and prevent various diseases in humans and animals. They were notably shown to be a promising alternative to prevent mastitis in dairy cattle. This inflammation of the mammary gland is generally of infectious origin and generates extensive economic losses worldwide. In a previous study, we found that Lactobacillus casei BL23 was able to inhibit the internalisation of Staphylococcus aureus, one of the major pathogens involved in mastitis, into bovine mammary epithelial cells (bMEC). In this study, we further explored the capacity of this strain to modulate the innate immune response of bovine mammary epithelial cells during S. aureus infection. L. casei BL23 was able to decrease the expression of several pro-inflammatory cytokines, including interleukins 6, 8, 1α and 1β and tumour necrosis factor alpha, in S. aureus-stimulated bMEC, 8 h post-infection. On the other hand, L. casei did not impair the induction of defensins, such as lingual antimicrobial peptide and defensin β1 in the presence of S. aureus, and even slightly increased the induction of tracheal antimicrobial peptide during S. aureus infection. Finally, this strain did not alter the expression of the pattern recognition receptor nucleotide-binding oligomerisation domain proteins (NOD2). This study demonstrates that L. casei BL23 displayed anti-inflammatory properties on S. aureus-stimulated bMEC. These results open the way to further characterisation of the BL23 probiotic potential in a bovine mammary gland context and to a better understanding of how all these beneficial properties combine in vivo to combat mastitis pathogens.
| All Time | Past 365 days | Past 30 Days | |
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| Abstract Views | 692 | 244 | 15 |
| Full Text Views | 10 | 4 | 2 |
| PDF Views & Downloads | 12 | 4 | 1 |
