In our previous study we confirmed an antiviral activity of probiotic Lactobacillus reuteri L26 which was mediated by stimulation of local intestinal immunity. The aim of this paper was to evaluate the influence of L. reuteri L26 on the systemic immune response in gnotobiotic mice infected with porcine circovirus type 2 (PCV2). A total of 30 germ-free mice were divided into 3 groups and animals in noninfected and infected control groups (NC and IC; n=10) received sterile de Man-Rogosa-Sharpe broth for 7 days and animals in experimental group L+PCV (n=10) were inoculated with L. reuteri L26. Subsequently, mice in L+PCV and IC groups were infected with PCV2; however, mice in the control group received virus cultivation medium (mock). The results showed an increase of percentage of cytotoxic cells (CD8+ and CD49b+CD8-) and oxidative burst of phagocytes, up-regulation of the gene expression of RANTES, granulocyte-macrophage colony-stimulating factor, interferon-γ and immunoglobulin A in blood above all in the later phase of infection (14 dpi) in L+PCV group accompanied by higher load of PCV2 in the serum. These findings indicate that L. reuteri L26 has a potential to induce systemic immune reaction, but in gnotobiotic mice immune stimulation can increase virus replication.
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Al Kassaa, I., Hober, D., Hamze, M., Chihib, N.E. and Drider, D., 2014. Antiviral potential of lactic acid bacteria and their bacteriocins. Probiotics and Antimicrobial Proteins 6: 177-185.
'Antiviral potential of lactic acid bacteria and their bacteriocins ' () 6 Probiotics and Antimicrobial Proteins : 177 -185.
Allan, G.M. and Ellis, J.A., 2000. Porcine circoviruses: a review. Journal of Veterinary Diagnostic Investigation 12: 3-14.
'Porcine circoviruses: a review ' () 12 Journal of Veterinary Diagnostic Investigation : 3 -14.
Allan, G.M., Kennedy, S., McNeilly, F., Foster, J.C., Ellis, J.A., Krakowka, S.J, Meehan, B.M. and Adair, B.M., 1999. Experimental reproduction of sever wasting disease by coinfection of pigs with porcine circovirus and porcine parvovirus. Journal of Comparative Pathology 121: 1-11.
'Experimental reproduction of sever wasting disease by coinfection of pigs with porcine circovirus and porcine parvovirus ' () 121 Journal of Comparative Pathology : 1 -11.
Aravindaram, K., Kuo, T.Y., Lan, C.W., Yu, H.H., Wang, P.H., Chen, Y.S., Chen, G.H. and Yang, N.S., 2009. Protective immunity against porcine circovirus 2 in mice induced by a gene-based combination vaccination. Journal of Gene Medicine 11: 288-301.
'Protective immunity against porcine circovirus 2 in mice induced by a gene-based combination vaccination ' () 11 Journal of Gene Medicine : 288 -301.
Borchers, A.T., Selmi, C., Meyers, F.J., Keen, C.L. and Gershwin, M.E., 2009. Probiotics and immunity. Journal of Gastroenterology 44: 26-46.
'Probiotics and immunity ' () 44 Journal of Gastroenterology : 26 -46.
Chattha, K.S., Vlasova, A.N., Kandasamy, S., Rajashekara, G. and Saif, L.J., 2013. Divergent immunomodulating effects of probiotics on t cell responses to oral attenuated human rotavirus vaccine and virulent human rotavirus infection in a neonatal gnotobiotic piglet disease model. Journal of Immunology 191: 2446-2456.
'Divergent immunomodulating effects of probiotics on t cell responses to oral attenuated human rotavirus vaccine and virulent human rotavirus infection in a neonatal gnotobiotic piglet disease model ' () 191 Journal of Immunology : 2446 -2456.
Choi, H.J., Song, J.H., Park, K.S., Baek, S.H., Lee, E.S. and Kwon, D.H., 2010. Antiviral activity of yogurt against enterovirus 71 in vero cells. Food Science and Biotechnology 19: 289-295.
'Antiviral activity of yogurt against enterovirus 71 in vero cells ' () 19 Food Science and Biotechnology : 289 -295.
Crouse, J., Xu, H.C., Lang, P.A. and Oxenius, A., 2015. NK cells regulating T cell responses: mechanisms and outcome. Trends in Immunology 36: 49-58.
'NK cells regulating T cell responses: mechanisms and outcome ' () 36 Trends in Immunology : 49 -58.
Cságola, A., Cadar, D. and Tuboly, T., 2008. Replication and transmission of porcine circovirus type 2 in experimentally inoculated mice. Acta Veterinaria Hungarica 55: 421-427.
'Replication and transmission of porcine circovirus type 2 in experimentally inoculated mice ' () 55 Acta Veterinaria Hungarica : 421 -427.
Csank, T., Pistl, J., Polláková, J., Bhide, K., Herich, R. and Levkut, M., 2013. Dynamics of antibody response and viraemia following natural infection of porcine circovirus 2 (PCV-2) in a conventional pig herd. Acta Pathologica, Microbiologica et Imnuologica Scandinavica 121: 1207-1213.
'Dynamics of antibody response and viraemia following natural infection of porcine circovirus 2 (PCV-2) in a conventional pig herd ' () 121 Acta Pathologica, Microbiologica et Imnuologica Scandinavica : 1207 -1213.
Darwich, L. and Mateu, E., 2012. Immunology of porcine circovirus type 2 (PCV2). Virus Research 164: 61-67.
'Immunology of porcine circovirus type 2 (PCV2) ' () 164 Virus Research : 61 -67.
Darwich, L., Segales, J. and Mateu, E., 2004. Pathogenesis of postweaning multisystemic wasting syndrome caused by porcine circovirus 2: an immune riddle. Archives of Virology 149: 857-874.
'Pathogenesis of postweaning multisystemic wasting syndrome caused by porcine circovirus 2: an immune riddle ' () 149 Archives of Virology : 857 -874.
Darwich, L., Segalés, J., Domingo, M. and Mateu, E., 2002. Changes in CD4+, CD8+, CD4+ CD8+, and immunoglobulin M-positive peripheral blood mononuclear cells of postweaning multisystemic wasting syndrome-affected pigs and age-matched uninfected wasted and health pigs correlate with lesions and porcine circovirus type 2 load in lymphoid tissues. Clinical and Diagnostic Laboratory Immunology 9: 236-242.
'Changes in CD4+, CD8+, CD4+ CD8+, and immunoglobulin M-positive peripheral blood mononuclear cells of postweaning multisystemic wasting syndrome-affected pigs and age-matched uninfected wasted and health pigs correlate with lesions and porcine circovirus type 2 load in lymphoid tissues ' () 9 Clinical and Diagnostic Laboratory Immunology : 236 -242.
Dong, H., Rowland, I., Tuohy, K.M., Thomas, L.V. and Yaqoob, P., 2010. Selective effects of Lactobacillus casei Shirota on T cell activation, natural killer cell activity and cytokine production. Clinical and Experimental Immunology 161: 378-388.
'Selective effects of Lactobacillus casei Shirota on T cell activation, natural killer cell activity and cytokine production ' () 161 Clinical and Experimental Immunology : 378 -388.
Fenaux, M., Opriessnig, T., Halbur, P.G., Elvinger, F. and Meng, X.J., 2004. A chimeric porcine circovirus (PCV) with the immunogenic capsid gene of the pathogenic PCV type 2 (PCV2) cloned into the genomic backbone of the nonpathogenic PCV1 induces protective immunity against PCV2 infection in pigs. Journal of Virology 78: 6297-6303.
'A chimeric porcine circovirus (PCV) with the immunogenic capsid gene of the pathogenic PCV type 2 (PCV2) cloned into the genomic backbone of the nonpathogenic PCV1 induces protective immunity against PCV2 infection in pigs ' () 78 Journal of Virology : 6297 -6303.
Food and Agriculture Organization of the United Nations/World Health Organization (FAO/WHO), 2002. Guidelines for the evaluation of probiotics in food. Report of a Joint FAO/WHO working group on drafting guidelines for the evaluation of probiotics in food. Available at: http://tinyurl.com/zdbrkeg.
GancarÄÃková, S., Popper, M., HrÄková, G., MaÄar, M., MudroÅová, D., Sopková, D. and Nemcová, R., 2018. Antibiotic-treated SPF mice as a gnotobiotic model. In: Savic, S. (ed.) Antibiotic use in animals. InTech, Rijeka, Croatia, pp. 39.
'Antibiotic-treated SPF mice as a gnotobiotic model ', in Antibiotic use in animals , () 39.
GancarÄÃková, S., Sciranková, Ľ., Popper, M., MaÄar, M., Nemcová, R., MudroÅová, D., KoÅ¡Äová, J. and ŽitÅan, R., 2016. Influence of a probiotic strain stabilized on fossil carrier on intestinal microenvironment of conventional SPF mice [Slovak]. In: Proceedings of the XII International Scientific Conference: Lazar´s Days of Nutrition and Veterinary Dietetics. September 7-8, 2016, pp. 259-263.
'Influence of a probiotic strain stabilized on fossil carrier on intestinal microenvironment of conventional SPF mice [Slovak] ', in Proceedings of the XII International Scientific Conference: Lazar´s Days of Nutrition and Veterinary Dietetics , () 259 -263.
Gärdby, E. and Lycke, N.Y., 2000. CD19-deficient mice exhibit poor responsiveness to oral immunization despite evidence of unaltered total IgA levels, germinal centers and IgA-isotype switching in Peyerâs patches. European Journal of Immunology 30: 1861-1871.
'CD19-deficient mice exhibit poor responsiveness to oral immunization despite evidence of unaltered total IgA levels, germinal centers and IgA-isotype switching in Peyerâs patches ' () 30 European Journal of Immunology : 1861 -1871.
Gilpin, D.F., McCullough, K., Meehan, B.M., McNeilly, F., McNair, I., Stevenson, L.S., Foster, J.C., Ellis, J.A., Krakowka, S., Adair, B.M. and Allan, G.M., 2003. In vitro studies on the infection and replication of porcine circovirus type 2 in cells of the porcine immune system. Veterinary Immunology and Immunopathology 94: 149-161.
'In vitro studies on the infection and replication of porcine circovirus type 2 in cells of the porcine immune system ' () 94 Veterinary Immunology and Immunopathology : 149 -161.
Grégoire, C., Chasson, L., Luci, C., Tomasello, E., Geissmann, F., Vivier, E. and Walzer, T., 2007. The trafficking of natural killer cells. Immunological Reviews 220: 169-182.
'The trafficking of natural killer cells ' () 220 Immunological Reviews : 169 -182.
Guarino, A., Lo Vecchio, A. and Canani, R.B., 2009. Probiotics as prevention and treatment for diarrhea. Current Opinion in Gastroenterology 25: 18-23.
'Probiotics as prevention and treatment for diarrhea ' () 25 Current Opinion in Gastroenterology : 18 -23.
Jensen, T.K., Vigre, H., Svensmark, B. and Bille-Hansen, V., 2006. Distinction between porcine Circovirus type 2 enteritis and porcine proliferative enteropathy caused by Lawsonia intracellularis. Journal of Comparative Pathology 136: 176-182.
'Distinction between porcine Circovirus type 2 enteritis and porcine proliferative enteropathy caused by Lawsonia intracellularis ' () 136 Journal of Comparative Pathology : 176 -182.
Karaffová, V., Csank, T., MudroÅová, D., Király, J., Revajová, V., GancarÄÃková, S., Nemcová, R., Pistl, J., VilÄek, Å . and Levkut, M., 2017. The influence of Lactobacillus reuteri L26 Biocenol⢠on immune response against porcine circovirus type 2 infection in germ-free mice. Beneficial Microbes 8: 367-378.
'The influence of Lactobacillus reuteri L26 Biocenol⢠on immune response against porcine circovirus type 2 infection in germ-free mice ' () 8 Beneficial Microbes : 367 -378.
Kim, D.H., Sandova, D., Reed, J.A., Matter, E.K., Tolod, E.G. Woods, S.C. and Seeley, R.J., 2008. The role of GM-CSF in adipose tissue inflammation. American Journal of Physiology â Endocrinology and Metabolism 295: E1038-E1046.
'The role of GM-CSF in adipose tissue inflammation ' () 295 American Journal of Physiology â Endocrinology and Metabolism : E1038 -E1046.
Knight, P.A., Wright, S.H., Brown, J.K., Huang, X., Sheppard, D. and Miller, H.R.P., 2002. Enteric expression of the integrin αvβ6 is essential for nematode-induced mucosal mast cell hyperplasia and expression of the granule chymase, mouse mast cell protease-1. American Journal of Pathology 161: 771-779.
'Enteric expression of the integrin αvβ6 is essential for nematode-induced mucosal mast cell hyperplasia and expression of the granule chymase, mouse mast cell protease-1 ' () 161 American Journal of Pathology : 771 -779.
Kozakova, H., Schwarzer, M., Tuckova, L., Srutkova, D., Czarnowska, E., Rosiak, I., Hudcovic, T., Schabussova, I., Hermanova, P., Zakostelska, Z., Aleksandrzak-Piekarczyk, T., Koryszewska-Baginska, A., Tlaskalova-Hogenova, H. and Cukrowska, B., 2016. Colonization of germ-free mice with a mixture of three Lactobacillus strains enhances the integrity of gut mucosa and ameliorates allergic sensitization. Cellular & Molecular Immunology 13: 251-262.
'Colonization of germ-free mice with a mixture of three Lactobacillus strains enhances the integrity of gut mucosa and ameliorates allergic sensitization ' () 13 Cellular & Molecular Immunology : 251 -262.
KÅ¡onžeková, P., Bystrický, P., VlÄková, S., Pätoprstý, V., Pulzová, L., MudroÅová, D., KubaÅ¡ková, T., Csank, T. and TkáÄiková, Ľ., 2016. Exopolysaccharides of Lactobacillus reuteri: their influence on adherence of E. coli to epithelial cells and inflammatory response. Carbohydrate Polymers 141: 10-19.
'Exopolysaccharides of Lactobacillus reuteri: their influence on adherence of E. coli to epithelial cells and inflammatory response ' () 141 Carbohydrate Polymers : 10 -19.
Ladekjær-Mikkelsen, A.S., Nielsen, J., Stadejek, T., Storgaard, T., Krakowka, S., Ellis, J., McNeilly, F., Allan, G. and Bøtner, A., 2002. Reproduction of postweaning multisystemic wasting syndrome (PMWS) in immunostimulated and non-immunostimulated 3-week-old piglets experimentally infected with porcine circovirus type 2 (PCV2). Veterinary Microbiology 89: 97-114.
'Reproduction of postweaning multisystemic wasting syndrome (PMWS) in immunostimulated and non-immunostimulated 3-week-old piglets experimentally infected with porcine circovirus type 2 (PCV2) ' () 89 Veterinary Microbiology : 97 -114.
Lefebvre, D.J., Costers, S., Van Doorsselaere, J., Misinzo, G., Delputte, P.L. and Nauwynck, H.J., 2008. Antigenic differences among porcine circovirus type 2 strains, as demonstrated by the use of monoclonal antibodies. Journal of General Virology 89: 177-187.
'Antigenic differences among porcine circovirus type 2 strains, as demonstrated by the use of monoclonal antibodies ' () 89 Journal of General Virology : 177 -187.
Lemme-Dumit, J.M., Polti, M.A., Perdigón, G. and Galdeano, C.M., 2018. Probiotic bacteria cell walls stimulate the activity of the intestinal epithelial cells and macrophage functionality. Beneficial Microbes 9: 153-164.
'Probiotic bacteria cell walls stimulate the activity of the intestinal epithelial cells and macrophage functionality ' () 9 Beneficial Microbes : 153 -164.
Liu, J., Chen, I., Du, Q., Chua, H. and Kwang, J., 2006. The ORF3 protein of porcine circovirus type 2 is involved in viral pathogenesis in vivo. Journal of Virology 80: 5065-5073.
'The ORF3 protein of porcine circovirus type 2 is involved in viral pathogenesis in vivo ' () 80 Journal of Virology : 5065 -5073.
Liu, W.H., Chuang, H.L., Huang, Y.T., Wu, C.C., Chou, G.T., Wang, S. and Tsaia, Y.C., 2016. Alteration of behavior and monoamine levels attributable to Lactobacillus plantarum PS128 in germ-free mice. Behavioural Brain Research 298: 202-209.
'Alteration of behavior and monoamine levels attributable to Lactobacillus plantarum PS128 in germ-free mice ' () 298 Behavioural Brain Research : 202 -209.
Lorincz, M., Cságola, A., Biksi, I., Szeredi, L., Dán, A. and Tuboly, T., 2010. Detection of porcine circovirus in rodents â short communication. Acta Veterinaria Hungarica 58: 265-268.
'Detection of porcine circovirus in rodents â short communication ' () 58 Acta Veterinaria Hungarica : 265 -268.
Macpherson, A.J., Gatto, D., Sainsbury, E., Harriman, G.R., Hengartner, H. and Zinkernagel, R.M., 2000. A primitive T cell-independent mechanism of intestinal mucosal IgA responses to commensal bacteria. Science 288: 2222-2226.
'A primitive T cell-independent mechanism of intestinal mucosal IgA responses to commensal bacteria ' () 288 Science : 2222 -2226.
Mastromarino, P., Cacciotti, F., Masci, A. and Mosca, L., 2011. Antiviral activity of Lactobacillus brevis towards herpes simplex virus type 2: role of cell wall associated components. Anaerobe 17: 334-336.
'Antiviral activity of Lactobacillus brevis towards herpes simplex virus type 2: role of cell wall associated components ' () 17 Anaerobe : 334 -336.
Matsusaki, T., Takeda, S., Takeshita, M., Arima, Y., Tsend-Ayush, C., Oyunsuren, T., Sugita, C., Yoshida, H., Watanabe, W. and Kurokawa, M., 2016. Augmentation of T helper type 1 immune response through intestinal immunity in murine cutaneous herpes simplex virus type 1 infection by probiotic Lactobacillus plantarum strain 06CC2. International Immunopharmacology 39: 320-327.
'Augmentation of T helper type 1 immune response through intestinal immunity in murine cutaneous herpes simplex virus type 1 infection by probiotic Lactobacillus plantarum strain 06CC2 ' () 39 International Immunopharmacology : 320 -327.
Meerts, P., Misinzo, G., McNeilly, F. and Nauwynck, H.J., 2005. Replication kinetics of different porcine circovirus 2 strains in PK-15 cells, fetal cardiomyocytes and macrophages. Archives of Virology 150: 427-441.
'Replication kinetics of different porcine circovirus 2 strains in PK-15 cells, fetal cardiomyocytes and macrophages ' () 150 Archives of Virology : 427 -441.
Moloney, R.D., Desbonnet, L., Clarke, G., Dinan, T.G. and Cryan, J.F., 2014. The microbiome: stress, health and disease. Mammalian Genome 25: 49-74.
'The microbiome: stress, health and disease ' () 25 Mammalian Genome : 49 -74.
Nagpal, R., Kumar, M., Yadav, A.K., Hemalatha, R., Yadav, H., Marotta, F. and Yamashiro, Y., 2016. Gut microbiota in health and disease: an overview focused on metabolic inflammation. Beneficial Microbes 7: 181-194.
'Gut microbiota in health and disease: an overview focused on metabolic inflammation ' () 7 Beneficial Microbes : 181 -194.
Nielsen, J., Vincent, I.E., Bøtner, A., Ladekjaer-Mikkelsen, A.S., Allan, G., Summerfield, A. and McCullough, K.C., 2003. Association of lymphopenia with porcine circovirus type 2 induced postweaning multisystemic wasting syndrome (PMWS). Veterinary Immunology and Immunopathology 92: 97-111.
'Association of lymphopenia with porcine circovirus type 2 induced postweaning multisystemic wasting syndrome (PMWS) ' () 92 Veterinary Immunology and Immunopathology : 97 -111.
Reid, G., Jass, J., Sebulsky, M.T. and McCormick, J.K., 2003. Potential uses of probiotics in clinical practice. Clinical Microbiology Reviews 16: 658-672.
'Potential uses of probiotics in clinical practice ' () 16 Clinical Microbiology Reviews : 658 -672.
Rosell, C., Segalés, J., Plana-Durán, J., Balasch, M., RodrÃguez-Arrioja, G.M., Kennedy, S., Allan, G.M., McNeilly, F., Latimer, K.S. and Domingo, M., 1999. Pathological, immunohistochemical, and in-situ hybridization studies of natural cases of postweaning multisystemic wasting syndrome (PMWS) in pigs. Journal of Comparative Pathology 120: 59-78.
'Pathological, immunohistochemical, and in-situ hybridization studies of natural cases of postweaning multisystemic wasting syndrome (PMWS) in pigs ' () 120 Journal of Comparative Pathology : 59 -78.
Ryznerová, D., 2013. The study of the properties of the probiotic bacteria in terms of their biological effects and applications. PhD-thesis, University of Veterinary Medicine in Kosice, Košice, Slovak Republic, 148 pp.
'The study of the properties of the probiotic bacteria in terms of their biological effects and applications ', () 148.
Segalés, J., 2015. Best practice and future challenges for vaccination against porcine circovirus type 2. Expert Review of Vaccines 14: 473-487.
'Best practice and future challenges for vaccination against porcine circovirus type 2 ' () 14 Expert Review of Vaccines : 473 -487.
Segalés, J., Alonso, F., Rosell, C., Pastor, J., Chianini, F., Campos, E., López-Fuertes, L., Quintana, J., RodrÃguez-Arrioja, G., Calsamiglia, M., Pujols, J., DomÃnguez, J. and Domingo, M., 2001. Changes in peripheral blood leukocyte populations in pigs with natural postweaning multisystemic wasting syndrome (PMWS). Veterinay Immunology and Immunopathology 81: 37-44.
'Changes in peripheral blood leukocyte populations in pigs with natural postweaning multisystemic wasting syndrome (PMWS) ' () 81 Veterinay Immunology and Immunopathology : 37 -44.
Simon Davis, D.A. and Parish, C.R., 2013. Heparan sulfate: a ubiquitous glycosaminoglycan with multiple roles in immunity. Frontiers in Immunology 4: 1-7.
'Heparan sulfate: a ubiquitous glycosaminoglycan with multiple roles in immunity ' () 4 Frontiers in Immunology : 1 -7.
Sisto, F., Miluzio, A., Leopardi, O., Mirra, M., Boelaert, J.R. and Taramelli, D., 2003. Differential cytokine pattern in the spleens and livers of BALB/c mice infected with Penicillium marneffei: protective role of gamma interferon. Infection and Immunity 71: 465-473.
'Differential cytokine pattern in the spleens and livers of BALB/c mice infected with Penicillium marneffei: protective role of gamma interferon ' () 71 Infection and Immunity : 465 -473.
Truusalu, K., Kullisaar, T., Hütt, P., Mahlapuu, R., Aunapuu, M., Arend, A., Zilmer, M., Mikelsaar, R.H. and Mikelsaar, M., 2010. Immunological, antioxidative, and morphological response in combined treatment of ofloxacin and Lactobacillus fermentum ME-3 probiotic in Salmonella Typhimurium murine model. Acta Pathologica, Microbiologica et Imnuologica Scandinavica 118: 864-872.
'Immunological, antioxidative, and morphological response in combined treatment of ofloxacin and Lactobacillus fermentum ME-3 probiotic in Salmonella Typhimurium murine model ' () 118 Acta Pathologica, Microbiologica et Imnuologica Scandinavica : 864 -872.
Vandenplas, Y., 2015. Healthy gut microbiota and long term health. Beneficial Microbes 6: 173-179.
'Healthy gut microbiota and long term health ' () 6 Beneficial Microbes : 173 -179.
Von Hundelshausen, P., Weber, K.S., Huo, Y., Proudfoot, A.E., Nelson, P.J., Ley, K. and Weber, C., 2001. RANTES deposition by platelets triggers monocyte arrest on inflamed and atherosclerotic endothelium. Circulation 103: 1772-1777.
'RANTES deposition by platelets triggers monocyte arrest on inflamed and atherosclerotic endothelium ' () 103 Circulation : 1772 -1777.
Walzer, T., Chiossone, L., Chaix, J., Calver, A., Carozzo, C., Garrigue-Antar, L., Jacques, Y., Baratin, M., Tomasello, E. and Vivier, E., 2007. Natural killer cell trafficking in vivo requires a dedicated sphingosine 1-phosphate receptor. Nature Immunology 8: 1337-1344.
'Natural killer cell trafficking in vivo requires a dedicated sphingosine 1-phosphate receptor ' () 8 Nature Immunology : 1337 -1344.
Wellenberg, G.J., Pesh, S., Berndsen, F.W., Steverink, P.J., Hunneman, W., Van der Vorst, T.J., Peperkamp, N.H., Ohlinger, V.F., Schippers, R., Van Oirschot, J.T. and De Jong, M.F., 2000. Isolation and characterization of porcine circovirus type 2 from pigs showing signs of post-weaning multisystemic wasting syndrome in the Netherlands. Veterinary Quarterly 22: 167-172.
'Isolation and characterization of porcine circovirus type 2 from pigs showing signs of post-weaning multisystemic wasting syndrome in the Netherlands ' () 22 Veterinary Quarterly : 167 -172.
Xu, X.G., Zhao, H.N., Zhang, Q., Ding, L., Li, Z.L., Li, W., Wu, H.Y., Chuang, K.P., Tong, D.W. and Liu, H.J., 2012. Oral vaccination with attenuated Salmonella enterica serovar Typhimurium expressing Cap protein of PCV2 and its immunogenicity in mouse and swine models. Veterinary Microbiology 157: 294-303.
'Oral vaccination with attenuated Salmonella enterica serovar Typhimurium expressing Cap protein of PCV2 and its immunogenicity in mouse and swine models ' () 157 Veterinary Microbiology : 294 -303.
| All Time | Past 365 days | Past 30 Days | |
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In our previous study we confirmed an antiviral activity of probiotic Lactobacillus reuteri L26 which was mediated by stimulation of local intestinal immunity. The aim of this paper was to evaluate the influence of L. reuteri L26 on the systemic immune response in gnotobiotic mice infected with porcine circovirus type 2 (PCV2). A total of 30 germ-free mice were divided into 3 groups and animals in noninfected and infected control groups (NC and IC; n=10) received sterile de Man-Rogosa-Sharpe broth for 7 days and animals in experimental group L+PCV (n=10) were inoculated with L. reuteri L26. Subsequently, mice in L+PCV and IC groups were infected with PCV2; however, mice in the control group received virus cultivation medium (mock). The results showed an increase of percentage of cytotoxic cells (CD8+ and CD49b+CD8-) and oxidative burst of phagocytes, up-regulation of the gene expression of RANTES, granulocyte-macrophage colony-stimulating factor, interferon-γ and immunoglobulin A in blood above all in the later phase of infection (14 dpi) in L+PCV group accompanied by higher load of PCV2 in the serum. These findings indicate that L. reuteri L26 has a potential to induce systemic immune reaction, but in gnotobiotic mice immune stimulation can increase virus replication.
| All Time | Past 365 days | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 366 | 114 | 24 |
| Full Text Views | 14 | 0 | 0 |
| PDF Views & Downloads | 9 | 0 | 0 |