Extraction of the same novel homoglycan mixture from two different strains of Bifidobacterium animalis and three strains of Bifidobacterium breve
äºBeneficial MicrobesSearch for other papers by M. Alhudhud in
Current site
Google Scholar
Search for other papers by S. Sadiq in
Current site
Google Scholar
Search for other papers by H.N. Ngo in
Current site
Google Scholar
Search for other papers by C. Hidalgo-Cantabrana in
Current site
Google Scholar
Search for other papers by P. Ruas-Madiedo in
Current site
Google Scholar
Search for other papers by D. van Sinderen in
Current site
Google Scholar
Search for other papers by P.N. Humphreys in
Current site
Google Scholar
Search for other papers by A.P. Laws in
Current site
Google Scholar
Three strains of Bifidobacterium breve (JCM 7017, JCM 7019 and JCM 2258) and two strains of Bifidobacterium animalis subsp. lactis (AD011 and A1dOxR) were grown in broth cultures or on plates, and a standard exopolysaccharide extraction method was used in an attempt to recover exocellular polysaccharides. When the extracted materials were analysed by NMR it was clear that mixtures of polysaccharides were being isolated including exopolysaccharides (EPS) cell wall polysaccharides and intracellular polysaccharides. Treatment of the cell biomass from the B. breve strains, or the B. animalis subsp. lactis AD011 strain, with aqueous sodium hydroxide provided a very similar mixture of polysaccharides but without the EPS. The different polysaccharides were partially fractionated by selective precipitation from an aqueous solution upon the addition of increasing percentages of ethanol. The polysaccharides extracted from B. breve JCM 7017 grown in HBM media supplemented with glucose (or isotopically labelled D-glucose-1-13C) were characterised using 1D and 2D-NMR spectroscopy. Addition of one volume of ethanol generated a medium molecular weight glycogen (Mw=1Ã105 Da, yield 200 mg/l). The addition of two volumes of ethanol precipitated an intimate mixture of a low molecular weight β-(1â6)-glucan and a low molecular weight β-(1â6)-galactofuranan which could not be separated (combined yield 46 mg/l). When labelled D-glucose-1-13C was used as a carbon supplement, the label was incorporated into >95% of the anomeric carbons of each polysaccharide confirming they were being synthesised in situ. Similar 1H NMR profiles were obtained for polysaccharides recovered from the cells of B. animalis subsp. lactis AD011and A1dOxR (in combination with an EPS), B. breve JCM 7017, B. breve JCM 7019, B. breve JCM 2258 and from an EPS (-ve) mutant of B. breve 7017 (a non-EPS producer).
Purchase
Buy instant access (PDF download and unlimited online access):
Institutional Login
Log in with Open Athens, Shibboleth, or your institutional credentials
Personal login
Log in with your brill.com account
-
Alhudhud, M., Humphreys, P. and Laws, A., 2014. Development of a growth medium suitable for exopolysaccharide production and structural characterisation by Bifidobacterium animalis ssp. lactis AD011. Journal of Microbiological Methods 100: 93-98.
'Development of a growth medium suitable for exopolysaccharide production and structural characterisation by Bifidobacterium animalis ssp ' () 100 Journal of Microbiological Methods : 93 -98.
-
Altmann, F., Kosma, P., OâCallaghan, A., Leahy, S., Bottacini, F., Molloy, E., Plattner, S., Schiavi, E., Gleinser, M., Groeger, D., Grant, R., Perez, N.R., Healy, S., Svehla, E., Windwarder, M., Hofinger, A., Motherway, M.O., Akdis, C.A., Xu, J., Roper, J., Van Sinderen, D. and OâMahony, L., 2016. Genome analysis and characterisation of the exopolysaccharide produced by Bifidobacterium longum subsp. longum 35624â¢. PLoS ONE 11: e0162983.
'Genome analysis and characterisation of the exopolysaccharide produced by Bifidobacterium longum subsp ' () 11 PLoS ONE : e0162983.
-
Arboleya, S., Watkins, C., Stanton, C. and Ross, R.P., 2016. Gut bifidobacteria populations in human health and aging. Frontiers in Microbiology 7: 1204.
'Gut bifidobacteria populations in human health and aging ' () 7 Frontiers in Microbiology : 1204.
-
Balzaretti, S., Taverniti, V., Guglielmetti, S., Fiore, W., Minuzzo, M., Ngo, H.N., Ngere, J.B., Sadiq, S., Humphreys, P.N. and Laws, A.P., 2017. A novel rhamnose-rich hetero-exopolysaccharide isolated from Lactobacillus paracasei DG activates THP-1 human monocytic cells. Applied and Environmental Microbiology 83: e02702-16.
'A novel rhamnose-rich hetero-exopolysaccharide isolated from Lactobacillus paracasei DG activates THP-1 human monocytic cells ' () 83 Applied and Environmental Microbiology : e02702 -16.
-
Bertin, C., Pau-Roblot, C., Courtois, J., Manso-Silván, L., Thiaucourt, F., Tardy, F., Le Grand, D., Poumarat, F. and Gaurivaud, P., 2013. Characterization of free exopolysaccharides secreted by Mycoplasma mycoides subsp. mycoides. PLoS ONE 8: e68373.
'Characterization of free exopolysaccharides secreted by Mycoplasma mycoides subsp ' () 8 PLoS ONE : e68373.
-
Bock, K. and Pedersen, C., 1974. A study of 13CH coupling constants in hexopyranoses. Journal of the Chemical Society, Perkin Transactions 2: 293-297.
'A study of 13CH coupling constants in hexopyranoses ' () 2 Journal of the Chemical Society, Perkin Transactions : 293 -297.
-
Bordoni, A., Amaretti, A., Leonardi, A., Boschetti, E., Danesi, F., Matteuzzi, D., Roncaglia, L., Raimondi, S. and Rossi, M., 2013. Cholesterol-lowering probiotics: in vitro selection and in vivo testing of bifidobacteria. Applied Microbiology and Biotechnology 97: 8273-8281.
'Cholesterol-lowering probiotics: in vitro selection and in vivo testing of bifidobacteria ' () 97 Applied Microbiology and Biotechnology : 8273 -8281.
-
Bottacini, F., Motherway, M.O.C., Kuczynski, J., OâConnell, K.J., Serafini, F., Duranti, S., Milani, C., Turroni, F., Lugli, G.A. and Zomer, A., 2014. Comparative genomics of the Bifidobacterium breve taxon. BMC Genomics 15: 170.
'Comparative genomics of the Bifidobacterium breve taxon ' () 15 BMC Genomics : 170.
-
Choi, S.H., Lee, M.Y., Jhon, D.Y., Choi, Y.I. and Lee, J.J., 2013. Antiobesity and cholesterol-lowering effects of bifidobacteria animalis DY-64 in rats fed a high-fat/high-cholesterol diet. Korean Journal for Food Science of Animal Resources 33: 701-707.
'Antiobesity and cholesterol-lowering effects of bifidobacteria animalis DY-64 in rats fed a high-fat/high-cholesterol diet ' () 33 Korean Journal for Food Science of Animal Resources : 701 -707.
-
Dueñas-Chasco, M.T., RodrÃguez-Carvajal, M.A., Tejero-Mateo, P., Espartero, J.L., Irastorza-Iribas, A. and Gil-Serrano, A.M., 1998. Structural analysis of the exopolysaccharides produced by Lactobacillus spp. G-77. Carbohydrate Research 307: 125-133.
'Structural analysis of the exopolysaccharides produced by Lactobacillus spp ' () 307 Carbohydrate Research : 125 -133.
-
Duranti, S., Gaiani, F., Mancabelli, L., Milani, C., Grandi, A., Bolchi, A., Santoni, A., Lugli, G.A., Ferrario, C., Mangifesta, M., Viappiani, A., Bertoni, S., Vivo, V., Serafini, F., Barbaro, M.R., Fugazza, A., Barbara, G., Gioiosa, L., Palanza, P., Cantoni, A.M., Luigi deâAngelis, G., Barocelli, E., deâAngelis, N., Van Sinderen, D., Ventura, M. and Turroni, F., 2016. Elucidating the gut microbiome of ulcerative colitis: Bifidobacteria as novel microbial biomarkers. FEMS Microbiology Ecology 92: 191.
'Elucidating the gut microbiome of ulcerative colitis: Bifidobacteria as novel microbial biomarkers ' () 92 FEMS Microbiology Ecology : 191.
-
Fanning, S., Hall, L., Cronin, M., Zomer, A., MacSharry, J., Goulding, D., Motherway, M., Shanahan, F., Nally, K., Dougan, G. and Van Sinderen, D., 2012. Bifidobacterial surface-exopolysaccharide facilitates commensal-host interaction through immune modulation and pathogen protection. Proceedings of the National Academy of Sciences of the USA 109: 2108-2113.
'Bifidobacterial surface-exopolysaccharide facilitates commensal-host interaction through immune modulation and pathogen protection ' () 109 Proceedings of the National Academy of Sciences of the USA : 2108 -2113.
-
Fischer, W., 1987. âLipoteichoic acidâ of Bifidobacterium bifidum subspecies pennsylvanicum DSM 20239: a lipoglycan with monoglycerophosphate side chains. European Journal of Biochemistry 165: 639-646.
'âLipoteichoic acidâ of Bifidobacterium bifidum subspecies pennsylvanicum DSM 20239: a lipoglycan with monoglycerophosphate side chains ' () 165 European Journal of Biochemistry : 639 -646.
-
Fischer, W., Bauer, W. and Feigel, M., 1987. Analysis of the lipoteichoic-acid-like macroamphiphile from Bifidobacterium bifidum subspecies pennsylvanicum by one- and two-dimensional 1H- and 13C-NMR spectroscopy. European Journal of Biochemistry 165: 647-652.
'Analysis of the lipoteichoic-acid-like macroamphiphile from Bifidobacterium bifidum subspecies pennsylvanicum by one- and two-dimensional 1H- and 13C-NMR spectroscopy ' () 165 European Journal of Biochemistry : 647 -652.
-
Food and Agriculture Organisation/World Health Organisation (FAO/WHO), 2006. Probiotics in food â health and nutritional properties and guidelines for evaluation. Probiotics in food. Health and nutritional properties and guidelines for evaluation. FAO/WHO, Rome, Italy.
-
Gerwig, G.J., Kamerling, J.P. and Vliegenthart, J.F.G., 1978. Determination of the D and L configuration of neutral monosaccharides by high resolution capillary GLC. Carbohydrate Research 62: 349-357.
'Determination of the D and L configuration of neutral monosaccharides by high resolution capillary GLC ' () 62 Carbohydrate Research : 349 -357.
-
Habu, Y., Nagaoka, M., Yokokura, T. and Azuma, I., 1987. Structural studies of cell wall polysaccharides from Bifidobacterium breve YIT 4010 and related Bifidobacterium species. Journal of Biochemistry 102: 1423-1432.
'Structural studies of cell wall polysaccharides from Bifidobacterium breve YIT 4010 and related Bifidobacterium species ' () 102 Journal of Biochemistry : 1423 -1432.
-
Hidalgo-Cantabrana, C., López, P., Gueimonde, M., De los Reyes-Gavilán, C.G., Suárez, A., Margolles, A. and Ruas-Madiedo, P., 2012. Immune modulation capability of exopolysaccharides synthesised by lactic acid bacteria and bifidobacteria. Probiotics and Antimicrobial Proteins 4: 227-237.
'Immune modulation capability of exopolysaccharides synthesised by lactic acid bacteria and bifidobacteria ' () 4 Probiotics and Antimicrobial Proteins : 227 -237.
-
Hidalgo-Cantabrana, C., Sánchez, B., Milani, C., Ventura, M., Margolles, A. and Ruas-Madiedo, P., 2014. Genomic overview and biological functions of exopolysaccharide biosynthesis in Bifidobacterium spp. Applied and Environmental Microbiology 80: 9-18.
'Genomic overview and biological functions of exopolysaccharide biosynthesis in Bifidobacterium spp ' () 80 Applied and Environmental Microbiology : 9 -18.
-
Hughes, K.R., Harnisch, L.C., Alcon-Giner, C., Mitra, S., Wright, C.J., Ketskemety, J., Van Sinderen, D., Watson, A.J.M. and Hall, L.J., 2017. Bifidobacterium breve reduces apoptotic epithelial cell shedding in an exopolysaccharide and MyD88-dependent manner. Open Biology 7: 160155.
'Bifidobacterium breve reduces apoptotic epithelial cell shedding in an exopolysaccharide and MyD88-dependent manner ' () 7 Open Biology : 160155.
-
Inturri, R., Molinaro, A., Di Lorenzo, F., Blandino, G., Tomasello, B., Hidalgo-Cantabrana, C., De Castro, C. and Ruas-Madiedo, P., 2017. Chemical and biological properties of the novel exopolysaccharide produced by a probiotic strain of Bifidobacterium longum. Carbohydrate Polymers 174: 1172-1180.
'Chemical and biological properties of the novel exopolysaccharide produced by a probiotic strain of Bifidobacterium longum ' () 174 Carbohydrate Polymers : 1172 -1180.
-
Jones, S.E., Paynich, M.L., Kearns, D.B. and Knight, K.L., 2014. Protection from intestinal inflammation by bacterial exopolysaccharides. Journal of Immunology 192: 4813-4820.
'Protection from intestinal inflammation by bacterial exopolysaccharides ' () 192 Journal of Immunology : 4813 -4820.
-
Kato, K., Odamaki, T., Mitsuyama, E., Sugahara, H., Xiao, J.-Z. and Osawa, R., 2017. Age-related changes in the composition of gut Bifidobacterium species. Current Microbiology 74: 987-995.
'Age-related changes in the composition of gut Bifidobacterium species ' () 74 Current Microbiology : 987 -995.
-
Kleerebezem, M. and Vaughan, E.E., 2009. Probiotic and gut lactobacilli and bifidobacteria: molecular approaches to study diversity and activity. Annual Review of Microbiology 63: 269-290.
'Probiotic and gut lactobacilli and bifidobacteria: molecular approaches to study diversity and activity ' () 63 Annual Review of Microbiology : 269 -290.
-
Kohno, M., Suzuki, S., Kanaya, T., Yoshino, T., Matsuura, Y., Asada, M. and Kitamura, S., 2009. Structural characterization of the extracellular polysaccharide produced by Bifidobacterium longum JBL05. Carbohydrate Polymers 77: 351-357.
'Structural characterization of the extracellular polysaccharide produced by Bifidobacterium longum JBL05 ' () 77 Carbohydrate Polymers : 351 -357.
-
Leivers, S., Hidalgo-Cantabrana, C., Robinson, G., Margolles, A., Ruas-Madiedo, P. and Laws, A.P., 2011. Structure of the high molecular weight exopolysaccharide produced by Bifidobacterium animalis subsp. lactis IPLA-R1 and sequence analysis of its putative eps cluster. Carbohydrate Research 346: 2710-2717.
'Structure of the high molecular weight exopolysaccharide produced by Bifidobacterium animalis subsp ' () 346 Carbohydrate Research : 2710 -2717.
-
López, P., MonteserÃn, D.C., Gueimonde, M., De los Reyes-Gavilán, C.G., Margolles, A., Suárez, A. and Ruas-Madiedo, P., 2012. Exopolysaccharide-producing Bifidobacterium strains elicit different in vitro responses upon interaction with human cells. Food Research International 46: 99-107.
'Exopolysaccharide-producing Bifidobacterium strains elicit different in vitro responses upon interaction with human cells ' () 46 Food Research International : 99 -107.
-
Miremadi, F., Ayyash, M., Sherkat, F. and Stojanovska, L., 2014. Cholesterol reduction mechanisms and fatty acid composition of cellular membranes of probiotic lactobacilli and bifidobacteria. Journal of Functional Foods 9: 295-305.
'Cholesterol reduction mechanisms and fatty acid composition of cellular membranes of probiotic lactobacilli and bifidobacteria ' () 9 Journal of Functional Foods : 295 -305.
-
Nagaoka, M., Hashimoto, S., Shibata, H., Kimura, I., Kimura, K., Sawada, H. and Yokokura, T., 1996. Structure of galactan from cell walls of Bifidobacterium catenulatum YIT4016. Carbohydrate Research 281: 285-291.
'Structure of galactan from cell walls of Bifidobacterium catenulatum YIT4016 ' () 281 Carbohydrate Research : 285 -291.
-
Nagaoka, M., Muto, M., Yokokura, T. and Mutai, M., 1988. Structure of 6-deoxytalose-containing polysaccharide from the cell wall of Bifidobacterium adolescentis. Journal of Biochemistry 103: 618-621.
'Structure of 6-deoxytalose-containing polysaccharide from the cell wall of Bifidobacterium adolescentis ' () 103 Journal of Biochemistry : 618 -621.
-
Nagaoka, M., Shibata, H., Kimura, I., Hashimoto, S., Kimura, K., Sawada, H. and Yokokura, T., 1995. Structural studies on a cell wall polysaccharide from Bifidobacterium longum YIT4028. Carbohydrate Research 274: 245-249.
'Structural studies on a cell wall polysaccharide from Bifidobacterium longum YIT4028 ' () 274 Carbohydrate Research : 245 -249.
-
OâCallaghan, A. and Van Sinderen, D., 2016. Bifidobacteria and their role as members of the human gut microbiota. Frontiers in Microbiology 7: 925.
'Bifidobacteria and their role as members of the human gut microbiota ' () 7 Frontiers in Microbiology : 925.
-
Ãner, O., Aslim, B. and AydaÅ, S.B., 2014. Mechanisms of cholesterol-lowering effects of lactobacilli and bifidobacteria strains as potential probiotics with their bsh gene analysis. Journal of Molecular Microbiology and Biotechnology 24: 12-18.
'Mechanisms of cholesterol-lowering effects of lactobacilli and bifidobacteria strains as potential probiotics with their bsh gene analysis ' () 24 Journal of Molecular Microbiology and Biotechnology : 12 -18.
-
Op den Camp, H.J.M.O., Veerkamp, J.H., Oosterhof, A. and Van Halbeek, H., 1984. Structure of the lipoteichoic acids from Bifidobacterium bifidum spp. pennsylvanicum. Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism 795: 301-313.
'Structure of the lipoteichoic acids from Bifidobacterium bifidum spp ' () 795 Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism : 301 -313.
-
Orrhage, K. and Nord, C.E., 2000. Bifidobacteria and lactobacilli in human health. Drugs Under Experimental and Clinical Research 26: 95-111.
'Bifidobacteria and lactobacilli in human health ' () 26 Drugs Under Experimental and Clinical Research : 95 -111.
-
Patten, D.A., Leivers, S., Chadha, M.J., Maqsood, M., Humphreys, P.N., Laws, A.P. and Collett, A., 2014. The structure and immunomodulatory activity on intestinal epithelial cells of the EPSs isolated from Lactobacillus helveticus sp. Rosyjski and Lactobacillus acidophilus sp. 5e2. Carbohydrate Research 384: 119-127.
'The structure and immunomodulatory activity on intestinal epithelial cells of the EPSs isolated from Lactobacillus helveticus sp ' () 384 Carbohydrate Research : 119 -127.
-
Picard, C., Fioramonti, J., Francois, A., Robinson, T., Neant, F. and Matuchansky, C., 2005. Bifidobacteria as probiotic agents â physiological effects and clinical benefits. Alimentary Pharmacology and Therapeutics 22: 495-512.
'Bifidobacteria as probiotic agents â physiological effects and clinical benefits ' () 22 Alimentary Pharmacology and Therapeutics : 495 -512.
-
Ruas-Madiedo, P. and De los Reyes-Gavilan, C.G., 2005. Methods for the screening, isolation, and characterization of exopolysaccharides produced by lactic acid bacteria. Journal of Dairy Science 88: 843-856.
'Methods for the screening, isolation, and characterization of exopolysaccharides produced by lactic acid bacteria ' () 88 Journal of Dairy Science : 843 -856.
-
Ruas-Madiedo, P., Gueimonde, M., Margolles, A., De Los Reyes-Gavilán, C.G. and Salminen, S., 2006. Exopolysaccharides produced by probiotic strains modify the adhesion of probiotics and enteropathogens to human intestinal mucus. Journal of Food Protection 69: 2011-2015.
'Exopolysaccharides produced by probiotic strains modify the adhesion of probiotics and enteropathogens to human intestinal mucus ' () 69 Journal of Food Protection : 2011 -2015.
-
Saez-Lara, M.J., Gomez-Llorente, C., Plaza-Diaz, J. and Gil, A., 2015. The role of probiotic lactic acid bacteria and bifidobacteria in the prevention and treatment of inflammatory bowel disease and other related diseases: a systematic review of randomized human clinical trials. BioMed Research International 2015: 505878.
'The role of probiotic lactic acid bacteria and bifidobacteria in the prevention and treatment of inflammatory bowel disease and other related diseases: a systematic review of randomized human clinical trials ' () 2015 BioMed Research International : 505878.
-
Saitô, H., Ohki, T., Takasuka, N. and Sasaki, T., 1977. A 13C-N.M.R.-spectral study of a gel-forming, branched (1â3)-β-d-glucan, (lentinan) from Lentinus edodes, and its acid-degraded fractions. Structure, and dependence of conformation on the molecular weight. Carbohydrate Research 58: 293-305.
'A 13C-N.M.R.-spectral study of a gel-forming, branched (1â3)-β-d-glucan, (lentinan) from Lentinus edodes, and its acid-degraded fractions ' () 58 Carbohydrate Research : 293 -305.
-
Shang, N., Xu, R. and Li, P., 2013. Structure characterization of an exopolysaccharide produced by Bifidobacterium animalis RH. Carbohydrate Polymers 91: 128-134.
'Structure characterization of an exopolysaccharide produced by Bifidobacterium animalis RH ' () 91 Carbohydrate Polymers : 128 -134.
-
Shively, J., 1974. Inclusion bodies of prokaryotes. Annual Reviews in Microbiology 28: 167-188.
'Inclusion bodies of prokaryotes ' () 28 Annual Reviews in Microbiology : 167 -188.
-
Stellner, K., Saito, H. and Hakomori, S.I., 1973. Determination of aminosugar linkages in glycolipids by methylation. Aminosugar linkages of ceramide pentasaccharides of rabit erythrocytes and Forssman antigen. Archives of Biochemistry and Biophysics 155: 464-472.
'Determination of aminosugar linkages in glycolipids by methylation ' () 155 Archives of Biochemistry and Biophysics : 464 -472.
-
Tojo, R., Suárez, A., Clemente, M.G., De los Reyes-Gavilán, C.G., Margolles, A., Gueimonde, M. and Ruas-Madiedo, P., 2014. Intestinal microbiota in health and disease: role of bifidobacteria in gut homeostasis. World Journal of Gastroenterology 20: 15163-15176.
'Intestinal microbiota in health and disease: role of bifidobacteria in gut homeostasis ' () 20 World Journal of Gastroenterology : 15163 -15176.
-
Tone-Shimokawa, Y., Toida, T. and Kawashima, T., 1996. Isolation and structural analysis of polysaccharide containing galactofuranose from the cell walls of Bifidobacterium infantis. Journal of Bacteriology 178: 317-320.
'Isolation and structural analysis of polysaccharide containing galactofuranose from the cell walls of Bifidobacterium infantis ' () 178 Journal of Bacteriology : 317 -320.
-
Uemura, Y. and Matsumoto, M., 2014. Chemical structure of the cell wall-associated polysaccharide of Bifidobacterium animalis subsp. lactis LKM512. Glycoconjugate Journal 31: 555-561.
'Chemical structure of the cell wall-associated polysaccharide of Bifidobacterium animalis subsp ' () 31 Glycoconjugate Journal : 555 -561.
-
Valueva, O.A., Shashkov, A.S., Zdorovenko, E.L., Chizhov, A.O., Kiseleva, E., Novik, G. and Knirel, Y.A., 2013. Structures of cell-wall phosphate-containing glycopolymers of Bifidobacterium longum BIM B-476-D. Carbohydrate Research 373: 22-27.
'Structures of cell-wall phosphate-containing glycopolymers of Bifidobacterium longum BIM B-476-D ' () 373 Carbohydrate Research : 22 -27.
-
Veerkamp, J.H., Hoelen, G.E.J.M. and Op den Camp, H.J.M., 1983. The structure of a mannitol teichoic acid from Bifidobacterium bifidum ssp. Pennsylvanicum. BBA â General Subjects 755: 439-451.
'The structure of a mannitol teichoic acid from Bifidobacterium bifidum ssp ' () 755 BBA â General Subjects : 439 -451.
-
Wei, W., Qi, D., Zhao, H.-Z., Lu, Z.-X., Lv, F. and Bie, X., 2013. Synthesis and characterisation of galactosyl glycerol by β-galactosidase catalysed reverse hydrolysis of galactose and glycerol. Food Chemistry 141: 3085-3092.
'Synthesis and characterisation of galactosyl glycerol by β-galactosidase catalysed reverse hydrolysis of galactose and glycerol ' () 141 Food Chemistry : 3085 -3092.
-
Xu, R., Shang, N. and Li, P., 2011a. In vitro and in vivo antioxidant activity of exopolysaccharide fractions from Bifidobacterium animalis RH. Anaerobe 17: 226-231.
'In vitro and in vivo antioxidant activity of exopolysaccharide fractions from Bifidobacterium animalis RH ' () 17 Anaerobe : 226 -231.
-
Xu, R., Shen, Q., Ding, X., Gao, W. and Li, P., 2011b. Chemical characterization and antioxidant activity of an exopolysaccharide fraction isolated from Bifidobacterium animalis RH. European Food Research and Technology 232: 231-240.
'Chemical characterization and antioxidant activity of an exopolysaccharide fraction isolated from Bifidobacterium animalis RH ' () 232 European Food Research and Technology : 231 -240.
-
Zdorovenko, E.L., Kachala, V.V., Sidarenka, A.V., Izhik, A.V., Kisileva, E.P., Shashkov, A.S., Novik, G.I. and Knirel, Y.A., 2009. Structure of the cell wall polysaccharides of probiotic bifidobacteria Bifidobacterium bifidum BIM B-465. Carbohydrate Research 344: 2417-2420.
'Structure of the cell wall polysaccharides of probiotic bifidobacteria Bifidobacterium bifidum BIM B-465 ' () 344 Carbohydrate Research : 2417 -2420.
| å ¨é¨æé´ | è¿å»ä¸å¹´ | è¿å»30天 | |
|---|---|---|---|
| æè¦æµè§æ¬¡æ° | 406 | 157 | 23 |
| å ¨ææµè§æ¬¡æ° | 24 | 20 | 1 |
| PDFä¸è½½æ¬¡æ° | 22 | 14 | 3 |
Extraction of the same novel homoglycan mixture from two different strains of Bifidobacterium animalis and three strains of Bifidobacterium breve
äºBeneficial MicrobesSearch for other papers by M. Alhudhud in
Current site
Google Scholar
PubMed
Search for other papers by S. Sadiq in
Current site
Google Scholar
PubMed
Search for other papers by H.N. Ngo in
Current site
Google Scholar
PubMed
Search for other papers by C. Hidalgo-Cantabrana in
Current site
Google Scholar
PubMed
Search for other papers by P. Ruas-Madiedo in
Current site
Google Scholar
PubMed
Search for other papers by D. van Sinderen in
Current site
Google Scholar
PubMed
Search for other papers by P.N. Humphreys in
Current site
Google Scholar
PubMed
Search for other papers by A.P. Laws in
Current site
Google Scholar
PubMed
Three strains of Bifidobacterium breve (JCM 7017, JCM 7019 and JCM 2258) and two strains of Bifidobacterium animalis subsp. lactis (AD011 and A1dOxR) were grown in broth cultures or on plates, and a standard exopolysaccharide extraction method was used in an attempt to recover exocellular polysaccharides. When the extracted materials were analysed by NMR it was clear that mixtures of polysaccharides were being isolated including exopolysaccharides (EPS) cell wall polysaccharides and intracellular polysaccharides. Treatment of the cell biomass from the B. breve strains, or the B. animalis subsp. lactis AD011 strain, with aqueous sodium hydroxide provided a very similar mixture of polysaccharides but without the EPS. The different polysaccharides were partially fractionated by selective precipitation from an aqueous solution upon the addition of increasing percentages of ethanol. The polysaccharides extracted from B. breve JCM 7017 grown in HBM media supplemented with glucose (or isotopically labelled D-glucose-1-13C) were characterised using 1D and 2D-NMR spectroscopy. Addition of one volume of ethanol generated a medium molecular weight glycogen (Mw=1Ã105 Da, yield 200 mg/l). The addition of two volumes of ethanol precipitated an intimate mixture of a low molecular weight β-(1â6)-glucan and a low molecular weight β-(1â6)-galactofuranan which could not be separated (combined yield 46 mg/l). When labelled D-glucose-1-13C was used as a carbon supplement, the label was incorporated into >95% of the anomeric carbons of each polysaccharide confirming they were being synthesised in situ. Similar 1H NMR profiles were obtained for polysaccharides recovered from the cells of B. animalis subsp. lactis AD011and A1dOxR (in combination with an EPS), B. breve JCM 7017, B. breve JCM 7019, B. breve JCM 2258 and from an EPS (-ve) mutant of B. breve 7017 (a non-EPS producer).
| å ¨é¨æé´ | è¿å»ä¸å¹´ | è¿å»30天 | |
|---|---|---|---|
| æè¦æµè§æ¬¡æ° | 406 | 157 | 23 |
| å ¨ææµè§æ¬¡æ° | 24 | 20 | 1 |
| PDFä¸è½½æ¬¡æ° | 22 | 14 | 3 |
