Möchten Sie über diese Zeitschrift informiert bleiben? Klicken Sie bitte auf die Buttons, um unsere Alerts zu abonnieren.
Möchten Sie über diese Zeitschrift informiert bleiben? Klicken Sie bitte auf die Buttons, um unsere Alerts zu abonnieren.
Spermine (SPM) and its precursor putrescine (PUT), regulated by ornithine decarboxylase (ODC) and diamino-oxidase (DAO), are polyamines required for cell growth and proliferation. Only a few studies have investigated the anti-inflammatory and tumour inhibitory properties of probiotics on mucosal polyamine levels. We investigated the effects of a high concentration multistrain probiotic for human use on colonic polyamine biosynthesis in dogs. Histological sections (inflammatory bowel disease, n=10; polyposis, n=5) were assessed after receiving 112 to 225×109 lyophilised bacteria daily for 60 days at baseline (T0) and 30 days after treatment end (T90). Histology scores, expression of PUT, SPM, ODC and DAO, and a clinical activity index (CIBDAI) were compared at T0 and T90. In polyps, cellular proliferation (Ki-67 expression), and apoptosis (caspase-3 protein expression) were also evaluated. After treatment, in inflammatory bowel disease significant decreases were observed for CIBDAI (P=0.006) and histology scores (P<0.001); PUT, SPM and ODC expression increased (P<0.01). In polyps, a significant decrease in polyamine levels, ODC activity, and Ki-67, and a significant increase in caspase-3 positivity and DAO expression (P=0.005) was noted. Our results suggest potential anti-proliferative and anti-inflammatory effects of the probiotic mixture in polyps and inflammation, associated with reduced mucosal infiltration and up-regulation of PUT, SPM, and ODC levels.
Kauf
Sofortzugang erwerben (PDF-Download und unbegrenzter Online-Zugang):
Institutszugang
Melden Sie sich mit Open Athens, Shibboleth oder Ihren institutionellen Anmeldedaten an.
Persönliche Anmeldung
Melden Sie sich mit Ihrem brill.com-Konto an
Albina, J.E., Abate, J.A. and Mastrofrancesco, B., 1993. Role of ornithine as a proline precursor in healing wounds. Journal of Surgical Research 55: 97-102.
'Role of ornithine as a proline precursor in healing wounds ' () 55 Journal of Surgical Research : 97 -102.
Babbar, N. and Casero Jr., R.A., 2006. Tumor necrosis factor-alpha increases reactive oxygen species by inducing spermine oxidase in human lung epithelial cells: a potential mechanism for inflammation-induced carcinogenesis. Cancer Research 66: 11125-11130.
'Tumor necrosis factor-alpha increases reactive oxygen species by inducing spermine oxidase in human lung epithelial cells: a potential mechanism for inflammation-induced carcinogenesis ' () 66 Cancer Research : 11125 -11130.
Barry, E.L., Mott, L.A., Sandler, R.S., Ahnen, D.J. and Baron, J.A., 2011. Variants downstream of the ornithine decarboxylase gene influence risk of colorectal adenoma and aspirin chemoprevention. Cancer Prevention Research 4: 2072-2082.
'Variants downstream of the ornithine decarboxylase gene influence risk of colorectal adenoma and aspirin chemoprevention ' () 4 Cancer Prevention Research : 2072 -2082.
Boone, J., Van Hillegersberg, R., Van Diest, P.J., Offerhaus, G.J., Rinkes, I.H. and Kate, F.J., 2008. Validation of tissue microarray technology in squamous cell carcinoma of the esophagus. Virchows Archives 452: 507-514.
'Validation of tissue microarray technology in squamous cell carcinoma of the esophagus ' () 452 Virchows Archives : 507 -514.
Bussiere, F.I., Chaturvedi, R., Cheng, Y., Gobert, A.P., Asim, M., Blumberg, D.R., Xu, H., Kim, P.Y., Hacker, A., Casero Jr., R.A. and Wilson, K.T., 2005. Spermine causes loss of innate immune response to Helicobacter pylori by inhibition of inducible nitric-oxide synthase translation. Journal of Biological Chemistry 280: 2409-2412.
'Spermine causes loss of innate immune response to Helicobacter pylori by inhibition of inducible nitric-oxide synthase translation ' () 280 Journal of Biological Chemistry : 2409 -2412.
Cifone, M.G., Cinque, B., La Torre, C., Lombardi, F., Palumbo, P., Van del Rest, M.E., Vuotto, C. and Donelli, G., 2017. Complexities and pitfalls in the production of multispecies probiotics: the paradigmatic case of VSL#3 formulation and visbiome. In: Floch, M.H., Ringel, Y. and Allan Walker, W. (eds.) The microbiota in gastrointestinal pathophysiology: implications for human health, prebiotics, probiotics, and dysbiosis. Academic Press, London, UK, pp. 171-178.
'Complexities and pitfalls in the production of multispecies probiotics: the paradigmatic case of VSL#3 formulation and visbiome ', in The microbiota in gastrointestinal pathophysiology: implications for human health, prebiotics, probiotics, and dysbiosis , () 171 -178.
Coburn, L.A., Gong, X., Singh, K., Asim, M., Scull, B.P., Allaman, M.M., Williams, C.S., Rosen, M.J., Washington, M.K., Barry, D.P., Piazuelo. M.B., Casero Jr., R.A., Chaturvedi, R., Zhao. Z. and Wilson, K.T., 2012. L-arginine supplementation improves responses to injury and inflammation in dextran sulfate sodium colitis. PLoS ONE 7: e33546.
'L-arginine supplementation improves responses to injury and inflammation in dextran sulfate sodium colitis ' () 7 PLoS ONE : e33546.
Coman, D., Yaplito-Lee, J. and Boneh, A., 2008. New indications and controversies in arginine therapy. Clinical Nutrition 27: 489-496.
'New indications and controversies in arginine therapy ' () 27 Clinical Nutrition : 489 -496.
Cousin, F.J., Jouan-Lanhouet, S., Dimanche-Boitrel, M.T., Corcos, L. and Jan, G., 2012. Milk fermented by Propionibacterium freudenreichii induces apoptosis of HGT-1 human gastric cancer cells. PLoS ONE 7: e31892.
'Milk fermented by Propionibacterium freudenreichii induces apoptosis of HGT-1 human gastric cancer cells ' () 7 PLoS ONE : e31892.
Cross, R.K. and Wilson, K.T., 2003. Nitric oxide in inflammatory bowel disease. Inflammatory Bowel Disease 9: 179-189.
'Nitric oxide in inflammatory bowel disease ' () 9 Inflammatory Bowel Disease : 179 -189.
D’Agostino, L., Ciacci, C., Daniele, B., Barone, M.V., Sollazzo, R. and Mazzacca, G., 1984. Plasma diamine oxidase (DAO) and heparin. Digestive Diseases and Sciences 29: 1070-1071.
'Plasma diamine oxidase (DAO) and heparin ' () 29 Digestive Diseases and Sciences : 1070 -1071.
Day, M.J., Bilzer, T., Mansell, J., Wilcock, B., Hall, E.J., Jergens, A., Minami, T., Willard, M. and Washabau, R., 2008. Histopathological standards for the diagnosis of gastrointestinal inflammation in endoscopic biopsy samples from the dog and cat: a report from the World Small Animal Veterinary Association Gastrointestinal Standardization Group. Journal of Comparative Pathology 138, Suppl. 1: S1-S40.
'Histopathological standards for the diagnosis of gastrointestinal inflammation in endoscopic biopsy samples from the dog and cat: a report from the World Small Animal Veterinary Association Gastrointestinal Standardization Group ' () 138 Journal of Comparative Pathology : S1 -S40.
De Moreno de Leblanc, A. and Perdigón, G., 2010. The application of probiotic fermented milks in cancer and intestinal inflammation. Proceedings of the Nutrition Society 69: 421-428.
'The application of probiotic fermented milks in cancer and intestinal inflammation ' () 69 Proceedings of the Nutrition Society : 421 -428.
Di Marzio, L., Russo, F.P., D’Alò, S., Biordi, L., Ulisse, S., Amicosante, G., De Simone, C. and Cifone, M.G., 2001. Apoptotic effects of selected strains of lactic acid bacteria on a human T leukemia cell line are associated with bacterial arginine deiminase and/or sphingomyelinase activities. Nutrition Cancer 40: 185-196.
'Apoptotic effects of selected strains of lactic acid bacteria on a human T leukemia cell line are associated with bacterial arginine deiminase and/or sphingomyelinase activities ' () 40 Nutrition Cancer : 185 -196.
Famularo, G., Pieluigi, M., Coccia, R., Mastroiacovo, P. and De Simone, C., 2001. Microecology, bacterial vaginosis and probiotics: perspectives for bacteriotherapy. Medical Hypotheses 56: 421-430.
'Microecology, bacterial vaginosis and probiotics: perspectives for bacteriotherapy ' () 56 Medical Hypotheses : 421 -430.
Ferrante, A., 1985. Inhibition of human neutrophil locomotion by the polyamine oxidase-polyamine system. Immunology 54: 785-790.
'Inhibition of human neutrophil locomotion by the polyamine oxidase-polyamine system ' () 54 Immunology : 785 -790.
Fujiwara, K., Tanabe, T., Yabuuchi, M., Ueoka, R. and Tsuru, D., 2001. A monoclonal antibody against the glutaraldehyde-conjugated polyamine, putrescine: application to immunocytochemistry. Histochemistry and Cell Biology 115: 471-477.
'A monoclonal antibody against the glutaraldehyde-conjugated polyamine, putrescine: application to immunocytochemistry ' () 115 Histochemistry and Cell Biology : 471 -477.
Ganji-Arjenaki, M. and Rafieian-Kopaei, M., 2018. Probiotics are a good choice in remission of inflammatory bowel diseases: a meta-analysis and systematic review. Journal of Cellular Physiology. 233: 2091-2103.
'Probiotics are a good choice in remission of inflammatory bowel diseases: a meta-analysis and systematic review ' () 233 Journal of Cellular Physiology : 2091 -2103.
Garewal, H.S, Sampliner, R., Gerner, E., Steinbronn, K., Alberts, D. and Kendall, D., 1988. Ornithine decarboxylase activity in Barrett’s esophagus: a potential marker for dysplasia. Gastroenterology 94: 819-821.
'Ornithine decarboxylase activity in Barrett’s esophagus: a potential marker for dysplasia ' () 94 Gastroenterology : 819 -821.
Garewal, H.S., Sampliner, R., Alberts, D. and Steinbronn, K., 1989. Increase in ornithine decarboxylase activity associated with the development of dysplasia in Barrett’s oesophagus. Digestive Diseases and Sciences 34: 312-314.
'Increase in ornithine decarboxylase activity associated with the development of dysplasia in Barrett’s oesophagus ' () 34 Digestive Diseases and Sciences : 312 -314.
German, A.J., Helps, C.R., Hall, E.J. and Day, M.J., 2000. Cytokine mRNA expression in mucosal biopsies from German Shepherd dogs with small intestinal enteropathies. Digestive Diseases and Sciences 45: 7-17.
'Cytokine mRNA expression in mucosal biopsies from German Shepherd dogs with small intestinal enteropathies ' () 45 Digestive Diseases and Sciences : 7 -17.
Gerner, E.W. and Meyskens Jr., F.L., 2004. Polyamines and cancer: old molecules, new understanding. Nature Reviews Cancer 4: 781-792.
'Polyamines and cancer: old molecules, new understanding ' () 4 Nature Reviews Cancer : 781 -792.
Giardiello, F.M., Hamilton, S.R., Hylind, L.M., Yang, V.W., Tamez, P. and Casero Jr., R.A., 1997. Ornithine decarboxylase and polyamines in familial adenomatous polyposis. Cancer Research 57: 199-201.
'Ornithine decarboxylase and polyamines in familial adenomatous polyposis ' () 57 Cancer Research : 199 -201.
Gobert, A.P., Cheng, Y., Akhtar, M., Mersey, B.D., Blumberg, D.R., Cross, R.K., Chaturvedi, R., Drachenberg, C.B., Boucher, J.L., Hacker, A., Casero Jr., R.A. and Wilson, K.T., 2004. Protective role of arginase in a mouse model of colitis. Journal of Immunology 173: 2109-2117.
'Protective role of arginase in a mouse model of colitis ' () 173 Journal of Immunology : 2109 -2117.
Gown, A.M. and Willingham, M.C., 2002. Improved detection of apoptotic cells in archival paraffin sections: immunohistochemistry using antibodies to cleaved caspase 3. Journal of Histochemistry and Cytochemistry 50: 449-454.
'Improved detection of apoptotic cells in archival paraffin sections: immunohistochemistry using antibodies to cleaved caspase 3 ' () 50 Journal of Histochemistry and Cytochemistry : 449 -454.
Hall, C., Youngs, D. and Keighley, M.R., 1992. Crypt cell production rates at various sites around the colon in Wistar rats and humans. Gut 33: 1528-1531.
'Crypt cell production rates at various sites around the colon in Wistar rats and humans ' () 33 Gut : 1528 -1531.
Hwang, J.W., Baek, Y.M., Yang, K.E., Yoo, H.S., Cho, C.K., Lee, Y.W., Park, J., Eom, C.Y., Lee, Z.W., Choi, J.S. and Jang, I.S., 2013. Lactobacillus casei extract induces apoptosis in gastric cancer by inhibiting NF-κB and mTOR-mediated signaling. Integrative Cancer Therapies 12: 165-173.
'Lactobacillus casei extract induces apoptosis in gastric cancer by inhibiting NF-κB and mTOR-mediated signaling ' () 12 Integrative Cancer Therapies : 165 -173.
Igarashi, K. and Kashiwagi, K., 2010. Modulation of cellular function by polyamines. International Journal of Biochemistry and Cell Biology 42: 39-51.
'Modulation of cellular function by polyamines ' () 42 International Journal of Biochemistry and Cell Biology : 39 -51.
Jergens, A.E., Schreiner, C.A., Frank, D.E., Niyo, Y., Ahrens, F.E., Eckersall, P.D., Benson, T.J. and Evans. R., 2003. A scoring index for disease activity in canine inflammatory bowel disease. Journal of Veterinary Internal Medicine 17: 291-297.
'A scoring index for disease activity in canine inflammatory bowel disease ' () 17 Journal of Veterinary Internal Medicine : 291 -297.
Li, H., Meininger, C.J., Kelly, K.A., Hawker Jr., J.R., Morris Jr., S.M. and Wu, G., 2002. Activities of arginase I and II are limiting for endothelial cell proliferation. American Journal of Physiology Regulatory. Integrative and Comparative Physiology 282: R64-R69.
'Activities of arginase I and II are limiting for endothelial cell proliferation ' () 282 American Journal of Physiology Regulatory. Integrative and Comparative Physiology : R64 -R69.
Li, L., Rao, J.N., Guo, X., Liu, L., Santora, R., Bass, B.L. and Wang, J.Y., 2001. Polyamine depletion stabilizes p53 resulting in inhibition of normal intestinal epithelial cell proliferation. American Journal of Physiology Cell Physiology 281: C941-C953.
'Polyamine depletion stabilizes p53 resulting in inhibition of normal intestinal epithelial cell proliferation ' () 281 American Journal of Physiology Cell Physiology : C941 -C953.
Luk, G.D. and Baylin, S.B., 1984. Ornithine decarboxylase as a biologic marker in familial colonic polyposis. New England Journal of Medicine 311: 80-83.
'Ornithine decarboxylase as a biologic marker in familial colonic polyposis ' () 311 New England Journal of Medicine : 80 -83.
McGarrity, T.J., Peiffer, L.P., Bartholomew, M.J. and Pegg, A.E., 1990. Colonic polyamine content and ornithine decarboxylase activity as markers of adenomas. Cancer 66: 1539-1543.
'Colonic polyamine content and ornithine decarboxylase activity as markers of adenomas ' () 66 Cancer : 1539 -1543.
Meyskens, F.L., McLaren, C.E., Pelot, D., Fujikawa-Brooks, S., Carpenter, P.M., Hawk, E., Kelloff, G., Lawson, M.J., Kidao, J., McCracken, J., Albers, C.G., Ahnen, D.J., Turgeon, D.K., Goldschmid, S., Lance, P., Hagedorn, C.H., Gillen, D.L. and Gerner, E.W., 2008. Difluoromethylornithine plus sulindac for the prevention of sporadic colorectal adenomas: a randomized placebo-controlled, double-blind trial. Cancer Prevention and Research 1: 9-11.
'Difluoromethylornithine plus sulindac for the prevention of sporadic colorectal adenomas: a randomized placebo-controlled, double-blind trial ' () 1 Cancer Prevention and Research : 9 -11.
Miller, R.T., Swanson, P.E. and Wick, M.R., 2000. Fixation and epitope retrieval in diagnostic immunohistochemistry: a concise review with practical considerations. Applied Immunohistochemistry and Molecular Morphology 8: 228-235.
'Fixation and epitope retrieval in diagnostic immunohistochemistry: a concise review with practical considerations ' () 8 Applied Immunohistochemistry and Molecular Morphology : 228 -235.
Mori, M., 2007. Regulation of nitric oxide synthesis and apoptosis by arginase and arginine recycling. Journal of Nutrition 137: 1616S-1620S.
'Regulation of nitric oxide synthesis and apoptosis by arginase and arginine recycling ' () 137 Journal of Nutrition : 1616S -1620S.
Narisawa, T., Takahashi, M., Niwa, M., Koyama, H., Kotanagi, H., Kusaka, N., Yamazaki, Y., Nagasawa, O., Koyama, K., Wakizaka, A. and Fukaura, Y., 1989. Increased mucosal ornithine decarboxylase activity in large bowel with multiple tumours, adenocarcinoma and adenoma. Cancer 63: 1572-1576.
'Increased mucosal ornithine decarboxylase activity in large bowel with multiple tumours, adenocarcinoma and adenoma ' () 63 Cancer : 1572 -1576.
Orlando, A., Messa, C., Linsalata, M., Cavallini, A. and Russo, F., 2009. Effects of Lactobacillus rhamnosus GG on proliferation and polyamine metabolism in HGC-27 human gastric and DLD-1 colonic cancer cell lines. Immunopharmacology and Immunotoxicology 31: 108-116.
'Effects of Lactobacillus rhamnosus GG on proliferation and polyamine metabolism in HGC-27 human gastric and DLD-1 colonic cancer cell lines ' () 31 Immunopharmacology and Immunotoxicology : 108 -116.
Pegg, A.E. and McCann, P.P., 1982. Polyamines metabolism and function. American Journal of Physiology Cell Physiology 243: C212-C221.
'Polyamines metabolism and function ' () 243 American Journal of Physiology Cell Physiology : C212 -C221.
Pegg, A.E., 2009. Mammalian polyamine metabolism and function. IUBMB Life 61: 880-894.
'Mammalian polyamine metabolism and function ' () 61 IUBMB Life : 880 -894.
Pegg, A.E., Shantz, L.M. and Coleman, C.S., 1995. Ornithine decarboxylase as a target for chemoprevention. Journal of Cellular Biochemistry 59, Suppl. 22: 132-138.
'Ornithine decarboxylase as a target for chemoprevention ' () 59 Journal of Cellular Biochemistry : 132 -138.
Peran, L., Camuesco, D., Comalada, M., Nieto, A., Concha, A., Diaz-Ropero, M.P., Olivares, M., Xaus, J., Zarzuelo, A. and Galvez, J., 2005. Preventative effects of a probiotic, Lactobacillus salivarius ssp. salivarius, in the TNBS model of rat colitis. World Journal of Gastroenterology 11: 5185-5192.
'Preventative effects of a probiotic, Lactobacillus salivarius ssp. salivarius, in the TNBS model of rat colitis ' () 11 World Journal of Gastroenterology : 5185 -5192.
Pessi, T., Sutas, Y., Saxelin, M., Kallioinen, H. and Isolauri, E., 1999. Antiproliferative effects of homogenates derived from five strains of candidate probiotic bacteria. Applied and Environmental Microbiology 65: 4725-4728.
'Antiproliferative effects of homogenates derived from five strains of candidate probiotic bacteria ' () 65 Applied and Environmental Microbiology : 4725 -4728.
Rao, J.N., Li, J., Li, L., Bass, B.L. and Wang, J.Y., 1999. Differentiated intestinal epithelial cells exhibit increased migration through polyamines and myosin II. American Journal of Physiology – Gastrointestinal and Liver Physiology 277: G1149-G1158.
'Differentiated intestinal epithelial cells exhibit increased migration through polyamines and myosin II ' () 277 American Journal of Physiology – Gastrointestinal and Liver Physiology : G1149 -G1158.
Rao, J.N., Li, L., Golovina, V.A., Platoshyn, O., Strauch, E.D., Yuan, J.X. and Wang, J.Y., 2001. Ca2+-RhoA signaling pathway required for polyamine-dependent intestinal epithelial cell migration. American Journal of Physiology – Cell Physiology 280: C993-C1007.
'Ca2+-RhoA signaling pathway required for polyamine-dependent intestinal epithelial cell migration ' () 280 American Journal of Physiology – Cell Physiology : C993 -C1007.
Rhoads, J.M., Chen, W., Gookin, J., Wu, G.Y., Fu, Q., Blikslager, A.T., Rippe, R.A., Argenzio, R.A., Cance, W.G., Weaver, E.M. and Romer, L.H., 2004. Arginine stimulates intestinal cell migration through a focal adhesion kinase dependent mechanism. Gut 53: 514-522.
'Arginine stimulates intestinal cell migration through a focal adhesion kinase dependent mechanism ' () 53 Gut : 514 -522.
Rial, N.S., Meyskens, F.L., Gerner, E.W., 2009. Polyamines as mediators of APC-dependent intestinal carcinogenesis and cancer chemoprevention. Essays in Biochemistry 46: 111-124.
'Polyamines as mediators of APC-dependent intestinal carcinogenesis and cancer chemoprevention ' () 46 Essays in Biochemistry : 111 -124.
Rogers, A.C., McDermott, F.D., Mohan, H.M., O’Connell, P.R., Winter, D.C. and Baird, A.W., 2015. The effects of polyamines on human colonic mucosal function. European Journal of Pharmacology 764: 157-163.
'The effects of polyamines on human colonic mucosal function ' () 764 European Journal of Pharmacology : 157 -163.
Rossi, G., Cerquetella, M., Pengo, G., Mari, S., Balint, E., Bassotti, G. and Manolescu, N., 2015. Immunohistochemical expression of ornithine decarboxylase, diamine oxidase, putrescine, and spermine in normal canine enterocolic mucosa, in chronic colitis, and in colorectal cancer. BioMed Research International 2015: 172756.
'Immunohistochemical expression of ornithine decarboxylase, diamine oxidase, putrescine, and spermine in normal canine enterocolic mucosa, in chronic colitis, and in colorectal cancer ' () 2015 BioMed Research International : 172756.
Rossi, G., Pengo, G., Caldin, M., Palumbo Piccionello, A., Steiner, J.M., Cohen, N.D., Jergens, A.E. and Suchodolski, J.S., 2014. Comparison of microbiological, histological, and immunomodulatory parameters in response to treatment with either combination therapy with prednisone and metronidazole or probiotic strains in dogs with idiopathic inflammatory bowel disease. PLoS ONE 9: e94699.
'Comparison of microbiological, histological, and immunomodulatory parameters in response to treatment with either combination therapy with prednisone and metronidazole or probiotic strains in dogs with idiopathic inflammatory bowel disease ' () 9 PLoS ONE : e94699.
Sharma, J.N., Al-Omran, A. and Parvathy, S.S., 2007. Role of nitric oxide in inflammatory diseases. Inflammopharmacology 15: 252-259.
'Role of nitric oxide in inflammatory diseases ' () 15 Inflammopharmacology : 252 -259.
Simpson, K.W. and Jergens, A.E., 2011. Pitfalls and progress in the diagnosis and management of canine inflammatory bowel disease. Veterinary Clinics of North America – Small Animals Practice 41: 381-398.
'Pitfalls and progress in the diagnosis and management of canine inflammatory bowel disease ' () 41 Veterinary Clinics of North America – Small Animals Practice : 381 -398.
Steege, J.C., Forget, P.P. and Buurman, W.A., 1999. Oral spermine administration inhibits nitric oxide-mediated intestinal damage and levels of systemic inflammatory mediators in a mouse endotoxin model. Shock 11: 115-119.
'Oral spermine administration inhibits nitric oxide-mediated intestinal damage and levels of systemic inflammatory mediators in a mouse endotoxin model ' () 11 Shock : 115 -119.
Thomas, T. and Thomas, T.J., 2001. Polyamines in cell growth and cell death: molecular mechanisms and therapeutic applications. Cellular and Molecular Life Sciences 58: 244-258.
'Polyamines in cell growth and cell death: molecular mechanisms and therapeutic applications ' () 58 Cellular and Molecular Life Sciences : 244 -258.
Van Vliet, M.J., Harmsen, H.J.M., De Bont, E.S.J.M. and Tissing, W.J.E., 2010. The role of intestinal microbiota in the development and severity of chemotherapy-induced mucositis. PLoS Pathogens 6: e1000879.
'The role of intestinal microbiota in the development and severity of chemotherapy-induced mucositis ' () 6 PLoS Pathogens : e1000879.
Wang, J.Y. and Johnson, L.R., 1991. Polyamines and ornithine decarboxylase during repair of duodenal mucosa after stress in rats. Gastroenterology 100: 333-343.
'Polyamines and ornithine decarboxylase during repair of duodenal mucosa after stress in rats ' () 100 Gastroenterology : 333 -343.
Wang, J.Y., Johnson, L.R., Tsai, Y.H. and Castro, G.A., 1991. Mucosal ornithine decarboxylase, polyamines, and hyperplasia in infected intestine. American Journal of Physiology 260: G45-G51.
'Mucosal ornithine decarboxylase, polyamines, and hyperplasia in infected intestine ' () 260 American Journal of Physiology : G45 -G51.
Zaletok, S., Alexandrova, N., Berdynskykh, N., Ignatenko, N., Gogol, S., Orlovsky, O., Tregubova, N., Gerner, E. and Chekhun, V., 2004. Role of polyamines in the function of nuclear transcription factor NF-kappaB in breast cancer cells. Experimental Oncology 26: 221-225.
'Role of polyamines in the function of nuclear transcription factor NF-kappaB in breast cancer cells ' () 26 Experimental Oncology : 221 -225.
Zhang, M., Caragine, T., Wang, H., Cohen, P.S., Botchkina, G., Soda, K., Bianchi, M., Ulrich, P., Cerami, A., Sherry, B. and Tracey, K.J., 1997. Spermine inhibits proinflammatory cytokine synthesis in human mononuclear cells: a counterregulatory mechanism that restrains the immune response. Journal of Experimental Medicine 185: 1759-1768.
'Spermine inhibits proinflammatory cytokine synthesis in human mononuclear cells: a counterregulatory mechanism that restrains the immune response ' () 185 Journal of Experimental Medicine : 1759 -1768.
| Insgesamt | Letzte 365 Tage | In den letzten 30 Tagen | |
|---|---|---|---|
| Aufrufe von Kurzbeschreibungen | 884 | 231 | 17 |
| Gesamttextansichten | 72 | 14 | 1 |
| PDF-Downloads | 74 | 12 | 3 |
Spermine (SPM) and its precursor putrescine (PUT), regulated by ornithine decarboxylase (ODC) and diamino-oxidase (DAO), are polyamines required for cell growth and proliferation. Only a few studies have investigated the anti-inflammatory and tumour inhibitory properties of probiotics on mucosal polyamine levels. We investigated the effects of a high concentration multistrain probiotic for human use on colonic polyamine biosynthesis in dogs. Histological sections (inflammatory bowel disease, n=10; polyposis, n=5) were assessed after receiving 112 to 225×109 lyophilised bacteria daily for 60 days at baseline (T0) and 30 days after treatment end (T90). Histology scores, expression of PUT, SPM, ODC and DAO, and a clinical activity index (CIBDAI) were compared at T0 and T90. In polyps, cellular proliferation (Ki-67 expression), and apoptosis (caspase-3 protein expression) were also evaluated. After treatment, in inflammatory bowel disease significant decreases were observed for CIBDAI (P=0.006) and histology scores (P<0.001); PUT, SPM and ODC expression increased (P<0.01). In polyps, a significant decrease in polyamine levels, ODC activity, and Ki-67, and a significant increase in caspase-3 positivity and DAO expression (P=0.005) was noted. Our results suggest potential anti-proliferative and anti-inflammatory effects of the probiotic mixture in polyps and inflammation, associated with reduced mucosal infiltration and up-regulation of PUT, SPM, and ODC levels.
| Insgesamt | Letzte 365 Tage | In den letzten 30 Tagen | |
|---|---|---|---|
| Aufrufe von Kurzbeschreibungen | 884 | 231 | 17 |
| Gesamttextansichten | 72 | 14 | 1 |
| PDF-Downloads | 74 | 12 | 3 |