Lactobacillus pentosus B281 and Lactobacillus plantarum B282 are two Lactobacillus strains previously isolated from fermented table olives. Both strains were found to possess probiotic properties and displayed desirable technological characteristics for application as starters in novel functional food production. In the present study the anti-proliferative and immunostimulatory activities of the two strains were investigated. Firstly, we demonstrated that live L. pentosus B281 and L. plantarum B282 significantly inhibited the growth of human colon cancer cells (Caco-2) in a time- and dose-dependent manner. By employing the air pouch system in mice, we showed that administration of both strains led to a rapid and statistically significant infiltration of leukocytes in the air pouch exudates. The phenotypical characterisation of the recruited immune cells was performed by flow cytometry analysis. We demonstrated that the majority of the infiltrated leukocytes were neutrophils. Finally by using the Mouse Cytokine Array Panel A Detection Antibody cocktail, we showed that both strains induced the expression of granulocyte-colony stimulating factor, interleukin (IL)-1α, IL-1β, IL-6, chemokine (C-X-C motif) ligand (CXCL)-1, chemokine (C-C motif) ligand (CCL)-3, CCL-4, and CXCL-2 and diminished the expression levels of soluble intercellular adhesion molecule, macrophage colony-stimulating factor and metallopeptidase inhibitor 1. Our results showed that both strains display anti-proliferative and immunostimulatory properties equal or even better in some cases than those of established and commonly used probiotic strains. These findings further support the probiotic character of the two strains.
Purchase
Buy instant access (PDF download and unlimited online access):
Institutional Login
Log in with Open Athens, Shibboleth, or your institutional credentials
Personal login
Log in with your brill.com account
Argyri, A.A., Nisiotou, A.A., Mallouchos, A., Panagou, E.Z. and Tassou, C.C., 2014. Performance of two potential probiotic Lactobacillus strains from the olive microbiota as starters in the fermentation of heat shocked green olives. International Journal of Food Microbiology 171: 68-76.
'Performance of two potential probiotic Lactobacillus strains from the olive microbiota as starters in the fermentation of heat shocked green olives ' () 171 International Journal of Food Microbiology : 68 -76.
Argyri, A.A., Zoumpopoulou, G., Karatzas, K.A., Tsakalidou, E., Nychas, G.J., Panagou, E.Z. and Tassou, C.C., 2013. Selection of potential probiotic lactic acid bacteria from fermented olives by in vitro tests. Food Microbiology 33: 282-291.
'Selection of potential probiotic lactic acid bacteria from fermented olives by in vitro tests ' () 33 Food Microbiology : 282 -291.
Barton, B.E. and Jackson, J.V., 1993. Protective role of interleukin-6 in the lipopolysaccharide-galactosamine septic shock model. Infection and Immunity 61: 1496-1499.
'Protective role of interleukin-6 in the lipopolysaccharide-galactosamine septic shock model ' () 61 Infection and Immunity : 1496 -1499.
Bermudez-Brito, M., Plaza-Díaz, J., Muñoz-Quezada, S., Gómez-Llorente, C. and Gil, A., 2012. Probiotic mechanisms of action. Annals of Nutrition and Metabolism 61: 160-174.
'Probiotic mechanisms of action ' () 61 Annals of Nutrition and Metabolism : 160 -174.
Blana, V., Grounta, A., Tassou, C.C., Nychas, G.J. and Panagou, E., 2014. Inoculated fermentation of green olives with potential probiotic Lactobacillus pentosus and Lactobacillus plantarum starter cultures isolated from industrially fermented olives. Food Microbiology 38: 208-218.
'Inoculated fermentation of green olives with potential probiotic Lactobacillus pentosus and Lactobacillus plantarum starter cultures isolated from industrially fermented olives ' () 38 Food Microbiology : 208 -218.
Chon, H., Choi, B., Lee, E., Lee, S. and Jeong, G., 2009. Immunomodulatory effects of specific bacterial components of Lactobacillus plantarum KFCC11389P on the murine macrophage cell line RAW 264.7. Journal of Applied Microbiology 107: 1588-1597.
'Immunomodulatory effects of specific bacterial components of Lactobacillus plantarum KFCC11389P on the murine macrophage cell line RAW 264.7 ' () 107 Journal of Applied Microbiology : 1588 -1597.
Crispie, F., Alonso-Gomez, M., O’Loughlin, C., Klostermann, K., Flynn, J., Arkins, S., Meaney, W., Paul Ross, R. and Hill, C., 2008. Intramammary infusion of a live culture for treatment of bovine mastitis: effect of live Lactococci on the mammary immune response. Journal of Dairy Research 75: 374-384.
'Intramammary infusion of a live culture for treatment of bovine mastitis: effect of live Lactococci on the mammary immune response ' () 75 Journal of Dairy Research : 374 -384.
Dimitrellou, D., Kandylis, P., Sidira, M., Koutinas, A.A. and Kourkoutas, Y., 2014. Free and immobilized Lactobacillus casei ATCC 393 on whey protein as starter cultures for probiotic Feta-type cheese production. Journal of Dairy Science 97: 4675-4685.
'Free and immobilized Lactobacillus casei ATCC 393 on whey protein as starter cultures for probiotic Feta-type cheese production ' () 97 Journal of Dairy Science : 4675 -4685.
Dong, H., Rowland, I., Tuohy, K.M., Thomas, L.V. and Yaqoob, P., 2010. Selective effects of Lactobacillus casei Shirota on T cell activation, natural killer cell activity and cytokine production. Clinical and Experimental Immunology 161: 378-388.
'Selective effects of Lactobacillus casei Shirota on T cell activation, natural killer cell activity and cytokine production ' () 161 Clinical and Experimental Immunology : 378 -388.
Doulgeraki, A.I., Pramateftaki, P., Argyri, A.A., Nychas, G.J., Tassou, C.C. and Panagou, E.Z., 2013. Molecular characterization of lactic acid bacteria isolated from industrially fermented Greek table olives. LWT – Food Science and Technology 50: 353-356.
'Molecular characterization of lactic acid bacteria isolated from industrially fermented Greek table olives ' () 50 LWT – Food Science and Technology : 353 -356.
Food and Agriculture Organization of the United Nations/World Health Organization (FAO/WHO), 2002. Guidelines for the evaluation of probiotics in food. Report of a Joint FAO/WHO working group on drafting guidelines for the evaluation of probiotics in food. Available at: http://tinyurl.com/ou88oa2.
Gabay, C., 2006. Interleukin-6 and chronic inflammation. Arthritis Research and Therapy 8, Suppl. 2: S3.
'Interleukin-6 and chronic inflammation ' () 8 Arthritis Research and Therapy : S3.
Galdeano, C.M., De Moreno de LeBlanc, A., Vinderola, G., Bonet, M.E. and Perdigon, G., 2007. Proposed model: mechanisms of immunomodulation induced by probiotic bacteria. Clinical and Vaccine Immunology 14: 485-492.
'Proposed model: mechanisms of immunomodulation induced by probiotic bacteria ' () 14 Clinical and Vaccine Immunology : 485 -492.
Hershko, D.D., Robb, B.W., Luo, G.J., Paxton, J.H. and Hasselgren, P.O., 2003. Interleukin-6 induces thermotolerance in cultured Caco-2 cells independent of the heat shock response. Cytokine 21: 1-9.
'Interleukin-6 induces thermotolerance in cultured Caco-2 cells independent of the heat shock response ' () 21 Cytokine : 1 -9.
Jiang, Y., Li, L., Sun, H., Shan, Y., Liu, Y., Li, L., Qu, B. and Man, C., 2016. Induction of cytokines via NF-κB and p38 MAP kinase signalling pathways associated with the immunomodulation by Lactobacillus plantarum NDC 75017 in vitro and in vivo. Journal of Functional Foods 20: 215-225.
'Induction of cytokines via NF-κB and p38 MAP kinase signalling pathways associated with the immunomodulation by Lactobacillus plantarum NDC 75017 in vitro and in vivo ' () 20 Journal of Functional Foods : 215 -225.
Kotzamanidis, C., Kourelis, A., Litopoulou-Tzanetaki, E., Tzanetakis, N. and Yiangou, M., 2010. Evaluation of adhesion capacity, cell surface traits and immunomodulatory activity of presumptive probiotic Lactobacillus strains. International Journal of Food Microbiology 140: 154-163.
'Evaluation of adhesion capacity, cell surface traits and immunomodulatory activity of presumptive probiotic Lactobacillus strains ' () 140 International Journal of Food Microbiology : 154 -163.
Kourelis, A., Zinonos, I., Kakagianni, M., Christidou, A., Christoglou, N., Yiannaki, E., Testa, T., Kotzamanidis, C., Litopoulou-Tzanetaki, E., Tzanetakis, N. and Yiangou, M., 2010. Validation of the dorsal air pouch model to predict and examine immunostimulatory responses in the gut. Journal of Applied Microbiology 108: 274-284.
'Validation of the dorsal air pouch model to predict and examine immunostimulatory responses in the gut ' () 108 Journal of Applied Microbiology : 274 -284.
Li, J., Zhang, W., Wang, C., Yu, Q., Dai, R. and Pei, X., 2012. Lactococcus lactis expressing food-grade β-galactosidase alleviates lactose intolerance symptoms in post-weaning Balb/c mice. Applied Microbiology and Biotechnology 96: 1499-1506.
'Lactococcus lactis expressing food-grade β-galactosidase alleviates lactose intolerance symptoms in post-weaning Balb/c mice ' () 96 Applied Microbiology and Biotechnology : 1499 -1506.
Ma, E.L., Choi, Y.J., Choi, J., Pothoulakis, C., Rhee, S.H. and Im, E., 2010. The anticancer effect of probiotic Bacillus polyfermenticuson human colon cancer cells is mediated through ErbB2 and ErbB3 inhibition. International Journal of Cancer 127: 780-790.
'The anticancer effect of probiotic Bacillus polyfermenticuson human colon cancer cells is mediated through ErbB2 and ErbB3 inhibition ' () 127 International Journal of Cancer : 780 -790.
Orlando, A., Refolo, M.G., Messa, C., Amati, L., Lavermicocca, P., Guerra, V. and Russo, F., 2012. Antiproliferative and proapoptotic effects of viable or heat-killed Lactobacillus paracasei IMPC2.1 and Lactobacillus rhamnosus GG in HGC-27 gastric and DLD-1 colon cell lines. Nutrition and Cancer 64: 1103-1111.
'Antiproliferative and proapoptotic effects of viable or heat-killed Lactobacillus paracasei IMPC2.1 and Lactobacillus rhamnosus GG in HGC-27 gastric and DLD-1 colon cell lines ' () 64 Nutrition and Cancer : 1103 -1111.
Saxami, G., Karapetsas, A., Lamprianidou, E., Kotsianidis, I., Chlichlia, A., Tassou, C.C., Vassilis Zoumpourlis, V. and Galanis, A., 2016b. Two potential probiotic Lactobacillus strains isolated from olive microbiota exhibit adhesion and anti-proliferative effects in cancer cell lines. Journal of Functional Foods 24: 461-471.
'Two potential probiotic Lactobacillus strains isolated from olive microbiota exhibit adhesion and anti-proliferative effects in cancer cell lines ' () 24 Journal of Functional Foods : 461 -471.
Saxami, G., Papadopoulou, O.S., Chorianopoulos, N., Kourkoutas, Y., Tassou, C.C. and Galanis, A., 2016a. Molecular detection of two potential probiotic lactobacilli strains and evaluation of their performance as starter adjuncts in yogurt production. International Journal of Molecular Sciences 17: 668.
'Molecular detection of two potential probiotic lactobacilli strains and evaluation of their performance as starter adjuncts in yogurt production ' () 17 International Journal of Molecular Sciences : 668.
Sedgwick, A.D. and Lees, P., 1986. Studies of eicosanoid production in the air pouch model of synovial inflammation. Agents and Actions 18: 429-438.
'Studies of eicosanoid production in the air pouch model of synovial inflammation ' () 18 Agents and Actions : 429 -438.
Seow, S.W., Cai, S., Rahmat, J.N., Bay, B.H., Lee, Y.K., Chan, Y.H. and Mahendran, R., 2010. Lactobacillus rhamnosus GG induces tumor regression in mice bearing orthotopic bladder tumors. Cancer Science 101: 751-758.
'Lactobacillus rhamnosus GG induces tumor regression in mice bearing orthotopic bladder tumors ' () 101 Cancer Science : 751 -758.
Shanley, T.P., Foreback, J.L., Pemick, D.G., Ulich, T.R., Kunkel, S.L. and Ward, P.A., 1997. Regulatory effects of interleukin-6 in immunoglobulin G immune-complex-induced lung injury. American Journal of Pathology 151: 193-203.
'Regulatory effects of interleukin-6 in immunoglobulin G immune-complex-induced lung injury ' () 151 American Journal of Pathology : 193 -203.
Shida, K. and Nanno, M., 2008. Probiotics and immunology: separating the wheat from the chaff. Trends in Immunology 11: 565-573.
'Probiotics and immunology: separating the wheat from the chaff ' () 11 Trends in Immunology : 565 -573.
Sidira, M., Karapetsas, A., Galanis, A., Kanellaki, M. and Kourkoutas, Y., 2014. Effective survival of immobilized Lactobacillus casei during ripening and heat treatment of probiotic dry-fermented sausages and investigation of the microbial dynamics. Meat Science 96: 948-955.
'Effective survival of immobilized Lactobacillus casei during ripening and heat treatment of probiotic dry-fermented sausages and investigation of the microbial dynamics ' () 96 Meat Science : 948 -955.
Sidira, M., Saxami, G., Dimitrellou, D., Santarmaki, V., Galanis, A. and Kourkoutas, Y., 2013. Monitoring survival of Lactobacillus casei ATCC 393 in probiotic yogurts using an efficient molecular tool. Journal of Dairy Science 96: 3369-3377.
'Monitoring survival of Lactobacillus casei ATCC 393 in probiotic yogurts using an efficient molecular tool ' () 96 Journal of Dairy Science : 3369 -3377.
Taverniti, V. and Guglielmetti, S., 2011. The immunomodulatory properties of probiotic microorganisms beyond their viability (ghost probiotics: proposal of paraprobiotic concept). Genes and Nutrition 6: 261-274.
'The immunomodulatory properties of probiotic microorganisms beyond their viability (ghost probiotics: proposal of paraprobiotic concept) ' () 6 Genes and Nutrition : 261 -274.
Tiptiri-Kourpeti, A., Spyridopoulou, K., Santarmaki, V., Aindelis, G., Tompoulidou, E., Lamprianidou, E.E., Saxami, G., Ypsilantis, P., Lampri, E.S., Simopoulos, C., Kotsianidis, I., Galanis, A., Kourkoutas, Y., Dimitrellou, D. and Chlichlia, K., 2016. Lactobacillus casei exerts anti-proliferative effects accompanied by apoptotic cell death and up-regulation of TRAIL in colon carcinoma cells. PLoS ONE 11: e0147960.
'Lactobacillus casei exerts anti-proliferative effects accompanied by apoptotic cell death and up-regulation of TRAIL in colon carcinoma cells ' () 11 PLoS ONE : e0147960.
Uccello, M., Malaguarnera, G., Basile, F., D’agata, V., Malaguarnera, M., Bertino, G., Vacante, M., Drago, F. and Biondi, A., 2012. Potential role of probiotics on colorectal cancer prevention. BMC Surgery 12, Suppl. 1: S35.
'Potential role of probiotics on colorectal cancer prevention ' () 12 BMC Surgery : S35.
Vaarala, O., 2003. Immunological effects of probiotics with special reference to lactobacilli. Clinical and Experimental Allergy 33: 1634-1640.
'Immunological effects of probiotics with special reference to lactobacilli ' () 33 Clinical and Experimental Allergy : 1634 -1640.
Varankovich, N.V., Nickerson, M.T. and Korber, D.R., 2015. Probiotic-based strategies for therapeutic and prophylactic use against multiple gastrointestinal diseases. Frontiers in Microbiology 6: 685.
'Probiotic-based strategies for therapeutic and prophylactic use against multiple gastrointestinal diseases ' () 6 Frontiers in Microbiology : 685.
Zhong, L., Zhang, X. and Covasa, M., 2014. Emerging roles of lactic acid bacteria in protection against colorectal cancer. World Journal of Gastroenterology 23: 7878-7886.
'Emerging roles of lactic acid bacteria in protection against colorectal cancer ' () 23 World Journal of Gastroenterology : 7878 -7886.
Zocco, M.A., Dal Verme, L.Z., Cremonini, F., Piscaglia, A.C., Nista, E.C., Candelli, M., Novi, M., Rigante, D., Cazzato, I.A., Ojetti, V., Armuzzi, A., Gasbarrini, G. and Gasbarrini, A., 2006. Efficacy of Lactobacillus GG in maintaining remission of ulcerative colitis. Alimentary Pharmacology and Therapeutics 23: 1567-1574.
'Efficacy of Lactobacillus GG in maintaining remission of ulcerative colitis ' () 23 Alimentary Pharmacology and Therapeutics : 1567 -1574.
| All Time | Past 365 days | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 593 | 208 | 28 |
| Full Text Views | 15 | 2 | 0 |
| PDF Views & Downloads | 9 | 0 | 0 |
Lactobacillus pentosus B281 and Lactobacillus plantarum B282 are two Lactobacillus strains previously isolated from fermented table olives. Both strains were found to possess probiotic properties and displayed desirable technological characteristics for application as starters in novel functional food production. In the present study the anti-proliferative and immunostimulatory activities of the two strains were investigated. Firstly, we demonstrated that live L. pentosus B281 and L. plantarum B282 significantly inhibited the growth of human colon cancer cells (Caco-2) in a time- and dose-dependent manner. By employing the air pouch system in mice, we showed that administration of both strains led to a rapid and statistically significant infiltration of leukocytes in the air pouch exudates. The phenotypical characterisation of the recruited immune cells was performed by flow cytometry analysis. We demonstrated that the majority of the infiltrated leukocytes were neutrophils. Finally by using the Mouse Cytokine Array Panel A Detection Antibody cocktail, we showed that both strains induced the expression of granulocyte-colony stimulating factor, interleukin (IL)-1α, IL-1β, IL-6, chemokine (C-X-C motif) ligand (CXCL)-1, chemokine (C-C motif) ligand (CCL)-3, CCL-4, and CXCL-2 and diminished the expression levels of soluble intercellular adhesion molecule, macrophage colony-stimulating factor and metallopeptidase inhibitor 1. Our results showed that both strains display anti-proliferative and immunostimulatory properties equal or even better in some cases than those of established and commonly used probiotic strains. These findings further support the probiotic character of the two strains.
| All Time | Past 365 days | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 593 | 208 | 28 |
| Full Text Views | 15 | 2 | 0 |
| PDF Views & Downloads | 9 | 0 | 0 |