Streptococcus thermophilus NCIMB 41856 ameliorates signs of colitis in an animal model of inflammatory bowel disease
In: Beneficial MicrobesSearch for other papers by J.R. Bailey in
Current site
Google Scholar
Search for other papers by V. Vince in
Current site
Google Scholar
Search for other papers by N.A. Williams in
Current site
Google Scholar
Search for other papers by T.A. Cogan in
Current site
Google Scholar
Treatment of inflammatory bowel disease (IBD) is mainly based on suppression of symptoms, often with numerous side effects. Trials of probiotics in IBD have frequently produced disappointing results. The majority of probiotics are unusual, since they do not require iron for growth, unlike many bacteria resident in the intestine. The IBD intestine is iron-rich due to bleeding and use of oral iron supplements; conventional probiotics would be rapidly outcompeted. We have evaluated an iron-responsive Streptococcus thermophilus strain for its potential to reduce signs of colitis. Efficacy of S. thermophilus was evaluated in the dextran sodium sulphate mouse model of colitis. Treated animals were given 1×108 cfu S. thermophilus per day and clinical observations were taken daily. At termination, gross and histopathological signs of disease, cellular infiltration, location of bacteria, and cytokine expression in the intestine were determined. S. thermophilus delayed onset of colitis and reduced clinical signs of disease, including bodyweight loss and gastrointestinal bleeding. It reduced bacterial translocation into the colonic tissue. Increased numbers of CD8+ intraepithelial lymphocytes were seen in control animals treated with S. thermophilus. S. thermophilus had no effect on gross pathology, histopathology or cytokine production in either colitic or control animals. We propose that S. thermophilus promotes maintenance of mucosal barrier function which reduces bacterial translocation, thereby reducing immune stimulation and associated inflammation. This allows mucosal healing, reducing gastrointestinal bleeding and weight loss. This could be studied as a locally-acting adjunct or alternative to current IBD treatments.
Purchase
Buy instant access (PDF download and unlimited online access):
Institutional Login
Log in with Open Athens, Shibboleth, or your institutional credentials
Personal login
Log in with your brill.com account
-
Agathou, C.L. and Beales, I.L., 2013. Factors associated with the use of probiotics in patients with inflammatory bowel disease. F1000 Research 2: 69.
'Factors associated with the use of probiotics in patients with inflammatory bowel disease ' () 2 F1000 Research : 69.
-
Alex, P., Zachos, N.C., Nguyen, T., Gonzales, L., Chen, T.E., Conklin, L.S., Centola, M. and Li, X., 2009. Distinct cytokine patterns identified from multiplex profiles of murine DSS and TNBS-induced colitis. Inflammatory Bowel Diseases 15: 341-352.
'Distinct cytokine patterns identified from multiplex profiles of murine DSS and TNBS-induced colitis ' () 15 Inflammatory Bowel Diseases : 341 -352.
-
Arnott, I.D., Kingstone, K. and Ghosh, S., 2000. Abnormal intestinal permeability predicts relapse in inactive Crohn disease. Scandinavian Journal of Gastroenterology 35: 1163-1169.
'Abnormal intestinal permeability predicts relapse in inactive Crohn disease ' () 35 Scandinavian Journal of Gastroenterology : 1163 -1169.
-
Bailey, J.R., Probert, C.S. and Cogan, T.A., 2011. Identification and characterisation of an iron-responsive candidate probiotic. PLoS ONE 6: e26507.
'Identification and characterisation of an iron-responsive candidate probiotic ' () 6 PLoS ONE : e26507.
-
Bruyneel, B., Vandewoestyne, M. and Verstraete, W., 1989. Lactic-acid bacteria – microorganisms able to grow in the absence of available iron and copper. Biotechnology Letters 11: 401-406.
'Lactic-acid bacteria – microorganisms able to grow in the absence of available iron and copper ' () 11 Biotechnology Letters : 401 -406.
-
Burisch, J., Jess, T., Martinato, M., Lakatos, P.L. and EpiCom, E., 2013. The burden of inflammatory bowel disease in Europe. Journal of Crohn’s and Colitis 7: 322-337.
'The burden of inflammatory bowel disease in Europe ' () 7 Journal of Crohn’s and Colitis : 322 -337.
-
Carlsson, A.H., Yakymenko, O., Olivier, I., Hakansson, F., Postma, E., Keita, A.V. and Soderholm, J.D., 2013. Faecalibacterium prausnitzii supernatant improves intestinal barrier function in mice DSS colitis. Scandinavian Journal of Gastroenterology 48: 1136-1144.
'Faecalibacterium prausnitzii supernatant improves intestinal barrier function in mice DSS colitis ' () 48 Scandinavian Journal of Gastroenterology : 1136 -1144.
-
Chassaing, B., Aitken, J.D., Malleshappa, M. and Vijay-Kumar, M., 2014. Dextran sulfate sodium (DSS)-induced colitis in mice. Current Protocols in Immunology 104: Unit 15-25.
'Dextran sulfate sodium (DSS)-induced colitis in mice ' () 104 Current Protocols in Immunology .
-
Chen, Y., Chou, K., Fuchs, E., Havran, W.L. and Boismenu, R., 2002. Protection of the intestinal mucosa by intraepithelial gamma delta T cells. Proceedings of the National Academy of Sciences of the USA 99: 14338-14343.
'Protection of the intestinal mucosa by intraepithelial gamma delta T cells ' () 99 Proceedings of the National Academy of Sciences of the USA : 14338 -14343.
-
Cogan, T.A., Thomas, A.O., Rees, L.E., Taylor, A.H., Jepson, M.A., Williams, P.H., Ketley, J. and Humphrey, T.J., 2007. Norepinephrine increases the pathogenic potential of Campylobacter jejuni. Gut 56: 1060-1065.
'Norepinephrine increases the pathogenic potential of Campylobacter jejuni ' () 56 Gut : 1060 -1065.
-
Daims, H., Bruhl, A., Amann, R., Schleifer, K.H. and Wagner, M., 1999. The domain-specific probe EUB338 is insufficient for the detection of all bacteria: development and evaluation of a more comprehensive probe set. Systematic and Applied Microbiology 22: 434-444.
'The domain-specific probe EUB338 is insufficient for the detection of all bacteria: development and evaluation of a more comprehensive probe set ' () 22 Systematic and Applied Microbiology : 434 -444.
-
Dalton, J.E., Cruickshank, S.M., Egan, C.E., Mears, R., Newton, D.J., Andrew, E.M., Lawrence, B., Howell, G., Else, K.J., Gubbels, M.J., Striepen, B., Smith, J.E., White, S.J. and Carding, S.R., 2006. Intraepithelial gammadelta+ lymphocytes maintain the integrity of intestinal epithelial tight junctions in response to infection. Gastroenterology 131: 818-829.
'Intraepithelial gammadelta+ lymphocytes maintain the integrity of intestinal epithelial tight junctions in response to infection ' () 131 Gastroenterology : 818 -829.
-
Duchmann, R., Kaiser, I., Hermann, E., Mayet, W., Ewe, K. and Meyer zum Buschenfelde, K.H., 1995. Tolerance exists towards resident intestinal flora but is broken in active inflammatory bowel disease (IBD). Clinical and Experimental Immunology 102: 448-455.
'Tolerance exists towards resident intestinal flora but is broken in active inflammatory bowel disease (IBD) ' () 102 Clinical and Experimental Immunology : 448 -455.
-
Food and Agriculture Organization of the United Nations/World Health Organization (FAO/WHO), 2001. Evaluation of health and nutritional properties of powder milk and live lactic acid bacteria. Available at: http://tinyurl.com/8bccc3r.
-
Freestone, P.P., Haigh, R.D., Williams, P.H. and Lyte, M., 1999. Stimulation of bacterial growth by heat-stable, norepinephrine-induced autoinducers. FEMS Microbiology Letters 172: 53-60.
'Stimulation of bacterial growth by heat-stable, norepinephrine-induced autoinducers ' () 172 FEMS Microbiology Letters : 53 -60.
-
Garcia-Hernandez, J., Moreno, Y., Amorocho, C.M. and Hernandez, M., 2012. A combination of direct viable count and fluorescence in situ hybridization for specific enumeration of viable Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus. Letters in Applied Microbiology 54: 247-254.
'A combination of direct viable count and fluorescence in situ hybridization for specific enumeration of viable Lactobacillus delbrueckii subsp ' () 54 Letters in Applied Microbiology : 247 -254.
-
Guy-Grand, D., DiSanto, J.P., Henchoz, P., Malassis-Seris, M. and Vassalli, P., 1998. Small bowel enteropathy: role of intraepithelial lymphocytes and of cytokines (IL-12, IFN-gamma, TNF) in the induction of epithelial cell death and renewal. European Journal of Immunology 28: 730-744.
'Small bowel enteropathy: role of intraepithelial lymphocytes and of cytokines (IL-12, IFN-gamma, TNF) in the induction of epithelial cell death and renewal ' () 28 European Journal of Immunology : 730 -744.
-
Heazlewood, C.K., Cook, M.C., Eri, R., Price, G.R., Tauro, S.B., Taupin, D., Thornton, D.J., Png, C.W., Crockford, T.L., Cornall, R.J., Adams, R., Kato, M., Nelms, K.A., Hong, N.A., Florin, T.H., Goodnow, C.C. and McGuckin, M.A., 2008. Aberrant mucin assembly in mice causes endoplasmic reticulum stress and spontaneous inflammation resembling ulcerative colitis. PLoS Medicine 5: e54.
'Aberrant mucin assembly in mice causes endoplasmic reticulum stress and spontaneous inflammation resembling ulcerative colitis ' () 5 PLoS Medicine : e54.
-
Heller, F., Florian, P., Bojarski, C., Richter, J., Christ, M., Hillenbrand, B., Mankertz, J., Gitter, A.H., Burgel, N., Fromm, M., Zeitz, M., Fuss, I., Strober, W. and Schulzke, J.D., 2005. Interleukin-13 is the key effector Th2 cytokine in ulcerative colitis that affects epithelial tight junctions, apoptosis, and cell restitution. Gastroenterology 129: 550-564.
'Interleukin-13 is the key effector Th2 cytokine in ulcerative colitis that affects epithelial tight junctions, apoptosis, and cell restitution ' () 129 Gastroenterology : 550 -564.
-
Imbert, M. and Blondeau, R., 1998. On the iron requirement of lactobacilli grown in chemically defined medium. Current Microbiology 37: 64-66.
'On the iron requirement of lactobacilli grown in chemically defined medium ' () 37 Current Microbiology : 64 -66.
-
Inagaki-Ohara, K., Chinen, T., Matsuzaki, G., Sasaki, A., Sakamoto, Y., Hiromatsu, K., Nakamura-Uchiyama, F., Nawa, Y. and Yoshimura, A., 2004. Mucosal T cells bearing TCRgammadelta play a protective role in intestinal inflammation. Journal of Immunology 173: 1390-1398.
'Mucosal T cells bearing TCRgammadelta play a protective role in intestinal inflammation ' () 173 Journal of Immunology : 1390 -1398.
-
Inagaki-Ohara, K., Dewi, F.N., Hisaeda, H., Smith, A.L., Jimi, F., Miyahira, M., Abdel-Aleem, A.S., Horii, Y. and Nawa, Y., 2006. Intestinal intraepithelial lymphocytes sustain the epithelial barrier function against Eimeria vermiformis infection. Infection and Immunity 74: 5292-5301.
'Intestinal intraepithelial lymphocytes sustain the epithelial barrier function against Eimeria vermiformis infection ' () 74 Infection and Immunity : 5292 -5301.
-
Inman, C.F., Rees, L.E., Barker, E., Haverson, K., Stokes, C.R. and Bailey, M., 2005. Validation of computer-assisted, pixel-based analysis of multiple-colour immunofluorescence histology. Journal of Immunological Methods 302: 156-167.
'Validation of computer-assisted, pixel-based analysis of multiple-colour immunofluorescence histology ' () 302 Journal of Immunological Methods : 156 -167.
-
Irvine, E.J. and Marshall, J.K., 2000. Increased intestinal permeability precedes the onset of Crohn’s disease in a subject with familial risk. Gastroenterology 119: 1740-1744.
'Increased intestinal permeability precedes the onset of Crohn’s disease in a subject with familial risk ' () 119 Gastroenterology : 1740 -1744.
-
Kortman, G.A., Boleij, A., Swinkels, D.W. and Tjalsma, H., 2012. Iron availability increases the pathogenic potential of Salmonella typhimurium and other enteric pathogens at the intestinal epithelial interface. PloS ONE 7: e29968.
'Iron availability increases the pathogenic potential of Salmonella typhimurium and other enteric pathogens at the intestinal epithelial interface ' () 7 PloS ONE : e29968.
-
Kruis, W., Fric, P., Pokrotnieks, J., Lukas, M., Fixa, B., Kascak, M., Kamm, M.A., Weismueller, J., Beglinger, C., Stolte, M., Wolff, C. and Schulze, J., 2004. Maintaining remission of ulcerative colitis with the probiotic Escherichia coli Nissle 1917 is as effective as with standard mesalazine. Gut 53: 1617-1623.
'Maintaining remission of ulcerative colitis with the probiotic Escherichia coli Nissle 1917 is as effective as with standard mesalazine ' () 53 Gut : 1617 -1623.
-
Kucharzik, T., Maaser, C., Lugering, A., Kagnoff, M., Mayer, L., Targan, S. and Domschke, W., 2006. Recent understanding of IBD pathogenesis: implications for future therapies. Inflammatory Bowel Diseases 12: 1068-1083.
'Recent understanding of IBD pathogenesis: implications for future therapies ' () 12 Inflammatory Bowel Diseases : 1068 -1083.
-
Luyer, M.D., Buurman, W.A., Hadfoune, M., Speelmans, G., Knol, J., Jacobs, J.A., Dejong, C.H., Vriesema, A.J. and Greve, J.W., 2005. Strain-specific effects of probiotics on gut barrier integrity following hemorrhagic shock. Infection and Immunity 73: 3686-3692.
'Strain-specific effects of probiotics on gut barrier integrity following hemorrhagic shock ' () 73 Infection and Immunity : 3686 -3692.
-
Madsen, K., Cornish, A., Soper, P., McKaigney, C., Jijon, H., Yachimec, C., Doyle, J., Jewell, L. and De Simone, C., 2001. Probiotic bacteria enhance murine and human intestinal epithelial barrier function. Gastroenterology 121: 580-591.
'Probiotic bacteria enhance murine and human intestinal epithelial barrier function ' () 121 Gastroenterology : 580 -591.
-
Matsumoto, S., Okabe, Y., Setoyama, H., Takayama, K., Ohtsuka, J., Funahashi, H., Imaoka, A., Okada, Y. and Umesaki, Y., 1998. Inflammatory bowel disease-like enteritis and caecitis in a senescence accelerated mouse P1/Yit strain. Gut 43: 71-78.
'Inflammatory bowel disease-like enteritis and caecitis in a senescence accelerated mouse P1/Yit strain ' () 43 Gut : 71 -78.
-
Miele, E., Pascarella, F., Giannetti, E., Quaglietta, L., Baldassano, R.N. and Staiano, A., 2009. Effect of a probiotic preparation (VSL#3) on induction and maintenance of remission in children with ulcerative colitis. American Journal of Gastroenterology 104: 437-443.
'Effect of a probiotic preparation (VSL#3) on induction and maintenance of remission in children with ulcerative colitis ' () 104 American Journal of Gastroenterology : 437 -443.
-
Ni, J., Chen, S.F. and Hollander, D., 1996. Effects of dextran sulphate sodium on intestinal epithelial cells and intestinal lymphocytes. Gut 39: 234-241.
'Effects of dextran sulphate sodium on intestinal epithelial cells and intestinal lymphocytes ' () 39 Gut : 234 -241.
-
Packey, C.D. and Sartor, R.B., 2009. Commensal bacteria, traditional and opportunistic pathogens, dysbiosis and bacterial killing in inflammatory bowel diseases. Current Opinion in Infectious Diseases 22: 292-301.
'Commensal bacteria, traditional and opportunistic pathogens, dysbiosis and bacterial killing in inflammatory bowel diseases ' () 22 Current Opinion in Infectious Diseases : 292 -301.
-
Pagnini, C., Saeed, R., Bamias, G., Arseneau, K.O., Pizarro, T.T. and Cominelli, F., 2010. Probiotics promote gut health through stimulation of epithelial innate immunity. Proceedings of the National Academy of Sciences of the USA 107: 454-459.
'Probiotics promote gut health through stimulation of epithelial innate immunity ' () 107 Proceedings of the National Academy of Sciences of the USA : 454 -459.
-
Pandey, A., Bringel, F. and Meyer, J.M., 1994. Iron requirement and search for siderophores in lactic-acid bacteria. Applied Microbiology and Biotechnology 40: 735-739.
'Iron requirement and search for siderophores in lactic-acid bacteria ' () 40 Applied Microbiology and Biotechnology : 735 -739.
-
Pastorelli, L., De Salvo, C., Mercado, J.R., Vecchi, M. and Pizarro, T.T., 2013. Central role of the gut epithelial barrier in the pathogenesis of chronic intestinal inflammation: lessons learned from animal models and human genetics. Frontiers in Immunology 4: 280.
'Central role of the gut epithelial barrier in the pathogenesis of chronic intestinal inflammation: lessons learned from animal models and human genetics ' () 4 Frontiers in Immunology : 280.
-
Roselli, M., Finamore, A., Nuccitelli, S., Carnevali, P., Brigidi, P., Vitali, B., Nobili, F., Rami, R., Garaguso, I. and Mengheri, E., 2009. Prevention of TNBS-induced colitis by different Lactobacillus and Bifidobacterium strains is associated with an expansion of gammadeltaT and regulatory T cells of intestinal intraepithelial lymphocytes. Inflammatory Bowel Diseases 15: 1526-1536.
'Prevention of TNBS-induced colitis by different Lactobacillus and Bifidobacterium strains is associated with an expansion of gammadeltaT and regulatory T cells of intestinal intraepithelial lymphocytes ' () 15 Inflammatory Bowel Diseases : 1526 -1536.
-
Sanders, M.E., Guarner, F., Guerrant, R., Holt, P.R., Quigley, E.M., Sartor, R.B., Sherman, P.M. and Mayer, E.A., 2013. An update on the use and investigation of probiotics in health and disease. Gut 62: 787-796.
'An update on the use and investigation of probiotics in health and disease ' () 62 Gut : 787 -796.
-
Shavrov, A., Kharitonova, A.Y., Davis, E.M., Claggett, B., Morozov, D.A., Brown, D.K., Shavrov, A.A. and Liu, J.J., 2015. A pilot study of the predictive value of barrier dysfunction for relapse in pediatric inflammatory bowel disease. Journal of Pediatric Gastroenterology and Nutrition 62: 873-878.
'A pilot study of the predictive value of barrier dysfunction for relapse in pediatric inflammatory bowel disease ' () 62 Journal of Pediatric Gastroenterology and Nutrition : 873 -878.
-
Sood, A., Midha, V., Makharia, G.K., Ahuja, V., Singal, D., Goswami, P. and Tandon, R.K., 2009. The probiotic preparation, VSL#3 induces remission in patients with mild-to-moderately active ulcerative colitis. Clinical Gastroenterology and Hepatology 7: 1202-1209, 1209 e1201.
'The probiotic preparation, VSL#3 induces remission in patients with mild-to-moderately active ulcerative colitis ' () 7 Clinical Gastroenterology and Hepatology : 1202 -1209.
-
Stein, J., Bager, P., Befrits, R., Gasche, C., Gudehus, M., Lerebours, E., Magro, F., Mearin, F., Mitchell, D., Oldenburg, B. and Danese, S., 2013. Anaemia management in patients with inflammatory bowel disease: routine practice across nine European countries. European Journal of Gastroenterology and Hepatology 25: 1456-1463.
'Anaemia management in patients with inflammatory bowel disease: routine practice across nine European countries ' () 25 European Journal of Gastroenterology and Hepatology : 1456 -1463.
-
Sturniolo, G.C., Mestriner, C., Lecis, P.E., D’Odorico, A., Venturi, C., Irato, P., Cecchetto, A., Tropea, A., Longo, G. and D’Inca, R., 1998. Altered plasma and mucosal concentrations of trace elements and antioxidants in active ulcerative colitis. Scandinavian Journal of Gastroenterology 33: 644-649.
'Altered plasma and mucosal concentrations of trace elements and antioxidants in active ulcerative colitis ' () 33 Scandinavian Journal of Gastroenterology : 644 -649.
-
Sugawara, K., Olson, T.S., Moskaluk, C.A., Stevens, B.K., Hoang, S., Kozaiwa, K., Cominelli, F., Ley, K.F. and McDuffie, M., 2005. Linkage to peroxisome proliferator-activated receptor-gamma in SAMP1/YitFc mice and in human Crohn’s disease. Gastroenterology 128: 351-360.
'Linkage to peroxisome proliferator-activated receptor-gamma in SAMP1/YitFc mice and in human Crohn’s disease ' () 128 Gastroenterology : 351 -360.
-
Sund, M., Xu, L.L., Rahman, A., Qian, B.F., Hammarstrom, M.L. and Danielsson, A., 2005. Reduced susceptibility to dextran sulphate sodium-induced colitis in the interleukin-2 heterozygous (IL-2) mouse. Immunology 114: 554-564.
'Reduced susceptibility to dextran sulphate sodium-induced colitis in the interleukin-2 heterozygous (IL-2) mouse ' () 114 Immunology : 554 -564.
-
Vivinus-Nebot, M., Frin-Mathy, G., Bzioueche, H., Dainese, R., Bernard, G., Anty, R., Filippi, J., Saint-Paul, M.C., Tulic, M.K., Verhasselt, V., Hebuterne, X. and Piche, T., 2014. Functional bowel symptoms in quiescent inflammatory bowel diseases: role of epithelial barrier disruption and low-grade inflammation. Gut 63: 744-752.
'Functional bowel symptoms in quiescent inflammatory bowel diseases: role of epithelial barrier disruption and low-grade inflammation ' () 63 Gut : 744 -752.
-
Wooldridge, K.G. and Williams, P.H., 1993. Iron uptake mechanisms of pathogenic bacteria. FEMS Microbiology Reviews 12: 325-348.
'Iron uptake mechanisms of pathogenic bacteria ' () 12 FEMS Microbiology Reviews : 325 -348.
-
Wyatt, J., Vogelsang, H., Hubl, W., Waldhoer, T. and Lochs, H., 1993. Intestinal permeability and the prediction of relapse in Crohn’s disease. Lancet 341: 1437-1439.
'Intestinal permeability and the prediction of relapse in Crohn’s disease ' () 341 Lancet : 1437 -1439.
-
Xavier, R.J. and Podolsky, D.K., 2007. Unravelling the pathogenesis of inflammatory bowel disease. Nature 448: 427-434.
'Unravelling the pathogenesis of inflammatory bowel disease ' () 448 Nature : 427 -434.
-
Zakostelska, Z., Kverka, M., Klimesova, K., Rossmann, P., Mrazek, J., Kopecny, J., Hornova, M., Srutkova, D., Hudcovic, T., Ridl, J. and Tlaskalova-Hogenova, H., 2011. Lysate of probiotic Lactobacillus casei DN-114 001 ameliorates colitis by strengthening the gut barrier function and changing the gut microenvironment. PloS ONE 6: e27961.
'Lysate of probiotic Lactobacillus casei DN-114 001 ameliorates colitis by strengthening the gut barrier function and changing the gut microenvironment ' () 6 PloS ONE : e27961.
-
Zeissig, S., Burgel, N., Gunzel, D., Richter, J., Mankertz, J., Wahnschaffe, U., Kroesen, A.J., Zeitz, M., Fromm, M. and Schulzke, J.D., 2007. Changes in expression and distribution of claudin 2, 5 and 8 lead to discontinuous tight junctions and barrier dysfunction in active Crohn’s disease. Gut 56: 61-72.
'Changes in expression and distribution of claudin 2, 5 and 8 lead to discontinuous tight junctions and barrier dysfunction in active Crohn’s disease ' () 56 Gut : 61 -72.
-
Zeng, J., Li, Y.Q., Zuo, X.L., Zhen, Y.B., Yang, J. and Liu, C.H., 2008. Clinical trial: effect of active lactic acid bacteria on mucosal barrier function in patients with diarrhoea-predominant irritable bowel syndrome. Alimentary Pharmacology and Therapeutics 28: 994-1002.
'Clinical trial: effect of active lactic acid bacteria on mucosal barrier function in patients with diarrhoea-predominant irritable bowel syndrome ' () 28 Alimentary Pharmacology and Therapeutics : 994 -1002.
| All Time | Past 365 days | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 582 | 208 | 46 |
| Full Text Views | 40 | 7 | 1 |
| PDF Views & Downloads | 43 | 16 | 3 |
Streptococcus thermophilus NCIMB 41856 ameliorates signs of colitis in an animal model of inflammatory bowel disease
In: Beneficial MicrobesSearch for other papers by J.R. Bailey in
Current site
Google Scholar
PubMed
Search for other papers by V. Vince in
Current site
Google Scholar
PubMed
Search for other papers by N.A. Williams in
Current site
Google Scholar
PubMed
Search for other papers by T.A. Cogan in
Current site
Google Scholar
PubMed
Treatment of inflammatory bowel disease (IBD) is mainly based on suppression of symptoms, often with numerous side effects. Trials of probiotics in IBD have frequently produced disappointing results. The majority of probiotics are unusual, since they do not require iron for growth, unlike many bacteria resident in the intestine. The IBD intestine is iron-rich due to bleeding and use of oral iron supplements; conventional probiotics would be rapidly outcompeted. We have evaluated an iron-responsive Streptococcus thermophilus strain for its potential to reduce signs of colitis. Efficacy of S. thermophilus was evaluated in the dextran sodium sulphate mouse model of colitis. Treated animals were given 1×108 cfu S. thermophilus per day and clinical observations were taken daily. At termination, gross and histopathological signs of disease, cellular infiltration, location of bacteria, and cytokine expression in the intestine were determined. S. thermophilus delayed onset of colitis and reduced clinical signs of disease, including bodyweight loss and gastrointestinal bleeding. It reduced bacterial translocation into the colonic tissue. Increased numbers of CD8+ intraepithelial lymphocytes were seen in control animals treated with S. thermophilus. S. thermophilus had no effect on gross pathology, histopathology or cytokine production in either colitic or control animals. We propose that S. thermophilus promotes maintenance of mucosal barrier function which reduces bacterial translocation, thereby reducing immune stimulation and associated inflammation. This allows mucosal healing, reducing gastrointestinal bleeding and weight loss. This could be studied as a locally-acting adjunct or alternative to current IBD treatments.
| All Time | Past 365 days | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 582 | 208 | 46 |
| Full Text Views | 40 | 7 | 1 |
| PDF Views & Downloads | 43 | 16 | 3 |
