Establishment of Lactobacillus plantarum strain in honey bee digestive tract monitored using gfp fluorescence
äºBeneficial MicrobesSearch for other papers by P. Javorský in
Current site
Google Scholar
Search for other papers by L. Kolesár Fecskeová in
Current site
Google Scholar
Search for other papers by L. Hrehová in
Current site
Google Scholar
Search for other papers by R. Sabo in
Current site
Google Scholar
Search for other papers by J. Legáth in
Current site
Google Scholar
Institute of Biology and Ecology, Faculty of Science, Pavol Jozef Šafárik University, Šrobárová 2, 041 80 Košice, Slovakia.
Search for other papers by P. Pristas in
Current site
Google Scholar
Lactic acid bacteria are symbiotic bacteria that naturally reside in the gastrointestinal tract of honey bees. They serve a multitude of functions and are considered beneficial and completely harmless. In our experiments Lactobacillus plantarum strain B35, isolated from honey bee digestive tract, was modified using pAD43-25 plasmid carrying a functional GFP gene sequence (gfpmut3a) and used as a model for monitoring and optimisation of the mode of application. The establishment of this strain in honey bee digestive tract was monitored using GFP fluorescence. Three different modes of oral application of this strain were tested: water suspension of lyophilised bacteria, aerosol application of these bacteria and consumption of sugar honey paste containing the lyophilised lactobacilli. Two days after administration the L. plantarum B35-gfp was present throughout the honey bee digestive tract with 104-105 cfu/bee with highest count observed for aerosol application.
Purchase
Buy instant access (PDF download and unlimited online access):
Institutional Login
Log in with Open Athens, Shibboleth, or your institutional credentials
Personal login
Log in with your brill.com account
-
Audisio, M.C., Torres, M.J., Sabate, D.G, Ibarguren, C. and Apella, M., 2011. Properties of different lactic bacteria isolated from Apis millifera L. bee-gut. Microbial Research 166: 1-13.
'Properties of different lactic bacteria isolated from Apis millifera L. bee-gut ' () 166 Microbial Research : 1 -13.
-
Asama, T., Arima, T.-H., Gomi, T., Keishi, T., Tani, H., Kimura, Y., Tatefuji, T. and Hashimoto, K., 2015. Lactobacillus kunkeei YB38 from honeybee products enhances IgA production in healthy adults. Journal of Applied Microbiology 119: 818-826.
'Lactobacillus kunkeei YB38 from honeybee products enhances IgA production in healthy adults ' () 119 Journal of Applied Microbiology : 818 -826.
-
Berner, D. and Viernstein, V., 2006. Effect of protective agents on the viability of Lactococcus lactis subjected to freeze-thawing and freeze-drying. Scientia Pharmaceutica 74: 137-149.
'Effect of protective agents on the viability of Lactococcus lactis subjected to freeze-thawing and freeze-drying ' () 74 Scientia Pharmaceutica : 137 -149.
-
Canganella, F. and Balsamo, R., 2008. Isolation and selection of probiotic microorganisms with antagonistic activities against Paenibacillus larvae and Paenibacillus alvei. In: Proceedings of the International Probiotic Conference, Probiotics for the 3rd Millennium, 4-7 June 2008, High Tatras, Slovakia, p. 28.
Isolation and selection of probiotic microorganisms with antagonistic activities against Paenibacillus larvae and Paenibacillus alvei 28
-
Evans, J.D. and Lopez, D.L., 2004. Bacterial probiotics induce an immune response in the honey bee (Hymenoptera: Apidae). Journal of Economic Entomology 97: 752-756.
'Bacterial probiotics induce an immune response in the honey bee (Hymenoptera: Apidae) ' () 97 Journal of Economic Entomology : 752 -756.
-
Forsgren, E., Olofsson, T.C., V´asquez, A. and Fries, I., 2010. Novel lactic acid bacteria inhibiting Paenibacillus larvae in honey bee larvae. Apidologie 41: 99-108.
'Novel lactic acid bacteria inhibiting Paenibacillus larvae in honey bee larvae ' () 41 Apidologie : 99 -108.
-
Fuller, R., 1989. Probiotics in man and animals. Journal of Applied Bacteriology 66: 365-378.
'Probiotics in man and animals ' () 66 Journal of Applied Bacteriology : 365 -378.
-
Geoffroy, M.C., Guyard, C., Quatannens, B., Pavan, S., Lange, M. and Mercenier, A., 2000. Use of green fluorescent protein to tag lactic acid bacterium strains under development as live vaccine vectors. Applied and Environmental Microbiology 66: 383-391.
'Use of green fluorescent protein to tag lactic acid bacterium strains under development as live vaccine vectors ' () 66 Applied and Environmental Microbiology : 383 -391.
-
Guinane, C.M., Kent, R.M., Norberg, S., Hill, C., Fitzgerald, G.F., Stanton, C. and Ross, R.P., 2011. Host specific diversity in Lactobacillus johnsonii as evidenced by a major chromosomal inversion and phage resistance mechanisms. PLoS One 6: e18740.
'Host specific diversity in Lactobacillus johnsonii as evidenced by a major chromosomal inversion and phage resistance mechanisms ' () 6 PLoS One : e18740.
-
Han, X., Wang, L., Li, W., Li, B., Yang, Y., Yan, H., Qu, L. and Chen, Y., 2015. Use of green fluorescent protein to monitor Lactobacillus plantarum in the gastrointestinal tract of goats. Brazilian Journal of Microbiology 46: 849-854.
'Use of green fluorescent protein to monitor Lactobacillus plantarum in the gastrointestinal tract of goats ' () 46 Brazilian Journal of Microbiology : 849 -854.
-
Hroncova, Z., Havlik, J., Killer, J., Doskocil, I., Tyl, J., Kamler, M., Titera, D., Hakl, J., Mrazek, J., Bunesova, V. and Rada, V., 2015. Variation in honey bee gut microbial diversity affected by ontogenetic stage, age and geographic location. PLoS ONE 10: e0118707.
'Variation in honey bee gut microbial diversity affected by ontogenetic stage, age and geographic location ' () 10 PLoS ONE : e0118707.
-
Kacaniova, M., Chlebo, R., Kopernicky, M. and Trakovicka, A., 2004. Microflora of the honeybee gastrointestinal tract. Folia Microbiologica 49: 169-171.
'Microflora of the honeybee gastrointestinal tract ' () 49 Folia Microbiologica : 169 -171.
-
Killer, J., KopeÄný, J., Mrázek, J., Rada, V., Dubná, S. and Marounek, M., 2010. Bifidobacteria in the digestive tract of bumblebees. Anaerobe 16: 165-170.
'Bifidobacteria in the digestive tract of bumblebees ' () 16 Anaerobe : 165 -170.
-
Máchová, M., Rada, V., Huk, J. and Smékal, F., 1997. Development of probiotics for bees. Apiacta 22: 99-111.
'Development of probiotics for bees ' () 22 Apiacta : 99 -111.
-
Mitarai, N., Jensen, M.H. and Semsey, S., 2015. Coupled positive and negative feedbacks produce diverse gene expression patterns in colonies. mBio 6: e00059-15.
'Coupled positive and negative feedbacks produce diverse gene expression patterns in colonies ' () 6 mBio : e00059 -15.
-
Miyamoto-Shinohara, Y., Imaizumi, T., Sukenobe, J., Marakami, Y., Kawamura, S. and Komatsu, Y., 2000. Survival rate of microbes after freeze-drying and long-term storage. Cryobiology 41: 251-255.
'Survival rate of microbes after freeze-drying and long-term storage ' () 41 Cryobiology : 251 -255.
-
Mohr, K.I. and Tebbe, C.C., 2006. Diversity and phylotype consistency of bacteria in the guts of three bee species (Apoidea) at an oilseed rape field. Environmental Microbiology 8: 258-272.
'Diversity and phylotype consistency of bacteria in the guts of three bee species (Apoidea) at an oilseed rape field ' () 8 Environmental Microbiology : 258 -272.
-
Mrázek, J., Å trosová, L., Fliegerová, K., Kott, T. and KopeÄný, J., 2008. Diversity of insect intestinal microflora. Folia Microbiologica 53: 229-233.
'Diversity of insect intestinal microflora ' () 53 Folia Microbiologica : 229 -233.
-
MudroÅová, D., ToporÄák, J., Nemcová, R., GancarÄÃková, S., HajduÄková, V. and Rumanovská, K., 2011. Lactobacillus sp. as a potential probiotic for the prevention of Paenibacillus larvae infection in honey bees. Journal of Apicultural Research 50: 323-324.
'Lactobacillus sp. as a potential probiotic for the prevention of Paenibacillus larvae infection in honey bees ' () 50 Journal of Apicultural Research : 323 -324.
-
Organization for Economic Cooperation and Development (OECD), 1998. Guidance for the testing of chemicals. Honeybees Acute Oral Toxicity test. Test Guidelines 213. Available at: http://tinyurl.com/qgf7ot6.
-
Poppinga, L. and Genersch, E., 2011. Heterologous expression of green fluorescent protein in Paenibacillus larvae, the causative agent of American Foulbrood of honey bees. Journal of Applied Microbiology 112: 430-439.
'Heterologous expression of green fluorescent protein in Paenibacillus larvae, the causative agent of American Foulbrood of honey bees ' () 112 Journal of Applied Microbiology : 430 -439.
-
Phumkhachorn, P., Rattanachaikunsopon, P. and Khunsook, S., 2007. Use of the gfp gene in monitoring bacteriocin-producing Lactobacillus plantarum N014, a potential starter culture in nham fermentation. Journal of Food Protection 70: 419-424.
'Use of the gfp gene in monitoring bacteriocin-producing Lactobacillus plantarum N014, a potential starter culture in nham fermentation ' () 70 Journal of Food Protection : 419 -424.
-
Rada, V., Máchová, M., Huk, J., Marounek, M. and Dusková, D., 1997. Microflora in the honeybee digestive tract: counts, characteristics and sensitivity to veterinary drugs. Apidologie 28: 357-365.
'Microflora in the honeybee digestive tract: counts, characteristics and sensitivity to veterinary drugs ' () 28 Apidologie : 357 -365.
-
Rada, V., HavlÃk, J. and Flesar, J., 2009. Biologically active compounds in honey bee nutrition (in Czech). Výzkumný ústav živoÄÃÅ¡ne výroby, Prague, Czech Republic, 52 pp.
'Biologically active compounds in honey bee nutrition (in Czech) ', () 52.
-
Rangberg, A., Mathiesen, G., Amdam, G.V. and Diep, D.B., 2015. The paratransgenic potential of Lactobacillus kunkeei in the honey bee Apis mellifera. Beneficial Microbes 6: 513-523.
'The paratransgenic potential of Lactobacillus kunkeei in the honey bee Apis mellifera ' () 6 Beneficial Microbes : 513 -523.
-
Tajabadi, N., Mardan, M., Saari, N., Mustafa, S., Bahreini, R., Yazid, M. and Manap, A., 2013. Identification of Lactobacillus plantarum, Lactobacillus pentosus and Lactobacillus fermentum from honey stomach of honeybee. Brazilian Journal of Microbiology 44: 717-722.
'Identification of Lactobacillus plantarum, Lactobacillus pentosus and Lactobacillus fermentum from honey stomach of honeybee ' () 44 Brazilian Journal of Microbiology : 717 -722.
-
Thompson, K. and Collins, M.A., 1996. Improvement in electroporation efficiency for Lactobacillus plantarum by the inclusion of high concentrations of glycine in the growth medium. Journal of Microbiological Methods 26: 73-79.
'Improvement in electroporation efficiency for Lactobacillus plantarum by the inclusion of high concentrations of glycine in the growth medium ' () 26 Journal of Microbiological Methods : 73 -79.
-
Tsien, R.Y., 1998. The green fluorescent protein. Annual Review of Biochemistry 67: 509-544.
'The green fluorescent protein ' () 67 Annual Review of Biochemistry : 509 -544.
-
Vanikova, S., Noskova, A., Pristas, Judova, J. and Javorsky, P., 2015. Heterotrophic bacteria associated with Varroa destructor mite. Apidologie 46: 369-379.
'Heterotrophic bacteria associated with Varroa destructor mite ' () 46 Apidologie : 369 -379.
-
Yu, Q.H., Dong, S.M., Zhu, W.Y. and Yang, Q., 2007. Use of green fluorescent protein to monitor Lactobacillus in the gastro-intestinal tract of chicken. FEMS Microbiology Letters 275: 207-213.
'Use of green fluorescent protein to monitor Lactobacillus in the gastro-intestinal tract of chicken ' () 275 FEMS Microbiology Letters : 207 -213.
| å ¨é¨æé´ | è¿å»ä¸å¹´ | è¿å»30天 | |
|---|---|---|---|
| æè¦æµè§æ¬¡æ° | 382 | 111 | 17 |
| å ¨ææµè§æ¬¡æ° | 14 | 0 | 0 |
| PDFä¸è½½æ¬¡æ° | 23 | 0 | 0 |
Establishment of Lactobacillus plantarum strain in honey bee digestive tract monitored using gfp fluorescence
äºBeneficial MicrobesSearch for other papers by P. Javorský in
Current site
Google Scholar
PubMed
Search for other papers by L. Kolesár Fecskeová in
Current site
Google Scholar
PubMed
Search for other papers by L. Hrehová in
Current site
Google Scholar
PubMed
Search for other papers by R. Sabo in
Current site
Google Scholar
PubMed
Search for other papers by J. Legáth in
Current site
Google Scholar
PubMed
Institute of Biology and Ecology, Faculty of Science, Pavol Jozef Šafárik University, Šrobárová 2, 041 80 Košice, Slovakia.
Search for other papers by P. Pristas in
Current site
Google Scholar
PubMed
Lactic acid bacteria are symbiotic bacteria that naturally reside in the gastrointestinal tract of honey bees. They serve a multitude of functions and are considered beneficial and completely harmless. In our experiments Lactobacillus plantarum strain B35, isolated from honey bee digestive tract, was modified using pAD43-25 plasmid carrying a functional GFP gene sequence (gfpmut3a) and used as a model for monitoring and optimisation of the mode of application. The establishment of this strain in honey bee digestive tract was monitored using GFP fluorescence. Three different modes of oral application of this strain were tested: water suspension of lyophilised bacteria, aerosol application of these bacteria and consumption of sugar honey paste containing the lyophilised lactobacilli. Two days after administration the L. plantarum B35-gfp was present throughout the honey bee digestive tract with 104-105 cfu/bee with highest count observed for aerosol application.
| å ¨é¨æé´ | è¿å»ä¸å¹´ | è¿å»30天 | |
|---|---|---|---|
| æè¦æµè§æ¬¡æ° | 382 | 111 | 17 |
| å ¨ææµè§æ¬¡æ° | 14 | 0 | 0 |
| PDFä¸è½½æ¬¡æ° | 23 | 0 | 0 |
