Probiotic administration improves sperm quality in asthenozoospermic human donors
äºBeneficial MicrobesINDEGSAL, University of León, Campus de Vegazana, 24071 León, Spain.
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INDEGSAL, University of León, Campus de Vegazana, 24071 León, Spain.
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INDEGSAL, University of León, Campus de Vegazana, 24071 León, Spain.
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The objective of this study is to analyse the effect of the ingestion of two selected antioxidant probiotics strains (Lactobacillus rhamnosus CECT8361 and Bifidobacterium longum CECT7347) on sperm quality parameters in asthenozoospermic males after three and six weeks of administration. Nine asthenozoospermic men without any medical treatment under similar diet conditions participated in the study. The quality of individual sperm samples was evaluated before (previous to ingestion), during (after 3 and 6 weeks of ingestion) and after probiotic administration (3 and 6 weeks after finishing the treatment). Sperm motility was evaluated by computer-assisted sperm analysis system, DNA fragmentation by sperm chromatin structure assay, cell viability by flow cytometry and measurement of intracellular H2O2 (reactive oxygen species; ROS) by flow cytometry using dichloro-dihydrofluorescein diacetate. Sperm motility was drastically improved after the treatment (approximately 6 fold change), DNA fragmentation was statistically reduced after probiotic administration from (approximately 1.2 fold change) and intracellular H2O2 level was decreased (approximately 3.5 fold change). Cell viability was not affected by the treatment. The significant improvement in sperm motility and the decrease in DNA fragmentation reported in this study provide preliminary evidence that probiotics could be administrated to improve motility and decrease DNA fragmentation and ROS levels in asthenozoospermic human males.
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| å ¨é¨æé´ | è¿å»ä¸å¹´ | è¿å»30天 | |
|---|---|---|---|
| æè¦æµè§æ¬¡æ° | 2099 | 1071 | 48 |
| å ¨ææµè§æ¬¡æ° | 43 | 19 | 0 |
| PDFä¸è½½æ¬¡æ° | 79 | 49 | 0 |
Probiotic administration improves sperm quality in asthenozoospermic human donors
äºBeneficial MicrobesINDEGSAL, University of León, Campus de Vegazana, 24071 León, Spain.
Search for other papers by D.G. Valcarce in
Current site
Google Scholar
PubMed
Search for other papers by S. Genovés in
Current site
Google Scholar
PubMed
INDEGSAL, University of León, Campus de Vegazana, 24071 León, Spain.
Search for other papers by M.F. Riesco in
Current site
Google Scholar
PubMed
Search for other papers by P. Martorell in
Current site
Google Scholar
PubMed
INDEGSAL, University of León, Campus de Vegazana, 24071 León, Spain.
Search for other papers by M.P. Herráez in
Current site
Google Scholar
PubMed
Search for other papers by D. Ramón in
Current site
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PubMed
INDEGSAL, University of León, Campus de Vegazana, 24071 León, Spain.
Search for other papers by V. Robles in
Current site
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PubMed
The objective of this study is to analyse the effect of the ingestion of two selected antioxidant probiotics strains (Lactobacillus rhamnosus CECT8361 and Bifidobacterium longum CECT7347) on sperm quality parameters in asthenozoospermic males after three and six weeks of administration. Nine asthenozoospermic men without any medical treatment under similar diet conditions participated in the study. The quality of individual sperm samples was evaluated before (previous to ingestion), during (after 3 and 6 weeks of ingestion) and after probiotic administration (3 and 6 weeks after finishing the treatment). Sperm motility was evaluated by computer-assisted sperm analysis system, DNA fragmentation by sperm chromatin structure assay, cell viability by flow cytometry and measurement of intracellular H2O2 (reactive oxygen species; ROS) by flow cytometry using dichloro-dihydrofluorescein diacetate. Sperm motility was drastically improved after the treatment (approximately 6 fold change), DNA fragmentation was statistically reduced after probiotic administration from (approximately 1.2 fold change) and intracellular H2O2 level was decreased (approximately 3.5 fold change). Cell viability was not affected by the treatment. The significant improvement in sperm motility and the decrease in DNA fragmentation reported in this study provide preliminary evidence that probiotics could be administrated to improve motility and decrease DNA fragmentation and ROS levels in asthenozoospermic human males.
| å ¨é¨æé´ | è¿å»ä¸å¹´ | è¿å»30天 | |
|---|---|---|---|
| æè¦æµè§æ¬¡æ° | 2099 | 1071 | 48 |
| å ¨ææµè§æ¬¡æ° | 43 | 19 | 0 |
| PDFä¸è½½æ¬¡æ° | 79 | 49 | 0 |
