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Inflammation-related pro-apoptotic activity of exopolysaccharides isolated from Lactococcus lactis subsp. lactis

于Beneficial Microbes
著者:
Z. Wu Department of Food Science and Nutrition, Ginling College, Nanjing Normal University, Nanjing 210097, Jiangsu, China P.R.
Key Laboratory of Animal Protein Deep Processing Technology of Zhejiang Province, Marine Science School, Ningbo University, Ningbo 315211, Zhejiang, China P.R.

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G. Wang Department of Landscape Engineering, Heze University, Heze 274051, Shandong, China P.R.

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D. Pan Department of Food Science and Nutrition, Ginling College, Nanjing Normal University, Nanjing 210097, Jiangsu, China P.R.
Key Laboratory of Animal Protein Deep Processing Technology of Zhejiang Province, Marine Science School, Ningbo University, Ningbo 315211, Zhejiang, China P.R.

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Y. Guo Department of Food Science and Nutrition, Ginling College, Nanjing Normal University, Nanjing 210097, Jiangsu, China P.R.

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X. Zeng Key Laboratory of Animal Protein Deep Processing Technology of Zhejiang Province, Marine Science School, Ningbo University, Ningbo 315211, Zhejiang, China P.R.

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Y. Sun Key Laboratory of Animal Protein Deep Processing Technology of Zhejiang Province, Marine Science School, Ningbo University, Ningbo 315211, Zhejiang, China P.R.

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J. Cao Key Laboratory of Animal Protein Deep Processing Technology of Zhejiang Province, Marine Science School, Ningbo University, Ningbo 315211, Zhejiang, China P.R.

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Exopolysaccharides (EPS) have attracted attention recently for possible use in suppressing early stage breast cancer. In this study, a mannan EPS produced by Lactococcus lactis subsp. lactis was found to affect the production of inflammatory cytokines. EPS (300 μg/ml) can significantly enhance tumour necrosis factor alpha and inducible NO synthase release in MCF-7 cells compared to control cells in a concentration-dependent manner. Also the intracellular calcium level was found to increase with the concentration of EPS. After EPS-treatment, a significant reduction in mitochondrial potential was observed, as was nuclear condensation and cell shrinkage. These results may be helpful in further understanding the anti-tumour properties of lactic acid bacteria.

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