The interaction between the enteric microbiota and intestinal cells often involves signal molecules that affect both microbial behaviour and host responses. Examples of such signal molecules are the molecules secreted by bacteria that induce quorum sensing mechanisms in the producing microorganism and signal transduction pathways in the host cells. The pentapeptide competence and sporulation factor (CSF) of Bacillus subtilis is a well characterized quorum sensing factor that controls competence and spore formation in the producing bacterium and induces cytoprotective heat shock proteins in intestinal epithelial cells. We analysed several Bacillus strains isolated from human ileal biopsies of healthy volunteers and observed that some of them were unable to produce CSF but still able to act in a CSF-like fashion on model intestinal epithelial cells. One of those strains belonging to the Bacillus megaterium species secreted at least two factors with effects on intestinal HT29 cells: a peptide smaller than 3 kDa able to induce heat shock protein 27 (hsp27) and p38-MAPK, and a larger molecule able to induce protein kinase B (PKB/Akt) with a pro-proliferative effect.
Purchase
Buy instant access (PDF download and unlimited online access):
Institutional Login
Log in with Open Athens, Shibboleth, or your institutional credentials
Personal login
Log in with your brill.com account
Baccigalupi, L., Ricca, E. and Ghelardi, E., 2015. Non-LAB probiotics: spore formers. In: Venema, K. and Do Carmo, A.P. (eds.) Probiotics and prebiotics: current research and future trends. Caister Academic Press, Poole, UK, pp. 93-103.
'Non-LAB probiotics: spore formers ', in Probiotics and prebiotics: current research and future trends , () 93 -103.
Bassler, B.L. and Losick, R., 2006. Bacterially speaking. Cell 125: 237-246.
'Bacterially speaking ' () 125 Cell : 237 -246.
Bongiorni, C., Ishikawa, S., Stephenson, S., Ogasawara, N. and Perego, M., 2005. Synergistic regulation of competence development in Bacillus subtilis by two Rap-Phr systems. Journal of Bacteriology 187: 4353-4361.
'Synergistic regulation of competence development in Bacillus subtilis by two Rap-Phr systems ' () 187 Journal of Bacteriology : 4353 -4361.
Camilli, A. and Bassler, B.L., 2006. Bacterial small-molecule signaling pathways. Science 311: 1113-1116.
'Bacterial small-molecule signaling pathways ' () 311 Science : 1113 -1116.
Chevreux, B., Wetter, T, and Suhai, S., 1999. Genome sequence assembly using trace signals and additional sequence information, In: German Conference on Bioinformatics, p 45-56.
Genome sequence assembly using trace signals and additional sequence information 45 56
Cutting, S.M., 2011. Bacillus probiotics. Food Microbiology 28: 214-220.
'Bacillus probiotics ' () 28 Food Microbiology : 214 -220.
Cutting S. and Vander, Horn, P.B., 1990. Genetic analysis. In: Harwood, C. and Cutting, S. (eds.) Molecular biological methods for Bacillus. John Wiley and Sons, Chichester, UK, pp. 27-74.
'Genetic analysis ', in Molecular biological methods for Bacillus , () 27 -74.
Cutting, S.M., Hong, H.A., Baccigalupi, L. and Ricca, E., 2009. Oral vaccine delivery by recombinant spore probiotics. International Review of Immunology 28: 487-505.
'Oral vaccine delivery by recombinant spore probiotics ' () 28 International Review of Immunology : 487 -505.
DâArienzo, R., Maurano, F., Mazzarella, G., Luongo, D., Stefanile, R., Ricca, E. and Rossi, M., 2006. Bacillus subtilis spores reduce susceptibility to Citrobacter rodentium-mediated enteropathy in a mouse model. Research in Microbiology 157: 891-897.
'Bacillus subtilis spores reduce susceptibility to Citrobacter rodentium-mediated enteropathy in a mouse model ' () 157 Research in Microbiology : 891 -897.
Di Luccia, B., Manzo, N., Baccigalupi, L., Calabrò, V., Crescenzi, E., Ricca, E. and Pollice, A., 2013. Lactobacillus gasseri SF1183 affects intestinal epithelial cell survival and growth. PLoS One 8: e69102.
'Lactobacillus gasseri SF1183 affects intestinal epithelial cell survival and growth ' () 8 PLoS One : e69102.
Diehl, J.A., Cheng, M., Roussel, M.F. and Sherr, C.J., 1998. Glycogen synthase kinase-3beta regulates cyclin D1 proteolysis and subcellular localization. Genes and Development 12: 3499-3511.
'Glycogen synthase kinase-3beta regulates cyclin D1 proteolysis and subcellular localization ' () 12 Genes and Development : 3499 -3511.
Fakhry, S., Sorrentini, I., Ricca, E., De Felice, M. and Baccigalupi, L., 2008. Characterization of spore forming Bacilli isolated from the human gastrointestinal tract. Journal of Applied Microbiology 105: 2178-2186.
'Characterization of spore forming Bacilli isolated from the human gastrointestinal tract ' () 105 Journal of Applied Microbiology : 2178 -2186.
Fujiya, M., Musch, M.W., Nakagawa, Y., Hu, S., Alverdy, J., Kohgo, Y., Schneewind, O., Jabri, B. and Chang, E.B., 2007. The Bacillus subtilis quorum-sensing molecule CSF contributes to intestinal homeostasis via OCTN2, a host cell membrane transporter. Cell Host and Microbe 1: 299-308.
'The Bacillus subtilis quorum-sensing molecule CSF contributes to intestinal homeostasis via OCTN2, a host cell membrane transporter ' () 1 Cell Host and Microbe : 299 -308.
Hevia, A., Delgado, S., Sanchez, B. and Margolles, A., 2015. Molecular players involved in the interaction between beneficial bacteria and the immune system. Frontiers in Microbiology 6: 1285.
'Molecular players involved in the interaction between beneficial bacteria and the immune system ' () 6 Frontiers in Microbiology : 1285.
Hong, H.A., To, E., Fakhry, S., Baccigalupi, L., Ricca, E. and Cutting, S.M., 2009. Defining the natural habitat of Bacillus spore-formers. Research in Microbiology 160: 375-379.
'Defining the natural habitat of Bacillus spore-formers ' () 160 Research in Microbiology : 375 -379.
Iyer, C., Kosters, A., Sethi, G., Kunnumakkara, A.B. and Aggarwal, B.B., 2008. Probiotic Lactobacillus reuteri promotes TNF-induced apoptosis in human myeloid leukemia-derived cells by modulation of NF-kB and MAPK signaling. Cellular Microbiology 10: 1442-1452.
'Probiotic Lactobacillus reuteri promotes TNF-induced apoptosis in human myeloid leukemia-derived cells by modulation of NF-kB and MAPK signaling ' () 10 Cellular Microbiology : 1442 -1452.
Kojima, K., Musch, M.W., Ren, H., Boone, D.L., Hendrickson, B.A., Ma, A. and Chang, E.B., 2003. Enteric flora and lymphocyte-derived cytokines determine expression of heat shock proteins in mouse colonic epithelial cells. Gastroenterology 124: 1395-1407
'Enteric flora and lymphocyte-derived cytokines determine expression of heat shock proteins in mouse colonic epithelial cells ' () 124 Gastroenterology : 1395 -1407.
Konishi, H., Fujiya, M. and Kohgo, Y., 2015. Host-microbe interactions via membrane transport systems. Environmental Microbiology 17: 931-937.
'Host-microbe interactions via membrane transport systems ' () 17 Environmental Microbiology : 931 -937.
Kravchenko, V.V., Kaufmann, G.F., Mathison, J.C., Scott, D.A., Katz, A.Z., Wood, M.R., Brogan, A.P., Lehmann, M., Mee, J.M. and Iwata, K., 2006. N-(3-oxo-acyl)homoserine lactones signal cell activation through a mechanism distinct from the canonical pathogen-associated molecular pattern recognition receptor pathways. Journal of Biological Chemistry 281: 28822-28830.
'N-(3-oxo-acyl)homoserine lactones signal cell activation through a mechanism distinct from the canonical pathogen-associated molecular pattern recognition receptor pathways ' () 281 Journal of Biological Chemistry : 28822 -28830.
Lanigan-Gerdes, S., Briceno, G., Dooley, A.N., Faull, K.F. and Lazazzera, B.A., 2008. Identification of residues important for cleavage of the extracellular signaling peptide CSF of Bacillus subtilis from its precursor protein. Journal of Bacteriology 190: 6668-6675.
'Identification of residues important for cleavage of the extracellular signaling peptide CSF of Bacillus subtilis from its precursor protein ' () 190 Journal of Bacteriology : 6668 -6675.
Lazazzera, B.A., Kurtser, I.G., McQuade, R.S. and Grossman, D.A., 1999. An autoregulatory circuit affecting peptide signaling in Bacillus subtilis. Journal of Bacteriology 181: 5193-5200.
'An autoregulatory circuit affecting peptide signaling in Bacillus subtilis. ' () 181 An autoregulatory circuit affecting peptide signaling in Bacillus subtilis. Journal of Bacteriology : 5193 -5200.
Lazazzera, B.A., Solomon, J.M. and Grossman, A.D., 1997. An exported peptide functions intracellularly to contribute to cell density signaling in B. subtilis. Cell 89: 917-925.
'An exported peptide functions intracellularly to contribute to cell density signaling in B. subtilis ' () 89 Cell : 917 -925.
Nishimura, T., Andoh, A., Nishida, A., Shioya, M., Koizumi, Y., Tsujikawa, T. and Fujiyama, Y., 2008. FR167653, a p38 mitogen-activated protein kinase inhibitor, aggravates experimental colitis in mice. World Journal of Gastroenterology 14: 5851-5856.
'FR167653, a p38 mitogen-activated protein kinase inhibitor, aggravates experimental colitis in mice ' () 14 World Journal of Gastroenterology : 5851 -5856.
Okamoto, K., Fujita, M., Nata, T., Ueno, N., Inaba, Y., Ishikawa, C., Ito, T., Moriichi, K., Tanabe, H., Mizukami, Y., Chang, E.B. and Kohgo, Y., 2012. Competence and sporulation factor derived from Bacillus subtilis improves epithelial cell injury in intestinal inflammation via immunomodulation and cytoprotection. International Journal of Colorectal Disease 27: 1039-1046.
'Competence and sporulation factor derived from Bacillus subtilis improves epithelial cell injury in intestinal inflammation via immunomodulation and cytoprotection ' () 27 International Journal of Colorectal Disease : 1039 -1046.
Rakoff-Nahoum, S. and Medzhitov, R., 2006. Role of the innate immune system and host-commensal mutualism. Current Topics in Microbiology and Immunology 308: 1-18.
'Role of the innate immune system and host-commensal mutualism ' () 308 Current Topics in Microbiology and Immunology : 1 -18.
Rhee, K.J., Sethupathi, P., Driks, A., Lanning, D.K. and Knight, K.L., 2004. Role of commensal bacteria in development of gut-associated lymphoid tissues and preimmune antibody repertoire. Journal of Immunology 172: 1118-1124.
'Role of commensal bacteria in development of gut-associated lymphoid tissues and preimmune antibody repertoire ' () 172 Journal of Immunology : 1118 -1124.
Segawa, S., Fujiya, M., Konishi, H., Ueno, N., Kobayashi, N., Shigyo, T. and Kohgo, Y., 2011. Probiotic-derived polyphosphate enhances the epithelial barrier function and maintains intestinal homeostasis through Integrin-p38 MAPK pathway. PLoS One 6: e23278.
'Probiotic-derived polyphosphate enhances the epithelial barrier function and maintains intestinal homeostasis through Integrin-p38 MAPK pathway ' () 6 PLoS One : e23278.
Sperandio, V., Torres, A.G., Jarvis, B., Nataro, J.P. and Kaper, J.B., 2003. Bacteria-host communication: the language of hormones. Proceedings of the National Academy of Sciences of the USA 100: 8951-8956.
'Bacteria-host communication: the language of hormones ' () 100 Proceedings of the National Academy of Sciences of the USA : 8951 -8956.
Solomon, J.M., Lazazzera, B.A. and Grossman, D.A., 1996. Purification and characterization of an extracellular peptide factor that affects two different developmental pathways in Bacillus subtilis. Genes and Development 10: 2014-2024.
'Purification and characterization of an extracellular peptide factor that affects two different developmental pathways in Bacillus subtilis ' () 10 Genes and Development : 2014 -2024.
Tao, Y., Drabik, K.A., Waypa, T.S., Musch, M.W., Alverdy, J.C., Schneewind, O., Chang, E.B. and Petrof, E.O., 2006. Soluble factors from Lactobacillus GG activate MAPKs and induce cytoprotective heat shock proteins in intestinal epithelial cells. American Journal of Physiology â Cell Physiology 290: C1018-C1030.
'Soluble factors from Lactobacillus GG activate MAPKs and induce cytoprotective heat shock proteins in intestinal epithelial cells ' () 290 American Journal of Physiology â Cell Physiology : C1018 -C1030.
Tortosa, P., Logsdon, L., Kraigher, B., Itoh, Y., Mandic-Mulec, I. and Dubnau, D., 2001. Specificity and genetic polymorphism of the Bacillus competence quorum-sensing system. Journal of Bacteriology 183: 451-460.
'Specificity and genetic polymorphism of the Bacillus competence quorum-sensing system ' () 183 Journal of Bacteriology : 451 -460.
Vary, P.S., 1994. Prime time for Bacillus megaterium. Microbiology 140: 1001-1013.
'Prime time for Bacillus megaterium ' () 140 Microbiology : 1001 -1013.
Vikstrom, E., Magnusson, K.E. and Pivorinas, A., 2005. The Pseudomonas aeruginosa quorum-sensing molecule N-(3-oxododecanoyl)-L homoserine lactone stimulates phagocytic activity in human macrophages through the p38 MAPK pathway. Microbes and Infection 7: 1512-1518.
'The Pseudomonas aeruginosa quorum-sensing molecule N-(3-oxododecanoyl)-L homoserine lactone stimulates phagocytic activity in human macrophages through the p38 MAPK pathway ' () 7 Microbes and Infection : 1512 -1518.
Vikstrom, E., Tafazoli, F. and Magnusson, K.E., 2006. Pseudomonas aeruginosa quorum sensing molecule N-(3 oxododecanoyl)-l-homoserine lactone disrupts epithelial barrier integrity of Caco-2 cells. FEBS Letter 580: 6921-6928.
'Pseudomonas aeruginosa quorum sensing molecule N-(3 oxododecanoyl)-l-homoserine lactone disrupts epithelial barrier integrity of Caco-2 cells ' () 580 FEBS Letter : 6921 -6928.
Yang, K., Hitomi, M. and Stacey, D.W., 2006. Variations in cyclin D1 levels through the cell cycle determine the proliferative fate of a cell. Cell Division 1: 32.
'Variations in cyclin D1 levels through the cell cycle determine the proliferative fate of a cell ' () 1 Cell Division : 32.
Youngman, P., Perkins, J.B. and Losick, R., 1984a. Construction of a cloning site near one end of Tn917 into which foreign DNA may be inserted without affecting transposition in Bacillus subtilis or expression of the transposon-borne erm gene. Plasmid 12: 1-9.
'Construction of a cloning site near one end of Tn917 into which foreign DNA may be inserted without affecting transposition in Bacillus subtilis or expression of the transposon-borne erm gene ' () 12 Plasmid : 1 -9.
Youngman, P., Perkins, J.B. and Losick, R., 1984b. A novel method for the rapid cloning in Escherichia coli of Bacillus subtilis chromosomal DNA adjacent to Tn917 insertion. Molecular Genetics and Genomics 195: 424-433.
'A novel method for the rapid cloning in Escherichia coli of Bacillus subtilis chromosomal DNA adjacent to Tn917 insertion ' () 195 Molecular Genetics and Genomics : 424 -433.
| å ¨é¨æé´ | è¿å»ä¸å¹´ | è¿å»30天 | |
|---|---|---|---|
| æè¦æµè§æ¬¡æ° | 370 | 130 | 15 |
| å ¨ææµè§æ¬¡æ° | 22 | 11 | 0 |
| PDFä¸è½½æ¬¡æ° | 29 | 14 | 0 |
The interaction between the enteric microbiota and intestinal cells often involves signal molecules that affect both microbial behaviour and host responses. Examples of such signal molecules are the molecules secreted by bacteria that induce quorum sensing mechanisms in the producing microorganism and signal transduction pathways in the host cells. The pentapeptide competence and sporulation factor (CSF) of Bacillus subtilis is a well characterized quorum sensing factor that controls competence and spore formation in the producing bacterium and induces cytoprotective heat shock proteins in intestinal epithelial cells. We analysed several Bacillus strains isolated from human ileal biopsies of healthy volunteers and observed that some of them were unable to produce CSF but still able to act in a CSF-like fashion on model intestinal epithelial cells. One of those strains belonging to the Bacillus megaterium species secreted at least two factors with effects on intestinal HT29 cells: a peptide smaller than 3 kDa able to induce heat shock protein 27 (hsp27) and p38-MAPK, and a larger molecule able to induce protein kinase B (PKB/Akt) with a pro-proliferative effect.
| å ¨é¨æé´ | è¿å»ä¸å¹´ | è¿å»30天 | |
|---|---|---|---|
| æè¦æµè§æ¬¡æ° | 370 | 130 | 15 |
| å ¨ææµè§æ¬¡æ° | 22 | 11 | 0 |
| PDFä¸è½½æ¬¡æ° | 29 | 14 | 0 |