Save

Use of T-RFLP and seven restriction enzymes to compare the faecal microbiota of obese and lean Japanese healthy men

于Beneficial Microbes
著者:
T. Kobayashi Miyagi University, 2-2-1 Hatadate, Taihaku-ku, Sendai City, Miyagi 982-0215, Japan
RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan

Search for other papers by T. Kobayashi in
Current site
Google Scholar
PubMed
Close
,
T. Osaki Kyorin University, School of Medicine, 6-20-2 Shinkawa Mitaka, Tokyo 181-8611, Japan

Search for other papers by T. Osaki in
Current site
Google Scholar
PubMed
Close
, and
S. Oikawa Miyagi University, 2-2-1 Hatadate, Taihaku-ku, Sendai City, Miyagi 982-0215, Japan

Search for other papers by S. Oikawa in
Current site
Google Scholar
PubMed
Close
Download Citation 获得许可

Access options

Get access to the full article by using one of the access options below.

Institutional Login

Log in with Open Athens, Shibboleth, or your institutional credentials

Login with Institutional Access

Purchase

Buy instant access (PDF download and unlimited online access):

€36.93

The composition of the intestinal microbiota of 92 healthy Japanese men was measured following consumption of identical meals for 3 days; terminal restriction fragment length polymorphisms were then used to analyse the DNA content of their faeces. The obtained operational taxonomic units (OTUs) were further analysed using seven restriction enzymes: 516f-BslI and -HaeIII, 27f-MspI and -AluI, and 35f-HhaI, -MspI and -AluI. Subjects were classified by their body mass index (BMI) as lean (<18.5) or obese (>25.0). OTUs were then analysed using data mining software. Pearson correlation coefficients on data mining results indicated only a weak relationship between BMI and OTU diversity. Specific OTUs attributed to lean and obese subjects were further examined by data mining with six groups of enzymes and closely related accession numbers for lean and obese subjects were successfully narrowed down. 16S rRNA sequences showed Bacillus spp., Erysipelothrix spp. and Holdemania spp. to be present among 30 bacterial candidates related to the lean group. Fifteen candidates were classified Firmicutes, one was classified as Chloroflexi, and the others were not classified. 45 Microbacteriaceae, 11 uncultured Actinobacterium, and 3 other families were present among the 119 candidate OTUs related to obesity. We conclude that the presence of Firmicutes and Actinobacteria may be related to the BMI of the subject.

内容统计数据

全部期间 过去一年 过去30天
摘要浏览次数 259 106 19
全文浏览次数 68 55 0
PDF下载次数 15 1 0