The allergenicity of β-lactoglobulin (β-Lg) was studied by using Ussing chamber in a murine model of β-Lg allergy supplemented with hydrolysates obtained after fermentation of milk for 48 h at 37 °C with Enterococcus faecalis DAPTO 512, isolated from cow milk and identified by 16S rDNA sequence analysis. Balb/c mice were sensitised intraperitoneally with β-Lg. Three groups of mice were formed: group 1, composed of naive mice used as control received only NaCl; group 2, positive control composed of mice sensitised intraperitoneally with β-Lg; group 3, formed by mice which were given hydrolysates of 48 h then sensitised with β-Lg. After 48 h of fermentation β-casein and β-Lg were degraded by E. faecalis DAPTO 512. β-Lg immunisation was associated with strong IgG and IgE production in case of positive controls and a significant increase in short current circuit (Isc) and high conductance (G) responses were observed. The control and the hydrolysate groups showed a significant decrease in the production of IgG and IgE anti β-Lg compared to the positive control. The allergenic potential of β-Lg was markedly reduced in the group that received hydrolysates (Isc and G remained unchanged after intestine challenge with β-Lg). The histological scrutiny showed villi atrophy, lymphocyte hyperplasia and a significant chorion detachment in the positive control group. In the group administered with hydrolysates of fermented milk, inflammatory signs were lower, the villi were long and thin and lymphocytes were less dense. The results showed that feeding of milk fermented with E. faecalis DAPTO 512 during 18 days prior to β-Lg allergy induction exerts a protecting effect on the murine intestine and induces a significant decrease in the β-Lg allergenicity.
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Addou-Benounane, S., Tomé, D., Kheroua, O. and Saidi, D., 2004. Parenteral immunization to β-lactoglobulin modifies the intestinal structure and mucosal electrical parameters in rabbit. International Immunopharmacology 4: 1559-1563.
'Parenteral immunization to β-lactoglobulin modifies the intestinal structure and mucosal electrical parameters in rabbit ' () 4 International Immunopharmacology : 1559 -1563.
Ahmadova, A., El-Ghaish, S., Choiset, Y, Rabesona, H., Drouet, M., Chobert, J.-M., Kuliev, A.A. and Haertlé, T., 2013. Modification of IgE binding to β- and α-S1-caseins by proteolytic activity of Lactobacillus helveticus A75. Journal of Food Biochemistry 37: 491-500.
'Modification of IgE binding to β- and α-S1-caseins by proteolytic activity of Lactobacillus helveticus A75 ' () 37 Journal of Food Biochemistry : 491 -500.
Bertrand-Harb, C., Ivanova, I.V., Dalgalarrondo, M. and Haertlé, T., 2003. Evolution of β-lactoglobulin and α-lactalbumin content during yoghurt fermentation. International Dairy Journal 13: 39-45.
'Evolution of β-lactoglobulin and α-lactalbumin content during yoghurt fermentation ' () 13 International Dairy Journal : 39 -45.
Beukes, E.M., Bester, H.B. and Mostert, J.F., 2001. The microbiology of South African traditional fermented milk. International Journal of Food Microbiology 63: 189-197.
'The microbiology of South African traditional fermented milk ' () 63 International Journal of Food Microbiology : 189 -197.
Bischoff, S. and Crowe, S.E., 2005. Gastrointestinal food allergy: new insights into pathophysiology and clinical perspectives. Gastroenterology 128: 1089-1113.
'Gastrointestinal food allergy: new insights into pathophysiology and clinical perspectives ' () 128 Gastroenterology : 1089 -1113.
Brigidi, P., Swennen, E., Vitali, B., Rossi, M. and Matteuzzi, M., 2003. PCR detection of Bifidobacterium strains and Streptococcus thermophilus in feces of human subjects after oral bacteriotherapy and yoghurt consumption. International Journal of Food Microbiology 81: 203-209.
'PCR detection of Bifidobacterium strains and Streptococcus thermophilus in feces of human subjects after oral bacteriotherapy and yoghurt consumption ' () 81 International Journal of Food Microbiology : 203 -209.
Carrasco de Mendoza, M., Meinardi, C.A., Meinardi, S.A. and Arturo, C., 1989. Actividad caseinolitica exocelular de enterococos para starters lacticos. Revista Argentina de Lactologıa 2: 27-37.
'Actividad caseinolitica exocelular de enterococos para starters lacticos ' () 2 Revista Argentina de Lactologıa : 27 -37.
Cocco, R.R., Jarvinen, K.M., Sampson, H.A. and Beyer, K., 2003. Mutational analysis of major, sequential Ig-E binding epitopes in αS1-casein, a major cow milk allergen. Journal of Allergy and Clinical Immunology 112: 433-437.
'Mutational analysis of major, sequential Ig-E binding epitopes in αS1-casein, a major cow milk allergen ' () 112 Journal of Allergy and Clinical Immunology : 433 -437.
Crowe, S.E., Sestini, P. and Perdue, M.H., 1990. Allergic reactions of rat jejunal mucosa. Ion transport responses to luminal antigen and inflammatory mediators. Gastroenterology 99: 74-82.
'Allergic reactions of rat jejunal mucosa. Ion transport responses to luminal antigen and inflammatory mediators ' () 99 Gastroenterology : 74 -82.
Dovat, A.M., Reinbold, G.W., Hammond, E.G. and Vedamuthu, E.R., 1970. Lipolytic and proteolytic activity of enterococci and lactic group streptococci isolate for young Cheddar cheese. Journal of Milk and Food Technology 33: 365-372.
'Lipolytic and proteolytic activity of enterococci and lactic group streptococci isolate for young Cheddar cheese ' () 33 Journal of Milk and Food Technology : 365 -372.
El-Ghaish, S., Ahmadova, A., Hadji-Sfaxi, I., El-Mecherfi, K.E., Bazukyan, I., Choiset, Y., Rabesona, A., Sitohy, M., Popov, Y.G., Kuliev, A. A., Mozzi, F., Chobert, J.-M. and Haertlé, T., 2011a. Potential use of lactic acid bacteria for reduction of allergenicity and for longer conservation of fermented foods. Trends in Food Science and Technology 22: 509-516.
'Potential use of lactic acid bacteria for reduction of allergenicity and for longer conservation of fermented foods ' () 22 Trends in Food Science and Technology : 509 -516.
El-Ghaish, S., Dalgalarrondo, M., Choiset, Y., Sitohy, M., Ivanova, I., Haertlé, T. and Chobert, J.-M., 2010a. Screening of strains of lactococci isolated from Egyptian dairy products for their proteolytic activity. Food Chemistry 120: 758-764.
'Screening of strains of lactococci isolated from Egyptian dairy products for their proteolytic activity ' () 120 Food Chemistry : 758 -764.
El-Ghaish, S., Dalgalarrondo, M., Choiset, Y., Sitohy, M., Ivanova, I., Haertlé, T. and Chobert, J.-M., 2010b. Characterization of a new isolate of Lactobacillus fermentum IFO 3956 from Egyptian Ras cheese with proteolytic activity. European Food Research and Technology 230: 635-643.
'Characterization of a new isolate of Lactobacillus fermentum IFO 3956 from Egyptian Ras cheese with proteolytic activity ' () 230 European Food Research and Technology : 635 -643.
El-Ghaish, S., Hadji-Sfaxi, I., Ahmadova, A., Choiset, Y., Rabesona, H., Sitohy, M., Haertlé, T. and Chobert, J.-M., 2011b. Characterization of two safe Enterococcus strains producing enterocins isolated from Egyptian dairy products. Beneficial Microbes 2: 15-27.
'Characterization of two safe Enterococcus strains producing enterocins isolated from Egyptian dairy products ' () 2 Beneficial Microbes : 15 -27.
El-Ghaish, S., Rabesona, H., Choiset, Y., Sitohy, M., Haertlé, T. and Chobert, J.-M., 2011c. Proteolysis by Lactobacillus fermentum IFO3956 isolated from Egyptian milk products decreases αS1-casein immuno-reactivity. Journal of Dairy Research 78: 203-210.
'Proteolysis by Lactobacillus fermentum IFO3956 isolated from Egyptian milk products decreases αS1-casein immuno-reactivity ' () 78 Journal of Dairy Research : 203 -210.
El-Mecherfi, K.E., Saidi, D., Kheroua, O., Boudra, G., Touhami, M., Rouaud, O., Curet, S. Choiset, Y., Rabesona, H., Chobert, J.-M. and Haertlé, T., 2011. Combined microwave and enzymatic treatments of beta-lactoglobulin and bovine whey proteins and their effect on the IgE immunoreactivity. European Food Research and Technology 233: 859-867.
'Combined microwave and enzymatic treatments of beta-lactoglobulin and bovine whey proteins and their effect on the IgE immunoreactivity ' () 233 European Food Research and Technology : 859 -867.
Heyman, M., Adrianstoa, M., Crain-Denoyelle, A.M. and Desjeux, J.F., 1990. Effect of oral or parenteral sensitization to cow milk on mucosal permeability in guinea pig. International Archives of Allergy and Immunology 92: 242-246.
'Effect of oral or parenteral sensitization to cow milk on mucosal permeability in guinea pig ' () 92 International Archives of Allergy and Immunology : 242 -246.
MacDonald, T.T., 1995. Evidence for cell-mediated hypersensitivity as an important pathogenic mechanism in food intolerance. Clinical and Experimental Allergy 25: 10-13.
'Evidence for cell-mediated hypersensitivity as an important pathogenic mechanism in food intolerance ' () 25 Clinical and Experimental Allergy : 10 -13.
Peñas, E., Préstamo, G., Baeza, M.L., Martínez-Molero, M.I. and Gomez, R., 2006. Effect of combined high pressure and enzymatic treatment on the hydrolysis and immuno-reactivity of dairy whey proteins. International Dairy Journal 16: 831-839.
'Effect of combined high pressure and enzymatic treatment on the hydrolysis and immuno-reactivity of dairy whey proteins ' () 16 International Dairy Journal : 831 -839.
Perdue, M.H. and Gall, D.G., 1986. Intestinal anaphylaxis in the rat jejunal response to in vitro antigen exposure. American Journal of Physiology 250: G427-G431.
'Intestinal anaphylaxis in the rat jejunal response to in vitro antigen exposure ' () 250 American Journal of Physiology : G427 -G431.
Pescuma, M., Hébert, E. M., Rabesona, H., Drouet, M., Choiset, Y., Haertlé, T., Mozzi, F., Font de Valdez, G. and Chobert, J.-M., 2011. Proteolytic action of Lactobacillus delbrueckii subsp. bulgaricus CRL 656 reduces antigenic response to bovine β-lactoglobulin. Food Chemistry 127: 487-492.
'Proteolytic action of Lactobacillus delbrueckii subsp. bulgaricus CRL 656 reduces antigenic response to bovine β-lactoglobulin ' () 127 Food Chemistry : 487 -492.
Pescuma, M., Hébert, E.M., Mozzi, F. and Font de Valdez, G., 2010. Functional fermented whey-based beverage using lactic acid bacteria. International Journal of Food Microbiology 141: 73-81.
'Functional fermented whey-based beverage using lactic acid bacteria ' () 141 International Journal of Food Microbiology : 73 -81.
Sarantinopoulos, P., Andrighetto, C., Georgalaki, M. D., Rea, M. C., Lombardi, A., Cogan, T. M., Kalantzopoulos, G. and Tsakalidou, E., 2001. Biochemical properties of enterococci relevant to their technological performance. International Dairy Journal 11: 621-647.
'Biochemical properties of enterococci relevant to their technological performance ' () 11 International Dairy Journal : 621 -647.
Somkuti, G.A. and Babel, F.J., 1969. Hydrolytic breakdown of casein by a proteinase of Streptococcus faecalis var. liquefaciens. Journal of Dairy Science 52: 1186-1191.
'Hydrolytic breakdown of casein by a proteinase of Streptococcus faecalis var. liquefaciens ' () 52 Journal of Dairy Science : 1186 -1191.
Strobel, S. and Hourihane, J.O.B., 2001. Gastrointestinal allergy: clinical symptoms and immunological mechanisms. Pediatric Allergy and Immunology 12: 43-46.
'Gastrointestinal allergy: clinical symptoms and immunological mechanisms ' () 12 Pediatric Allergy and Immunology : 43 -46.
Untersmayr, E. and Jensen-Jarolim, E., 2006. Mechanism of type I food allergy. Pharmacology and Therapeutics 112: 787-798.
'Mechanism of type I food allergy ' () 112 Pharmacology and Therapeutics : 787 -798.
Weisburg, W.G., Barns, S.M., Pelletier, D.A. and Lane, D.J., 1991. 16S ribosomal DNA amplification for phylogenetic study. Journal of Bacteriology 173: 697-703.
'16S ribosomal DNA amplification for phylogenetic study ' () 173 Journal of Bacteriology : 697 -703.
Wroblewska, B., Jedrychowski, L. and Bielecka, M., 1995. The effect of selected microorganisms on the presence of immunoreactive fractions in cow and goat milks. Polish Journal of Food and Nutrition Sciences 44/45: 21-28.
'The effect of selected microorganisms on the presence of immunoreactive fractions in cow and goat milks ' () 44/45 Polish Journal of Food and Nutrition Sciences : 21 -28.
Zellal, D., Kaddouri, H., Grar, H., Belarbi, H., Kheroua, O. and Saidi, D., 2011. Allergenic changes in β-lactoglobulin induced by microwave irradiation under different pH conditions. Food and Agricultural Immunology 22: 355-363.
'Allergenic changes in β-lactoglobulin induced by microwave irradiation under different pH conditions ' () 22 Food and Agricultural Immunology : 355 -363.
| All Time | Past 365 days | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 342 | 159 | 22 |
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| PDF Views & Downloads | 4 | 2 | 2 |
The allergenicity of β-lactoglobulin (β-Lg) was studied by using Ussing chamber in a murine model of β-Lg allergy supplemented with hydrolysates obtained after fermentation of milk for 48 h at 37 °C with Enterococcus faecalis DAPTO 512, isolated from cow milk and identified by 16S rDNA sequence analysis. Balb/c mice were sensitised intraperitoneally with β-Lg. Three groups of mice were formed: group 1, composed of naive mice used as control received only NaCl; group 2, positive control composed of mice sensitised intraperitoneally with β-Lg; group 3, formed by mice which were given hydrolysates of 48 h then sensitised with β-Lg. After 48 h of fermentation β-casein and β-Lg were degraded by E. faecalis DAPTO 512. β-Lg immunisation was associated with strong IgG and IgE production in case of positive controls and a significant increase in short current circuit (Isc) and high conductance (G) responses were observed. The control and the hydrolysate groups showed a significant decrease in the production of IgG and IgE anti β-Lg compared to the positive control. The allergenic potential of β-Lg was markedly reduced in the group that received hydrolysates (Isc and G remained unchanged after intestine challenge with β-Lg). The histological scrutiny showed villi atrophy, lymphocyte hyperplasia and a significant chorion detachment in the positive control group. In the group administered with hydrolysates of fermented milk, inflammatory signs were lower, the villi were long and thin and lymphocytes were less dense. The results showed that feeding of milk fermented with E. faecalis DAPTO 512 during 18 days prior to β-Lg allergy induction exerts a protecting effect on the murine intestine and induces a significant decrease in the β-Lg allergenicity.
| All Time | Past 365 days | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 342 | 159 | 22 |
| Full Text Views | 15 | 1 | 1 |
| PDF Views & Downloads | 4 | 2 | 2 |