Quantification of Blautia wexlerae and Blautia luti in human faeces by real-time PCR using specific primers
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Department of Oriental Medicine Resources, College of Environmental & Bioresource Sciences, Chonbuk National University, Iksan, Jeonbuk 570-752, Korea
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The Clostridium coccoides group, including the genus Blautia and other genera, is one of the predominant bacterial groups in the human intestine. We re-examined 266 human faecal clones and 58 isolates in the C. coccoides group isolated by Hayashi et al. (2002) in order to elucidate the detailed distribution of Blautia wexlerae and Blautia luti in human faeces. Subsequently, we designed a primer pair specific for B. wexlerae and B. luti based on the 16S ribosomal RNA (16S rRNA) gene sequence. The number of B. wexlerae and B. luti in faecal samples of 12 healthy Japanese subjects was examined by real-time PCR assay. The number of the C. coccoides group in the 12 faecal samples was also determined using C. coccoides group-specific primers. Re-examination of the human faecal clones and isolates revealed that B. wexlerae and B. luti accounted for 19.5% of the clones and 25.9% of the isolates. B. wexlerae and B. luti were detected in all faecal samples with 5.3±3.2×109 cells/g faeces (wet weight, average ± standard deviation) as assessed by real-time PCR. Furthermore, B. wexlerae and B. luti constituted 32.3±12.7% (average ± standard deviation) of the C. coccoides group (1.7±0.8×1010 cells/g faeces). This demonstrates that B. wexlerae and B. luti were presented in human faeces with a high frequency as the dominant bacteria.
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Quantification of Blautia wexlerae and Blautia luti in human faeces by real-time PCR using specific primers
In: Beneficial MicrobesSearch for other papers by M. Touyama in
Current site
Google Scholar
PubMed
Department of Oriental Medicine Resources, College of Environmental & Bioresource Sciences, Chonbuk National University, Iksan, Jeonbuk 570-752, Korea
Search for other papers by J.S. Jin in
Current site
Google Scholar
PubMed
Search for other papers by R. Kibe in
Current site
Google Scholar
PubMed
Search for other papers by H. Hayashi in
Current site
Google Scholar
PubMed
Search for other papers by Y. Benno in
Current site
Google Scholar
PubMed
The Clostridium coccoides group, including the genus Blautia and other genera, is one of the predominant bacterial groups in the human intestine. We re-examined 266 human faecal clones and 58 isolates in the C. coccoides group isolated by Hayashi et al. (2002) in order to elucidate the detailed distribution of Blautia wexlerae and Blautia luti in human faeces. Subsequently, we designed a primer pair specific for B. wexlerae and B. luti based on the 16S ribosomal RNA (16S rRNA) gene sequence. The number of B. wexlerae and B. luti in faecal samples of 12 healthy Japanese subjects was examined by real-time PCR assay. The number of the C. coccoides group in the 12 faecal samples was also determined using C. coccoides group-specific primers. Re-examination of the human faecal clones and isolates revealed that B. wexlerae and B. luti accounted for 19.5% of the clones and 25.9% of the isolates. B. wexlerae and B. luti were detected in all faecal samples with 5.3±3.2×109 cells/g faeces (wet weight, average ± standard deviation) as assessed by real-time PCR. Furthermore, B. wexlerae and B. luti constituted 32.3±12.7% (average ± standard deviation) of the C. coccoides group (1.7±0.8×1010 cells/g faeces). This demonstrates that B. wexlerae and B. luti were presented in human faeces with a high frequency as the dominant bacteria.
| All Time | Past 365 days | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 1563 | 509 | 66 |
| Full Text Views | 82 | 33 | 1 |
| PDF Views & Downloads | 133 | 58 | 3 |
