Lactobacillus plantarum P-8 (Lp-8) is a novel probiotic bacterium showing beneficial effects in human. The current study is a follow-up work of a previous clinical trial, and it aimed at understanding the mechanism of the observed desirable effects by evaluating the change in faecal bacterial structure at phylogenetic level. The genomic DNAs used for 16S rRNA polymerase chain reaction (PCR)-pyrosequencing were obtained from the previous clinical trial. Briefly, 33 recruited subjects were given a single daily oral dose of Lp-8 (6×1010 cfu) for 4 weeks. Faecal genomic DNAs were extracted from samples collected before (day 0) and after (day 28) Lp-8 consumption from the subjects. Our results show that the consumption of Lp-8 did not result in major change in the faecal bacterial structure by principal coordinate analysis. However, multivariate analysis of variance and analysis of UniFrac metric distances at day 0 and day 28 revealed significant difference in the faecal bacterial structure upon Lp-8 treatment. Thus, the difference lied on the less abundant rather than the predominant lineages. At genus level, the relative abundance of 5 Firmicutes genera (Leuconostoc, Lactobacillus, Sporacetigenium, Blautia and Staphylococcus) significantly increased after Lp-8 consumption, whereas 3 Proteobacteria genera (Shigella, Escherichia and Enterobacter) decreased (P<0.05). 60 key responding lineages could be identified at operational taxonomic unit (OTU) level by redundancy analysis. Among them, the abundance of 18 OTUs showed significant difference (P<0.05), while 12 OTUs exhibited modest change (0.05<P<0.01) upon treatment. Our study identified a shift in the faecal bacterial structure upon Lp-8 administration, which may explain some of the previously observed beneficial effects.
Purchase
Buy instant access (PDF download and unlimited online access):
Institutional Login
Log in with Open Athens, Shibboleth, or your institutional credentials
Personal login
Log in with your brill.com account
Ahmed, M., Prasad, J., Gill, H., Stevenson, L. and Gopal, P., 2007. Impact of consumption of different levels of Bifidobacterium lactis HN019 on the intestinal microflora of elderly human subjects. Journal of Nutrition, Health and Aging 11: 26-31.
'Impact of consumption of different levels of Bifidobacterium lactis HN019 on the intestinal microflora of elderly human subjects ' () 11 Journal of Nutrition, Health and Aging : 26 -31.
Antharam, V.C., Li, E.C., Ishmael, A., Sharma, A., Mai, V., Rand, K.H., Wang, G.P., 2013. Intestinal dysbiosis and depletion of butyrogenic bacteria in Clostridium difficile infection and nosocomial diarrhea. Journal of Clinical Microbiology 51: 2884-2892.
'Intestinal dysbiosis and depletion of butyrogenic bacteria in Clostridium difficile infection and nosocomial diarrhea ' () 51 Journal of Clinical Microbiology : 2884 -2892.
Bao, Y., Wang, Z., Zhang, Y., Zhang, J., Wang, L., Dong, X., Su, F., Yao, G., Wang, S. and Zhang, H., 2012. Effect of Lactobacillus plantarum P-8 on lipid metabolism in hyperlipidemic rat model. European Journal of Lipid Science and Technology 114: 1230-1236.
'Effect of Lactobacillus plantarum P-8 on lipid metabolism in hyperlipidemic rat model ' () 114 European Journal of Lipid Science and Technology : 1230 -1236.
Bao, Y., Zhang, Y., Li, H., Liu, Y., Wang, S., Dong, X., Su, F., Yao, G., Sun, T. and Zhang, H., 2011. In vitro screen of Lactobacillus plantarum as probiotic bacteria and their fermented characteristics in soymilk. Annals of Microbiology 62: 1311-1320.
'In vitro screen of Lactobacillus plantarum as probiotic bacteria and their fermented characteristics in soymilk ' () 62 Annals of Microbiology : 1311 -1320.
Begley, M., Hill, C. and Gahan, C.G.M., 2006. Bile salt hydrolase activity in probiotics. Applied and Environmental Microbiology 72: 1729-1738.
'Bile salt hydrolase activity in probiotics ' () 72 Applied and Environmental Microbiology : 1729 -1738.
Bernstein, C., Holubec, H., Bhattacharyya, A.K., Nguyen, H., Payne, C.M., Zaitlin, B. and Bernstein, H., 2011. Carcinogenicity of deoxycholate, a secondary bile acid. Archives of Toxicology 85: 863-871.
'Carcinogenicity of deoxycholate, a secondary bile acid ' () 85 Archives of Toxicology : 863 -871.
Cox, M.J., Huang, Y.J., Fujimura, K.E., Liu, J.T., McKean, M., Boushey, H.A., Segal, M.R., Brodie, E.L., Cabana, M.D. and Lynch, S.V., 2010. Lactobacillus casei abundance is associated with profound shifts in the infant gut microbiome. PLoS ONE 5: e8745.
'Lactobacillus casei abundance is associated with profound shifts in the infant gut microbiome ' () 5 PLoS ONE : e8745.
De Moreno de LeBlanc, A., Dogi, C.A., Galdeano, C., Carmuega, E., Weill, R. and Perdigón, G., 2008. Effect of the administration of a fermented milk containing Lactobacillus casei DN-114001 on intestinal microbiota and gut associated immune cells of nursing mice and after weaning until immune maturity. BMC Immunology 9: 27.
'Effect of the administration of a fermented milk containing Lactobacillus casei DN-114001 on intestinal microbiota and gut associated immune cells of nursing mice and after weaning until immune maturity ' () 9 BMC Immunology : 27.
De Vries, M.C., Vaughan, E.E., Kleerebezem, M. and De Vos, W.M., 2006. Lactobacillus plantarum – survival, functional and potential probiotic properties in the human intestinal tract. International Dairy Journal 16: 1018-1028.
'Lactobacillus plantarum – survival, functional and potential probiotic properties in the human intestinal tract ' () 16 International Dairy Journal : 1018 -1028.
Dethlefsen, L. and Relman, D.A., 2011. Incomplete recovery and individualized responses of the human distal gut microbiota to repeated antibiotic perturbation. Proceedings of the National Academy of Sciences of the USA 108 Suppl. 1: 4554-4561.
'Incomplete recovery and individualized responses of the human distal gut microbiota to repeated antibiotic perturbation ' () 1 Proceedings of the National Academy of Sciences of the USA 108 Suppl. : 4554 -4561.
Duncan, S.H. and Flint, H.J., 2013. Probiotics and prebiotics and health in ageing populations. Maturitas 75: 44-50.
'Probiotics and prebiotics and health in ageing populations ' () 75 Maturitas : 44 -50.
Hammer, Ø., Harper, D.A. and Ryan, P.D., 2001. PAST: paleontological statistics software package for education and data analysis. Palaeontologia Electronica 4: 9.
'PAST: paleontological statistics software package for education and data analysis ' () 4 Palaeontologia Electronica : 9.
Hosseini, E., Grootaert, C., Verstraete, W. and Van de Wiele, T., 2011. Propionate as a health-promoting microbial metabolite in the human gut: Nutrition Reviews 69: 245-258.
'Propionate as a health-promoting microbial metabolite in the human gut: Nutrition Reviews ' () 69 : 245 -258.
Joyce, S.A., Gahan, C.G.M., 2014. The gut microbiota and the metabolic health of the host. Current Opinion in Gastroenterology 30: 120-127.
'The gut microbiota and the metabolic health of the host ' () 30 Current Opinion in Gastroenterology : 120 -127.
Kirkman, T.W., 1996. Statistics to use. Available at: http://www.physics.csbsju.edu/stats/.
'Statistics to use ', ().
Lahtinen, S.J., Forssten, S., Aakko, J., Granlund, L., Rautonen, N., Salminen, S., Viitanen, M. and Ouwehand, A.C., 2011. Probiotic cheese containing Lactobacillus rhamnosus HN001 and Lactobacillus acidophilus NCFM® modifies subpopulations of fecal lactobacilli and Clostridium difficile in the elderly. Age 34: 133-143.
'Probiotic cheese containing Lactobacillus rhamnosus HN001 and Lactobacillus acidophilus NCFM® modifies subpopulations of fecal lactobacilli and Clostridium difficile in the elderly ' () 34 Age : 133 -143.
McNulty, N.P., Yatsunenko, T., Hsiao, A., Faith, J.J., Muegge, B.D., Goodman, A.L., Henrissat, B., Oozeer, R., Cools-Portier, S., Gobert, G., Chervaux, C., Knights, D., Lozupone, C.A., Knight, R., Duncan, A.E., Bain, J.R., Muehlbauer, M.J., Newgard, C.B., Heath, A.C. and Gordon, J.I., 2011. The impact of a consortium of fermented milk strains on the gut microbiome of gnotobiotic mice and monozygotic twins. Science Translational Medicine 3: 106ra106.
'The impact of a consortium of fermented milk strains on the gut microbiome of gnotobiotic mice and monozygotic twins ' () 3 Science Translational Medicine : 106ra106.
Morgan, X.C., Tickle, T.L., Sokol, H., Gevers, D., Devaney, K.L., Ward, D.V., Reyes, J.A., Shah, S.A., LeLeiko, N., Snapper, S.B., Bousvaros, A., Korzenik, J., Sands, B.E., Xavier, R.J. and Huttenhower, C., 2012. Dysfunction of the intestinal microbiome in inflammatory bowel disease and treatment. Genome Biology 13: R79.
'Dysfunction of the intestinal microbiome in inflammatory bowel disease and treatment ' () 13 Genome Biology : R79.
Nagata, S., Asahara, T., Ohta, T., Yamada, T., Kondo, S., Bian, L., Wang, C., Yamashiro, Y. and Nomoto, K., 2011. Effect of the continuous intake of probiotic-fermented milk containing Lactobacillus casei strain Shirota on fever in a mass outbreak of norovirus gastroenteritis and the faecal microflora in a health service facility for the aged. British Journal of Nutrition 106: 549-556.
'Effect of the continuous intake of probiotic-fermented milk containing Lactobacillus casei strain Shirota on fever in a mass outbreak of norovirus gastroenteritis and the faecal microflora in a health service facility for the aged ' () 106 British Journal of Nutrition : 549 -556.
Rampelli, S., Candela, M., Severgnini, M., Biagi, E., Turroni, S., Roselli, M., Carnevali, P., Donini, L. and Brigidi, P., 2013. A probiotics-containing biscuit modulates the intestinal microbiota in the elderly. Journal of Nutrition, Health and Aging 17: 166-172.
'A probiotics-containing biscuit modulates the intestinal microbiota in the elderly ' () 17 Journal of Nutrition, Health and Aging : 166 -172.
Riboulet-Bisson, E., Sturme, M.H.J., Jeffery, I.B., O’Donnell, M.M., Neville, B.A., Forde, B.M., Claesson, M.J., Harris, H., Gardiner, G.E., Casey, P.G., Lawlor, P.G., O’Toole, P.W. and Ross, R.P., 2012. Effect of Lactobacillus salivarius bacteriocin Abp118 on the mouse and pig intestinal microbiota. PLoS ONE 7: e31113.
'Effect of Lactobacillus salivarius bacteriocin Abp118 on the mouse and pig intestinal microbiota ' () 7 PLoS ONE : e31113.
Ridlon, J.M., Kang, D.J. and Hylemon, P.B., 2006. Bile salt biotransformations by human intestinal bacteria. Journal of Lipid Research 47: 241-259.
'Bile salt biotransformations by human intestinal bacteria ' () 47 Journal of Lipid Research : 241 -259.
Rubio, R., Aymerich, T., Bover-Cid, S., Guàrdia, M.D., Arnau, J. and Garriga, M., 2013. Probiotic strains Lactobacillus plantarum 299V and Lactobacillus rhamnosus GG as starter cultures for fermented sausages. LWT – Food Science Technology 54: 51-56.
'Probiotic strains Lactobacillus plantarum 299V and Lactobacillus rhamnosus GG as starter cultures for fermented sausages ' () 54 LWT – Food Science Technology : 51 -56.
Veiga, P., Gallini, C.A., Beal, C., Michaud, M., Delaney, M.L., DuBois, A., Khlebnikov, A., Van Hylckama Vlieg, J.E.T., Punit, S., Glickman, J.N., Onderdonk, A., Glimcher, L.H. and Garrett, W.S., 2010. Bifidobacterium animalis subsp. lactis fermented milk product reduces inflammation by altering a niche for colitogenic microbes. Proceedings of the National Academy of Sciences of the USA 107: 18132-18137.
'Bifidobacterium animalis subsp. lactis fermented milk product reduces inflammation by altering a niche for colitogenic microbes ' () 107 Proceedings of the National Academy of Sciences of the USA : 18132 -18137.
Wang, J., Tang, H., Zhang, C., Zhao, Y., Derrien, M., Rocher, E., Van Hylckama Vlieg, J.E., Strissel, K., Zhao, L., Obin, M. and Shen, J., in press. Modulation of gut microbiota during probiotic-mediated attenuation of metabolic syndrome in high fat diet-fed mice. ISME Journal, in press. DOI: http://dx. doi.org/10.1038/ismej.2014.99.
'Modulation of gut microbiota during probiotic-mediated attenuation of metabolic syndrome in high fat diet-fed mice ', in ISME Journal , ().
Wang, L., Zhang, J., Guo, Z., Kwok, L., Ma, C., Zhang, W., Lv, Q., Huang, W. and Zhang, H., 2013. The impact of oral consumption of the probiotic Lactobacillus plantarum P-8 on the faecal microbiota, SIgA, SCFAs and TBAs of subjects of different age. Nutrition 30: 776-783.
'The impact of oral consumption of the probiotic Lactobacillus plantarum P-8 on the faecal microbiota, SIgA, SCFAs and TBAs of subjects of different age ' () 30 Nutrition : 776 -783.
Wang, Z., Bao, Y., Zhang, Y., Zhang, J., Yao, G., Wang, S. and Zhang, H., 2012. Effect of soymilk fermented with Lactobacillus plantarum P-8 on lipid metabolism and fecal microbiota in experimental hyperlipidemic rats. Food Biophysics 8: 43-49.
'Effect of soymilk fermented with Lactobacillus plantarum P-8 on lipid metabolism and fecal microbiota in experimental hyperlipidemic rats ' () 8 Food Biophysics : 43 -49.
Winter, S.E., Lopez, C.A. and Bäumler, A.J., 2013a. The dynamics of gut-associated microbial communities during inflammation. EMBO Reports 14: 319-327.
'The dynamics of gut-associated microbial communities during inflammation ' () 14 EMBO Reports : 319 -327.
Winter, S.E., Winter, M.G., Xavier, M.N., Thiennimitr, P., Poon, V., Keestra, A.M., Laughlin, R.C., Gomez, G., Wu, J., Lawhon, S.D., Popova, I.E., Parikh, S.J., Adams, L.G., Tsolis, R.M., Stewart, V.J. and Baumler, A.J., 2013b. Host-derived nitrate boosts growth of E. coli in the inflamed gut. Science 339: 708-711.
'Host-derived nitrate boosts growth of E. coli in the inflamed gut ' () 339 Science : 708 -711.
Yin, X., Yan, Y., Kim, E.B., Lee, B. and Marco, M.L., 2014. Short communication: effect of milk and milk containing Lactobacillus casei on the intestinal microbiota of mice. Journal of Dairy Science 97: 2049-2055.
'Short communication: effect of milk and milk containing Lactobacillus casei on the intestinal microbiota of mice ' () 97 Journal of Dairy Science : 2049 -2055.
Zhang, J., Wang, L., Guo, Z., Sun, Z., Gesudu, Q., Kwok, L.Y., Menghebilige and Zhang, H., 2014. 454 pyrosequencing reveals changes in the faecal microbiota of adults consuming Lactobacillus casei Zhang. FEMS Microbiology Ecology 88: 612-622.
'454 pyrosequencing reveals changes in the faecal microbiota of adults consuming Lactobacillus casei Zhang ' () 88 FEMS Microbiology Ecology : 612 -622.
Zvanych, R., Lukenda, N., Kim, J.J., Li, X., Petrof, E.O., Khan, W.I. and Magarvey, N.A., 2014. Small molecule immunomodulins from cultures of the human microbiome member Lactobacillus plantarum. Journal of Antibiotics 67: 85-88.
'Small molecule immunomodulins from cultures of the human microbiome member Lactobacillus plantarum ' () 67 Journal of Antibiotics : 85 -88.
| All Time | Past 365 days | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 585 | 204 | 14 |
| Full Text Views | 108 | 85 | 0 |
| PDF Views & Downloads | 39 | 13 | 0 |
Lactobacillus plantarum P-8 (Lp-8) is a novel probiotic bacterium showing beneficial effects in human. The current study is a follow-up work of a previous clinical trial, and it aimed at understanding the mechanism of the observed desirable effects by evaluating the change in faecal bacterial structure at phylogenetic level. The genomic DNAs used for 16S rRNA polymerase chain reaction (PCR)-pyrosequencing were obtained from the previous clinical trial. Briefly, 33 recruited subjects were given a single daily oral dose of Lp-8 (6×1010 cfu) for 4 weeks. Faecal genomic DNAs were extracted from samples collected before (day 0) and after (day 28) Lp-8 consumption from the subjects. Our results show that the consumption of Lp-8 did not result in major change in the faecal bacterial structure by principal coordinate analysis. However, multivariate analysis of variance and analysis of UniFrac metric distances at day 0 and day 28 revealed significant difference in the faecal bacterial structure upon Lp-8 treatment. Thus, the difference lied on the less abundant rather than the predominant lineages. At genus level, the relative abundance of 5 Firmicutes genera (Leuconostoc, Lactobacillus, Sporacetigenium, Blautia and Staphylococcus) significantly increased after Lp-8 consumption, whereas 3 Proteobacteria genera (Shigella, Escherichia and Enterobacter) decreased (P<0.05). 60 key responding lineages could be identified at operational taxonomic unit (OTU) level by redundancy analysis. Among them, the abundance of 18 OTUs showed significant difference (P<0.05), while 12 OTUs exhibited modest change (0.05<P<0.01) upon treatment. Our study identified a shift in the faecal bacterial structure upon Lp-8 administration, which may explain some of the previously observed beneficial effects.
| All Time | Past 365 days | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 585 | 204 | 14 |
| Full Text Views | 108 | 85 | 0 |
| PDF Views & Downloads | 39 | 13 | 0 |