Impact of different cryoprotectants on the survival of freeze-dried Lactobacillus rhamnosus and Lactobacillus casei/paracasei during long-term storage
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The production of long shelf-life highly concentrated dried probiotic/starter cultures is of paramount importance for the food industry. The aim of the present study was to evaluate the protective effect of glucose, lactose, trehalose, and skim milk applied alone or combined upon the survival of potentially probiotic Lactobacillus rhamnosus CTC1679, Lactobacillus casei/paracasei CTC1677 and L. casei/paracasei CTC1678 during freeze-drying and after 39 weeks of storage at 4 and 22 °C. Immediately after freeze-drying, the percentage of survivors was very high (≥94%) and only slight differences were observed among strains and cryoprotectants. In contrast, during storage, survival in the dried state depended on the cryoprotectant, temperature and strain. For all the protectants assayed, the stability of the cultures was remarkably higher when stored under refrigeration (4 °C). Under these conditions, skim milk alone or supplemented with trehalose or lactose showed the best performance (reductions ≤0.9 log units after 39 weeks of storage). The lowest survival was observed during non-refrigerated storage and with glucose and glucose plus milk; no viable cells left at the end of the storage period. Thus, freeze-drying in the presence of appropriate cryoprotectants allows the production of long shelf-life highly concentrated dried cultures ready for incorporation in high numbers into food products as starter/potential probiotic cultures.
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Bergenholtz, A.S., Wessman, P., Wuttke, A. and Hakansson, S., 2012. A case study on stress preconditioning of a Lactobacillus strain prior to freeze-drying. Cryobiology 64: 152-159.
'A case study on stress preconditioning of a Lactobacillus strain prior to freeze-drying ' () 64 Cryobiology : 152 -159.
-
Carvalho, A.S., Silva, J., Ho, P., Teixeira, P., Malcata, F.X. and Gibbs, P., 2002. Survival of freeze-dried Lactobacillus plantarum and Lactobacillus rhamnosus during storage in the presence of protectants. Biotechnology Letters 24: 1587-1591.
'Survival of freeze-dried Lactobacillus plantarum and Lactobacillus rhamnosus during storage in the presence of protectants ' () 24 Biotechnology Letters : 1587 -1591.
-
Carvalho, A.S., Silva, J., Ho, P., Teixeira, P., Malcata, F.X. and Gibbs, P., 2003. Effects of addition of sucrose and salt, and of starvation upon thermotolerance and survival during storage of freeze-dried Lactobacillus delbrueckii ssp. bulgaricus. Journal of Food Science 68: 2538-2541.
'Effects of addition of sucrose and salt, and of starvation upon thermotolerance and survival during storage of freeze-dried Lactobacillus delbrueckii ssp. bulgaricus ' () 68 Journal of Food Science : 2538 -2541.
-
Carvalho, A.S., Silva, J., Ho, P., Teixeira, P., Malcata, F.X. and Gibbs, P., 2004a. Effects of various sugars added to growth and drying media upon thermotolerance and survival throughout storage of freeze-dried Lactobacillus delbrueckii ssp. bulgaricus. Biotechnology Progress 20: 248-254.
'Effects of various sugars added to growth and drying media upon thermotolerance and survival throughout storage of freeze-dried Lactobacillus delbrueckii ssp. bulgaricus ' () 20 Biotechnology Progress : 248 -254.
-
Carvalho, A.S., Silva, J., Ho, P., Teixeira, P., Malcata, F.X. and Gibbs, P., 2004b. Relevant factors for the preparation of freeze-dried lactic acid bacteria. International Dairy Journal 14: 835-847.
'Relevant factors for the preparation of freeze-dried lactic acid bacteria ' () 14 International Dairy Journal : 835 -847.
-
Castro, H.P., Teixeira, P.M. and Kirby, R., 1995. Storage of lyophilized cultures of Lactobacillus bulgaricus under different relative humidities and atmospheres. Applied Microbiology and Biotechnology 44: 172-176.
'Storage of lyophilized cultures of Lactobacillus bulgaricus under different relative humidities and atmospheres ' () 44 Applied Microbiology and Biotechnology : 172 -176.
-
Castro, H.P., Teixeira, P.M. and Kirby, R., 1997. Evidence of membrane damage in Lactobacillus bulgaricus following freeze drying. Journal of Applied Microbiology 82: 87-94.
'Evidence of membrane damage in Lactobacillus bulgaricus following freeze drying ' () 82 Journal of Applied Microbiology : 87 -94.
-
Corcoran, B.M., Ross, R.P., Fitzgerald, G.F. and Stanton, C., 2004. Comparative survival of probiotic lactobacilli spray-dried in the presence of prebiotic substances. Journal of Applied Microbiology 96: 1024-1039.
'Comparative survival of probiotic lactobacilli spray-dried in the presence of prebiotic substances ' () 96 Journal of Applied Microbiology : 1024 -1039.
-
Higl, B., Kurtmann, L., Carlsen, C.U., Ratjen, J., Först, P., Skibsted, L.H., Kulozik, U. and Risbo, J., 2007. Impact of water activity, temperature, and physical state on the storage stability of Lactobacillus paracasei ssp. paracasei freeze-dried in a lactose matrix. Biotechnology Progress 23: 794-800.
'Impact of water activity, temperature, and physical state on the storage stability of Lactobacillus paracasei ssp. paracasei freeze-dried in a lactose matrix ' () 23 Biotechnology Progress : 794 -800.
-
Hubálek, Z., 2003. Protectants used in the cryopreservation of microorganisms. Cryobiology 46: 205-229.
'Protectants used in the cryopreservation of microorganisms ' () 46 Cryobiology : 205 -229.
-
Leroy, F., Verluyten, J. and De Vuyst, L., 2006. Functional meat starter cultures for improved sausage fermentation. International Journal of Food Microbiology 106: 270-285.
'Functional meat starter cultures for improved sausage fermentation ' () 106 International Journal of Food Microbiology : 270 -285.
-
Miao, S., Mills, S., Stanton, C., Fitzgerald, G.F., Roos, Y. and Ross, R.P., 2008. Effect of disaccharides on survival during storage of freeze dried probiotics. Dairy Science and Technology 88: 19-30.
'Effect of disaccharides on survival during storage of freeze dried probiotics ' () 88 Dairy Science and Technology : 19 -30.
-
Morgan, C.A. and Vesey, G. (eds.), 2009. Freeze-drying of microorganisms. Encyclopedia of Microbiology, 3rd Ed. Elsevier, New York, NY, USA, pp. 162-173.
Encyclopedia of Microbiology , () 162 -173.
-
Morgan, C.A., Herman, N., White, P.A. and Vesey, G., 2006. Preservation of micro-organisms by drying: a review. Journal of Microbiological Methods 66: 183-193.
'Preservation of micro-organisms by drying: a review ' () 66 Journal of Microbiological Methods : 183 -193.
-
Pehkonen, K.S., Roos, Y.H., Miao, S., Ross, R.P. and Stanton, C., 2008. State transitions and physicochemical aspects of cryoprotection and stabilization in freeze-drying of Lactobacillus rhamnosus GG (LGG). Journal of Applied Microbiology 104: 1732-1743.
'State transitions and physicochemical aspects of cryoprotection and stabilization in freeze-drying of Lactobacillus rhamnosus GG (LGG) ' () 104 Journal of Applied Microbiology : 1732 -1743.
-
Poddar, D., Das, S., Jones, G., Palmer, J., Jameson, G.B., Haverkamp, R.G. and Singh, H., 2014. Stability of probiotic Lactobacillus paracasei during storage as affected by the drying method. International Dairy Journal 39: 1-7.
'Stability of probiotic Lactobacillus paracasei during storage as affected by the drying method ' () 39 International Dairy Journal : 1 -7.
-
Poddar, D., Nag, A., Das, S. and Singh, H., 2012. Stabilization of probiotics for industrial application. In: Ghosh, D., Das, S., Bagchi, D. and Smarta, R.B. (eds.) Innovation in healthy and functional foods. CRC Press, Boca-Raton, FL, USA, pp. 269-304.
Innovation in healthy and functional foods , () 269 -304.
-
Rubio, R., Jofré, A., Aymerich, T., Guàrdia, M.D. and Garriga, M., 2014a. Nutritionally enhanced fermented sausages as a vehicle for potential probiotic lactobacilli delivery. Meat Science 96: 937-942.
'Nutritionally enhanced fermented sausages as a vehicle for potential probiotic lactobacilli delivery ' () 96 Meat Science : 937 -942.
-
Rubio, R., Jofré, A., Martín, B., Aymerich, T. and Garriga, M., 2014b. Characterization of lactic acid bacteria isolated from infant faeces as potential probiotic starter cultures for fermented sausages. Food Microbiology 38: 303-311.
'Characterization of lactic acid bacteria isolated from infant faeces as potential probiotic starter cultures for fermented sausages ' () 38 Food Microbiology : 303 -311.
-
Rubio, R., Martín, B., Aymerich, T. and Garriga, M., 2014c. The potential probiotic Lactobacillus rhamnosus CTC1679 survives the passage through the gastrointestinal tract and its use as starter culture results in safe nutritionally enhanced fermented sausages. International Journal of Food Microbiology 186: 55-60.
'The potential probiotic Lactobacillus rhamnosus CTC1679 survives the passage through the gastrointestinal tract and its use as starter culture results in safe nutritionally enhanced fermented sausages ' () 186 International Journal of Food Microbiology : 55 -60.
-
Santivarangkna, C., Kulozik, U. and Foerst, P., 2008. Inactivation mechanisms of lactic acid starter cultures preserved by drying processes. Journal of Applied Microbiology 105: 1-13.
'Inactivation mechanisms of lactic acid starter cultures preserved by drying processes ' () 105 Journal of Applied Microbiology : 1 -13.
-
Savini, M., Cecchini, C., Verdenelli, M.C., Silvi, S., Orpianesi, C. and Cresci, A., 2010. Pilot-scale production and viability analysis of freeze-dried probiotic bacteria using different protective agents. Nutrients 2: 330-339.
'Pilot-scale production and viability analysis of freeze-dried probiotic bacteria using different protective agents ' () 2 Nutrients : 330 -339.
-
Selmer-Olsen, E., Birkeland, S.E. and Sørhaug, T., 1999. Effect of protective solutes on leakage from and survival of immobilized Lactobacillus subjected to drying, storage and rehydration. Journal of Applied Microbiology 87: 429-437.
'Effect of protective solutes on leakage from and survival of immobilized Lactobacillus subjected to drying, storage and rehydration ' () 87 Journal of Applied Microbiology : 429 -437.
-
Strasser, S., Neureiter, M., Geppl, M., Braun, R. and Danner, H., 2009. Influence of lyophilization, fluidized bed drying, addition of protectants, and storage on the viability of lactic acid bacteria. Journal of Applied Microbiology 107: 167-177.
'Influence of lyophilization, fluidized bed drying, addition of protectants, and storage on the viability of lactic acid bacteria ' () 107 Journal of Applied Microbiology : 167 -177.
-
Tripathi, M.K. and Giri, S.K., 2014. Probiotic functional foods: survival of probiotics during processing and storage. Journal of Functional Foods 9: 225-241.
'Probiotic functional foods: survival of probiotics during processing and storage ' () 9 Journal of Functional Foods : 225 -241.
-
Ulmer, H.M., Gänzle, M.G. and Vogel, R.F., 2000. Effects of high pressure on survival and metabolic activity of Lactobacillus plantarum TMW 1.460. Applied and Environmental Microbiology 66: 3966-3973.
'Effects of high pressure on survival and metabolic activity of Lactobacillus plantarum TMW 1.460 ' () 66 Applied and Environmental Microbiology : 3966 -3973.
-
Zamora, L.M., Carretero, C. and Parés, D., 2006. Comparative survival rates of lactic acid bacteria isolated from blood, following spray-drying and freeze-drying. Food Science and Technology International 12: 77-84.
'Comparative survival rates of lactic acid bacteria isolated from blood, following spray-drying and freeze-drying ' () 12 Food Science and Technology International : 77 -84.
-
Zayed, G. and Roos, Y.H., 2004. Influence of trehalose and moisture content on survival of Lactobacillus salivarius subjected to freeze-drying and storage. Process Biochemistry 39: 1081-1086.
'Influence of trehalose and moisture content on survival of Lactobacillus salivarius subjected to freeze-drying and storage ' () 39 Process Biochemistry : 1081 -1086.
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Impact of different cryoprotectants on the survival of freeze-dried Lactobacillus rhamnosus and Lactobacillus casei/paracasei during long-term storage
In: Beneficial MicrobesSearch for other papers by A. Jofré in
Current site
Google Scholar
PubMed
Search for other papers by T. Aymerich in
Current site
Google Scholar
PubMed
Search for other papers by M. Garriga in
Current site
Google Scholar
PubMed
The production of long shelf-life highly concentrated dried probiotic/starter cultures is of paramount importance for the food industry. The aim of the present study was to evaluate the protective effect of glucose, lactose, trehalose, and skim milk applied alone or combined upon the survival of potentially probiotic Lactobacillus rhamnosus CTC1679, Lactobacillus casei/paracasei CTC1677 and L. casei/paracasei CTC1678 during freeze-drying and after 39 weeks of storage at 4 and 22 °C. Immediately after freeze-drying, the percentage of survivors was very high (≥94%) and only slight differences were observed among strains and cryoprotectants. In contrast, during storage, survival in the dried state depended on the cryoprotectant, temperature and strain. For all the protectants assayed, the stability of the cultures was remarkably higher when stored under refrigeration (4 °C). Under these conditions, skim milk alone or supplemented with trehalose or lactose showed the best performance (reductions ≤0.9 log units after 39 weeks of storage). The lowest survival was observed during non-refrigerated storage and with glucose and glucose plus milk; no viable cells left at the end of the storage period. Thus, freeze-drying in the presence of appropriate cryoprotectants allows the production of long shelf-life highly concentrated dried cultures ready for incorporation in high numbers into food products as starter/potential probiotic cultures.
| All Time | Past 365 days | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 1496 | 569 | 51 |
| Full Text Views | 81 | 22 | 0 |
| PDF Views & Downloads | 101 | 26 | 0 |
