Four weeks supplementation with Lactobacillus paracasei subsp. paracasei L. casei W8® shows modest effect on triacylglycerol in young healthy adults
In: Beneficial MicrobesSearch for other papers by A.T. Bjerg in
Current site
Google Scholar
Search for other papers by M. Kristensen in
Current site
Google Scholar
Search for other papers by C. Ritz in
Current site
Google Scholar
Search for other papers by K.D. Stark in
Current site
Google Scholar
Search for other papers by J.J. Holst in
Current site
Google Scholar
Search for other papers by T.D. Leser in
Current site
Google Scholar
Search for other papers by A. Wellejus in
Current site
Google Scholar
Search for other papers by A. Astrup in
Current site
Google Scholar
The microbiota has been shown to have the potential to affect appetite and blood lipids positively in animal studies. We investigated if four weeks supplementation with Lactobacillus paracasei subsp. paracasei L. casei W8® (L. casei W8) had an effect on subjective appetite sensation, ad libitum energy intake, glucagon-like peptide 1 (GLP-1), glucose and insulin response in humans. Secondarily, we explored potential effects on blood lipids, fatty acids and stearoyl-CoA desaturase-1 (SCD1) activity in humans as well as SCD1 expression in piglets given L. casei W8 for two weeks. 64 healthy participants completed the double-blinded, randomised, controlled, parallel four weeks study with supplementation of L. casei W8 (1010 cfu) or placebo capsules. A meal test was conducted before and after the intervention, where subjective appetite, ad libitum energy intake, GLP-1, glucose and insulin response were measured. Additionally fasting blood lipids and fatty acids concentrations were measured. Sixteen piglets were randomised into two groups: L. casei W8 (1010 cfu/day) as top dressing on morning fed or no treatment. After two weeks piglets were sacrificed and tissue from ileum, jejunum and skeletal muscle were sampled for mRNA analyses of SCD1 expression. Compared to placebo, L. casei W8 did not affect appetite, ad libitum energy intake, GLP-1, glucose and insulin response and total, high-density or low-density lipoprotein cholesterol levels after four weeks intervention. Triacylglycerol decreased in the L. casei W8 group compared to placebo at week 4 (P=0.03). The C16:1n-7/C16:0 ratio, reflecting SCD1 activity, tended to decrease when having L. casei W8 (P=0.06) compared to placebo. Muscle SCD1 expression decreased in piglets supplemented with L. casei W8 compared to control. In conclusion, supplementation with L. casei W8 did not affect appetite parameters, glucose or insulin responses; but appear to be able to lower triacylglycerol levels, possibly by reducing its production.
Purchase
Buy instant access (PDF download and unlimited online access):
Institutional Login
Log in with Open Athens, Shibboleth, or your institutional credentials
Personal login
Log in with your brill.com account
-
Attie, A.D., Krauss, R.M., Gray-Keller, M.P., Brownlie, A., Miyazaki, M., Kastelein, J.J., Lusis, A.J., Stalenhoef, A.F., Stoehr, J.P., Hayden, M.R. and Ntambi, J.M., 2002. Relationship between stearoyl-CoA desaturase activity and plasma triglycerides in human and mouse hypertriglyceridemia. Journal of Lipid Research 43: 1899-1907.
'Relationship between stearoyl-CoA desaturase activity and plasma triglycerides in human and mouse hypertriglyceridemia ' () 43 Journal of Lipid Research : 1899 -1907.
-
Bjerg, A.T., Kristensen, M., Ritz, C., Holst, J.J., Rasmussen, C., Leser, T.D., Wellejus, A. and Astrup, A., 2014. Lactobacillus paracasei subsp paracasei L. casei W8 suppresses energy intake acutely. Appetite 82: 111-118.
'Lactobacillus paracasei subsp paracasei L. casei W8 suppresses energy intake acutely ' () 82 Appetite : 111 -118.
-
Brown, J.M., Chung, S., Sawyer, J.K., Degirolamo, C., Alger, H.M., Nguyen, T., Zhu, X., Duong, M.N., Wibley, A.L., Shah, R., Davis, M.A., Kelley, K., Wilson, M.D., Kent, C., Parks, J.S. and Rudel, L.L., 2008. Inhibition of stearoyl-coenzyme A desaturase 1 dissociates insulin resistance and obesity from atherosclerosis. Circulation 118: 1467-1475.
'Inhibition of stearoyl-coenzyme A desaturase 1 dissociates insulin resistance and obesity from atherosclerosis ' () 118 Circulation : 1467 -1475.
-
Cani, P.D., Dewever, C. and Delzenne, N.M., 2004. Inulin-type fructans modulate gastrointestinal peptides involved in appetite regulation (glucagon-like peptide-1 and ghrelin) in rats. British Journal of Nutrition 92: 521-526.
'Inulin-type fructans modulate gastrointestinal peptides involved in appetite regulation (glucagon-like peptide-1 and ghrelin) in rats ' () 92 British Journal of Nutrition : 521 -526.
-
Cani, P.D., Neyrinck, A.M., Maton, N. and Delzenne, N.M., 2005. Oligofructose promotes satiety in rats fed a high-fat diet: involvement of glucagon-like peptide-1. Obesity Research 13: 1000-1007.
'Oligofructose promotes satiety in rats fed a high-fat diet: involvement of glucagon-like peptide-1 ' () 13 Obesity Research : 1000 -1007.
-
Chiang, S.S. and Pan, T.M., 2012. Beneficial effects of Lactobacillus paracasei subsp. paracasei NTU 101 and its fermented products. Applied Microbiology and Biotechnology 93: 903-916.
'Beneficial effects of Lactobacillus paracasei subsp. paracasei NTU 101 and its fermented products ' () Applied Microbiology and Biotechnology .
-
Christie, W., 2003. Lipid analysis. The Oily Press, Bridgewater, UK.
Lipid analysis , ().
-
Cohen, P., Miyazaki, M., Socci, N.D., Hagge-Greenberg, A., Liedtke, W., Soukas, A.A., Sharma, R., Hudgins, L.C., Ntambi, J.M. and Friedman, J.M., 2002. Role for stearoyl-CoA desaturase-1 in leptin-mediated weight loss. Science 297: 240-243.
'Role for stearoyl-CoA desaturase-1 in leptin-mediated weight loss ' () 297 Science : 240 -243.
-
Food and Agriculture Organization of the United Nations/World Health Organization (FAO/WHO), 2001. Evaluation of health and nutritional properties of powder milk and live lactic acid bacteria. Available at: http://www.who.int/foodsafety/publications/fs_management/en/probiotics.pdf
-
Flint, A., Raben, A., Blundell, J.E. and Astrup, A., 2000. Reproducibility, power and validity of visual analogue scales in assessment of appetite sensations in single test meal studies. International Journal of Obesity Related Metabolic Disorders 24: 38-48.
'Reproducibility, power and validity of visual analogue scales in assessment of appetite sensations in single test meal studies ' () 24 International Journal of Obesity Related Metabolic Disorders : 38 -48.
-
Folch, J., Lees, M. and Sloane Stanley, G.H., 1957. A simple method for the isolation and purification of total lipides from animal tissues. Journal of Biological Chemistry 226: 497-509.
'A simple method for the isolation and purification of total lipides from animal tissues ' () 226 Journal of Biological Chemistry : 497 -509.
-
Forssten, S.D., Korczynska, M.Z., Zwijsen, R.M., Noordman, W.H., Madetoja, M. and Ouwehand, A.C., 2013. Changes in satiety hormone concentrations and feed intake in rats in response to lactic acid bacteria. Appetite 71C: 16-21.
'Changes in satiety hormone concentrations and feed intake in rats in response to lactic acid bacteria ' () 71C Appetite : 16 -21.
-
Friedewald, W.T., Levy, R.I. and Fredrickson, D.S., 1972. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clinincal Chemistry 18: 499-502.
'Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge ' () 18 Clinincal Chemistry : 499 -502.
-
Fuentes, M.C., Lajo, T., Carrion, J.M. and Cune, J., 2013. Cholesterol-lowering efficacy of Lactobacillus plantarum CECT 7527, 7528 and 7529 in hypercholesterolaemic adults. British Journal of Nutrition 109: 1866-1872.
'Cholesterol-lowering efficacy of Lactobacillus plantarum CECT 7527, 7528 and 7529 in hypercholesterolaemic adults ' () 109 British Journal of Nutrition : 1866 -1872.
-
Georg, J.M., Kristensen, M. and Astrup, A., 2012. Effect of alginate supplementation on weight loss in obese subjects completing a 12-wk energy-restricted diet: a randomized controlled trial. Amercian Journal of Clinical Nutrition 96: 5-13.
'Effect of alginate supplementation on weight loss in obese subjects completing a 12-wk energy-restricted diet: a randomized controlled trial ' () 96 Amercian Journal of Clinical Nutrition : 5 -13.
-
Ji, Y.S., Kim, H.N., Park, H.J., Lee, J.E., Yeo, S.Y., Yang, J.S., Park, S.Y., Yoon, H.S., Cho, G.S., Franz, C.M., Bomba, A., Shin, H.K. and Holzapfel, W.H., 2012. Modulation of the murine microbiome with a concomitant anti-obesity effect by Lactobacillus rhamnosus GG and Lactobacillus sakei NR28. Beneficial Microbes 3: 13-22.
'Modulation of the murine microbiome with a concomitant anti-obesity effect by Lactobacillus rhamnosus GG and Lactobacillus sakei NR28 ' () 3 Beneficial Microbes : 13 -22.
-
Metherel, A.H., Armstrong, J.M., Patterson, A.C. and Stark, K.D., 2009a. Assessment of blood measures of n-3 polyunsaturated fatty acids with acute fish oil supplementation and washout in men and women. Prostaglandins, Leukotrienes and Essential Fatty Acids 81: 23-29.
'Assessment of blood measures of n-3 polyunsaturated fatty acids with acute fish oil supplementation and washout in men and women ' () 81 Prostaglandins, Leukotrienes and Essential Fatty Acids : 23 -29.
-
Metherel, A.H., Buzikievich, L.M., Charkhzarin, P., Patterson, A.C., Peel, A.C., Howorth, A.M., Kishi, D.M. and Stark, K.D., 2012. Omega-3 polyunsaturated fatty acid profiling using fingertip-prick whole blood does not require overnight fasting before blood collection. Nutrition Research 32: 547-556.
'Omega-3 polyunsaturated fatty acid profiling using fingertip-prick whole blood does not require overnight fasting before blood collection ' () 32 Nutrition Research : 547 -556.
-
Metherel, A.H., Taha, A.Y., Izadi, H. and Stark, K.D., 2009b. The application of ultrasound energy to increase lipid extraction throughput of solid matrix samples (flaxseed). Prostaglandins, Leukotrienes and Essential Fatty Acids 81: 417-423.
'The application of ultrasound energy to increase lipid extraction throughput of solid matrix samples (flaxseed) ' () 81 Prostaglandins, Leukotrienes and Essential Fatty Acids : 417 -423.
-
Million, M., Angelakis, E., Paul, M., Armougom, F., Leibovici, L. and Raoult, D., 2012a. Comparative meta-analysis of the effect of Lactobacillus species on weight gain in humans and animals. Microbial Pathogens 53: 100-108.
'Comparative meta-analysis of the effect of Lactobacillus species on weight gain in humans and animals ' () 53 Microbial Pathogens : 100 -108.
-
Million, M., Maraninchi, M., Henry, M., Armougom, F., Richet, H., Carrieri, P., Valero, R., Raccah, D., Vialettes, B. and Raoult, D., 2012b. Obesity-associated gut microbiota is enriched in Lactobacillus reuteri and depleted in Bifidobacterium animalis and Methanobrevibacter smithii. International Journal of Obesity 36: 817-825.
'Obesity-associated gut microbiota is enriched in Lactobacillus reuteri and depleted in Bifidobacterium animalis and Methanobrevibacter smithii ' () 36 International Journal of Obesity : 817 -825.
-
Miyazaki, M., Flowers, M.T., Sampath, H., Chu, K., Otzelberger, C., Liu, X. and Ntambi, J.M., 2007. Hepatic stearoyl-CoA desaturase-1 deficiency protects mice from carbohydrate-induced adiposity and hepatic steatosis. Cell Metab 6: 484-496.
'Hepatic stearoyl-CoA desaturase-1 deficiency protects mice from carbohydrate-induced adiposity and hepatic steatosis ' () 6 Cell Metab : 484 -496.
-
Miyazaki, M., Sampath, H., Liu, X., Flowers, M.T., Chu, K., Dobrzyn, A. and Ntambi, J.M., 2009. Stearoyl-CoA desaturase-1 deficiency attenuates obesity and insulin resistance in leptin-resistant obese mice. Biochemical and Biophysical Research Communications 380: 818-822.
'Stearoyl-CoA desaturase-1 deficiency attenuates obesity and insulin resistance in leptin-resistant obese mice ' () 380 Biochemical and Biophysical Research Communications : 818 -822.
-
Morrison, W.R. and Smith, L.M., 1964. Preparation of fatty acid methyl esters and dimethylacetals from lipids with boron fluoride-methanol. Journal of Lipid Research 5: 600-608.
'Preparation of fatty acid methyl esters and dimethylacetals from lipids with boron fluoride-methanol ' () 5 Journal of Lipid Research : 600 -608.
-
Ntambi, J.M., Miyazaki, M., Stoehr, J.P., Lan, H., Kendziorski, C.M., Yandell, B.S., Song, Y., Cohen, P., Friedman, J.M. and Attie, A.D., 2002. Loss of stearoyl-CoA desaturase-1 function protects mice against adiposity. Proceedings of the National Academy of Sciences of the USA 99: 11482-11486.
'Loss of stearoyl-CoA desaturase-1 function protects mice against adiposity ' () 99 Proceedings of the National Academy of Sciences of the USA : 11482 -11486.
-
O'Hara, A.M. and Shanahan, F., 2006. The gut flora as a forgotten organ. EMBO Reports 7: 688-693.
'The gut flora as a forgotten organ ' () 7 EMBO Reports : 688 -693.
-
Ockene, I.S., Chiriboga, D.E., Stanek, E.J., III, Harmatz, M.G., Nicolosi, R., Saperia, G., Well, A.D., Freedson, P., Merriam, P.A., Reed, G., Ma, Y., Matthews, C.E. and Hebert, J.R., 2004. Seasonal variation in serum cholesterol levels: treatment implications and possible mechanisms. Archives of Internal Medicine 164: 863-870.
'Seasonal variation in serum cholesterol levels: treatment implications and possible mechanisms ' () 164 Archives of Internal Medicine : 863 -870.
-
Orskov, C., Rabenhoj, L., Wettergren, A., Kofod, H. and Holst, J.J., 1994. Tissue and plasma concentrations of amidated and glycine-extended glucagon-like peptide I in humans. Diabetes 43: 535-539.
'Tissue and plasma concentrations of amidated and glycine-extended glucagon-like peptide I in humans ' () 43 Diabetes : 535 -539.
-
Paillard, F., Catheline, D., Duff, F.L., Bouriel, M., Deugnier, Y., Pouchard, M., Daubert, J.C. and Legrand, P., 2008. Plasma palmitoleic acid, a product of stearoyl-coA desaturase activity, is an independent marker of triglyceridemia and abdominal adiposity. Nutrition, Metabolism and Cardiovascular Diseases 18: 436-440.
'Plasma palmitoleic acid, a product of stearoyl-coA desaturase activity, is an independent marker of triglyceridemia and abdominal adiposity ' () 18 Nutrition, Metabolism and Cardiovascular Diseases : 436 -440.
-
Parnell, J.A. and Reimer, R.A., 2012. Prebiotic fiber modulation of the gut microbiota improves risk factors for obesity and the metabolic syndrome. Gut Microbes. 3: 29-34.
'Prebiotic fiber modulation of the gut microbiota improves risk factors for obesity and the metabolic syndrome ' () 3 Gut Microbes : 29 -34.
-
Sadrzadeh-Yeganeh, H., Elmadfa, I., Djazayery, A., Jalali, M., Heshmat, R. and Chamary, M., 2010. The effects of probiotic and conventional yoghurt on lipid profile in women. British Journal of Nutrition 103: 1778-1783.
'The effects of probiotic and conventional yoghurt on lipid profile in women ' () 103 British Journal of Nutrition : 1778 -1783.
-
Sampath, H., Miyazaki, M., Dobrzyn, A. and Ntambi, J.M., 2007. Stearoyl-CoA desaturase-1 mediates the pro-lipogenic effects of dietary saturated fat. Journal of Biological Chemistry. 282: 2483-2493.
'Stearoyl-CoA desaturase-1 mediates the pro-lipogenic effects of dietary saturated fat ' () 282 Journal of Biological Chemistry : 2483 -2493.
-
Stsepetova, J., Sepp, E., Kolk, H., Loivukene, K., Songisepp, E. and Mikelsaar, M., 2011. Diversity and metabolic impact of intestinal Lactobacillus species in healthy adults and the elderly. British Journal of Nutrition 105: 1235-1244.
'Diversity and metabolic impact of intestinal Lactobacillus species in healthy adults and the elderly ' () 105 British Journal of Nutrition : 1235 -1244.
-
Turnbaugh, P.J., Backhed, F., Fulton, L. and Gordon, J.I., 2008. Diet-induced obesity is linked to marked but reversible alterations in the mouse distal gut microbiome. Cell Host Microbes 3: 213-223.
'Diet-induced obesity is linked to marked but reversible alterations in the mouse distal gut microbiome ' () 3 Cell Host Microbes : 213 -223.
-
Turnbaugh, P.J., Ley, R.E., Hamady, M., Fraser-Liggett, C.M., Knight, R. and Gordon, J.I., 2007. The human microbiome project. Nature 449: 804-810.
'The human microbiome project ' () 449 Nature : 804 -810.
-
Van Baarlen, P., Troost, F., Van der Meer, C., Hooiveld, G., Boekschoten, M., Brummer, R.J. and Kleerebezem, M., 2011. Human mucosal in vivo transcriptome responses to three lactobacilli indicate how probiotics may modulate human cellular pathways. Proceedings of the National Academy of Sciences of the USA 108 Suppl. 1: 4562-4569.
'Human mucosal in vivo transcriptome responses to three lactobacilli indicate how probiotics may modulate human cellular pathways ' () 108 Proceedings of the National Academy of Sciences of the USA : 4562 -4569.
| All Time | Past 365 days | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 796 | 227 | 12 |
| Full Text Views | 20 | 2 | 1 |
| PDF Views & Downloads | 19 | 2 | 0 |
Four weeks supplementation with Lactobacillus paracasei subsp. paracasei L. casei W8® shows modest effect on triacylglycerol in young healthy adults
In: Beneficial MicrobesSearch for other papers by A.T. Bjerg in
Current site
Google Scholar
PubMed
Search for other papers by M. Kristensen in
Current site
Google Scholar
PubMed
Search for other papers by C. Ritz in
Current site
Google Scholar
PubMed
Search for other papers by K.D. Stark in
Current site
Google Scholar
PubMed
Search for other papers by J.J. Holst in
Current site
Google Scholar
PubMed
Search for other papers by T.D. Leser in
Current site
Google Scholar
PubMed
Search for other papers by A. Wellejus in
Current site
Google Scholar
PubMed
Search for other papers by A. Astrup in
Current site
Google Scholar
PubMed
The microbiota has been shown to have the potential to affect appetite and blood lipids positively in animal studies. We investigated if four weeks supplementation with Lactobacillus paracasei subsp. paracasei L. casei W8® (L. casei W8) had an effect on subjective appetite sensation, ad libitum energy intake, glucagon-like peptide 1 (GLP-1), glucose and insulin response in humans. Secondarily, we explored potential effects on blood lipids, fatty acids and stearoyl-CoA desaturase-1 (SCD1) activity in humans as well as SCD1 expression in piglets given L. casei W8 for two weeks. 64 healthy participants completed the double-blinded, randomised, controlled, parallel four weeks study with supplementation of L. casei W8 (1010 cfu) or placebo capsules. A meal test was conducted before and after the intervention, where subjective appetite, ad libitum energy intake, GLP-1, glucose and insulin response were measured. Additionally fasting blood lipids and fatty acids concentrations were measured. Sixteen piglets were randomised into two groups: L. casei W8 (1010 cfu/day) as top dressing on morning fed or no treatment. After two weeks piglets were sacrificed and tissue from ileum, jejunum and skeletal muscle were sampled for mRNA analyses of SCD1 expression. Compared to placebo, L. casei W8 did not affect appetite, ad libitum energy intake, GLP-1, glucose and insulin response and total, high-density or low-density lipoprotein cholesterol levels after four weeks intervention. Triacylglycerol decreased in the L. casei W8 group compared to placebo at week 4 (P=0.03). The C16:1n-7/C16:0 ratio, reflecting SCD1 activity, tended to decrease when having L. casei W8 (P=0.06) compared to placebo. Muscle SCD1 expression decreased in piglets supplemented with L. casei W8 compared to control. In conclusion, supplementation with L. casei W8 did not affect appetite parameters, glucose or insulin responses; but appear to be able to lower triacylglycerol levels, possibly by reducing its production.
| All Time | Past 365 days | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 796 | 227 | 12 |
| Full Text Views | 20 | 2 | 1 |
| PDF Views & Downloads | 19 | 2 | 0 |
