Save

Inhibition of Staphylococcus aureus by crude and fractionated extract from lactic acid bacteria

In: Beneficial Microbes
Authors:
C.-B. Wong School of Industrial Technology, Universiti Sains Malaysia, 11800, Penang, Malaysia

Search for other papers by C.-B. Wong in
Current site
Google Scholar
PubMed
Close
,
B.-Y. Khoo Institute for Research in Molecular Medicine, Universiti Sains Malaysia, 11800, Penang, Malaysia

Search for other papers by B.-Y. Khoo in
Current site
Google Scholar
PubMed
Close
,
S. Sasidharan Institute for Research in Molecular Medicine, Universiti Sains Malaysia, 11800, Penang, Malaysia

Search for other papers by S. Sasidharan in
Current site
Google Scholar
PubMed
Close
,
W. Piyawattanametha

Search for other papers by W. Piyawattanametha in
Current site
Google Scholar
PubMed
Close
,
S.H. Kim Division of Food Bioscience and Technology, Korea University, Seoul 136-701, South Korea

Search for other papers by S.H. Kim in
Current site
Google Scholar
PubMed
Close
,
N. Khemthongcharoen

Search for other papers by N. Khemthongcharoen in
Current site
Google Scholar
PubMed
Close
,
M.-Y. Ang Analytical Department, Fisher Scientific Sdn Bhd, Shah Alam, 40400, Selangor, Malaysia

Search for other papers by M.-Y. Ang in
Current site
Google Scholar
PubMed
Close
,
L.-O. Chuah School of Industrial Technology, Universiti Sains Malaysia, 11800, Penang, Malaysia

Search for other papers by L.-O. Chuah in
Current site
Google Scholar
PubMed
Close
, and
M.-T. Liong School of Industrial Technology, Universiti Sains Malaysia, 11800, Penang, Malaysia

Search for other papers by M.-T. Liong in
Current site
Google Scholar
PubMed
Close
Download Citation Get Permissions

Access options

Get access to the full article by using one of the access options below.

Institutional Login

Log in with Open Athens, Shibboleth, or your institutional credentials

Login via Institution

Purchase

Buy instant access (PDF download and unlimited online access):

€36.93

Increasing levels of antibiotic resistance by Staphyloccocus aureus have posed a need to search for non-antibiotic alternatives. This study aimed to assess the inhibitory effects of crude and fractionated cell-free supernatants (CFS) of locally isolated lactic acid bacteria (LAB) against a clinical strain of S. aureus. A total of 42 LAB strains were isolated and identified from fresh vegetables, fresh fruits and fermented products prior to evaluation of inhibitory activities. CFS of LAB strains exhibiting a stronger inhibitive effect against S. aureus were fractionated into crude protein, polysaccharide and lipid fractions. Crude protein fractions showed greater inhibition against S. aureus compared to polysaccharide and lipid fractions, with a more prevalent effect from Lactobacillus plantarum 8513 and L. plantarum BT8513. Crude protein, polysaccharide and lipid fractions were also characterised with glycine, mannose and oleic acid being detected as the major component of each fraction, respectively. Scanning electron microscopy revealed roughed and wrinkled membrane morphology of S. aureus upon treatment with crude protein fractions of LAB, suggesting an inhibitory effect via the destruction of cellular membrane. This research illustrated the potential application of fractionated extracts from LAB to inhibit S. aureus for use in the food and health industry.

Content Metrics

All Time Past 365 days Past 30 Days
Abstract Views 615 249 24
Full Text Views 25 5 1
PDF Views & Downloads 11 0 0