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Bifidobacterium infantis has a beneficial effect on 5-fluorouracil-induced intestinal mucositis in rats

In: Beneficial Microbes
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K.-T. Yuan Department of General Surgery, The First Affiliated Hospital, Sun Yat-sen University, No. 58 Zhongshan 2nd Road, 510080 Guangzhou, China P.R.

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H.-L. Yu Department of General Surgery, The First Affiliated Hospital, Sun Yat-sen University, No. 58 Zhongshan 2nd Road, 510080 Guangzhou, China P.R.

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W.-D. Feng Department of General Surgery, The First Affiliated Hospital, Sun Yat-sen University, No. 58 Zhongshan 2nd Road, 510080 Guangzhou, China P.R.

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P. Chong Department of Oral and Maxillofacial Surgery, Shanghai Ninth People–s Hospital affiliated to Shanghai Jiao Tong University School of Medicine, 639 Zhizaoju Road, Huangpu, 200011 Shanghai, China P.R.

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T. Yang Department of General Surgery, The First Affiliated Hospital, Sun Yat-sen University, No. 58 Zhongshan 2nd Road, 510080 Guangzhou, China P.R.

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C.-L. Xue Department of General Surgery, The First Affiliated Hospital, Sun Yat-sen University, No. 58 Zhongshan 2nd Road, 510080 Guangzhou, China P.R.

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M. Yu Department of General Surgery, The First Affiliated Hospital, Sun Yat-sen University, No. 58 Zhongshan 2nd Road, 510080 Guangzhou, China P.R.

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H.-P. Shi Department of General Surgery, The First Affiliated Hospital, Sun Yat-sen University, No. 58 Zhongshan 2nd Road, 510080 Guangzhou, China P.R.

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Intestinal mucositis is a common toxic side effect in cancer patients receiving high-dose chemotherapy. This study aimed to evaluate the beneficial effects of Bifidobacterium infantis in a rat model of intestinal mucositis induced by 5-fluorouracil (5-FU). Thirty male Sprague-Dawley rats were divided into three groups: control, 5-FU, and 5-FU + B. infantis. A single intraperitoneal injection of 5-FU (150 mg/kg) was used to induce intestinal mucositis. B. infantis (1×109 cfu) was administered for 11 days, starting from 7 days before 5-FU injection. Intestinal mucositis was evaluated based on body weight, villus height, immunohistological expression of proliferating cell nuclear antigen (PCNA) and nuclear factor kappa beta (NF-κB), levels of the pro-inflammatory factors interleukin 1 beta and tumour necrosis factor alpha, and myeloperoxidase (MPO) concentration. The results showed that the 5-FU + B. infantis group demonstrated a higher body weight and villus height, increased expression of PCNA, reduced expression of NF-κB and pro-inflammatory factors, and lower MPO concentration compared to the 5-FU group. These data suggest that probiotic B. infantis is effective in reducing chemotherapy-induced intestinal mucositis in rats.

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