The survival of Lactobacillus delbrueckii UFV H2b20 was assessed in fermented milk, both during the storage period and after exposure to simulated gastric and intestinal juices, as well the detection of the gene fbpA involved in adherence to human gastrointestinal tract. L. delbrueckii UFV H2b20 remained stable and viable for 28 days under refrigerated storage conditions. After one day of storage, that strain exhibited a one-log population reduction following exposure in tandem to simulated gastric and intestinal juices. After 14 days of storage, a two-log reduction was observed following 90 min of exposure to the simulated gastric conditions. However, the strain did not survive following exposure to the simulated intestinal juice. The observed tolerance to storage conditions and resistance to the simulated gastric and intestinal conditions confirm the potential use of L. delbrueckii UFV H2b20 as a probiotic, which is further reinforced by the detection of fbpA in this strain.
Purchase
Buy instant access (PDF download and unlimited online access):
Institutional Login
Log in with Open Athens, Shibboleth, or your institutional credentials
Personal login
Log in with your brill.com account
Altermann, E., Russell, W.M., Azcarate-Peril, M.A., Barrangou, R., Buck, B.L., McAuliffe, O., Dobson, A., Callanan, M., Lick, S., Hamrick, A., Cano, R. and Klaenhammer, T.R., 2005. Complete genome sequence of the probiotic lactic acid bacterium Lactobacillus acidophilus NCFM. Proceedings of the National Academy of Sciences of the USA 102: 3906-3912.
'Complete genome sequence of the probiotic lactic acid bacterium Lactobacillus acidophilus NCFM ' () 102 Proceedings of the National Academy of Sciences of the USA : 3906 -3912.
Araújo, E.A., Carvalho, A.F., Leandro, E.S., Furtado, M.M. and Moraes, C.A., 2009. Produção de queijo tipo cottage simbiótico e estudo de sobrevivência das células probióticas quando expostas a diferentes estresses. Pesquisa Agropecuária Tropical 39: 111-118.
'Produção de queijo tipo cottage simbiótico e estudo de sobrevivência das células probióticas quando expostas a diferentes estresses ' () 39 Pesquisa Agropecuária Tropical : 111 -118.
Azcarate-Peril, M.A., Alterman, E., Goh, Y.J., Tallon, R., Sanozky- Dawes, R.B., Pfeiler, E.A., O’Flaherty, S., Buck, B.L., Dobson, A., Duong, T., Miller, M.J., Barrangou, R. and Klaenhammer, T.R., 2008. Analysis of the genome sequence of Lactobacillus gasseri ATCC 33323 reveals the molecular basis of an autochthonous intestinal organism. Applied and Environmental Microbiology 74: 4610-4625.
'Analysis of the genome sequence of Lactobacillus gasseri ATCC 33323 reveals the molecular basis of an autochthonous intestinal organism ' () 74 Applied and Environmental Microbiology : 4610 -4625.
Bron, P.A. and Kleerebezem, M., 2011. Engineering lactic acid bacteria for increased industrial functionality. Bioengineered Bugs 2: 80-87.
'Engineering lactic acid bacteria for increased industrial functionality ' () 2 Bioengineered Bugs : 80 -87.
Buck, B.L., Altermann, E., Svingerud, T. and Klaenhammer, T.R., 2005. Functional analysis of putative adhesion factors in Lactobacillus acidophilus NCFM. Applied and Environmental Microbiology 71: 8344-8351.
'Functional analysis of putative adhesion factors in Lactobacillus acidophilus NCFM ' () 71 Applied and Environmental Microbiology : 8344 -8351.
Dos Santos Leandro, E., De Araújo, E.A., Da Conceição, L.L., De Moraes, C.A. and De Carvalho, A.F., 2013. Survival of Lactobacillus delbrueckii UFV H2b20 in ice cream produced with different fat levels and after submission to stress acid and bile salts. Journal of Functional Foods 5: 503-507.
'Survival of Lactobacillus delbrueckii UFV H2b20 in ice cream produced with different fat levels and after submission to stress acid and bile salts ' () 5 Journal of Functional Foods : 503 -507.
Kankainen, M., Paulin, L., Tynkkynen, S., Von Ossowski, I., Reunanen, J., Partanen, P., Satokari, R., Vesterlund, S., Hendrickx, A.P.A., Lebeer, S., De Keersmaecker, S.C.J., Vanderleyden, J., Hämäläinen, T., Laukkanen, S., Salovuori, N., Ritari, J., Alatalo, E., Korpela, R., Mattila-Sandholm, T., Lassig, A., Hatakka, K., Kinnunen, K.T., Karjalainen, H., Saxelin, M., Laakso, K., Surakka, A., Palva, A., Salusjärvi, T., Auvinen, P. and De Vos, W.M., 2009. Comparative genomic analysis of Lactobacillus rhamnosus GG reveals pili containing a human-mucus binding protein. Proceedings of the National Academy of Sciences of the USA 106: 17193-17198.
'Comparative genomic analysis of Lactobacillus rhamnosus GG reveals pili containing a human-mucus binding protein ' () 106 Proceedings of the National Academy of Sciences of the USA : 17193 -17198.
Kleerebezem, M., Boekhorst. J., Van Kranenburg, R., Molenaar, D., Kuipers, O.P., Leer, R., Tarchini, R., Peters, S.A., Sandbrink, H.M., Fiers, M.W.E.J., Stiekema, W., Lankhorst, R.M.K., Bron, P.A., Hoffer, S.M., Groot, M.N.N., Kerkhoven, R., Vries, M., Ursing, B., De Vos, W.M. and Siezen, R.J., 2003. Complete genome sequence of Lactobacillus plantarum WCFS1. Proceedings of the National Academy of Sciences of the USA 4: 1990-1995.
'Complete genome sequence of Lactobacillus plantarum WCFS1 ' () 4 Proceedings of the National Academy of Sciences of the USA : 1990 -1995.
Leverrier, P., Fremont, Y., Rouault, A., Boyaval, P. and Jan, G., 2005. In vitro tolerance to digestive stresses of propionibacteria: influence of food matrices. Food Microbiology 22: 11-18.
'In vitro tolerance to digestive stresses of propionibacteria: influence of food matrices ' () 22 Food Microbiology : 11 -18.
Morelli, L., 2007. In vitro assessment of probiotic bacteria: From survival to functionality. International Dairy Journal 17: 1278-1283.
'In vitro assessment of probiotic bacteria: From survival to functionality ' () 17 International Dairy Journal : 1278 -1283.
Neumann, E., Oliveira, M.A., Cabral, C.M., Moura, L.N., Nicoli, J.R., Vieira, E.C., Cara, D.C., Podoprigora, G.I. and Vieira, L.Q., 1998. Monoassociation with Lactobacillus acidophilus UFV H2b20 stimulates the imune defense mechanisms of germfree mice. Brazilian Journal of Medical and Biological Research 31: 1565-1573.
'Monoassociation with Lactobacillus acidophilus UFV H2b20 stimulates the imune defense mechanisms of germfree mice ' () 31 Brazilian Journal of Medical and Biological Research : 1565 -1573.
Ochman, H., Gerber, A.S. and Hartl, D.L., 1988. Genetic applications of an inverse Polymerase Chain Reaction. Genetics 120: 621-623.
'Genetic applications of an inverse Polymerase Chain Reaction ' () 120 Genetics : 621 -623.
Pacheco, K.C., Toro, G.V., Martínez, F.R. and Durán-páramo, E., 2010. Viability of Lactobacillus delbrueckii under human gastrointestinal conditions simulated in vitro. American Journal of Agricultural and Biological Sciences 1: 37-42.
'Viability of Lactobacillus delbrueckii under human gastrointestinal conditions simulated in vitro. ' () 1 American Journal of Agricultural and Biological Sciences : 37 -42.
Reid, G., Sanders, M.E., Gaskins. H.R., Gibson, G.R., Mercenier, A., Rastall, R., Roberfroid, M., Rowland, I., Cherbut, C. and Klaenhammer, T.R., 2003. New scientific paradigms for probiotics and prebiotics. Journal of Clinical Gastroenterology 37: 105-118.
'New scientific paradigms for probiotics and prebiotics ' () 37 Journal of Clinical Gastroenterology : 105 -118.
Santos, L.M., Santos, M.M., Silva, H.P.S., Arantes, R.M.E., Nicoli, J.R. and Vieira, L.Q., 2011. Monoassociation with probiotic Lactobacillus delbrueckii UFV H2b20 stimulates the immune system and protects germfree mice against Listeria monocytogenes infection. Medical Microbiology Immunology 200: 29-38.
'Monoassociation with probiotic Lactobacillus delbrueckii UFV H2b20 stimulates the immune system and protects germfree mice against Listeria monocytogenes infection ' () 200 Medical Microbiology Immunology : 29 -38.
Santos, N.S., 1984. Isolamento e caracterização de Lactobacillus acidophilus de fezes de crianças alimentadas ao seio e de bezerros, visando sua utilização como adjunto dietético. MSc Thesis. Departamento de Tecnologia de Alimentos, Universidade Federal de Viçosa, Viçosa, MG, Brasil.
Isolamento e caracterização de Lactobacillus acidophilus de fezes de crianças alimentadas ao seio e de bezerros, visando sua utilização como adjunto dietético. MSc Thesis. Departamento de Tecnologia de Alimentos , ().
Wang, J., Guo, Z., Zhang, Q., Yan, L., Chen, W., Liu, X.M. and Zhang, H.P., 2009. Fermentation characteristics and transit tolerance of probiotic Lactobacillus casei Zhang in soymilk and bovine milk during storage. Journal of Dairy Science 92: 2468-2476.
'Fermentation characteristics and transit tolerance of probiotic Lactobacillus casei Zhang in soymilk and bovine milk during storage ' () 92 Journal of Dairy Science : 2468 -2476.
| All Time | Past 365 days | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 276 | 64 | 13 |
| Full Text Views | 7 | 0 | 0 |
| PDF Views & Downloads | 8 | 0 | 0 |
The survival of Lactobacillus delbrueckii UFV H2b20 was assessed in fermented milk, both during the storage period and after exposure to simulated gastric and intestinal juices, as well the detection of the gene fbpA involved in adherence to human gastrointestinal tract. L. delbrueckii UFV H2b20 remained stable and viable for 28 days under refrigerated storage conditions. After one day of storage, that strain exhibited a one-log population reduction following exposure in tandem to simulated gastric and intestinal juices. After 14 days of storage, a two-log reduction was observed following 90 min of exposure to the simulated gastric conditions. However, the strain did not survive following exposure to the simulated intestinal juice. The observed tolerance to storage conditions and resistance to the simulated gastric and intestinal conditions confirm the potential use of L. delbrueckii UFV H2b20 as a probiotic, which is further reinforced by the detection of fbpA in this strain.
| All Time | Past 365 days | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 276 | 64 | 13 |
| Full Text Views | 7 | 0 | 0 |
| PDF Views & Downloads | 8 | 0 | 0 |