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Survival of Lactobacillus delbrueckii UFV H2b20 in fermented milk under simulated gastric and intestinal conditions

In: Beneficial Microbes
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L.L. da Conceição Department of Microbiology, Universidade Federal de Viçosa, 36571-000 Viçosa, MG, Brazil

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E.S. Leandro Department of Microbiology, Universidade Federal de Viçosa, 36571-000 Viçosa, MG, Brazil

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F.S. Freitas Department of Microbiology, Universidade Federal de Viçosa, 36571-000 Viçosa, MG, Brazil

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M.N.V. de Oliveira Department of Microbiology, Universidade Federal de Viçosa, 36571-000 Viçosa, MG, Brazil

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A.B. Ferreira-Machado Department of Parasitology, Microbiology and Immunology, Universidade Federal de Juiz de Fora, 36.036-330 Juiz de Fora, MG, Brazil

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A.C. Borges Department of Microbiology, Universidade Federal de Viçosa, 36571-000 Viçosa, MG, Brazil

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C.A. de Moraes Department of Microbiology, Universidade Federal de Viçosa, 36571-000 Viçosa, MG, Brazil

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The survival of Lactobacillus delbrueckii UFV H2b20 was assessed in fermented milk, both during the storage period and after exposure to simulated gastric and intestinal juices, as well the detection of the gene fbpA involved in adherence to human gastrointestinal tract. L. delbrueckii UFV H2b20 remained stable and viable for 28 days under refrigerated storage conditions. After one day of storage, that strain exhibited a one-log population reduction following exposure in tandem to simulated gastric and intestinal juices. After 14 days of storage, a two-log reduction was observed following 90 min of exposure to the simulated gastric conditions. However, the strain did not survive following exposure to the simulated intestinal juice. The observed tolerance to storage conditions and resistance to the simulated gastric and intestinal conditions confirm the potential use of L. delbrueckii UFV H2b20 as a probiotic, which is further reinforced by the detection of fbpA in this strain.

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