Gram-negative bacteria account for main differences between faecal microbiota from patients with ulcerative colitis and healthy controls
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Abstract
Detailed knowledge about the composition of the intestinal microbiota may be critical to unravel the pathogenesis of ulcerative colitis (UC), a human chronic inflammatory bowel disease, since the intestinal microbes are expected to influence some of the key mechanisms involved in the inflammatory process of the gut mucosa. The aim of this study was to investigate the faecal microbiota in patients either with UC in remission (n=6) or with active disease (n=6), and in healthy controls (n=6). The composition of Gram-negative bacteria and Gram-positive bacteria was examined. Antigenic structures of Gram-negative bacteria such as lipopolysaccharides have been related to the inflammatory responses and pathogenesis of inflammatory bowel disease. Dice cluster analysis and principal component analysis of faecal microbiota profiles obtained by denaturing gradient gel electrophoresis and quantitative PCR, respectively, revealed that the composition of faecal bacteria from UC patients with active disease differed from the healthy controls and that this difference should be ascribed to Gram-negative bacteria. The analysis did not show any clear grouping of UC patients in remission. Even with the relatively low number of subjects in each group, we were able to detect a statistically significant underrepresentation of Lactobacillus spp. and Akkermansia muciniphila in UC patients with clinically active disease compared to the healthy controls. In line with previous communications, we have shown that the microbiota in UC patients with active disease differ from that in healthy controls. Our findings indicate that alterations in the composition of the Gram-negative bacterial population, as well as reduced numbers of lactobacilli and A. muciniphila may play a role in UC.
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Ahmed, S., Macfarlane, G.T., Fite, A., Mcbain, A.J., Gilbert, P. and Macfarlane, S., 2007. Mucosa-associated bacterial diversity in relation to human terminal ileum and colonic biopsy samples. Applied and Environmental Microbiology 73: 7435-7442.
'Mucosa-associated bacterial diversity in relation to human terminal ileum and colonic biopsy samples ' () 73 Applied and Environmental Microbiology : 7435 -7442.
-
Andrews, C., Griffiths, T., Kaufman, J., Vergnolle, N., Surette, M. and Rioux, K., 2011. Mesalazine (5-aminosalicylic acid) alters faecal bacterial profiles, but not mucosal proteolytic activity in diarrhoea-predominant irritable bowel syndrome. Alimentary Pharmacology and Therapeutics 34: 374-383.
'Mesalazine (5-aminosalicylic acid) alters faecal bacterial profiles, but not mucosal proteolytic activity in diarrhoea-predominant irritable bowel syndrome ' () 34 Alimentary Pharmacology and Therapeutics : 374 -383.
-
Ardizzone, S., 2003. Ulcerative colitis. Available at: http://www.orpha.net/data/patho/GB/uk-UC.pdf
-
Arumugam, M., Raes, J., Pelletier, E., Le Paslier, D., Yamada, T., Mende, D.R., Fernandes, G.R., Tap, J., Bruls, T., Batto, J.M., Bertalan, M., Borruel, N., Casellas, F., Fernandez, L., Gautier, L., Hansen, T., Hattori, M., Hayashi, T., Kleerebezem, M., Kurokawa, K., Leclerc, M., Levenez, F., Manichanh, C., Nielsen, H.B., Nielsen, T., Pons, N., Poulain, J., Qin, J.J., Sicheritz-Ponten, T., Tims, S., Torrents, D., Ugarte, E., Zoetendal, E.G., Wang, J., Guarner, F., Pedersen, O., De Vos, W.M., Brunak, S., Dore, J., Weissenbach, J., Ehrlich, S.D. and Bork, P., 2011. Enterotypes of the human gut microbiome. Nature 473: 174-180.
'Enterotypes of the human gut microbiome ' () 473 Nature : 174 -180.
-
Atarashi, K., Tanoue, T., Shima, T., Imaoka, A., Kuwahara, T., Momose, Y., Cheng, G.H., Yamasaki, S., Saito, T., Ohba, Y., Taniguchi, T., Takeda, K., Hori, S., Ivanov, I.I., Umesaki, Y., Itoh, K. and Honda, K., 2011. Induction of colonic regulatory T cells by indigenous Clostridium species. Science 331: 337-341.
'Induction of colonic regulatory T cells by indigenous Clostridium species ' () 331 Science : 337 -341.
-
Bernbom, N., Norrung, B., Saadbye, P., Molbak, L., Vogensen, F.K. and Licht, T.R., 2006. Comparison of methods and animal models commonly used for investigation of fecal microbiota: effects of time, host and gender. Journal of Microbiological Methods 66: 87-95.
'Comparison of methods and animal models commonly used for investigation of fecal microbiota: effects of time, host and gender ' () 66 Journal of Microbiological Methods : 87 -95.
-
Binder, V., 1970. A comparison between clinical state, macroscopic and microscopic appearances of rectal mucosa, and cytologic picture of mucosal exudate in ulcerative colitis. Scandinavian Journal of Gastroenterology 5: 627-632.
'A comparison between clinical state, macroscopic and microscopic appearances of rectal mucosa, and cytologic picture of mucosal exudate in ulcerative colitis ' () 5 Scandinavian Journal of Gastroenterology : 627 -632.
-
Campieri, M. and Gionchetti, P., 2001. Bacteria as the cause of ulcerative colitis. Gut 48: 132-135.
'Bacteria as the cause of ulcerative colitis ' () 48 Gut : 132 -135.
-
Codling, C., O'Mahony, L., Shanahan, F., Quigley, E.M.M. and Marchesi, J.R., 2010. A molecular analysis of fecal and mucosal bacterial communities in irritable bowel syndrome. Digestive Diseases and Sciences 55: 392-397.
'A molecular analysis of fecal and mucosal bacterial communities in irritable bowel syndrome ' () 55 Digestive Diseases and Sciences : 392 -397.
-
Collado, M.C., Derrien, M. and Isolauri, E., 2007. Intestinal integrity and Akkermansia muciniphila, a mucin-degrading member of the intestinal microbiota present in infants, adults, and the elderly. Applied and Environmental Microbiology 73: 7767-7770.
'Intestinal integrity and Akkermansia muciniphila, a mucin-degrading member of the intestinal microbiota present in infants, adults, and the elderly ' () 73 Applied and Environmental Microbiology : 7767 -7770.
-
Delroisse, J.M., Boulvin, A.L., Parmentier, I., Dauphin, R.D., Vandenbol, M. and Portetelle, D., 2008. Quantification of Bifidobacterium spp. and Lactobacillus spp. in rat fecal samples by real-time PCR. Microbiological Research 163: 663-670.
'Quantification of Bifidobacterium spp. and Lactobacillus spp. in rat fecal samples by real-time PCR ' () 163 Microbiological Research : 663 -670.
-
Denman, S.E. and McSweeney, C.S., 2006. Development of a real-time PCR assay for monitoring anaerobic fungal and cellulolytic bacterial populations within the rumen. Fems Microbiology Ecology 58: 572-582.
'Development of a real-time PCR assay for monitoring anaerobic fungal and cellulolytic bacterial populations within the rumen ' () 58 Fems Microbiology Ecology : 572 -582.
-
Derrien, M., Vaughan, E.E., Plugge, C.M. and De Vos, W.M., 2004. Akkermansia muciniphila gen. nov., sp. nov., a human intestinal mucin-degrading bacterium. International Journal of Systematic and Evolutionary Microbiology 54: 1469-1476.
'Akkermansia muciniphila gen. nov., sp. nov., a human intestinal mucin-degrading bacterium ' () 54 International Journal of Systematic and Evolutionary Microbiology : 1469 -1476.
-
Farrell, R.J. and Peppercorn, M.A., 2002. Ulcerative colitis. The Lancet 359: 331-340.
'Ulcerative colitis ' () 359 The Lancet : 331 -340.
-
Fite, A., Macfarlane, G.T., Cummings, J.H., Hopkins, M.J., Kong, S.C., Furrie, E. and Macfarlane, S., 2004. Identification and quantitation of mucosal and faecal desulfovibrios using real time polymerase chain reaction. Gut 53: 523-529.
'Identification and quantitation of mucosal and faecal desulfovibrios using real time polymerase chain reaction ' () 53 Gut : 523 -529.
-
Frank, D.N., Amand, A.L.S., Feldman, R.A., Boedeker, E.C., Harpaz, N. and Pace, N.R., 2007. Molecular-phylogenetic characterization of microbial community imbalances in human inflammatory bowel diseases. Proceedings of the National Academy of Sciences of the USA 104: 13780-13785.
'Molecular-phylogenetic characterization of microbial community imbalances in human inflammatory bowel diseases ' () 104 Proceedings of the National Academy of Sciences of the USA : 13780 -13785.
-
Grimoud, J., Durand, H., De Souza, S., Monsan, P., Ouarne, F., Theodorou, V. and Rogues, C., 2010. In vitro screening of probiotics and synbiotics according to anti-inflammatory and anti-proliferative effects. International Journal of Food Microbiology 144: 42-50.
'In vitro screening of probiotics and synbiotics according to anti-inflammatory and anti-proliferative effects ' () 144 International Journal of Food Microbiology : 42 -50.
-
Guilloteau, P., Martin, L., Eeckhaut, V., Ducatelle, R., Zabielski, R. and Van Immerseel, F., 2010. From the gut to the peripheral tissues: the multiple effects of butyrate. Nutrition Research Reviews 23: 366-384.
'From the gut to the peripheral tissues: the multiple effects of butyrate ' () 23 Nutrition Research Reviews : 366 -384.
-
Guo, X., Xia, X., Tang, R., Zhou, J., Zhao, H. and Wang, K., 2008. Development of a real-time PCR method for Firmicutes and Bacteroidetes in faeces and its application to quantify intestinal population of obese and lean pigs. Letters in Applied Microbiology 47: 367-373.
'Development of a real-time PCR method for Firmicutes and Bacteroidetes in faeces and its application to quantify intestinal population of obese and lean pigs ' () 47 Letters in Applied Microbiology : 367 -373.
-
Haarman, M. and Knol, J., 2005. Quantitative real-time PCR assays to identify and quantify fecal Bifidobacterium species in infants receiving a prebiotic infant formula. Applied and Environmental Microbiology 71: 2318-2324.
'Quantitative real-time PCR assays to identify and quantify fecal Bifidobacterium species in infants receiving a prebiotic infant formula ' () 71 Applied and Environmental Microbiology : 2318 -2324.
-
Hart, A.L., Al-Hassi, H.O., Rigby, R.J., Bell, S.J., Emmanuel, A.V., Knight, S.C., Kamm, M.A. and Stagg, A.J., 2005. Characteristics of intestinal dendritic cells in inflammatory bowel diseases. Gastroenterology 129: 50-65.
'Characteristics of intestinal dendritic cells in inflammatory bowel diseases ' () 129 Gastroenterology : 50 -65.
-
Heilig, H.G.H.J., Zoetendal, E.G., Vaughan, E.E., Marteau, P., Akkermans, A.D.L. and De Vos, W.M., 2002. Molecular diversity of Lactobacillus spp. and other lactic acid bacteria in the human intestine as determined by specific amplification of 16S ribosomal DNA. Applied and Environmental Microbiology 68: 114-123.
'Molecular diversity of Lactobacillus spp. and other lactic acid bacteria in the human intestine as determined by specific amplification of 16S ribosomal DNA ' () 68 Applied and Environmental Microbiology : 114 -123.
-
Huijsdens, X.W., Linskens, R.K., Mak, M.T., Meuwissen, S.G.M., Vandenbroucke-Grauls, C.M.J.E. and Savelkoul, P.H.M., 2002. Quantification of bacteria adherent to gastrointestinal mucosa by real-time PCR. Journal of Clinical Microbiology 40: 4423-4427.
'Quantification of bacteria adherent to gastrointestinal mucosa by real-time PCR ' () 40 Journal of Clinical Microbiology : 4423 -4427.
-
Isaacs, K. and Herfarth, H., 2008. Role of probiotic therapy in IBD. Inflammatory Bowel Diseases 14: 1597-1605.
'Role of probiotic therapy in IBD ' () 14 Inflammatory Bowel Diseases : 1597 -1605.
-
Kaufman, J., Griffiths, T.A., Surette, M.G., Ness, S. and Rioux, K.P., 2009. Effects of mesalamine (5-aminosalicylic acid) on bacterial gene expression. Inflammatory Bowel Diseases 15: 985-996.
'Effects of mesalamine (5-aminosalicylic acid) on bacterial gene expression ' () 15 Inflammatory Bowel Diseases : 985 -996.
-
Lamine, F., Eutamene, H., Fioramonti, J., Bueno, L. and Theodorou, V., 2004. Colonic responses to Lactobacillus farciminis treatment in trinitrobenzene sulphonic acid-induced colitis in rats. Scandinavian Journal of Gastroenterology 39: 1250-1258.
'Colonic responses to Lactobacillus farciminis treatment in trinitrobenzene sulphonic acid-induced colitis in rats ' () 39 Scandinavian Journal of Gastroenterology : 1250 -1258.
-
Langholz, E., Munkholm, P., Davidsen, M. and Binder, V., 1994. Course of ulcerative-colitis – analysis of changes in disease-activity over years. Gastroenterology 107: 3-11.
'Course of ulcerative-colitis – analysis of changes in disease-activity over years ' () 107 Gastroenterology : 3 -11.
-
Larsen, N., Vogensen, F.K., Van den Berg, F.W.J., Nielsen, D.S., Andreasen, A.S., Pedersen, B.K., Bu Al-Soud, W., Sorensen, S.J., Hansen, L.H. and Jakobsen, M., 2010. Gut microbiota in human adults with type 2 diabetes differs from non-diabetic adults. PloS ONE 5: 1-10.
'Gut microbiota in human adults with type 2 diabetes differs from non-diabetic adults ' () 5 PloS ONE : 1 -10.
-
Leser, T.D., Lindecrona, R.H., Jensen, T.K., Jensen, B.B. and Moller, K., 2000. Changes in bacterial community structure in the colon of pigs fed different experimental diets and after infection with Brachyspira hyodysenteriae. Applied and Environmental Microbiology 66: 3290-3296.
'Changes in bacterial community structure in the colon of pigs fed different experimental diets and after infection with Brachyspira hyodysenteriae ' () 66 Applied and Environmental Microbiology : 3290 -3296.
-
Liu, C.X., Song, Y.L., McTeague, M., Vu, A.W., Wexler, H. and Finegold, S.M., 2003. Rapid identification of the species of the Bacteroides fragilis group by multiplex PCR assays using group- and species-specific primers. Fems Microbiology Letters 222: 9-16.
'Rapid identification of the species of the Bacteroides fragilis group by multiplex PCR assays using group- and species-specific primers ' () 222 Fems Microbiology Letters : 9 -16.
-
Loftus, E.V., 2004. Clinical epidemiology of inflammatory bowel disease: incidence, prevalence, and environmental influences. Gastroenterology 126: 1504-1517.
'Clinical epidemiology of inflammatory bowel disease: incidence, prevalence, and environmental influences ' () 126 Gastroenterology : 1504 -1517.
-
Lucke, K., Miehlke, S., Jacobs, E. and Schuppler, M., 2006. Prevalence of Bacteroides and Prevotella spp. in ulcerative colitis. Journal of Medical Microbiology 55: 617-624.
'Prevalence of Bacteroides and Prevotella spp. in ulcerative colitis ' () 55 Journal of Medical Microbiology : 617 -624.
-
Matsuki, T., Watanabe, K., Fujimoto, J., Takada, T. and Tanaka, R., 2004. Use of 16S rRNA gene-targeted group-specific primers for real-time PCR analysis of predominant bacteria in human feces. Applied and Environmental Microbiology 70: 7220-7228.
'Use of 16S rRNA gene-targeted group-specific primers for real-time PCR analysis of predominant bacteria in human feces ' () 70 Applied and Environmental Microbiology : 7220 -7228.
-
Matsuki, T., Watanabe, K., Tanaka, R. and Oyaizu, H., 1998. Rapid identification of human intestinal bifidobacteria by 16S rRNA-targeted species- and group-specific primers. Fems Microbiology Letters 167: 113-121.
'Rapid identification of human intestinal bifidobacteria by 16S rRNA-targeted species- and group-specific primers ' () 167 Fems Microbiology Letters : 113 -121.
-
Ott, S.J., Musfeldt, M., Timmis, K.N., Hampe, J., Wenderoth, D.F. and Schreiber, S., 2004. In vitro alterations of intestinal bacterial microbiota in fecal samples during storage. Diagnostic Microbiology and Infectious Disease 50: 237-245.
'In vitro alterations of intestinal bacterial microbiota in fecal samples during storage ' () 50 Diagnostic Microbiology and Infectious Disease : 237 -245.
-
Peran, L., Camuesco, D., Comalada, M., Bailon, E., Henriksson, A., Xaus, J., Zarzuelo, A. and Galvez, J., 2007. A comparative study of the preventative effects exerted by three probiotics, Bifidobacterium lactis, Lactobacillus casei and Lactobacillus acidophilus, in the TNBS model of rat colitis. Journal of Applied Microbiology 103: 836-844.
'A comparative study of the preventative effects exerted by three probiotics, Bifidobacterium lactis, Lactobacillus casei and Lactobacillus acidophilus, in the TNBS model of rat colitis ' () 103 Journal of Applied Microbiology : 836 -844.
-
Peran, L., Camuesco, D., Comalada, M., Nieto, A., Concha, A., Adrio, J.L., Olivares, M., Xaus, J., Zarzuelo, A. and Galvez, J., 2006. Lactobacillus fermentum, a probiotic capable to release glutathione, prevents colonic inflammation in the TNBS model of rat colitis. International Journal of Colorectal Disease 21: 737-746.
'Lactobacillus fermentum, a probiotic capable to release glutathione, prevents colonic inflammation in the TNBS model of rat colitis ' () 21 International Journal of Colorectal Disease : 737 -746.
-
Png, C.W., Linden, S.K., Gilshenan, K.S., Zoetendal, E.G., McSweeney, C.S., Sly, L.I., McGuckin, M.A. and Florin, T.H.J., 2010. Mucolytic bacteria with increased prevalence in ibd mucosa augment in vitro utilization of mucin by other bacteria. American Journal of Gastroenterology 105: 2420-2428.
'Mucolytic bacteria with increased prevalence in ibd mucosa augment in vitro utilization of mucin by other bacteria ' () 105 American Journal of Gastroenterology : 2420 -2428.
-
Pullan, R.D., Thomas, G.A.O., Rhodes, M., Newcombe, R.G., Williams, G.T., Allen, A. and Rhodes, J., 1994. Thickness of adherent mucus gel on colonic mucosa in humans and its relevance to colitis. Gut 35: 353-359.
'Thickness of adherent mucus gel on colonic mucosa in humans and its relevance to colitis ' () 35 Gut : 353 -359.
-
Qin, J.J., Li, R.Q., Raes, J., Arumugam, M., Burgdorf, K.S., Manichanh, C., Nielsen, T., Pons, N., Levenez, F., Yamada, T., Mende, D.R., Li, J.H., Xu, J.M., Li, S.C., Li, D.F., Cao, J.J., Wang, B., Liang, H.Q., Zheng, H.S., Xie, Y.L., Tap, J., Lepage, P., Bertalan, M., Batto, J.M., Hansen, T., Le Paslier, D., Linneberg, A., Nielsen, H.B., Pelletier, E., Renault, P., Sicheritz-Ponten, T., Turner, K., Zhu, H.M., Yu, C., Li, S.T., Jian, M., Zhou, Y., Li, Y.R., Zhang, X.Q., Li, S.G., Qin, N., Yang, H.M., Wang, J., Brunak, S., Dore, J., Guarner, F., Kristiansen, K., Pedersen, O., Parkhill, J., Weissenbach, J., Bork, P., Ehrlich, S.D. and Wang, J., 2010. A human gut microbial gene catalogue established by metagenomic sequencing. Nature 464: 59-70.
'A human gut microbial gene catalogue established by metagenomic sequencing ' () 464 Nature : 59 -70.
-
Riedel, C.U., Foata, F., Philippe, D., Adolfsson, O., Eikmanns, B.J. and Blum, S., 2006. Anti-inflammatory effects of bifidobacteria by inhibition of LPS-induced NF-kappa β activation. World Journal of Gastroenterology 12: 3729-3735.
'Anti-inflammatory effects of bifidobacteria by inhibition of LPS-induced NF-kappa β activation ' () 12 World Journal of Gastroenterology : 3729 -3735.
-
Sartor, R.B., 2004. Therapeutic manipulation of the enteric microflora in inflammatory bowel diseases: antibiotics, probiotics, and prebiotics. Gastroenterology 126: 1620-1633.
'Therapeutic manipulation of the enteric microflora in inflammatory bowel diseases: antibiotics, probiotics, and prebiotics ' () 126 Gastroenterology : 1620 -1633.
-
Sartor, R.B., 2006. Mechanisms of disease: pathogenesis of Crohn's disease and ulcerative colitis. Nature Clinical Practice Gastroenterology and Hepatology 3: 390-407.
'Mechanisms of disease: pathogenesis of Crohn's disease and ulcerative colitis ' () 3 Nature Clinical Practice Gastroenterology and Hepatology : 390 -407.
-
Sartor, R.B., 2008. Therapeutic correction of bacterial dysbiosis discovered by molecular techniques. Proceedings of the National Academy of Sciences of the USA 105: 16413-16414.
'Therapeutic correction of bacterial dysbiosis discovered by molecular techniques ' () 105 Proceedings of the National Academy of Sciences of the USA : 16413 -16414.
-
Segain, J.P., De la Bletiere, D.R., Bourreille, A., Leray, V., Gervois, N., Rosales, C., Ferrier, L., Bonnet, C., Blottiere, H.M. and Galmiche, J.P., 2000. Butyrate inhibits inflammatory responses through NF kappa ? inhibition: implications for Crohn's disease. Gut 47: 397-403.
'Butyrate inhibits inflammatory responses through NF kappa ? inhibition: implications for Crohn's disease ' () 47 Gut : 397 -403.
-
Shanks, O.C., Atikovic, E., Blackwood, A.D., Lu, J.R., Noble, R.T., Domingo, J.S., Seifring, S., Sivaganesan, M. and Haugland, R.A., 2008. Quantitative PCR for detection and enumeration of genetic markers of bovine fecal pollution. Applied and Environmental Microbiology 74: 745-752.
'Quantitative PCR for detection and enumeration of genetic markers of bovine fecal pollution ' () 74 Applied and Environmental Microbiology : 745 -752.
-
Shen, J., Zhang, B.R., Wei, G.F., Pang, X., Wei, H., Li, M., Zhang, Y., Jia, W. and Zhao, L.P., 2006. Molecular profiling of the Clostridium leptum subgroup in human fecal microflora by PCR-denaturing gradient gel electrophoresis and clone library analysis. Applied and Environmental Microbiology 72: 5232-5238.
'Molecular profiling of the Clostridium leptum subgroup in human fecal microflora by PCR-denaturing gradient gel electrophoresis and clone library analysis ' () 72 Applied and Environmental Microbiology : 5232 -5238.
-
Sokol, H., Pigneur, B., Watterlot, L., Lakhdari, O., Bermudez-Humaran, L.G., Gratadoux, J.J., Blugeon, S., Bridonneau, C., Furet, J.P., Corthier, G., Grangette, C., Vasquez, N., Pochart, P., Trugnan, G., Thomas, G., Blottiere, H.M., Dore, J., Marteau, P., Seksik, P. and Langella, P., 2008. Faecalibacterium prausnitzii is an anti-inflammatory commensal bacterium identified by gut microbiota analysis of Crohn disease patients. Proceedings of the National Academy of Sciences of the USA 105: 16731-16736.
'Faecalibacterium prausnitzii is an anti-inflammatory commensal bacterium identified by gut microbiota analysis of Crohn disease patients. ' () 105 Proceedings of the National Academy of Sciences of the USA : 16731 -16736.
-
Sokol, H., Seksik, P., Furet, J.P., Firmesse, O., Nion-Larmurier, L., Beaugerie, L., Cosnes, J., Corthier, G., Marteau, P. and Dore, J., 2009. Low counts of Faecalibacterium prausnitzii in colitis microbiota. Inflammatory Bowel Diseases 15: 1183-1189.
'Low counts of Faecalibacterium prausnitzii in colitis microbiota ' () 15 Inflammatory Bowel Diseases : 1183 -1189.
-
Swidsinski, A., Loening-Baucke, V., Bengmark, S., Lochs, H. and Dorffel, Y., 2007. Azathioprine and mesalazine-induced effects on the mucosal flora in patients with IBD colitis. Inflammatory Bowel Diseases 13: 51-56.
'Azathioprine and mesalazine-induced effects on the mucosal flora in patients with IBD colitis ' () 13 Inflammatory Bowel Diseases : 51 -56.
-
Takaishi, H., Matsuki, T., Nakazawa, A., Takada, T., Kado, S., Asahara, T., Kamada, N., Sakuraba, A., Yajima, T., Higuchi, H., Nagamu, I., Ogata, H., Iwao, Y., Nomoto, K., Tanaka, R. and Hibi, T., 2008. Imbalance in intestinal microflora constitution could be involved in the pathogenesis of inflammatory bowel disease. International Journal of Medical Microbiology 298: 463-472.
'Imbalance in intestinal microflora constitution could be involved in the pathogenesis of inflammatory bowel disease ' () 298 International Journal of Medical Microbiology : 463 -472.
-
Van Passel, M.W.J., Kant, R., Zoetendal, E.G., Plugge, C.M., Derrien, M., Malfatti, S.A., Chain, P.S.G., Woyke, T., Palva, A., De Vos, W.M. and Smidt, H., 2011. The genome of Akkermansia muciniphila, a dedicated intestinal mucin degrader, and its use in exploring intestinal metagenomes. PLoS ONE 6: e16876.
-
Vanderpool, C., Yan, F. and Polk, D.B., 2008. Mechanisms of probiotic action: implications for therapeutic applications in inflammatory bowel diseases. Inflammatory Bowel Diseases 14: 1585-1596.
'Mechanisms of probiotic action: implications for therapeutic applications in inflammatory bowel diseases ' () 14 Inflammatory Bowel Diseases : 1585 -1596.
-
Walter, J., Tannock, G.W., Tilsala-Timisjarvi, A., Rodtong, S., Loach, D.M., Munro, K. and Alatossava, T., 2000. Detection and identification of gastrointestinal Lactobacillus species by using denaturing gradient gel electrophoresis and species-specific PCR primers. Applied and Environmental Microbiology 66: 297-303.
'Detection and identification of gastrointestinal Lactobacillus species by using denaturing gradient gel electrophoresis and species-specific PCR primers ' () 66 Applied and Environmental Microbiology : 297 -303.
-
Wu, X.K., Ma, C.F., Han, L., Nawaz, M., Gao, F., Zhang, X.Y., Yu, P.B., Zhao, C.A., Li, L.C., Zhou, A.P., Wang, J.A., Moore, J.E., Millar, B.C. and Xu, J.R., 2010. Molecular characterisation of the faecal microbiota in patients with type II diabetes. Current Microbiology 61: 69-78.
'Molecular characterisation of the faecal microbiota in patients with type II diabetes ' () 61 Current Microbiology : 69 -78.
-
Zeuthen, L.H., Fink, L.N., Metzdorff, S.B., Kristensen, M.B., Licht, T.R., Nellemann, C. and Frokiaer, H., 2010. Lactobacillus acidophilus induces a slow but more sustained chemokine and cytokine response in naïve foetal enterocytes compared to commensal Escherichia coli. Bmc Immunology 11: 2.
-
Zhou, J.S., Gopal, P.K. and Gill, H.S., 2001. Potential probiotic lactic acid bacteria Lactobacillus rhamnosus (HN001), Lactobacillus acidophilus (HN017) and Bifidobacterium lactis (HN019) do not degrade gastric mucin in vitro. International Journal of Food Microbiology 63: 81-90.
'Potential probiotic lactic acid bacteria Lactobacillus rhamnosus (HN001), Lactobacillus acidophilus (HN017) and Bifidobacterium lactis (HN019) do not degrade gastric mucin in vitro ' () 63 International Journal of Food Microbiology : 81 -90.
-
Zocco, M.A., Dal Verme, L.Z., Cremonini, F., Piscaglia, A.C., Nista, E.C., Candelli, M., Novi, M., Rigante, D., Cazzato, I.A., Ojetti, V., Armuzzi, A., Gasbarrini, G. and Gasbarrini, A., 2006. Efficacy of Lactobacillus GG in maintaining remission of ulcerative colitis. Alimentary Pharmacology and Therapeutics 23: 1567-1574.
'Efficacy of Lactobacillus GG in maintaining remission of ulcerative colitis ' () 23 Alimentary Pharmacology and Therapeutics : 1567 -1574.
-
Zoetendal, E.G., Vaughan, E.E. and De Vos, W.M., 2006. A microbial world within us. Molecular Microbiology 59: 1639-1650.
'A microbial world within us ' () 59 Molecular Microbiology : 1639 -1650.
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Gram-negative bacteria account for main differences between faecal microbiota from patients with ulcerative colitis and healthy controls
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PubMed
Abstract
Detailed knowledge about the composition of the intestinal microbiota may be critical to unravel the pathogenesis of ulcerative colitis (UC), a human chronic inflammatory bowel disease, since the intestinal microbes are expected to influence some of the key mechanisms involved in the inflammatory process of the gut mucosa. The aim of this study was to investigate the faecal microbiota in patients either with UC in remission (n=6) or with active disease (n=6), and in healthy controls (n=6). The composition of Gram-negative bacteria and Gram-positive bacteria was examined. Antigenic structures of Gram-negative bacteria such as lipopolysaccharides have been related to the inflammatory responses and pathogenesis of inflammatory bowel disease. Dice cluster analysis and principal component analysis of faecal microbiota profiles obtained by denaturing gradient gel electrophoresis and quantitative PCR, respectively, revealed that the composition of faecal bacteria from UC patients with active disease differed from the healthy controls and that this difference should be ascribed to Gram-negative bacteria. The analysis did not show any clear grouping of UC patients in remission. Even with the relatively low number of subjects in each group, we were able to detect a statistically significant underrepresentation of Lactobacillus spp. and Akkermansia muciniphila in UC patients with clinically active disease compared to the healthy controls. In line with previous communications, we have shown that the microbiota in UC patients with active disease differ from that in healthy controls. Our findings indicate that alterations in the composition of the Gram-negative bacterial population, as well as reduced numbers of lactobacilli and A. muciniphila may play a role in UC.
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